Cyanophage 9515-10a
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3SMG9|E3SMG9_9CAUD Photosystem II reaction center protein PsbA/D1 OS=Cyanophage 9515-10a OX=444875 GN=CYOG_00043 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.35 KK3 pKa = 9.65 IALALAASIASTPAMAGPYY22 pKa = 10.58 VNVEE26 pKa = 3.99 TNADD30 pKa = 3.79 YY31 pKa = 10.66 TGSDD35 pKa = 3.56 YY36 pKa = 11.17 TSRR39 pKa = 11.84 ATDD42 pKa = 2.97 LHH44 pKa = 7.68 IGYY47 pKa = 9.8 EE48 pKa = 4.09 NEE50 pKa = 4.27 LGSLAYY56 pKa = 9.89 YY57 pKa = 9.15 VQGGKK62 pKa = 8.35 TINAADD68 pKa = 4.03 GVDD71 pKa = 3.72 SEE73 pKa = 5.85 SNFSGKK79 pKa = 10.31 LGASVSATDD88 pKa = 3.6 KK89 pKa = 10.92 LGLYY93 pKa = 10.47 GEE95 pKa = 4.47 VSFAQVEE102 pKa = 4.44 DD103 pKa = 3.74 ADD105 pKa = 3.87 NTYY108 pKa = 8.69 GTKK111 pKa = 10.52 LGAKK115 pKa = 9.94 YY116 pKa = 10.77 SFF118 pKa = 4.04
Molecular weight: 12.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.168
IPC2_protein 4.507
IPC_protein 4.38
Toseland 4.202
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.253
Rodwell 4.215
Grimsley 4.113
Solomon 4.342
Lehninger 4.291
Nozaki 4.457
DTASelect 4.66
Thurlkill 4.228
EMBOSS 4.266
Sillero 4.495
Patrickios 2.015
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.416
Protein with the highest isoelectric point:
>tr|E3SME8|E3SME8_9CAUD Predicted protein OS=Cyanophage 9515-10a OX=444875 GN=CYOG_00022 PE=4 SV=1
MM1 pKa = 7.64 APKK4 pKa = 10.13 KK5 pKa = 10.88 LPVSKK10 pKa = 10.41 LKK12 pKa = 10.74 KK13 pKa = 8.7 SARR16 pKa = 11.84 NYY18 pKa = 10.25 RR19 pKa = 11.84 KK20 pKa = 10.03 NPLSRR25 pKa = 11.84 AKK27 pKa = 10.37 KK28 pKa = 9.54 NAAQRR33 pKa = 11.84 SRR35 pKa = 11.84 NKK37 pKa = 10.57 LKK39 pKa = 10.55 INKK42 pKa = 9.45 KK43 pKa = 9.85 YY44 pKa = 10.12 RR45 pKa = 11.84 AEE47 pKa = 3.92 LNRR50 pKa = 11.84 ARR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 10.11 DD55 pKa = 3.4 GNYY58 pKa = 10.37 GKK60 pKa = 10.66 GGKK63 pKa = 9.68 DD64 pKa = 3.2 YY65 pKa = 11.44 SHH67 pKa = 7.1 TKK69 pKa = 10.04 RR70 pKa = 11.84 GTLVRR75 pKa = 11.84 EE76 pKa = 4.14 DD77 pKa = 3.41 ASKK80 pKa = 10.82 NRR82 pKa = 11.84 ARR84 pKa = 11.84 NRR86 pKa = 11.84 SKK88 pKa = 10.92 KK89 pKa = 10.27
Molecular weight: 10.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.18
IPC2_protein 10.452
IPC_protein 11.55
Toseland 11.74
ProMoST 12.12
Dawson 11.754
Bjellqvist 11.667
Wikipedia 12.164
Rodwell 11.871
Grimsley 11.798
Solomon 12.164
Lehninger 12.076
Nozaki 11.725
DTASelect 11.667
Thurlkill 11.725
EMBOSS 12.208
Sillero 11.74
Patrickios 11.579
IPC_peptide 12.164
IPC2_peptide 11.111
IPC2.peptide.svr19 8.829
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
14385
43
1708
261.5
29.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.648 ± 0.349
0.938 ± 0.195
6.597 ± 0.28
6.069 ± 0.375
3.545 ± 0.16
7.251 ± 0.599
1.696 ± 0.145
5.29 ± 0.172
6.535 ± 0.508
7.897 ± 0.338
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.377 ± 0.256
5.499 ± 0.379
4.004 ± 0.424
4.046 ± 0.329
4.463 ± 0.352
6.813 ± 0.452
6.986 ± 0.563
6.222 ± 0.292
1.369 ± 0.165
3.754 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here