Exomis microphylla associated virus
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8B2K8|A0A2I8B2K8_9GEMI V3 OS=Exomis microphylla associated virus OX=2093275 PE=4 SV=1
MM1 pKa = 7.1 VPFRR5 pKa = 11.84 VRR7 pKa = 11.84 DD8 pKa = 4.36 FPRR11 pKa = 11.84 NYY13 pKa = 9.84 PAFLAAATSCLLRR26 pKa = 11.84 YY27 pKa = 9.32 NKK29 pKa = 9.39 WFILGTLPEE38 pKa = 4.32 VTGTLTVEE46 pKa = 3.95 EE47 pKa = 4.42 QEE49 pKa = 5.42 NFIQFQKK56 pKa = 10.01 QVRR59 pKa = 11.84 KK60 pKa = 9.62 ILRR63 pKa = 11.84 SNWSFVRR70 pKa = 11.84 KK71 pKa = 9.31 CRR73 pKa = 11.84 VYY75 pKa = 10.17 EE76 pKa = 3.86 IVYY79 pKa = 8.61 TKK81 pKa = 10.93 YY82 pKa = 10.55 GGPEE86 pKa = 3.9 PEE88 pKa = 4.25 EE89 pKa = 4.86 KK90 pKa = 10.7 VPEE93 pKa = 4.21 WDD95 pKa = 3.49 PVDD98 pKa = 4.46 AEE100 pKa = 4.52 EE101 pKa = 4.81 EE102 pKa = 4.39 DD103 pKa = 4.52 EE104 pKa = 4.34 VWEE107 pKa = 4.42 VPVEE111 pKa = 4.1 EE112 pKa = 4.31 VSGEE116 pKa = 4.03 KK117 pKa = 10.36 ACEE120 pKa = 4.12 SKK122 pKa = 10.71 QGCC125 pKa = 3.79
Molecular weight: 14.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.847
IPC2_protein 4.914
IPC_protein 4.762
Toseland 4.635
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.825
Wikipedia 4.482
Rodwell 4.596
Grimsley 4.546
Solomon 4.673
Lehninger 4.622
Nozaki 4.774
DTASelect 4.825
Thurlkill 4.609
EMBOSS 4.507
Sillero 4.863
Patrickios 3.923
IPC_peptide 4.685
IPC2_peptide 4.851
IPC2.peptide.svr19 4.852
Protein with the highest isoelectric point:
>tr|A0A2I8B2L1|A0A2I8B2L1_9GEMI Replication-associated protein OS=Exomis microphylla associated virus OX=2093275 PE=3 SV=1
MM1 pKa = 7.71 AAQNLKK7 pKa = 10.36 RR8 pKa = 11.84 KK9 pKa = 8.61 SQSGTPWMRR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 8.66 KK21 pKa = 8.39 TKK23 pKa = 9.98 SGKK26 pKa = 8.01 SQWKK30 pKa = 8.7 KK31 pKa = 9.97 YY32 pKa = 8.11 PVKK35 pKa = 10.61 RR36 pKa = 11.84 PVNRR40 pKa = 11.84 SRR42 pKa = 11.84 AVKK45 pKa = 10.42 LKK47 pKa = 10.26 MYY49 pKa = 10.27 DD50 pKa = 3.43 DD51 pKa = 4.07 TFGGSGIGSTVSNDD65 pKa = 3.73 GMITMLNNYY74 pKa = 7.83 VQGIGDD80 pKa = 3.99 SQRR83 pKa = 11.84 STNVTVTKK91 pKa = 10.49 RR92 pKa = 11.84 LKK94 pKa = 10.61 FDD96 pKa = 3.45 MALMASSGFWSAPNYY111 pKa = 6.45 MAQYY115 pKa = 9.11 HH116 pKa = 5.11 WVIVDD121 pKa = 3.74 RR122 pKa = 11.84 DD123 pKa = 3.83 VGSTFPDD130 pKa = 3.26 KK131 pKa = 11.13 LDD133 pKa = 3.99 TIFDD137 pKa = 4.07 IPSQGQGMPSTYY149 pKa = 10.11 RR150 pKa = 11.84 IRR152 pKa = 11.84 RR153 pKa = 11.84 DD154 pKa = 3.1 MNEE157 pKa = 3.48 RR158 pKa = 11.84 FIVKK162 pKa = 9.97 RR163 pKa = 11.84 KK164 pKa = 4.98 WTTHH168 pKa = 4.16 MMSTGTDD175 pKa = 3.28 YY176 pKa = 11.45 GGKK179 pKa = 7.53 QTYY182 pKa = 8.75 KK183 pKa = 10.53 APSMPNYY190 pKa = 9.15 KK191 pKa = 10.01 KK192 pKa = 10.95 AMNINIRR199 pKa = 11.84 NINVKK204 pKa = 8.2 TMWKK208 pKa = 8.53 DD209 pKa = 3.19 TGGGKK214 pKa = 10.11 YY215 pKa = 10.33 EE216 pKa = 4.14 DD217 pKa = 3.64 VKK219 pKa = 11.28 EE220 pKa = 3.89 NAILYY225 pKa = 9.01 VVVNDD230 pKa = 3.71 NTDD233 pKa = 3.47 NTNMYY238 pKa = 8.08 ATLFGNCRR246 pKa = 11.84 MYY248 pKa = 10.63 FYY250 pKa = 11.43
Molecular weight: 28.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.545
IPC_protein 9.575
Toseland 10.145
ProMoST 9.823
Dawson 10.335
Bjellqvist 9.999
Wikipedia 10.496
Rodwell 10.833
Grimsley 10.409
Solomon 10.35
Lehninger 10.321
Nozaki 10.116
DTASelect 9.999
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.248
Patrickios 10.438
IPC_peptide 10.35
IPC2_peptide 8.565
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1181
89
390
236.2
27.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.98 ± 0.452
1.948 ± 0.448
5.334 ± 0.527
6.012 ± 1.794
5.08 ± 0.54
5.504 ± 0.907
2.117 ± 0.759
4.996 ± 0.447
5.673 ± 1.116
7.282 ± 1.304
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.71 ± 1.225
6.774 ± 0.732
5.758 ± 0.694
3.895 ± 0.257
6.52 ± 0.251
6.943 ± 0.479
6.266 ± 0.683
5.588 ± 1.249
2.202 ± 0.272
5.419 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here