Hydrogenovibrio sp. SC-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Hydrogenovibrio; unclassified Hydrogenovibrio

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I2A705|A0A2I2A705_9GAMM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2 6-diaminopimelate ligase OS=Hydrogenovibrio sp. SC-1 OX=2065820 GN=murE PE=3 SV=1
MM1 pKa = 7.58PKK3 pKa = 9.88VHH5 pKa = 6.54VVGQGEE11 pKa = 4.87CDD13 pKa = 3.68FDD15 pKa = 4.46DD16 pKa = 4.47QFSLLDD22 pKa = 4.0ALDD25 pKa = 4.26DD26 pKa = 4.71AGFDD30 pKa = 3.36MPYY33 pKa = 9.88SCRR36 pKa = 11.84GGNCGACEE44 pKa = 4.0VKK46 pKa = 10.63LISGQVEE53 pKa = 4.88AIQEE57 pKa = 4.18PVFEE61 pKa = 4.45AEE63 pKa = 4.36KK64 pKa = 11.23GNILTCSVIPLEE76 pKa = 5.07DD77 pKa = 3.13IVIEE81 pKa = 4.13LVV83 pKa = 3.15

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I2A5L8|A0A2I2A5L8_9GAMM ATP synthase subunit c OS=Hydrogenovibrio sp. SC-1 OX=2065820 GN=atpE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.98ILAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.88GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2220

0

2220

737596

34

1758

332.3

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.449 ± 0.05

0.827 ± 0.015

5.511 ± 0.036

6.268 ± 0.043

4.13 ± 0.033

6.453 ± 0.051

2.326 ± 0.027

6.437 ± 0.044

5.498 ± 0.048

10.629 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.025

4.125 ± 0.036

4.053 ± 0.03

5.576 ± 0.052

4.311 ± 0.033

6.412 ± 0.038

5.358 ± 0.038

6.754 ± 0.054

1.249 ± 0.022

2.952 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski