Aureimonas ureilytica
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A175RRL6|A0A175RRL6_9RHIZ ABC transporter permease OS=Aureimonas ureilytica OX=401562 GN=NS365_07690 PE=4 SV=1
MM1 pKa = 7.44 SVFKK5 pKa = 10.38 STTDD9 pKa = 2.68 ATTALKK15 pKa = 9.58 FAQMAQAAYY24 pKa = 8.93 YY25 pKa = 10.74 DD26 pKa = 3.98 WMVPPAGWTAVTMGQLGLGNGSNDD50 pKa = 3.93 ASTVWHH56 pKa = 6.42 GAFGTGAQARR66 pKa = 11.84 VFTNGTEE73 pKa = 4.1 VTVAIAGTQTDD84 pKa = 4.31 NYY86 pKa = 9.73 QMGYY90 pKa = 9.87 ADD92 pKa = 3.25 WMQNVRR98 pKa = 11.84 VGQGLTDD105 pKa = 3.45 YY106 pKa = 10.5 KK107 pKa = 11.28 LNFIALVDD115 pKa = 3.75 AVKK118 pKa = 9.93 TFSEE122 pKa = 4.98 APATDD127 pKa = 3.7 LAVSFTGHH135 pKa = 6.19 SLGAAAVNQIAEE147 pKa = 4.46 SNAFGQLPSTTKK159 pKa = 10.48 YY160 pKa = 10.73 YY161 pKa = 10.91 GFEE164 pKa = 3.89 SPYY167 pKa = 10.64 VFNPPASHH175 pKa = 5.99 QAHH178 pKa = 6.34 GDD180 pKa = 3.5 VMNWGVEE187 pKa = 3.85 FDD189 pKa = 4.06 LVSGLGLGSGNPEE202 pKa = 3.92 YY203 pKa = 10.92 GQNIYY208 pKa = 10.64 LKK210 pKa = 10.22 SDD212 pKa = 3.92 FGLTSDD218 pKa = 4.65 LFTVHH223 pKa = 7.05 SIDD226 pKa = 3.43 TQVRR230 pKa = 11.84 IIEE233 pKa = 4.68 TINKK237 pKa = 9.47 SAFFTEE243 pKa = 4.78 LNTPATPNEE252 pKa = 4.24 SPSTVFITDD261 pKa = 2.98 QYY263 pKa = 11.83 GFGNHH268 pKa = 6.7 DD269 pKa = 3.88 FSGSIPMDD277 pKa = 3.51 GAYY280 pKa = 9.67 IIGSNNWEE288 pKa = 4.46 RR289 pKa = 11.84 ITGSIHH295 pKa = 7.94 DD296 pKa = 4.55 DD297 pKa = 4.05 RR298 pKa = 11.84 IDD300 pKa = 3.69 GGGGNDD306 pKa = 4.16 IIIGGAGEE314 pKa = 5.01 DD315 pKa = 3.4 IVAGGGGADD324 pKa = 3.22 TFIFSSVPVDD334 pKa = 3.22 GLPNVFSPEE343 pKa = 3.72 LDD345 pKa = 3.54 FVKK348 pKa = 10.38 DD349 pKa = 3.96 FQVMAYY355 pKa = 9.47 EE356 pKa = 4.99 GQWNWNPDD364 pKa = 2.91 TMEE367 pKa = 4.13 WVWQGGFIKK376 pKa = 10.58 DD377 pKa = 4.15 VIDD380 pKa = 3.59 LTAVRR385 pKa = 11.84 GIADD389 pKa = 3.71 FQGFAAALEE398 pKa = 4.23 DD399 pKa = 3.7 TAAGLRR405 pKa = 11.84 IEE407 pKa = 4.99 GSHH410 pKa = 5.98 DD411 pKa = 3.52 TIYY414 pKa = 10.96 LQGVTKK420 pKa = 11.02 LEE422 pKa = 4.14 MLGSFQDD429 pKa = 3.49 LGGGFMFSEE438 pKa = 4.15 NVRR441 pKa = 11.84 YY442 pKa = 9.95 NSSPYY447 pKa = 9.89 MSGNPYY453 pKa = 9.37 MSSEE457 pKa = 4.15 INQAMTGQINGYY469 pKa = 10.12 DD470 pKa = 3.99 DD471 pKa = 3.75 NTLSSEE477 pKa = 4.34 LVYY480 pKa = 11.08 SADD483 pKa = 3.52 PVNNPLGFSIASDD496 pKa = 3.73 GTYY499 pKa = 8.15 TWSYY503 pKa = 10.06 SGPPIPTDD511 pKa = 3.59 GSAYY515 pKa = 10.1 GYY517 pKa = 10.93 AEE519 pKa = 4.21 EE520 pKa = 4.24 AHH522 pKa = 5.48 VTIIDD527 pKa = 3.72 KK528 pKa = 11.09 YY529 pKa = 9.61 GAEE532 pKa = 4.23 SDD534 pKa = 3.59 VTLYY538 pKa = 9.49 GTFYY542 pKa = 10.7 SYY544 pKa = 11.6 GMAA547 pKa = 4.36
Molecular weight: 58.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 3.948
IPC_protein 3.973
Toseland 3.757
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.884
Rodwell 3.795
Grimsley 3.668
Solomon 3.961
Lehninger 3.91
Nozaki 4.062
DTASelect 4.317
Thurlkill 3.795
EMBOSS 3.897
Sillero 4.088
Patrickios 0.617
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>tr|A0A175RIC6|A0A175RIC6_9RHIZ GDP-mannose 4 6-dehydratase OS=Aureimonas ureilytica OX=401562 GN=gmd PE=3 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 SNHH5 pKa = 5.7 LRR7 pKa = 11.84 PPRR10 pKa = 11.84 GRR12 pKa = 11.84 LHH14 pKa = 6.13 RR15 pKa = 11.84 TLHH18 pKa = 6.02 RR19 pKa = 11.84 RR20 pKa = 11.84 GNPLLILHH28 pKa = 6.61 LRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LPQNPPSRR40 pKa = 11.84 PLASDD45 pKa = 3.36 RR46 pKa = 11.84 HH47 pKa = 5.68 RR48 pKa = 11.84 PSDD51 pKa = 3.31 QRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 SLTHH59 pKa = 5.35 RR60 pKa = 11.84 RR61 pKa = 11.84 SRR63 pKa = 11.84 KK64 pKa = 9.16 RR65 pKa = 11.84 PPATRR70 pKa = 11.84 QPRR73 pKa = 11.84 PPRR76 pKa = 11.84 PTT78 pKa = 3.13
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.477
IPC2_protein 11.052
IPC_protein 12.661
Toseland 12.822
ProMoST 13.32
Dawson 12.822
Bjellqvist 12.822
Wikipedia 13.305
Rodwell 12.34
Grimsley 12.866
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.062
IPC_peptide 13.334
IPC2_peptide 12.31
IPC2.peptide.svr19 9.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4329
0
4329
1349042
27
5832
311.6
33.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.812 ± 0.042
0.763 ± 0.011
5.615 ± 0.032
5.895 ± 0.037
3.816 ± 0.026
8.775 ± 0.037
1.957 ± 0.018
4.907 ± 0.028
2.878 ± 0.031
10.55 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.366 ± 0.019
2.35 ± 0.025
5.231 ± 0.034
2.983 ± 0.025
7.611 ± 0.041
5.662 ± 0.03
5.181 ± 0.022
7.367 ± 0.033
1.244 ± 0.015
2.037 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here