Escherichia phage K1F (Bacteriophage K1F)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus K1F

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3YJY1|Q3YJY1_BPK1F Isoform of Q3YJY2 Uncharacterized protein 5.0 OS=Escherichia phage K1F OX=344021 GN=5.0 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.26LFNEE21 pKa = 5.17RR22 pKa = 11.84IQYY25 pKa = 10.64DD26 pKa = 4.52EE27 pKa = 3.95ITEE30 pKa = 4.26GDD32 pKa = 4.1DD33 pKa = 3.71YY34 pKa = 11.83SDD36 pKa = 4.03ALHH39 pKa = 6.32EE40 pKa = 4.48VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.81IFTVMAADD60 pKa = 5.4GIDD63 pKa = 3.84HH64 pKa = 6.56EE65 pKa = 5.23FEE67 pKa = 5.3DD68 pKa = 4.36SGLMPEE74 pKa = 4.55TKK76 pKa = 10.2DD77 pKa = 3.18VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.8NDD93 pKa = 3.77VSNSSEE99 pKa = 4.36VVWFEE104 pKa = 5.02AEE106 pKa = 4.13EE107 pKa = 3.99EE108 pKa = 4.22DD109 pKa = 4.58EE110 pKa = 4.33KK111 pKa = 11.69AEE113 pKa = 4.0YY114 pKa = 9.47WVVDD118 pKa = 3.43TRR120 pKa = 11.84TGEE123 pKa = 4.05IIEE126 pKa = 4.52AAVSLYY132 pKa = 10.57EE133 pKa = 5.01AIACAKK139 pKa = 9.51EE140 pKa = 3.86LYY142 pKa = 10.45EE143 pKa = 4.8EE144 pKa = 4.81GFHH147 pKa = 6.14MQVEE151 pKa = 4.85DD152 pKa = 3.59INDD155 pKa = 3.4NVVFDD160 pKa = 4.29PAAAEE165 pKa = 4.13EE166 pKa = 4.35DD167 pKa = 4.35CEE169 pKa = 4.21

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3YJZ9|Q3YJZ9_BPK1F Gp0.36 protein OS=Escherichia phage K1F OX=344021 GN=0.36 PE=4 SV=1
MM1 pKa = 7.5SLALLIAIGYY11 pKa = 8.61GLIAYY16 pKa = 8.1VLVRR20 pKa = 11.84DD21 pKa = 3.27IDD23 pKa = 3.79KK24 pKa = 10.87ARR26 pKa = 11.84KK27 pKa = 8.48VYY29 pKa = 10.05KK30 pKa = 10.33FNYY33 pKa = 8.37VRR35 pKa = 11.84LGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 3.9IATIGSKK64 pKa = 10.35LL65 pKa = 3.44

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

1

43

11586

32

1295

269.4

30.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.692 ± 0.512

0.898 ± 0.174

6.516 ± 0.326

6.939 ± 0.413

3.634 ± 0.194

7.846 ± 0.366

1.726 ± 0.217

4.989 ± 0.2

6.283 ± 0.455

7.725 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.178

4.385 ± 0.271

3.962 ± 0.197

3.789 ± 0.408

5.541 ± 0.203

6.223 ± 0.365

5.835 ± 0.253

7.095 ± 0.314

1.493 ± 0.156

3.582 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski