Amino acid dipepetide frequency for Gordonia phage Untouchable

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.174AlaAla: 9.174 ± 1.336
0.311AlaCys: 0.311 ± 0.113
5.131AlaAsp: 5.131 ± 0.518
5.805AlaGlu: 5.805 ± 0.5
3.732AlaPhe: 3.732 ± 0.446
6.375AlaGly: 6.375 ± 1.015
1.607AlaHis: 1.607 ± 0.36
5.39AlaIle: 5.39 ± 0.753
5.857AlaLys: 5.857 ± 0.697
6.893AlaLeu: 6.893 ± 1.233
3.006AlaMet: 3.006 ± 0.457
3.576AlaAsn: 3.576 ± 0.499
1.503AlaPro: 1.503 ± 0.323
3.317AlaGln: 3.317 ± 0.466
4.665AlaArg: 4.665 ± 0.484
4.924AlaSer: 4.924 ± 0.457
5.494AlaThr: 5.494 ± 0.663
7.463AlaVal: 7.463 ± 0.916
0.726AlaTrp: 0.726 ± 0.219
2.332AlaTyr: 2.332 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
0.311CysAla: 0.311 ± 0.141
0.0CysCys: 0.0 ± 0.0
0.311CysAsp: 0.311 ± 0.131
0.466CysGlu: 0.466 ± 0.19
0.052CysPhe: 0.052 ± 0.055
0.622CysGly: 0.622 ± 0.209
0.052CysHis: 0.052 ± 0.055
0.415CysIle: 0.415 ± 0.16
0.259CysLys: 0.259 ± 0.134
0.726CysLeu: 0.726 ± 0.182
0.104CysMet: 0.104 ± 0.072
0.311CysAsn: 0.311 ± 0.116
0.311CysPro: 0.311 ± 0.132
0.259CysGln: 0.259 ± 0.111
0.466CysArg: 0.466 ± 0.189
0.622CysSer: 0.622 ± 0.216
0.363CysThr: 0.363 ± 0.21
0.363CysVal: 0.363 ± 0.139
0.363CysTrp: 0.363 ± 0.132
0.155CysTyr: 0.155 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
5.079AspAla: 5.079 ± 0.613
0.155AspCys: 0.155 ± 0.093
3.939AspAsp: 3.939 ± 0.662
5.027AspGlu: 5.027 ± 0.695
2.747AspPhe: 2.747 ± 0.425
4.716AspGly: 4.716 ± 0.492
1.14AspHis: 1.14 ± 0.234
3.732AspIle: 3.732 ± 0.546
3.421AspLys: 3.421 ± 0.625
4.665AspLeu: 4.665 ± 0.647
1.918AspMet: 1.918 ± 0.322
2.747AspAsn: 2.747 ± 0.389
3.421AspPro: 3.421 ± 0.53
2.436AspGln: 2.436 ± 0.357
3.369AspArg: 3.369 ± 0.401
3.369AspSer: 3.369 ± 0.408
3.162AspThr: 3.162 ± 0.402
3.732AspVal: 3.732 ± 0.473
0.985AspTrp: 0.985 ± 0.225
1.866AspTyr: 1.866 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
5.546GluAla: 5.546 ± 0.479
0.622GluCys: 0.622 ± 0.217
4.146GluAsp: 4.146 ± 0.578
5.183GluGlu: 5.183 ± 0.577
2.073GluPhe: 2.073 ± 0.387
4.976GluGly: 4.976 ± 0.589
1.192GluHis: 1.192 ± 0.285
4.406GluIle: 4.406 ± 0.447
4.716GluLys: 4.716 ± 0.555
6.79GluLeu: 6.79 ± 0.68
2.591GluMet: 2.591 ± 0.369
2.799GluAsn: 2.799 ± 0.408
2.073GluPro: 2.073 ± 0.39
2.954GluGln: 2.954 ± 0.366
3.006GluArg: 3.006 ± 0.486
4.043GluSer: 4.043 ± 0.42
2.902GluThr: 2.902 ± 0.401
4.768GluVal: 4.768 ± 0.454
