East African cassava mosaic virus-Uganda2 Severe

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; East African cassava mosaic virus

Average proteome isoelectric point is 7.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9JE96|Q9JE96_9GEMI Movement protein BC1 OS=East African cassava mosaic virus-Uganda2 Severe OX=223272 GN=BC1 PE=3 SV=1
MM1 pKa = 8.03WDD3 pKa = 3.53PLVNDD8 pKa = 4.5FPEE11 pKa = 4.64TVHH14 pKa = 6.61GFRR17 pKa = 11.84SMLAVKK23 pKa = 10.21YY24 pKa = 10.06LLHH27 pKa = 7.24LEE29 pKa = 4.05QEE31 pKa = 4.43YY32 pKa = 11.26DD33 pKa = 3.21RR34 pKa = 11.84GTVGAEE40 pKa = 4.07YY41 pKa = 10.14IRR43 pKa = 11.84DD44 pKa = 3.91LIGVLRR50 pKa = 11.84CKK52 pKa = 10.67NYY54 pKa = 9.67VEE56 pKa = 3.86ATRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.31NNLNTRR67 pKa = 11.84IQGAEE72 pKa = 3.96EE73 pKa = 4.16AEE75 pKa = 4.18LRR77 pKa = 11.84QPIHH81 pKa = 6.58EE82 pKa = 4.56PCCCPHH88 pKa = 6.94CPRR91 pKa = 11.84HH92 pKa = 5.47QKK94 pKa = 10.61QNMGQQAHH102 pKa = 5.09VSEE105 pKa = 4.54TQDD108 pKa = 3.18VQNVSKK114 pKa = 10.24PRR116 pKa = 11.84CPP118 pKa = 3.47

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9ICG0|Q9ICG0_9GEMI Protein V2 OS=East African cassava mosaic virus-Uganda2 Severe OX=223272 GN=AV2 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.03SPYY27 pKa = 8.09TNRR30 pKa = 11.84VVAPTVRR37 pKa = 11.84VTRR40 pKa = 11.84SKK42 pKa = 10.08IWANRR47 pKa = 11.84PMYY50 pKa = 9.87RR51 pKa = 11.84KK52 pKa = 8.82PKK54 pKa = 7.7MYY56 pKa = 10.61RR57 pKa = 11.84MYY59 pKa = 10.67RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.23VQSFEE78 pKa = 3.87QRR80 pKa = 11.84DD81 pKa = 3.95DD82 pKa = 3.77VKK84 pKa = 11.15HH85 pKa = 6.56LGICKK90 pKa = 10.03VISDD94 pKa = 3.77VTRR97 pKa = 11.84GPGLTHH103 pKa = 6.87RR104 pKa = 11.84VGKK107 pKa = 9.86RR108 pKa = 11.84FCIKK112 pKa = 10.26SIYY115 pKa = 9.81ILGKK119 pKa = 9.09IWMDD123 pKa = 3.64EE124 pKa = 4.09NIKK127 pKa = 10.08KK128 pKa = 9.99QNHH131 pKa = 4.98TNNVMFYY138 pKa = 10.26LLRR141 pKa = 11.84DD142 pKa = 3.44RR143 pKa = 11.84RR144 pKa = 11.84PYY146 pKa = 11.11GNAPQDD152 pKa = 3.32FGQIFNMFDD161 pKa = 3.66NEE163 pKa = 4.15PSTATIKK170 pKa = 10.92NDD172 pKa = 2.93LRR174 pKa = 11.84DD175 pKa = 3.48RR176 pKa = 11.84FQVLRR181 pKa = 11.84KK182 pKa = 8.7FHH184 pKa = 5.93ATVVGGPSGMKK195 pKa = 9.47EE196 pKa = 3.54QALVKK201 pKa = 10.26RR202 pKa = 11.84FYY204 pKa = 11.15KK205 pKa = 10.49LNHH208 pKa = 6.05HH209 pKa = 5.8VTYY212 pKa = 10.81NHH214 pKa = 6.01QEE216 pKa = 3.51AGKK219 pKa = 10.41YY220 pKa = 8.39EE221 pKa = 4.09NHH223 pKa = 6.52TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.26DD253 pKa = 3.34AVTNN257 pKa = 4.16

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

940

118

307

235.0

26.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.362 ± 0.648

2.234 ± 0.515

5.106 ± 0.421

4.681 ± 0.826

3.617 ± 0.434

5.638 ± 1.12

2.872 ± 0.504

5.745 ± 0.826

6.277 ± 0.826

6.809 ± 0.519

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.38

5.426 ± 0.484

5.957 ± 0.449

3.617 ± 0.686

8.085 ± 0.506

7.872 ± 1.675

6.596 ± 0.728

6.596 ± 1.026

0.851 ± 0.051

5.106 ± 0.973
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski