Colletotrichum gloeosporioides (strain Cg-14) (Anthracnose fungus) (Glomerella cingulata)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum; Colletotrichum gloeosporioides species complex; Colletotrichum gloeosporioides

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0KNM6|T0KNM6_COLGC Protein kinase OS=Colletotrichum gloeosporioides (strain Cg-14) OX=1237896 GN=CGLO_06083 PE=4 SV=1
MM1 pKa = 7.57ANVPVTIDD9 pKa = 3.13MDD11 pKa = 4.21LGSNCLQDD19 pKa = 3.73PSDD22 pKa = 4.01YY23 pKa = 10.73STDD26 pKa = 3.85DD27 pKa = 3.24LTSYY31 pKa = 9.43YY32 pKa = 10.72AAYY35 pKa = 7.98RR36 pKa = 11.84TTSLTDD42 pKa = 3.15SFGSDD47 pKa = 2.93FSTYY51 pKa = 9.16QDD53 pKa = 3.4YY54 pKa = 11.56LDD56 pKa = 4.07YY57 pKa = 10.64YY58 pKa = 11.14KK59 pKa = 10.65KK60 pKa = 9.39VTQIGDD66 pKa = 3.18IGSALANMDD75 pKa = 3.51GQHH78 pKa = 6.28TLDD81 pKa = 4.46AYY83 pKa = 11.38NPDD86 pKa = 3.55EE87 pKa = 4.38SLTPSFLTFQTEE99 pKa = 3.63IDD101 pKa = 3.46GGLFDD106 pKa = 4.7GCIGSHH112 pKa = 5.37SSKK115 pKa = 9.73TFSLDD120 pKa = 2.92VDD122 pKa = 3.74GTSYY126 pKa = 11.03QITLEE131 pKa = 4.66DD132 pKa = 4.6PPTSQWTITQDD143 pKa = 3.81SPGDD147 pKa = 3.56PNRR150 pKa = 11.84IVIGSGGRR158 pKa = 11.84ANPLLTVLYY167 pKa = 9.96AVLGVEE173 pKa = 4.4ILALAGAVLGSALGGLAPAAVEE195 pKa = 4.07GTIAEE200 pKa = 4.37MGTSEE205 pKa = 4.06MTQIIEE211 pKa = 4.8DD212 pKa = 4.26KK213 pKa = 10.48LASDD217 pKa = 4.22PWVMQWMEE225 pKa = 3.72KK226 pKa = 10.05TIFGEE231 pKa = 4.3AEE233 pKa = 3.44AAAKK237 pKa = 10.05KK238 pKa = 9.84RR239 pKa = 11.84ASPVARR245 pKa = 11.84RR246 pKa = 11.84GVGAGGILQEE256 pKa = 3.83WTAAYY261 pKa = 7.59DD262 pKa = 3.12TWNYY266 pKa = 10.52DD267 pKa = 3.64DD268 pKa = 6.03RR269 pKa = 11.84DD270 pKa = 3.87ACVMSKK276 pKa = 10.91DD277 pKa = 3.87VIGEE281 pKa = 4.17LDD283 pKa = 3.95CLVVGTSIVIQADD296 pKa = 4.2GQPMAMPLPPLGSAWW311 pKa = 3.32

Molecular weight: 33.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0LYN2|T0LYN2_COLGC Uncharacterized protein OS=Colletotrichum gloeosporioides (strain Cg-14) OX=1237896 GN=CGLO_03306 PE=4 SV=1
MM1 pKa = 7.85PLRR4 pKa = 11.84STRR7 pKa = 11.84HH8 pKa = 4.05TTTTAPRR15 pKa = 11.84RR16 pKa = 11.84GGLFTRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84APARR28 pKa = 11.84THH30 pKa = 5.82HH31 pKa = 6.33HH32 pKa = 6.15HH33 pKa = 6.18TTTTTTTRR41 pKa = 11.84KK42 pKa = 9.94RR43 pKa = 11.84GGLFSRR49 pKa = 11.84RR50 pKa = 11.84HH51 pKa = 5.06GAVTTAPVHH60 pKa = 4.81HH61 pKa = 6.22QRR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 9.81PSMGDD70 pKa = 3.3KK71 pKa = 10.53ISGAMLKK78 pKa = 10.72LKK80 pKa = 9.36GTLTRR85 pKa = 11.84RR86 pKa = 11.84PGQKK90 pKa = 9.9AAGTRR95 pKa = 11.84RR96 pKa = 11.84MHH98 pKa = 5.68GTDD101 pKa = 2.6GRR103 pKa = 11.84GSHH106 pKa = 5.87RR107 pKa = 11.84RR108 pKa = 11.84YY109 pKa = 10.21RR110 pKa = 11.84YY111 pKa = 9.72

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16388

0

16388

6823665

8

6253

416.4

46.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.043 ± 0.017

1.253 ± 0.008

5.82 ± 0.014

6.055 ± 0.02

3.913 ± 0.012

7.144 ± 0.02

2.288 ± 0.009

4.899 ± 0.014

4.847 ± 0.02

8.725 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.007

3.728 ± 0.011

5.903 ± 0.022

3.797 ± 0.014

5.775 ± 0.017

7.731 ± 0.021

5.963 ± 0.018

6.371 ± 0.015

1.627 ± 0.008

2.815 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski