Amino acid dipepetide frequency for Pelagibacter phage HTVC105P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.422AlaAla: 4.422 ± 0.785
0.457AlaCys: 0.457 ± 0.219
5.109AlaAsp: 5.109 ± 0.584
5.337AlaGlu: 5.337 ± 0.766
2.135AlaPhe: 2.135 ± 0.496
5.414AlaGly: 5.414 ± 1.127
0.686AlaHis: 0.686 ± 0.208
5.566AlaIle: 5.566 ± 0.592
6.481AlaLys: 6.481 ± 0.914
5.032AlaLeu: 5.032 ± 0.645
2.44AlaMet: 2.44 ± 0.35
6.405AlaAsn: 6.405 ± 1.359
2.059AlaPro: 2.059 ± 0.455
2.44AlaGln: 2.44 ± 0.664
1.83AlaArg: 1.83 ± 0.289
5.947AlaSer: 5.947 ± 0.771
5.414AlaThr: 5.414 ± 1.139
4.575AlaVal: 4.575 ± 0.811
0.991AlaTrp: 0.991 ± 0.292
2.745AlaTyr: 2.745 ± 0.488
0.0AlaXaa: 0.0 ± 0.0
Cys
0.457CysAla: 0.457 ± 0.181
0.076CysCys: 0.076 ± 0.075
0.534CysAsp: 0.534 ± 0.233
0.686CysGlu: 0.686 ± 0.301
0.305CysPhe: 0.305 ± 0.146
0.381CysGly: 0.381 ± 0.182
0.229CysHis: 0.229 ± 0.132
0.686CysIle: 0.686 ± 0.297
1.067CysLys: 1.067 ± 0.404
0.991CysLeu: 0.991 ± 0.349
0.229CysMet: 0.229 ± 0.114
0.457CysAsn: 0.457 ± 0.191
0.152CysPro: 0.152 ± 0.117
0.229CysGln: 0.229 ± 0.131
0.534CysArg: 0.534 ± 0.188
0.457CysSer: 0.457 ± 0.185
0.61CysThr: 0.61 ± 0.178
0.457CysVal: 0.457 ± 0.201
0.152CysTrp: 0.152 ± 0.103
0.457CysTyr: 0.457 ± 0.198
0.0CysXaa: 0.0 ± 0.0
Asp
5.032AspAla: 5.032 ± 0.742
0.534AspCys: 0.534 ± 0.247
3.202AspAsp: 3.202 ± 0.455
3.355AspGlu: 3.355 ± 0.566
1.982AspPhe: 1.982 ± 0.367
4.117AspGly: 4.117 ± 0.524
0.534AspHis: 0.534 ± 0.173
5.261AspIle: 5.261 ± 0.561
5.261AspLys: 5.261 ± 0.781
5.719AspLeu: 5.719 ± 0.553
1.525AspMet: 1.525 ± 0.423
2.44AspAsn: 2.44 ± 0.388
2.211AspPro: 2.211 ± 0.615
1.22AspGln: 1.22 ± 0.291
2.516AspArg: 2.516 ± 0.462
2.44AspSer: 2.44 ± 0.502
3.812AspThr: 3.812 ± 0.504
3.66AspVal: 3.66 ± 0.61
0.686AspTrp: 0.686 ± 0.213
2.059AspTyr: 2.059 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
4.88GluAla: 4.88 ± 0.538
1.144GluCys: 1.144 ± 0.391
3.584GluAsp: 3.584 ± 0.508
4.651GluGlu: 4.651 ± 0.601
2.135GluPhe: 2.135 ± 0.484
4.194GluGly: 4.194 ± 0.554
1.372GluHis: 1.372 ± 0.344
4.499GluIle: 4.499 ± 0.598
7.472GluLys: 7.472 ± 1.115
5.719GluLeu: 5.719 ± 0.702
2.287GluMet: 2.287 ± 0.378
3.584GluAsn: 3.584 ± 0.438
1.525GluPro: 1.525 ± 0.373
2.821GluGln: 2.821 ± 0.647
2.974GluArg: 2.974 ± 0.455
2.287GluSer: 2.287 ± 0.382
4.88GluThr: 4.88 ± 0.568
4.422GluVal: 4.422 ± 0.48
1.144GluTrp: 1.144 ± 0.351
3.05GluTyr: 3.05 ± 0.567
0.0GluXaa: 0.0 ± 0.0
Phe
1.906PheAla: 1.906 ± 0.39
0.381PheCys: 0.381 ± 0.184
3.05PheAsp: 3.05 ± 0.439
2.211PheGlu: 2.211 ± 0.423
1.144PhePhe: 1.144 ± 0.302
2.592PheGly: 2.592 ± 0.654
0.762PheHis: 0.762 ± 0.241
1.906PheIle: 1.906 ± 0.428
3.736PheLys: 3.736 ± 0.566
3.05PheLeu: 3.05 ± 0.517
1.296PheMet: 1.296 ± 0.306
2.059PheAsn: 2.059 ± 0.459
0.991PhePro: 0.991 ± 0.299
1.296PheGln: 1.296 ± 0.274
1.144PheArg: 1.144 ± 0.295
2.516PheSer: 2.516 ± 0.485
2.364PheThr: 2.364 ± 0.469
2.364PheVal: 2.364 ± 0.445
0.381PheTrp: 0.381 ± 0.128
1.296PheTyr: 1.296 ± 0.36
0.0PheXaa: 0.0 ± 0.0
Gly
4.27GlyAla: 4.27 ± 0.878
0.305GlyCys: 0.305 ± 0.135
3.507GlyAsp: 3.507 ± 0.363
4.346GlyGlu: 4.346 ± 0.501
2.745GlyPhe: 2.745 ± 0.399
4.88GlyGly: 4.88 ± 0.87
1.296GlyHis: 1.296 ± 0.311
4.041GlyIle: 4.041 ± 0.56
6.481GlyLys: 6.481 ± 0.96
4.575GlyLeu: 4.575 ± 0.608
1.296GlyMet: 1.296 ± 0.373
3.584GlyAsn: 3.584 ± 0.615
0.0GlyPro: 0.0 ± 0.0
2.135GlyGln: 2.135 ± 0.424
2.516GlyArg: 2.516 ± 0.478
4.956GlySer: 4.956 ± 0.736
6.1GlyThr: 6.1 ± 0.774
3.965GlyVal: 3.965 ± 0.472
0.839GlyTrp: 0.839 ± 0.229
2.516GlyTyr: 2.516 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
1.449HisAla: 1.449 ± 0.416
0.457HisCys: 0.457 ± 0.182
0.686HisAsp: 0.686 ± 0.165
1.144HisGlu: 1.144 ± 0.412
1.144HisPhe: 1.144 ± 0.273
0.991HisGly: 0.991 ± 0.223
0.457HisHis: 0.457 ± 0.206
1.22HisIle: 1.22 ± 0.315
0.991HisLys: 0.991 ± 0.271
1.525HisLeu: 1.525 ± 0.409
0.457HisMet: 0.457 ± 0.161
0.839HisAsn: 0.839 ± 0.219
0.534HisPro: 0.534 ± 0.174
0.61HisGln: 0.61 ± 0.221
0.61HisArg: 0.61 ± 0.236
1.83HisSer: 1.83 ± 0.371
1.22HisThr: 1.22 ± 0.291
0.762HisVal: 0.762 ± 0.254
0.381HisTrp: 0.381 ± 0.163
0.686HisTyr: 0.686 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
5.109IleAla: 5.109 ± 0.602
0.534IleCys: 0.534 ± 0.223
3.202IleAsp: 3.202 ± 0.479
5.185IleGlu: 5.185 ± 0.64
1.982IlePhe: 1.982 ± 0.401
4.27IleGly: 4.27 ± 0.588
1.296IleHis: 1.296 ± 0.309
4.575IleIle: 4.575 ± 0.716
6.634IleLys: 6.634 ± 0.688
4.651IleLeu: 4.651 ± 0.49
1.982IleMet: 1.982 ± 0.332
4.117IleAsn: 4.117 ± 0.77
2.135IlePro: 2.135 ± 0.39
1.906IleGln: 1.906 ± 0.431
2.516IleArg: 2.516 ± 0.374
4.194IleSer: 4.194 ± 0.537
4.956IleThr: 4.956 ± 0.904
2.897IleVal: 2.897 ± 0.498
0.762IleTrp: 0.762 ± 0.211
2.059IleTyr: 2.059 ± 0.423
0.0IleXaa: 0.0 ± 0.0
Lys
5.337LysAla: 5.337 ± 0.829
0.686LysCys: 0.686 ± 0.386
5.566LysAsp: 5.566 ± 0.813
8.616LysGlu: 8.616 ± 1.051
4.041LysPhe: 4.041 ± 0.551
4.422LysGly: 4.422 ± 0.561
1.83LysHis: 1.83 ± 0.338
6.252LysIle: 6.252 ± 0.674
6.939LysLys: 6.939 ± 0.944
7.549LysLeu: 7.549 ± 0.974
2.364LysMet: 2.364 ± 0.58
3.202LysAsn: 3.202 ± 0.613
3.202LysPro: 3.202 ± 0.545
3.66LysGln: 3.66 ± 0.655
4.346LysArg: 4.346 ± 0.78
4.88LysSer: 4.88 ± 0.74
5.566LysThr: 5.566 ± 0.634
5.414LysVal: 5.414 ± 0.643
0.839LysTrp: 0.839 ± 0.27
3.126LysTyr: 3.126 ± 0.714
0.0LysXaa: 0.0 ± 0.0
Leu
6.786LeuAla: 6.786 ± 0.66
0.61LeuCys: 0.61 ± 0.29
5.109LeuAsp: 5.109 ± 0.619
5.871LeuGlu: 5.871 ± 0.745
2.745LeuPhe: 2.745 ± 0.407
4.041LeuGly: 4.041 ± 0.712
1.754LeuHis: 1.754 ± 0.391
3.812LeuIle: 3.812 ± 0.474
6.329LeuLys: 6.329 ± 0.614
6.1LeuLeu: 6.1 ± 0.842
1.754LeuMet: 1.754 ± 0.423
4.727LeuAsn: 4.727 ± 0.511
3.126LeuPro: 3.126 ± 0.356
3.355LeuGln: 3.355 ± 0.635
2.897LeuArg: 2.897 ± 0.58
6.176LeuSer: 6.176 ± 0.727
5.871LeuThr: 5.871 ± 0.598
4.88LeuVal: 4.88 ± 0.571
0.839LeuTrp: 0.839 ± 0.334
2.821LeuTyr: 2.821 ± 0.391
0.0LeuXaa: 0.0 ± 0.0
Met
2.669MetAla: 2.669 ± 0.48
0.457MetCys: 0.457 ± 0.193
0.991MetAsp: 0.991 ± 0.22
1.449MetGlu: 1.449 ± 0.318
0.686MetPhe: 0.686 ± 0.243
1.525MetGly: 1.525 ± 0.347
0.381MetHis: 0.381 ± 0.157
1.449MetIle: 1.449 ± 0.387
2.135MetLys: 2.135 ± 0.387
1.982MetLeu: 1.982 ± 0.488
0.762MetMet: 0.762 ± 0.272
0.762MetAsn: 0.762 ± 0.21
1.449MetPro: 1.449 ± 0.395
0.839MetGln: 0.839 ± 0.258
1.449MetArg: 1.449 ± 0.378
2.059MetSer: 2.059 ± 0.378
1.22MetThr: 1.22 ± 0.389
1.296MetVal: 1.296 ± 0.309
0.457MetTrp: 0.457 ± 0.183
0.61MetTyr: 0.61 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
3.889AsnAla: 3.889 ± 0.606
0.381AsnCys: 0.381 ± 0.154
3.05AsnAsp: 3.05 ± 0.588
3.431AsnGlu: 3.431 ± 0.4
2.287AsnPhe: 2.287 ± 0.443
3.965AsnGly: 3.965 ± 0.789
0.381AsnHis: 0.381 ± 0.178
4.041AsnIle: 4.041 ± 0.899
4.727AsnLys: 4.727 ± 0.714
5.261AsnLeu: 5.261 ± 0.765
0.839AsnMet: 0.839 ± 0.196
3.66AsnAsn: 3.66 ± 0.565
2.059AsnPro: 2.059 ± 0.383
1.982AsnGln: 1.982 ± 0.545
2.211AsnArg: 2.211 ± 0.376
4.499AsnSer: 4.499 ± 0.93
5.642AsnThr: 5.642 ± 0.801
2.974AsnVal: 2.974 ± 0.965
0.762AsnTrp: 0.762 ± 0.278
2.287AsnTyr: 2.287 ± 0.395
0.0AsnXaa: 0.0 ± 0.0
Pro
1.754ProAla: 1.754 ± 0.381
0.229ProCys: 0.229 ± 0.129
1.982ProAsp: 1.982 ± 0.368
2.516ProGlu: 2.516 ± 0.499
1.677ProPhe: 1.677 ± 0.323
0.0ProGly: 0.0 ± 0.0
0.457ProHis: 0.457 ± 0.168
1.449ProIle: 1.449 ± 0.304
2.287ProLys: 2.287 ± 0.433
2.745ProLeu: 2.745 ± 0.469
0.762ProMet: 0.762 ± 0.21
1.601ProAsn: 1.601 ± 0.337
1.144ProPro: 1.144 ± 0.332
1.067ProGln: 1.067 ± 0.295
0.457ProArg: 0.457 ± 0.197
2.897ProSer: 2.897 ± 0.348
2.669ProThr: 2.669 ± 0.469
1.83ProVal: 1.83 ± 0.358
0.0ProTrp: 0.0 ± 0.0
1.144ProTyr: 1.144 ± 0.344
0.0ProXaa: 0.0 ± 0.0
Gln
3.279GlnAla: 3.279 ± 0.708
0.0GlnCys: 0.0 ± 0.0
1.677GlnAsp: 1.677 ± 0.259
2.059GlnGlu: 2.059 ± 0.561
1.144GlnPhe: 1.144 ± 0.329
1.677GlnGly: 1.677 ± 0.315
0.686GlnHis: 0.686 ± 0.275
2.44GlnIle: 2.44 ± 0.387
3.202GlnLys: 3.202 ± 0.518
2.974GlnLeu: 2.974 ± 0.515
0.991GlnMet: 0.991 ± 0.292
1.601GlnAsn: 1.601 ± 0.356
0.762GlnPro: 0.762 ± 0.226
1.372GlnGln: 1.372 ± 0.306
1.754GlnArg: 1.754 ± 0.412
2.821GlnSer: 2.821 ± 0.46
3.05GlnThr: 3.05 ± 0.435
2.44GlnVal: 2.44 ± 0.444
0.686GlnTrp: 0.686 ± 0.248
1.525GlnTyr: 1.525 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
2.592ArgAla: 2.592 ± 0.499
0.152ArgCys: 0.152 ± 0.11
3.05ArgAsp: 3.05 ± 0.462
2.516ArgGlu: 2.516 ± 0.36
1.22ArgPhe: 1.22 ± 0.291
2.745ArgGly: 2.745 ± 0.461
0.762ArgHis: 0.762 ± 0.268
1.906ArgIle: 1.906 ± 0.36
2.974ArgLys: 2.974 ± 0.629
3.431ArgLeu: 3.431 ± 0.683
1.22ArgMet: 1.22 ± 0.439
2.287ArgAsn: 2.287 ± 0.486
1.296ArgPro: 1.296 ± 0.289
1.449ArgGln: 1.449 ± 0.334
2.135ArgArg: 2.135 ± 0.499
1.83ArgSer: 1.83 ± 0.393
1.677ArgThr: 1.677 ± 0.335
2.364ArgVal: 2.364 ± 0.446
0.457ArgTrp: 0.457 ± 0.217
2.211ArgTyr: 2.211 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
5.566SerAla: 5.566 ± 0.951
0.61SerCys: 0.61 ± 0.215
3.584SerAsp: 3.584 ± 0.658
4.651SerGlu: 4.651 ± 0.625
3.126SerPhe: 3.126 ± 0.447
5.566SerGly: 5.566 ± 0.877
1.525SerHis: 1.525 ± 0.349
4.346SerIle: 4.346 ± 0.544
5.337SerLys: 5.337 ± 0.562
4.804SerLeu: 4.804 ± 0.496
1.144SerMet: 1.144 ± 0.28
3.965SerAsn: 3.965 ± 0.636
1.601SerPro: 1.601 ± 0.342
2.592SerGln: 2.592 ± 0.435
2.059SerArg: 2.059 ± 0.51
5.49SerSer: 5.49 ± 1.031
5.109SerThr: 5.109 ± 0.833
4.27SerVal: 4.27 ± 0.721
1.144SerTrp: 1.144 ± 0.371
2.135SerTyr: 2.135 ± 0.338
0.0SerXaa: 0.0 ± 0.0
Thr
6.939ThrAla: 6.939 ± 0.772
0.61ThrCys: 0.61 ± 0.277
3.507ThrAsp: 3.507 ± 0.493
5.032ThrGlu: 5.032 ± 0.744
2.745ThrPhe: 2.745 ± 0.545
6.252ThrGly: 6.252 ± 0.858
1.144ThrHis: 1.144 ± 0.241
4.422ThrIle: 4.422 ± 0.591
5.49ThrLys: 5.49 ± 0.713
4.88ThrLeu: 4.88 ± 0.686
1.296ThrMet: 1.296 ± 0.338
5.414ThrAsn: 5.414 ± 1.33
1.982ThrPro: 1.982 ± 0.33
3.202ThrGln: 3.202 ± 0.571
2.287ThrArg: 2.287 ± 0.396
6.176ThrSer: 6.176 ± 1.128
5.337ThrThr: 5.337 ± 1.301
4.27ThrVal: 4.27 ± 1.346
0.991ThrTrp: 0.991 ± 0.265
2.745ThrTyr: 2.745 ± 0.428
0.0ThrXaa: 0.0 ± 0.0
Val
5.947ValAla: 5.947 ± 1.496
0.762ValCys: 0.762 ± 0.285
3.584ValAsp: 3.584 ± 0.398
3.507ValGlu: 3.507 ± 0.566
1.83ValPhe: 1.83 ± 0.462
4.346ValGly: 4.346 ± 0.597
1.449ValHis: 1.449 ± 0.415
3.812ValIle: 3.812 ± 0.379
4.804ValLys: 4.804 ± 0.683
3.965ValLeu: 3.965 ± 0.614
1.372ValMet: 1.372 ± 0.336
4.575ValAsn: 4.575 ± 0.787
1.754ValPro: 1.754 ± 0.419
1.982ValGln: 1.982 ± 0.376
2.44ValArg: 2.44 ± 0.408
4.194ValSer: 4.194 ± 0.531
4.422ValThr: 4.422 ± 0.739
2.897ValVal: 2.897 ± 0.506
0.457ValTrp: 0.457 ± 0.175
1.22ValTyr: 1.22 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
0.686TrpAla: 0.686 ± 0.268
0.076TrpCys: 0.076 ± 0.074
0.686TrpAsp: 0.686 ± 0.167
0.686TrpGlu: 0.686 ± 0.242
0.305TrpPhe: 0.305 ± 0.146
0.457TrpGly: 0.457 ± 0.156
0.076TrpHis: 0.076 ± 0.073
0.61TrpIle: 0.61 ± 0.229
1.296TrpLys: 1.296 ± 0.299
1.677TrpLeu: 1.677 ± 0.411
0.152TrpMet: 0.152 ± 0.104
0.991TrpAsn: 0.991 ± 0.23
0.0TrpPro: 0.0 ± 0.0
0.61TrpGln: 0.61 ± 0.208
0.457TrpArg: 0.457 ± 0.195
0.686TrpSer: 0.686 ± 0.2
1.83TrpThr: 1.83 ± 0.397
1.144TrpVal: 1.144 ± 0.289
0.076TrpTrp: 0.076 ± 0.086
0.457TrpTyr: 0.457 ± 0.315
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.484
0.686TyrCys: 0.686 ± 0.245
1.906TyrAsp: 1.906 ± 0.374
1.372TyrGlu: 1.372 ± 0.323
1.067TyrPhe: 1.067 ± 0.362
2.516TyrGly: 2.516 ± 0.407
0.915TyrHis: 0.915 ± 0.217
2.669TyrIle: 2.669 ± 0.414
4.194TyrLys: 4.194 ± 0.772
2.974TyrLeu: 2.974 ± 0.4
0.381TyrMet: 0.381 ± 0.142
2.059TyrAsn: 2.059 ± 0.349
0.686TyrPro: 0.686 ± 0.221
1.372TyrGln: 1.372 ± 0.327
1.22TyrArg: 1.22 ± 0.376
2.287TyrSer: 2.287 ± 0.319
2.974TyrThr: 2.974 ± 0.524
2.44TyrVal: 2.44 ± 0.492
0.915TyrTrp: 0.915 ± 0.281
1.144TyrTyr: 1.144 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski