Pseudoloma neurophilia

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Pseudoloma

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R0LR62|A0A0R0LR62_9MICR Proteasome subunit alpha type (Fragment) OS=Pseudoloma neurophilia OX=146866 GN=M153_15306000744 PE=3 SV=1
VV1 pKa = 5.93FHH3 pKa = 7.51LYY5 pKa = 10.59LQFDD9 pKa = 4.61LDD11 pKa = 3.96VFHH14 pKa = 7.46LFLQFDD20 pKa = 4.75LDD22 pKa = 3.95VFDD25 pKa = 5.18LFLRR29 pKa = 11.84FDD31 pKa = 4.62LNVFDD36 pKa = 5.5LFLRR40 pKa = 11.84FDD42 pKa = 4.86LDD44 pKa = 3.56VFDD47 pKa = 4.78PLLRR51 pKa = 11.84IDD53 pKa = 4.88LDD55 pKa = 4.17VIHH58 pKa = 7.35LYY60 pKa = 10.72LQFDD64 pKa = 4.51LDD66 pKa = 3.93VFHH69 pKa = 7.65LYY71 pKa = 10.65LQFDD75 pKa = 4.61LDD77 pKa = 3.96VFHH80 pKa = 7.46LFLQFDD86 pKa = 4.5LDD88 pKa = 3.92VFHH91 pKa = 7.65LYY93 pKa = 10.67LQFDD97 pKa = 4.59LNVFHH102 pKa = 7.7LYY104 pKa = 10.61LQFDD108 pKa = 4.59LNVFHH113 pKa = 7.7LYY115 pKa = 10.59LQFDD119 pKa = 4.61LDD121 pKa = 3.96VFHH124 pKa = 7.46LFLQFDD130 pKa = 4.5LDD132 pKa = 3.92VFHH135 pKa = 7.65LYY137 pKa = 10.65LQFDD141 pKa = 4.75LDD143 pKa = 3.96VFDD146 pKa = 5.18LFLRR150 pKa = 11.84FDD152 pKa = 4.62LNVFDD157 pKa = 5.5LFLRR161 pKa = 11.84FDD163 pKa = 4.62LNVFDD168 pKa = 5.5LFLRR172 pKa = 11.84FDD174 pKa = 4.86LDD176 pKa = 3.56VFDD179 pKa = 4.78PLLRR183 pKa = 11.84IDD185 pKa = 4.88LDD187 pKa = 4.17VIHH190 pKa = 7.35LYY192 pKa = 10.72LQFDD196 pKa = 4.51LDD198 pKa = 3.93VFHH201 pKa = 7.65LYY203 pKa = 10.72LQFDD207 pKa = 4.49LDD209 pKa = 3.71

Molecular weight: 26.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R0LQQ8|A0A0R0LQQ8_9MICR Uncharacterized protein (Fragment) OS=Pseudoloma neurophilia OX=146866 GN=M153_2439900054 PE=4 SV=1
LL1 pKa = 6.5KK2 pKa = 9.25TQLVKK7 pKa = 10.71KK8 pKa = 9.41RR9 pKa = 11.84TVLKK13 pKa = 8.46VTPVKK18 pKa = 10.68QITPVKK24 pKa = 9.96KK25 pKa = 8.69VTVKK29 pKa = 10.3KK30 pKa = 9.51RR31 pKa = 11.84TVLKK35 pKa = 9.43VTSAKK40 pKa = 10.05KK41 pKa = 9.04RR42 pKa = 11.84TVLKK46 pKa = 8.69VTSVKK51 pKa = 10.22VRR53 pKa = 11.84VKK55 pKa = 10.46KK56 pKa = 9.41RR57 pKa = 11.84TPAKK61 pKa = 8.63VTVKK65 pKa = 10.23KK66 pKa = 9.69RR67 pKa = 11.84TVLKK71 pKa = 8.55VTPAKK76 pKa = 10.51KK77 pKa = 8.39ITSVKK82 pKa = 10.18KK83 pKa = 10.32IPNNTAPVKK92 pKa = 9.95KK93 pKa = 6.91TTPNNTTPVIKK104 pKa = 10.33HH105 pKa = 6.28LLMM108 pKa = 5.27

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3636

0

3636

957747

40

4828

263.4

30.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.325 ± 0.043

1.667 ± 0.022

5.699 ± 0.037

7.189 ± 0.05

5.804 ± 0.054

3.737 ± 0.047

2.107 ± 0.022

8.763 ± 0.047

9.96 ± 0.058

9.359 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.019

7.27 ± 0.052

3.054 ± 0.037

4.082 ± 0.037

3.976 ± 0.032

7.59 ± 0.048

5.457 ± 0.039

4.602 ± 0.031

0.597 ± 0.012

3.566 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski