Amino acid dipepetide frequency for Protochlamydia amoebophila (strain UWE25)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.39AlaAla: 4.39 ± 0.114	0.87AlaCys: 0.87 ± 0.036	2.749AlaAsp: 2.749 ± 0.08	3.839AlaGlu: 3.839 ± 0.077	3.279AlaPhe: 3.279 ± 0.079	3.9AlaGly: 3.9 ± 0.117	2.006AlaHis: 2.006 ± 0.107	5.497AlaIle: 5.497 ± 0.11	4.656AlaLys: 4.656 ± 0.098	8.254AlaLeu: 8.254 ± 0.18	1.398AlaMet: 1.398 ± 0.05	2.674AlaAsn: 2.674 ± 0.068	2.083AlaPro: 2.083 ± 0.052	3.063AlaGln: 3.063 ± 0.094	2.578AlaArg: 2.578 ± 0.069	4.173AlaSer: 4.173 ± 0.092	2.964AlaThr: 2.964 ± 0.068	3.437AlaVal: 3.437 ± 0.09	0.698AlaTrp: 0.698 ± 0.032	2.376AlaTyr: 2.376 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.709CysAla: 0.709 ± 0.034	0.278CysCys: 0.278 ± 0.023	0.625CysAsp: 0.625 ± 0.029	0.755CysGlu: 0.755 ± 0.041	0.723CysPhe: 0.723 ± 0.035	0.841CysGly: 0.841 ± 0.039	0.467CysHis: 0.467 ± 0.028	0.774CysIle: 0.774 ± 0.035	0.751CysLys: 0.751 ± 0.043	1.694CysLeu: 1.694 ± 0.058	0.217CysMet: 0.217 ± 0.018	0.461CysAsn: 0.461 ± 0.028	0.651CysPro: 0.651 ± 0.039	0.763CysGln: 0.763 ± 0.037	0.636CysArg: 0.636 ± 0.036	0.821CysSer: 0.821 ± 0.036	0.527CysThr: 0.527 ± 0.028	0.656CysVal: 0.656 ± 0.033	0.216CysTrp: 0.216 ± 0.016	0.548CysTyr: 0.548 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	3.091AspAla: 3.091 ± 0.118	0.648AspCys: 0.648 ± 0.032	1.88AspAsp: 1.88 ± 0.056	3.188AspGlu: 3.188 ± 0.078	2.603AspPhe: 2.603 ± 0.072	2.288AspGly: 2.288 ± 0.074	1.154AspHis: 1.154 ± 0.046	3.195AspIle: 3.195 ± 0.071	2.952AspLys: 2.952 ± 0.08	5.781AspLeu: 5.781 ± 0.108	0.903AspMet: 0.903 ± 0.041	1.581AspAsn: 1.581 ± 0.044	2.206AspPro: 2.206 ± 0.059	2.383AspGln: 2.383 ± 0.066	2.209AspArg: 2.209 ± 0.059	2.854AspSer: 2.854 ± 0.078	1.759AspThr: 1.759 ± 0.06	2.531AspVal: 2.531 ± 0.066	0.664AspTrp: 0.664 ± 0.033	2.004AspTyr: 2.004 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.777GluAla: 4.777 ± 0.094	0.647GluCys: 0.647 ± 0.036	2.809GluAsp: 2.809 ± 0.07	5.843GluGlu: 5.843 ± 0.138	2.822GluPhe: 2.822 ± 0.064	3.748GluGly: 3.748 ± 0.091	1.28GluHis: 1.28 ± 0.046	5.89GluIle: 5.89 ± 0.109	6.535GluLys: 6.535 ± 0.129	6.608GluLeu: 6.608 ± 0.122	1.698GluMet: 1.698 ± 0.054	3.855GluAsn: 3.855 ± 0.084	1.61GluPro: 1.61 ± 0.046	3.36GluGln: 3.36 ± 0.077	2.969GluArg: 2.969 ± 0.076	3.597GluSer: 3.597 ± 0.072	3.405GluThr: 3.405 ± 0.073	3.623GluVal: 3.623 ± 0.079	0.78GluTrp: 0.78 ± 0.039	1.733GluTyr: 1.733 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.062PheAla: 3.062 ± 0.077	0.796PheCys: 0.796 ± 0.036	2.338PheAsp: 2.338 ± 0.055	3.172PheGlu: 3.172 ± 0.067	3.048PhePhe: 3.048 ± 0.091	2.86PheGly: 2.86 ± 0.062	1.131PheHis: 1.131 ± 0.046	3.788PheIle: 3.788 ± 0.095	3.33PheLys: 3.33 ± 0.072	5.542PheLeu: 5.542 ± 0.12	0.942PheMet: 0.942 ± 0.041	2.365PheAsn: 2.365 ± 0.062	2.074PhePro: 2.074 ± 0.061	2.314PheGln: 2.314 ± 0.059	1.821PheArg: 1.821 ± 0.06	3.979PheSer: 3.979 ± 0.092	2.403PheThr: 2.403 ± 0.065	2.755PheVal: 2.755 ± 0.07	0.597PheTrp: 0.597 ± 0.033	1.995PheTyr: 1.995 ± 0.063	0.0PheXaa: 0.0 ± 0.0
Gly	3.35GlyAla: 3.35 ± 0.079	0.948GlyCys: 0.948 ± 0.041	2.252GlyAsp: 2.252 ± 0.063	3.225GlyGlu: 3.225 ± 0.087	2.756GlyPhe: 2.756 ± 0.071	3.287GlyGly: 3.287 ± 0.102	1.446GlyHis: 1.446 ± 0.047	4.874GlyIle: 4.874 ± 0.1	4.301GlyLys: 4.301 ± 0.102	6.622GlyLeu: 6.622 ± 0.169	1.425GlyMet: 1.425 ± 0.055	2.373GlyAsn: 2.373 ± 0.059	1.543GlyPro: 1.543 ± 0.047	2.201GlyGln: 2.201 ± 0.061	2.207GlyArg: 2.207 ± 0.067	3.251GlySer: 3.251 ± 0.076	2.874GlyThr: 2.874 ± 0.067	3.225GlyVal: 3.225 ± 0.081	0.774GlyTrp: 0.774 ± 0.036	2.102GlyTyr: 2.102 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.546HisAla: 1.546 ± 0.041	0.371HisCys: 0.371 ± 0.031	0.991HisAsp: 0.991 ± 0.042	1.235HisGlu: 1.235 ± 0.046	1.508HisPhe: 1.508 ± 0.048	1.097HisGly: 1.097 ± 0.044	0.803HisHis: 0.803 ± 0.037	1.629HisIle: 1.629 ± 0.049	1.145HisLys: 1.145 ± 0.04	4.678HisLeu: 4.678 ± 0.225	0.363HisMet: 0.363 ± 0.023	0.771HisAsn: 0.771 ± 0.035	1.475HisPro: 1.475 ± 0.045	1.346HisGln: 1.346 ± 0.048	1.069HisArg: 1.069 ± 0.042	1.588HisSer: 1.588 ± 0.054	1.086HisThr: 1.086 ± 0.044	1.228HisVal: 1.228 ± 0.039	0.275HisTrp: 0.275 ± 0.019	1.081HisTyr: 1.081 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	5.354IleAla: 5.354 ± 0.096	1.165IleCys: 1.165 ± 0.034	4.298IleAsp: 4.298 ± 0.075	5.693IleGlu: 5.693 ± 0.098	3.772IlePhe: 3.772 ± 0.089	4.532IleGly: 4.532 ± 0.105	1.874IleHis: 1.874 ± 0.057	4.957IleIle: 4.957 ± 0.109	4.737IleLys: 4.737 ± 0.114	8.105IleLeu: 8.105 ± 0.124	1.188IleMet: 1.188 ± 0.041	3.617IleAsn: 3.617 ± 0.076	3.693IlePro: 3.693 ± 0.073	3.89IleGln: 3.89 ± 0.083	3.2IleArg: 3.2 ± 0.072	5.539IleSer: 5.539 ± 0.099	3.502IleThr: 3.502 ± 0.081	4.117IleVal: 4.117 ± 0.095	0.712IleTrp: 0.712 ± 0.032	2.58IleTyr: 2.58 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	4.312LysAla: 4.312 ± 0.089	0.606LysCys: 0.606 ± 0.034	3.295LysAsp: 3.295 ± 0.083	6.032LysGlu: 6.032 ± 0.117	2.555LysPhe: 2.555 ± 0.067	4.079LysGly: 4.079 ± 0.095	1.585LysHis: 1.585 ± 0.049	5.955LysIle: 5.955 ± 0.102	6.485LysLys: 6.485 ± 0.137	6.695LysLeu: 6.695 ± 0.123	1.703LysMet: 1.703 ± 0.045	4.155LysAsn: 4.155 ± 0.082	2.325LysPro: 2.325 ± 0.057	3.904LysGln: 3.904 ± 0.088	3.183LysArg: 3.183 ± 0.077	4.01LysSer: 4.01 ± 0.076	4.044LysThr: 4.044 ± 0.078	3.571LysVal: 3.571 ± 0.083	0.827LysTrp: 0.827 ± 0.038	1.854LysTyr: 1.854 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	8.744LeuAla: 8.744 ± 0.177	1.264LeuCys: 1.264 ± 0.044	5.516LeuAsp: 5.516 ± 0.111	7.884LeuGlu: 7.884 ± 0.148	5.457LeuPhe: 5.457 ± 0.103	5.818LeuGly: 5.818 ± 0.121	2.338LeuHis: 2.338 ± 0.07	8.465LeuIle: 8.465 ± 0.124	9.201LeuLys: 9.201 ± 0.131	11.179LeuLeu: 11.179 ± 0.165	2.381LeuMet: 2.381 ± 0.051	6.414LeuAsn: 6.414 ± 0.152	4.563LeuPro: 4.563 ± 0.093	5.443LeuGln: 5.443 ± 0.117	4.45LeuArg: 4.45 ± 0.095	8.685LeuSer: 8.685 ± 0.141	7.717LeuThr: 7.717 ± 0.273	5.745LeuVal: 5.745 ± 0.115	1.075LeuTrp: 1.075 ± 0.044	3.22LeuTyr: 3.22 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	1.506MetAla: 1.506 ± 0.052	0.181MetCys: 0.181 ± 0.015	0.92MetAsp: 0.92 ± 0.038	1.26MetGlu: 1.26 ± 0.051	0.703MetPhe: 0.703 ± 0.034	1.458MetGly: 1.458 ± 0.048	0.543MetHis: 0.543 ± 0.031	1.681MetIle: 1.681 ± 0.057	1.43MetLys: 1.43 ± 0.05	1.88MetLeu: 1.88 ± 0.057	0.513MetMet: 0.513 ± 0.028	1.104MetAsn: 1.104 ± 0.041	0.903MetPro: 0.903 ± 0.038	1.069MetGln: 1.069 ± 0.044	0.991MetArg: 0.991 ± 0.041	1.295MetSer: 1.295 ± 0.041	1.284MetThr: 1.284 ± 0.044	1.073MetVal: 1.073 ± 0.044	0.146MetTrp: 0.146 ± 0.014	0.448MetTyr: 0.448 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.654AsnAla: 2.654 ± 0.072	0.757AsnCys: 0.757 ± 0.036	1.987AsnAsp: 1.987 ± 0.058	2.82AsnGlu: 2.82 ± 0.074	2.708AsnPhe: 2.708 ± 0.077	2.317AsnGly: 2.317 ± 0.077	1.339AsnHis: 1.339 ± 0.044	3.067AsnIle: 3.067 ± 0.077	2.801AsnLys: 2.801 ± 0.064	6.474AsnLeu: 6.474 ± 0.147	0.811AsnMet: 0.811 ± 0.043	1.97AsnAsn: 1.97 ± 0.077	2.566AsnPro: 2.566 ± 0.062	2.956AsnGln: 2.956 ± 0.086	2.206AsnArg: 2.206 ± 0.065	3.087AsnSer: 3.087 ± 0.074	2.097AsnThr: 2.097 ± 0.065	2.218AsnVal: 2.218 ± 0.062	0.659AsnTrp: 0.659 ± 0.036	1.855AsnTyr: 1.855 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	2.133ProAla: 2.133 ± 0.055	0.458ProCys: 0.458 ± 0.026	1.841ProAsp: 1.841 ± 0.057	2.634ProGlu: 2.634 ± 0.063	2.333ProPhe: 2.333 ± 0.059	1.953ProGly: 1.953 ± 0.063	1.103ProHis: 1.103 ± 0.041	3.118ProIle: 3.118 ± 0.071	2.387ProLys: 2.387 ± 0.067	5.094ProLeu: 5.094 ± 0.124	0.664ProMet: 0.664 ± 0.034	1.849ProAsn: 1.849 ± 0.056	1.455ProPro: 1.455 ± 0.067	1.742ProGln: 1.742 ± 0.055	1.222ProArg: 1.222 ± 0.041	3.291ProSer: 3.291 ± 0.077	2.091ProThr: 2.091 ± 0.058	2.054ProVal: 2.054 ± 0.057	0.456ProTrp: 0.456 ± 0.025	1.522ProTyr: 1.522 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	3.8GlnAla: 3.8 ± 0.094	0.427GlnCys: 0.427 ± 0.027	1.999GlnAsp: 1.999 ± 0.055	3.962GlnGlu: 3.962 ± 0.088	2.178GlnPhe: 2.178 ± 0.062	2.586GlnGly: 2.586 ± 0.068	1.34GlnHis: 1.34 ± 0.084	4.176GlnIle: 4.176 ± 0.088	3.976GlnLys: 3.976 ± 0.089	5.651GlnLeu: 5.651 ± 0.109	1.014GlnMet: 1.014 ± 0.039	2.517GlnAsn: 2.517 ± 0.07	1.494GlnPro: 1.494 ± 0.052	2.493GlnGln: 2.493 ± 0.064	2.029GlnArg: 2.029 ± 0.057	2.939GlnSer: 2.939 ± 0.076	2.963GlnThr: 2.963 ± 0.077	2.536GlnVal: 2.536 ± 0.066	0.513GlnTrp: 0.513 ± 0.03	1.408GlnTyr: 1.408 ± 0.051	0.0GlnXaa: 0.0 ± 0.0
Arg	2.316ArgAla: 2.316 ± 0.066	0.558ArgCys: 0.558 ± 0.028	1.861ArgAsp: 1.861 ± 0.055	3.054ArgGlu: 3.054 ± 0.069	2.199ArgPhe: 2.199 ± 0.059	2.156ArgGly: 2.156 ± 0.065	1.104ArgHis: 1.104 ± 0.049	3.155ArgIle: 3.155 ± 0.075	2.71ArgLys: 2.71 ± 0.067	5.268ArgLeu: 5.268 ± 0.103	0.946ArgMet: 0.946 ± 0.047	1.809ArgAsn: 1.809 ± 0.055	1.444ArgPro: 1.444 ± 0.051	2.265ArgGln: 2.265 ± 0.066	1.886ArgArg: 1.886 ± 0.063	2.488ArgSer: 2.488 ± 0.06	1.869ArgThr: 1.869 ± 0.06	2.137ArgVal: 2.137 ± 0.066	0.517ArgTrp: 0.517 ± 0.03	1.607ArgTyr: 1.607 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	3.485SerAla: 3.485 ± 0.075	0.889SerCys: 0.889 ± 0.042	2.916SerAsp: 2.916 ± 0.078	3.938SerGlu: 3.938 ± 0.088	3.941SerPhe: 3.941 ± 0.084	3.512SerGly: 3.512 ± 0.089	2.032SerHis: 2.032 ± 0.063	4.948SerIle: 4.948 ± 0.09	4.197SerLys: 4.197 ± 0.092	8.472SerLeu: 8.472 ± 0.123	1.318SerMet: 1.318 ± 0.049	3.031SerAsn: 3.031 ± 0.084	2.839SerPro: 2.839 ± 0.076	3.679SerGln: 3.679 ± 0.088	2.558SerArg: 2.558 ± 0.059	5.35SerSer: 5.35 ± 0.121	3.335SerThr: 3.335 ± 0.079	3.319SerVal: 3.319 ± 0.083	0.779SerTrp: 0.779 ± 0.031	2.463SerTyr: 2.463 ± 0.069	0.0SerXaa: 0.0 ± 0.0
Thr	3.256ThrAla: 3.256 ± 0.084	0.638ThrCys: 0.638 ± 0.032	2.806ThrAsp: 2.806 ± 0.131	2.843ThrGlu: 2.843 ± 0.067	2.676ThrPhe: 2.676 ± 0.066	3.0ThrGly: 3.0 ± 0.086	1.322ThrHis: 1.322 ± 0.048	4.218ThrIle: 4.218 ± 0.082	2.967ThrLys: 2.967 ± 0.087	6.256ThrLeu: 6.256 ± 0.111	0.942ThrMet: 0.942 ± 0.04	2.296ThrAsn: 2.296 ± 0.062	2.728ThrPro: 2.728 ± 0.082	2.141ThrGln: 2.141 ± 0.054	1.795ThrArg: 1.795 ± 0.048	3.586ThrSer: 3.586 ± 0.091	2.736ThrThr: 2.736 ± 0.079	2.674ThrVal: 2.674 ± 0.062	0.524ThrTrp: 0.524 ± 0.026	1.824ThrTyr: 1.824 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	3.673ValAla: 3.673 ± 0.091	0.822ValCys: 0.822 ± 0.037	2.787ValAsp: 2.787 ± 0.076	3.563ValGlu: 3.563 ± 0.082	2.562ValPhe: 2.562 ± 0.061	3.189ValGly: 3.189 ± 0.086	1.165ValHis: 1.165 ± 0.041	4.157ValIle: 4.157 ± 0.102	3.827ValLys: 3.827 ± 0.087	5.336ValLeu: 5.336 ± 0.101	1.081ValMet: 1.081 ± 0.04	2.593ValAsn: 2.593 ± 0.067	1.844ValPro: 1.844 ± 0.058	2.035ValGln: 2.035 ± 0.052	2.117ValArg: 2.117 ± 0.063	3.512ValSer: 3.512 ± 0.074	2.612ValThr: 2.612 ± 0.072	3.239ValVal: 3.239 ± 0.079	0.603ValTrp: 0.603 ± 0.03	1.723ValTyr: 1.723 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.572TrpAla: 0.572 ± 0.031	0.211TrpCys: 0.211 ± 0.022	0.493TrpAsp: 0.493 ± 0.031	0.729TrpGlu: 0.729 ± 0.034	0.461TrpPhe: 0.461 ± 0.027	0.712TrpGly: 0.712 ± 0.037	0.279TrpHis: 0.279 ± 0.021	1.042TrpIle: 1.042 ± 0.045	0.982TrpLys: 0.982 ± 0.04	1.309TrpLeu: 1.309 ± 0.054	0.324TrpMet: 0.324 ± 0.021	0.655TrpAsn: 0.655 ± 0.032	0.304TrpPro: 0.304 ± 0.022	0.574TrpGln: 0.574 ± 0.033	0.504TrpArg: 0.504 ± 0.027	0.669TrpSer: 0.669 ± 0.034	0.465TrpThr: 0.465 ± 0.026	0.624TrpVal: 0.624 ± 0.031	0.141TrpTrp: 0.141 ± 0.014	0.34TrpTyr: 0.34 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.151TyrAla: 2.151 ± 0.067	0.579TyrCys: 0.579 ± 0.037	1.509TyrAsp: 1.509 ± 0.066	1.919TyrGlu: 1.919 ± 0.056	2.027TyrPhe: 2.027 ± 0.06	1.705TyrGly: 1.705 ± 0.051	0.976TyrHis: 0.976 ± 0.042	1.942TyrIle: 1.942 ± 0.055	1.804TyrLys: 1.804 ± 0.062	4.663TyrLeu: 4.663 ± 0.103	0.555TyrMet: 0.555 ± 0.031	1.26TyrAsn: 1.26 ± 0.054	1.598TyrPro: 1.598 ± 0.052	2.381TyrGln: 2.381 ± 0.07	1.72TyrArg: 1.72 ± 0.053	2.289TyrSer: 2.289 ± 0.061	1.492TyrThr: 1.492 ± 0.055	1.678TyrVal: 1.678 ± 0.045	0.434TyrTrp: 0.434 ± 0.026	1.419TyrTyr: 1.419 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1879 proteins (644693 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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