Pacific flying fox associated cyclovirus-1
Average proteome isoelectric point is 8.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTJ3|A0A140CTJ3_9CIRC ATP-dependent helicase Rep OS=Pacific flying fox associated cyclovirus-1 OX=1795983 PE=3 SV=1
MM1 pKa = 7.45 PGEE4 pKa = 4.51 PKK6 pKa = 9.57 SHH8 pKa = 6.0 RR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 10.77 VFTLNNYY18 pKa = 10.04 SDD20 pKa = 5.35 DD21 pKa = 3.85 DD22 pKa = 4.03 VARR25 pKa = 11.84 LQQLEE30 pKa = 3.92 ARR32 pKa = 11.84 YY33 pKa = 8.3 MVFGKK38 pKa = 10.25 EE39 pKa = 3.92 VAPTSGTKK47 pKa = 10.01 HH48 pKa = 5.3 LQGFINFGRR57 pKa = 11.84 AVRR60 pKa = 11.84 FNSARR65 pKa = 11.84 KK66 pKa = 9.12 LVGGDD71 pKa = 3.08 GVTEE75 pKa = 4.2 VTDD78 pKa = 4.23 RR79 pKa = 11.84 AASGGPNGDD88 pKa = 3.73 CFDD91 pKa = 4.35 YY92 pKa = 10.84 LPHH95 pKa = 7.02 GNDD98 pKa = 3.08 PATRR102 pKa = 11.84 GFVFLGKK109 pKa = 8.46 PTSQGRR115 pKa = 11.84 RR116 pKa = 11.84 TDD118 pKa = 3.57 LQRR121 pKa = 11.84 VADD124 pKa = 4.17 LAKK127 pKa = 10.58 QSGTTAHH134 pKa = 6.74 TIARR138 pKa = 11.84 EE139 pKa = 3.94 FPVEE143 pKa = 4.29 FIKK146 pKa = 10.89 YY147 pKa = 9.94 GRR149 pKa = 11.84 GITNLLRR156 pKa = 11.84 TINPIPSRR164 pKa = 11.84 SKK166 pKa = 8.04 PTIVVVLVGAPGVGKK181 pKa = 10.52 SRR183 pKa = 11.84 FANEE187 pKa = 3.94 VGSGLGDD194 pKa = 3.5 TYY196 pKa = 11.27 YY197 pKa = 10.95 KK198 pKa = 10.51 PRR200 pKa = 11.84 GEE202 pKa = 3.94 WWDD205 pKa = 4.56 GYY207 pKa = 9.1 CQQRR211 pKa = 11.84 SVIIDD216 pKa = 3.92 DD217 pKa = 4.33 FYY219 pKa = 11.87 GWIKK223 pKa = 10.75 YY224 pKa = 10.28 DD225 pKa = 4.1 EE226 pKa = 4.44 LLKK229 pKa = 10.69 ICDD232 pKa = 4.0 RR233 pKa = 11.84 YY234 pKa = 8.26 PHH236 pKa = 6.41 KK237 pKa = 11.26 VPVKK241 pKa = 9.99 GGYY244 pKa = 8.95 EE245 pKa = 4.19 EE246 pKa = 4.38 FTSEE250 pKa = 4.47 YY251 pKa = 11.25 VFITSNSNVDD261 pKa = 2.88 QWYY264 pKa = 9.92 KK265 pKa = 10.4 FDD267 pKa = 4.24 NYY269 pKa = 10.85 SPAAIHH275 pKa = 6.52 RR276 pKa = 11.84 RR277 pKa = 11.84 LSVYY281 pKa = 10.22 VGLMCDD287 pKa = 3.1 GSTAHH292 pKa = 6.16 FPEE295 pKa = 4.65 WSPKK299 pKa = 10.09 FSWFDD304 pKa = 3.72 SAWTTMQIKK313 pKa = 7.8 WTVHH317 pKa = 6.03 DD318 pKa = 4.31 MVNKK322 pKa = 9.82 VV323 pKa = 2.85
Molecular weight: 36.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.294
IPC2_protein 8.302
IPC_protein 8.258
Toseland 8.595
ProMoST 8.668
Dawson 9.004
Bjellqvist 8.99
Wikipedia 9.151
Rodwell 9.092
Grimsley 8.916
Solomon 9.121
Lehninger 9.077
Nozaki 9.019
DTASelect 8.873
Thurlkill 8.916
EMBOSS 9.136
Sillero 9.121
Patrickios 4.38
IPC_peptide 9.107
IPC2_peptide 7.775
IPC2.peptide.svr19 7.713
Protein with the highest isoelectric point:
>tr|A0A140CTJ3|A0A140CTJ3_9CIRC ATP-dependent helicase Rep OS=Pacific flying fox associated cyclovirus-1 OX=1795983 PE=3 SV=1
MM1 pKa = 7.49 RR2 pKa = 11.84 RR3 pKa = 11.84 MRR5 pKa = 11.84 LRR7 pKa = 11.84 RR8 pKa = 11.84 YY9 pKa = 8.76 RR10 pKa = 11.84 PRR12 pKa = 11.84 RR13 pKa = 11.84 VVAPRR18 pKa = 11.84 RR19 pKa = 11.84 GIRR22 pKa = 11.84 KK23 pKa = 8.38 RR24 pKa = 11.84 RR25 pKa = 11.84 SFRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 NRR33 pKa = 11.84 KK34 pKa = 8.41 YY35 pKa = 10.11 DD36 pKa = 3.24 VHH38 pKa = 6.88 NFRR41 pKa = 11.84 LKK43 pKa = 9.93 SGSYY47 pKa = 10.3 VVDD50 pKa = 3.85 LTPGVNGGARR60 pKa = 11.84 VVGEE64 pKa = 3.88 AVVLNQLPINFNGLTLLYY82 pKa = 10.32 SHH84 pKa = 7.11 IRR86 pKa = 11.84 ITKK89 pKa = 8.4 AVYY92 pKa = 10.12 RR93 pKa = 11.84 FIPMYY98 pKa = 7.83 TQRR101 pKa = 11.84 TWPDD105 pKa = 3.2 PATNDD110 pKa = 3.06 TDD112 pKa = 3.91 LPEE115 pKa = 4.98 ILSTQWYY122 pKa = 9.47 GPALDD127 pKa = 4.14 VDD129 pKa = 4.98 SNPSQSYY136 pKa = 9.95 NVLRR140 pKa = 11.84 SDD142 pKa = 4.12 FLTRR146 pKa = 11.84 NHH148 pKa = 5.1 QWNRR152 pKa = 11.84 PFKK155 pKa = 9.97 RR156 pKa = 11.84 VLYY159 pKa = 9.35 PKK161 pKa = 10.39 VNYY164 pKa = 9.51 FVTASTYY171 pKa = 10.69 VDD173 pKa = 3.19 NAAPNSNTACALKK186 pKa = 10.43 KK187 pKa = 9.69 MPWVRR192 pKa = 11.84 TSGSNAYY199 pKa = 10.33 DD200 pKa = 3.63 NLQCGVMYY208 pKa = 10.44 VGAIGGPAGAAVKK221 pKa = 10.48 YY222 pKa = 9.75 RR223 pKa = 11.84 VEE225 pKa = 3.6 RR226 pKa = 11.84 TYY228 pKa = 11.0 YY229 pKa = 8.42 VQARR233 pKa = 11.84 KK234 pKa = 8.87 VV235 pKa = 3.19
Molecular weight: 27.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 10.204
IPC_protein 11.111
Toseland 10.906
ProMoST 10.95
Dawson 11.023
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.038
Grimsley 11.096
Solomon 11.242
Lehninger 11.184
Nozaki 10.891
DTASelect 10.862
Thurlkill 10.935
EMBOSS 11.345
Sillero 10.979
Patrickios 10.701
IPC_peptide 11.242
IPC2_peptide 10.087
IPC2.peptide.svr19 8.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
558
235
323
279.0
31.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.272 ± 0.569
1.075 ± 0.133
5.556 ± 0.769
2.867 ± 0.941
4.48 ± 0.888
8.065 ± 1.247
1.971 ± 0.411
3.763 ± 0.464
5.018 ± 0.451
5.914 ± 0.529
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.792 ± 0.199
5.376 ± 1.099
5.914 ± 0.277
3.047 ± 0.04
9.498 ± 2.185
6.093 ± 0.332
6.272 ± 0.186
9.14 ± 0.635
2.151 ± 0.265
5.735 ± 0.635
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here