1.451GluTrp: 1.451 ± 0.286
2.229GluTyr: 2.229 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.229PheAla: 2.229 ± 0.331
0.518PheCys: 0.518 ± 0.152
3.576PheAsp: 3.576 ± 0.43
3.11PheGlu: 3.11 ± 0.455
1.451PhePhe: 1.451 ± 0.279
2.643PheGly: 2.643 ± 0.42
0.415PheHis: 0.415 ± 0.147
1.762PheIle: 1.762 ± 0.275
1.97PheLys: 1.97 ± 0.331
2.851PheLeu: 2.851 ± 0.401
1.244PheMet: 1.244 ± 0.255
1.762PheAsn: 1.762 ± 0.292
1.555PhePro: 1.555 ± 0.293
0.777PheGln: 0.777 ± 0.22
1.814PheArg: 1.814 ± 0.257
1.918PheSer: 1.918 ± 0.381
2.125PheThr: 2.125 ± 0.38
1.97PheVal: 1.97 ± 0.343
0.363PheTrp: 0.363 ± 0.138
0.933PheTyr: 0.933 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
6.582GlyAla: 6.582 ± 1.002
0.466GlyCys: 0.466 ± 0.186
4.976GlyAsp: 4.976 ± 0.477
4.354GlyGlu: 4.354 ± 0.475
2.488GlyPhe: 2.488 ± 0.365
5.649GlyGly: 5.649 ± 0.701
1.244GlyHis: 1.244 ± 0.283
4.872GlyIle: 4.872 ± 1.035
4.043GlyLys: 4.043 ± 0.426
5.649GlyLeu: 5.649 ± 1.105
2.695GlyMet: 2.695 ± 0.427
2.851GlyAsn: 2.851 ± 0.436
2.902GlyPro: 2.902 ± 0.364
2.695GlyGln: 2.695 ± 0.471
3.213GlyArg: 3.213 ± 0.382
5.235GlySer: 5.235 ± 0.61
5.235GlyThr: 5.235 ± 0.472
6.323GlyVal: 6.323 ± 0.628
0.881GlyTrp: 0.881 ± 0.199
2.54GlyTyr: 2.54 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.399HisAla: 1.399 ± 0.361
0.104HisCys: 0.104 ± 0.068
1.037HisAsp: 1.037 ± 0.22
1.607HisGlu: 1.607 ± 0.318
0.622HisPhe: 0.622 ± 0.214
1.71HisGly: 1.71 ± 0.288
0.57HisHis: 0.57 ± 0.166
1.866HisIle: 1.866 ± 0.387
0.829HisLys: 0.829 ± 0.257
1.399HisLeu: 1.399 ± 0.286
0.466HisMet: 0.466 ± 0.167
0.829HisAsn: 0.829 ± 0.22
0.985HisPro: 0.985 ± 0.267
0.518HisGln: 0.518 ± 0.138
1.451HisArg: 1.451 ± 0.273
1.088HisSer: 1.088 ± 0.258
0.726HisThr: 0.726 ± 0.171
1.348HisVal: 1.348 ± 0.283
0.155HisTrp: 0.155 ± 0.09
1.14HisTyr: 1.14 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.442IleAla: 5.442 ± 0.524
0.57IleCys: 0.57 ± 0.21
4.198IleAsp: 4.198 ± 0.413
4.25IleGlu: 4.25 ± 0.575
2.125IlePhe: 2.125 ± 0.298
4.716IleGly: 4.716 ± 0.792
1.296IleHis: 1.296 ± 0.297
2.695IleIle: 2.695 ± 0.496
3.576IleLys: 3.576 ± 0.407
4.665IleLeu: 4.665 ± 0.603
0.933IleMet: 0.933 ± 0.206
2.073IleAsn: 2.073 ± 0.323
2.436IlePro: 2.436 ± 0.293
1.866IleGln: 1.866 ± 0.34
2.954IleArg: 2.954 ± 0.332
3.11IleSer: 3.11 ± 0.448
3.524IleThr: 3.524 ± 0.471
4.768IleVal: 4.768 ± 0.479
0.674IleTrp: 0.674 ± 0.184
1.918IleTyr: 1.918 ± 0.389
0.0IleXaa: 0.0 ± 0.0
Lys
5.338LysAla: 5.338 ± 0.481
0.311LysCys: 0.311 ± 0.138
3.162LysAsp: 3.162 ± 0.579
4.354LysGlu: 4.354 ± 0.491
1.814LysPhe: 1.814 ± 0.438
4.354LysGly: 4.354 ± 0.55
1.71LysHis: 1.71 ± 0.339
3.11LysIle: 3.11 ± 0.465
3.524LysLys: 3.524 ± 0.656
4.976LysLeu: 4.976 ± 0.573
1.399LysMet: 1.399 ± 0.27
2.799LysAsn: 2.799 ± 0.327
3.058LysPro: 3.058 ± 0.512
2.073LysGln: 2.073 ± 0.262
2.488LysArg: 2.488 ± 0.394
3.835LysSer: 3.835 ± 0.489
3.317LysThr: 3.317 ± 0.351
4.976LysVal: 4.976 ± 0.551
1.037LysTrp: 1.037 ± 0.243
1.814LysTyr: 1.814 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
7.878LeuAla: 7.878 ± 1.358
0.518LeuCys: 0.518 ± 0.193
5.338LeuAsp: 5.338 ± 0.573
3.732LeuGlu: 3.732 ± 0.436
2.384LeuPhe: 2.384 ± 0.375
6.012LeuGly: 6.012 ± 1.009
1.244LeuHis: 1.244 ± 0.312
4.198LeuIle: 4.198 ± 0.572
5.649LeuLys: 5.649 ± 0.584
5.183LeuLeu: 5.183 ± 0.63
1.97LeuMet: 1.97 ± 0.35
4.509LeuAsn: 4.509 ± 0.496
2.488LeuPro: 2.488 ± 0.369
2.229LeuGln: 2.229 ± 0.336
3.835LeuArg: 3.835 ± 0.47
6.168LeuSer: 6.168 ± 0.767
6.79LeuThr: 6.79 ± 0.642
6.634LeuVal: 6.634 ± 0.516
1.037LeuTrp: 1.037 ± 0.229
2.851LeuTyr: 2.851 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
2.643MetAla: 2.643 ± 0.491
0.207MetCys: 0.207 ± 0.11
1.451MetAsp: 1.451 ± 0.273
1.659MetGlu: 1.659 ± 0.361
0.674MetPhe: 0.674 ± 0.166
1.814MetGly: 1.814 ± 0.325
0.57MetHis: 0.57 ± 0.161
1.918MetIle: 1.918 ± 0.271
1.607MetLys: 1.607 ± 0.266
2.54MetLeu: 2.54 ± 0.362
1.037MetMet: 1.037 ± 0.296
1.088MetAsn: 1.088 ± 0.223
1.296MetPro: 1.296 ± 0.295
0.881MetGln: 0.881 ± 0.191
1.348MetArg: 1.348 ± 0.287
2.281MetSer: 2.281 ± 0.37
2.54MetThr: 2.54 ± 0.36
1.762MetVal: 1.762 ± 0.299
0.259MetTrp: 0.259 ± 0.132
0.466MetTyr: 0.466 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.421AsnAla: 3.421 ± 0.459
0.311AsnCys: 0.311 ± 0.151
3.213AsnAsp: 3.213 ± 0.505
3.317AsnGlu: 3.317 ± 0.394
1.555AsnPhe: 1.555 ± 0.256
3.058AsnGly: 3.058 ± 0.515
1.244AsnHis: 1.244 ± 0.283
1.866AsnIle: 1.866 ± 0.263
2.281AsnLys: 2.281 ± 0.39
3.835AsnLeu: 3.835 ± 0.424
0.985AsnMet: 0.985 ± 0.304
1.71AsnAsn: 1.71 ± 0.31
2.747AsnPro: 2.747 ± 0.4
1.503AsnGln: 1.503 ± 0.244
3.006AsnArg: 3.006 ± 0.485
2.799AsnSer: 2.799 ± 0.39
2.747AsnThr: 2.747 ± 0.324
2.281AsnVal: 2.281 ± 0.385
0.363AsnTrp: 0.363 ± 0.147
1.296AsnTyr: 1.296 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
3.265ProAla: 3.265 ± 0.456
0.466ProCys: 0.466 ± 0.187
2.643ProAsp: 2.643 ± 0.44
4.095ProGlu: 4.095 ± 0.458
1.814ProPhe: 1.814 ± 0.313
2.799ProGly: 2.799 ± 0.414
0.881ProHis: 0.881 ± 0.261
1.814ProIle: 1.814 ± 0.294
2.332ProLys: 2.332 ± 0.368
2.384ProLeu: 2.384 ± 0.396
1.296ProMet: 1.296 ± 0.238
2.177ProAsn: 2.177 ± 0.349
2.073ProPro: 2.073 ± 0.49
1.244ProGln: 1.244 ± 0.242
1.97ProArg: 1.97 ± 0.346
3.058ProSer: 3.058 ± 0.515
2.902ProThr: 2.902 ± 0.569
3.11ProVal: 3.11 ± 0.403
0.57ProTrp: 0.57 ± 0.129
1.555ProTyr: 1.555 ± 0.288
0.0ProXaa: 0.0 ± 0.0
Gln
3.576GlnAla: 3.576 ± 0.581
0.155GlnCys: 0.155 ± 0.093
1.451GlnAsp: 1.451 ± 0.281
1.71GlnGlu: 1.71 ± 0.298
1.037GlnPhe: 1.037 ± 0.247
2.54GlnGly: 2.54 ± 0.419
1.192GlnHis: 1.192 ± 0.296
1.814GlnIle: 1.814 ± 0.324
1.659GlnLys: 1.659 ± 0.348
3.835GlnLeu: 3.835 ± 0.364
1.037GlnMet: 1.037 ± 0.239
1.503GlnAsn: 1.503 ± 0.292
1.555GlnPro: 1.555 ± 0.334
1.866GlnGln: 1.866 ± 0.354
2.021GlnArg: 2.021 ± 0.303
1.97GlnSer: 1.97 ± 0.328
2.229GlnThr: 2.229 ± 0.358
2.747GlnVal: 2.747 ± 0.367
0.363GlnTrp: 0.363 ± 0.182
1.037GlnTyr: 1.037 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
4.095ArgAla: 4.095 ± 0.41
0.363ArgCys: 0.363 ± 0.123
2.488ArgAsp: 2.488 ± 0.323
3.317ArgGlu: 3.317 ± 0.451
1.762ArgPhe: 1.762 ± 0.348
2.902ArgGly: 2.902 ± 0.443
1.088ArgHis: 1.088 ± 0.272
2.643ArgIle: 2.643 ± 0.313
3.524ArgLys: 3.524 ± 0.6
4.716ArgLeu: 4.716 ± 0.585
1.348ArgMet: 1.348 ± 0.273
2.229ArgAsn: 2.229 ± 0.382
2.799ArgPro: 2.799 ± 0.438
2.591ArgGln: 2.591 ± 0.438
3.006ArgArg: 3.006 ± 0.538
2.591ArgSer: 2.591 ± 0.437
3.265ArgThr: 3.265 ± 0.436
3.369ArgVal: 3.369 ± 0.369
0.622ArgTrp: 0.622 ± 0.219
1.451ArgTyr: 1.451 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
4.457SerAla: 4.457 ± 0.54
0.259SerCys: 0.259 ± 0.155
3.835SerAsp: 3.835 ± 0.454
5.39SerGlu: 5.39 ± 0.521
1.97SerPhe: 1.97 ± 0.348
5.338SerGly: 5.338 ± 0.573
1.192SerHis: 1.192 ± 0.26
3.68SerIle: 3.68 ± 0.323
3.887SerLys: 3.887 ± 0.619
5.079SerLeu: 5.079 ± 0.532
1.814SerMet: 1.814 ± 0.283
3.213SerAsn: 3.213 ± 0.455
2.436SerPro: 2.436 ± 0.329
2.229SerGln: 2.229 ± 0.323
2.332SerArg: 2.332 ± 0.33
4.509SerSer: 4.509 ± 0.613
4.82SerThr: 4.82 ± 0.504
4.095SerVal: 4.095 ± 0.461
1.296SerTrp: 1.296 ± 0.329
2.54SerTyr: 2.54 ± 0.396
0.0SerXaa: 0.0 ± 0.0
Thr
6.116ThrAla: 6.116 ± 0.786
0.363ThrCys: 0.363 ± 0.15
3.68ThrAsp: 3.68 ± 0.44
3.524ThrGlu: 3.524 ± 0.405
2.332ThrPhe: 2.332 ± 0.34
5.546ThrGly: 5.546 ± 0.498
0.881ThrHis: 0.881 ± 0.288
4.613ThrIle: 4.613 ± 0.61
3.628ThrLys: 3.628 ± 0.511
5.235ThrLeu: 5.235 ± 0.496
1.71ThrMet: 1.71 ± 0.293
2.488ThrAsn: 2.488 ± 0.375
3.473ThrPro: 3.473 ± 0.526
1.866ThrGln: 1.866 ± 0.323
2.643ThrArg: 2.643 ± 0.356
4.665ThrSer: 4.665 ± 0.376
4.768ThrThr: 4.768 ± 0.586
5.027ThrVal: 5.027 ± 0.639
0.57ThrTrp: 0.57 ± 0.209
2.125ThrTyr: 2.125 ± 0.467
0.0ThrXaa: 0.0 ± 0.0
Val
6.79ValAla: 6.79 ± 0.586
0.415ValCys: 0.415 ± 0.146
4.406ValAsp: 4.406 ± 0.364
4.924ValGlu: 4.924 ± 0.506
2.436ValPhe: 2.436 ± 0.339
5.287ValGly: 5.287 ± 0.626
1.399ValHis: 1.399 ± 0.337
4.509ValIle: 4.509 ± 0.636
3.991ValLys: 3.991 ± 0.527
5.494ValLeu: 5.494 ± 0.514
1.762ValMet: 1.762 ± 0.291
2.488ValAsn: 2.488 ± 0.356
3.317ValPro: 3.317 ± 0.433
2.591ValGln: 2.591 ± 0.421
3.939ValArg: 3.939 ± 0.565
5.079ValSer: 5.079 ± 0.507
5.494ValThr: 5.494 ± 0.678
6.064ValVal: 6.064 ± 0.68
1.037ValTrp: 1.037 ± 0.242
2.591ValTyr: 2.591 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.933TrpAla: 0.933 ± 0.179
0.155TrpCys: 0.155 ± 0.103
0.777TrpAsp: 0.777 ± 0.235
1.088TrpGlu: 1.088 ± 0.213
0.57TrpPhe: 0.57 ± 0.207
0.933TrpGly: 0.933 ± 0.2
0.363TrpHis: 0.363 ± 0.146
0.57TrpIle: 0.57 ± 0.154
0.622TrpLys: 0.622 ± 0.151
1.14TrpLeu: 1.14 ± 0.296
0.207TrpMet: 0.207 ± 0.12
1.14TrpAsn: 1.14 ± 0.258
0.674TrpPro: 0.674 ± 0.246
0.259TrpGln: 0.259 ± 0.104
0.777TrpArg: 0.777 ± 0.199
0.777TrpSer: 0.777 ± 0.204
0.985TrpThr: 0.985 ± 0.226
0.829TrpVal: 0.829 ± 0.197
0.104TrpTrp: 0.104 ± 0.069
0.466TrpTyr: 0.466 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.006TyrAla: 3.006 ± 0.611
0.259TyrCys: 0.259 ± 0.121
1.866TyrAsp: 1.866 ± 0.378
1.555TyrGlu: 1.555 ± 0.377
1.348TyrPhe: 1.348 ± 0.288
2.902TyrGly: 2.902 ± 0.438
0.57TyrHis: 0.57 ± 0.166
1.918TyrIle: 1.918 ± 0.369
2.125TyrLys: 2.125 ± 0.458
2.591TyrLeu: 2.591 ± 0.338
0.466TyrMet: 0.466 ± 0.179
1.244TyrAsn: 1.244 ± 0.229
1.348TyrPro: 1.348 ± 0.242
1.14TyrGln: 1.14 ± 0.245
1.866TyrArg: 1.866 ± 0.301
2.332TyrSer: 2.332 ± 0.37
1.866TyrThr: 1.866 ± 0.34
2.384TyrVal: 2.384 ± 0.272
0.518TyrTrp: 0.518 ± 0.169
0.674TyrTyr: 0.674 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (19295 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski