Exomis microphylla associated virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Becurtovirus; Exomis microphylla latent virus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8B2K8|A0A2I8B2K8_9GEMI V3 OS=Exomis microphylla associated virus OX=2093275 PE=4 SV=1
MM1 pKa = 7.1VPFRR5 pKa = 11.84VRR7 pKa = 11.84DD8 pKa = 4.36FPRR11 pKa = 11.84NYY13 pKa = 9.84PAFLAAATSCLLRR26 pKa = 11.84YY27 pKa = 9.32NKK29 pKa = 9.39WFILGTLPEE38 pKa = 4.32VTGTLTVEE46 pKa = 3.95EE47 pKa = 4.42QEE49 pKa = 5.42NFIQFQKK56 pKa = 10.01QVRR59 pKa = 11.84KK60 pKa = 9.62ILRR63 pKa = 11.84SNWSFVRR70 pKa = 11.84KK71 pKa = 9.31CRR73 pKa = 11.84VYY75 pKa = 10.17EE76 pKa = 3.86IVYY79 pKa = 8.61TKK81 pKa = 10.93YY82 pKa = 10.55GGPEE86 pKa = 3.9PEE88 pKa = 4.25EE89 pKa = 4.86KK90 pKa = 10.7VPEE93 pKa = 4.21WDD95 pKa = 3.49PVDD98 pKa = 4.46AEE100 pKa = 4.52EE101 pKa = 4.81EE102 pKa = 4.39DD103 pKa = 4.52EE104 pKa = 4.34VWEE107 pKa = 4.42VPVEE111 pKa = 4.1EE112 pKa = 4.31VSGEE116 pKa = 4.03KK117 pKa = 10.36ACEE120 pKa = 4.12SKK122 pKa = 10.71QGCC125 pKa = 3.79

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8B2L1|A0A2I8B2L1_9GEMI Replication-associated protein OS=Exomis microphylla associated virus OX=2093275 PE=3 SV=1
MM1 pKa = 7.71AAQNLKK7 pKa = 10.36RR8 pKa = 11.84KK9 pKa = 8.61SQSGTPWMRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 8.66KK21 pKa = 8.39TKK23 pKa = 9.98SGKK26 pKa = 8.01SQWKK30 pKa = 8.7KK31 pKa = 9.97YY32 pKa = 8.11PVKK35 pKa = 10.61RR36 pKa = 11.84PVNRR40 pKa = 11.84SRR42 pKa = 11.84AVKK45 pKa = 10.42LKK47 pKa = 10.26MYY49 pKa = 10.27DD50 pKa = 3.43DD51 pKa = 4.07TFGGSGIGSTVSNDD65 pKa = 3.73GMITMLNNYY74 pKa = 7.83VQGIGDD80 pKa = 3.99SQRR83 pKa = 11.84STNVTVTKK91 pKa = 10.49RR92 pKa = 11.84LKK94 pKa = 10.61FDD96 pKa = 3.45MALMASSGFWSAPNYY111 pKa = 6.45MAQYY115 pKa = 9.11HH116 pKa = 5.11WVIVDD121 pKa = 3.74RR122 pKa = 11.84DD123 pKa = 3.83VGSTFPDD130 pKa = 3.26KK131 pKa = 11.13LDD133 pKa = 3.99TIFDD137 pKa = 4.07IPSQGQGMPSTYY149 pKa = 10.11RR150 pKa = 11.84IRR152 pKa = 11.84RR153 pKa = 11.84DD154 pKa = 3.1MNEE157 pKa = 3.48RR158 pKa = 11.84FIVKK162 pKa = 9.97RR163 pKa = 11.84KK164 pKa = 4.98WTTHH168 pKa = 4.16MMSTGTDD175 pKa = 3.28YY176 pKa = 11.45GGKK179 pKa = 7.53QTYY182 pKa = 8.75KK183 pKa = 10.53APSMPNYY190 pKa = 9.15KK191 pKa = 10.01KK192 pKa = 10.95AMNINIRR199 pKa = 11.84NINVKK204 pKa = 8.2TMWKK208 pKa = 8.53DD209 pKa = 3.19TGGGKK214 pKa = 10.11YY215 pKa = 10.33EE216 pKa = 4.14DD217 pKa = 3.64VKK219 pKa = 11.28EE220 pKa = 3.89NAILYY225 pKa = 9.01VVVNDD230 pKa = 3.71NTDD233 pKa = 3.47NTNMYY238 pKa = 8.08ATLFGNCRR246 pKa = 11.84MYY248 pKa = 10.63FYY250 pKa = 11.43

Molecular weight: 28.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1181

89

390

236.2

27.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.98 ± 0.452

1.948 ± 0.448

5.334 ± 0.527

6.012 ± 1.794

5.08 ± 0.54

5.504 ± 0.907

2.117 ± 0.759

4.996 ± 0.447

5.673 ± 1.116

7.282 ± 1.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 1.225

6.774 ± 0.732

5.758 ± 0.694

3.895 ± 0.257

6.52 ± 0.251

6.943 ± 0.479

6.266 ± 0.683

5.588 ± 1.249

2.202 ± 0.272

5.419 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski