Fiddler Crab associated circular virus
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLN8|A0A0K1RLN8_9CIRC Putative capsid protein OS=Fiddler Crab associated circular virus OX=1692249 PE=4 SV=1
MM1 pKa = 7.31 GVKK4 pKa = 10.47 LKK6 pKa = 10.62 QSQDD10 pKa = 3.01 NSEE13 pKa = 4.46 GGNNHH18 pKa = 6.47 PPSFRR23 pKa = 11.84 SRR25 pKa = 11.84 KK26 pKa = 9.64 LVFTLNNYY34 pKa = 8.41 QKK36 pKa = 11.16 EE37 pKa = 4.38 DD38 pKa = 3.19 INLIISYY45 pKa = 10.13 FEE47 pKa = 4.02 SSAQKK52 pKa = 9.17 WVFGEE57 pKa = 4.19 EE58 pKa = 4.22 VGEE61 pKa = 4.28 SGTPHH66 pKa = 5.89 LQGYY70 pKa = 9.1 CEE72 pKa = 4.38 FKK74 pKa = 10.74 NQKK77 pKa = 7.26 EE78 pKa = 4.35 WKK80 pKa = 9.92 QITDD84 pKa = 2.81 KK85 pKa = 11.17 CSPLKK90 pKa = 10.52 RR91 pKa = 11.84 AWSTSARR98 pKa = 11.84 GSLDD102 pKa = 4.47 DD103 pKa = 3.79 NVKK106 pKa = 9.22 YY107 pKa = 6.46 TTKK110 pKa = 10.54 DD111 pKa = 2.87 GKK113 pKa = 10.33 IYY115 pKa = 10.67 YY116 pKa = 10.36 GGFKK120 pKa = 9.68 IEE122 pKa = 3.78 KK123 pKa = 8.96 CNYY126 pKa = 8.83 KK127 pKa = 10.19 VEE129 pKa = 4.07 IDD131 pKa = 3.51 LYY133 pKa = 10.14 DD134 pKa = 3.39 WQKK137 pKa = 11.38 EE138 pKa = 3.75 IMEE141 pKa = 4.66 ILEE144 pKa = 3.99 QTPDD148 pKa = 3.3 DD149 pKa = 4.13 RR150 pKa = 11.84 TIHH153 pKa = 5.7 WVWEE157 pKa = 4.09 PKK159 pKa = 10.7 GGIGKK164 pKa = 7.01 TTFQKK169 pKa = 11.15 YY170 pKa = 9.24 IFTHH174 pKa = 5.08 MGGVCVLSGKK184 pKa = 10.37 GSDD187 pKa = 3.33 MKK189 pKa = 10.8 HH190 pKa = 5.3 GVVSYY195 pKa = 10.96 INDD198 pKa = 3.55 HH199 pKa = 6.6 GEE201 pKa = 3.92 TPDD204 pKa = 3.25 IVLINIPRR212 pKa = 11.84 SSAEE216 pKa = 3.8 FVSWSGIEE224 pKa = 4.16 EE225 pKa = 3.94 IKK227 pKa = 11.33 DD228 pKa = 3.06 MFFYY232 pKa = 10.48 SGKK235 pKa = 9.79 YY236 pKa = 9.55 EE237 pKa = 4.23 GGQVCGMSPHH247 pKa = 6.07 VLVFANTEE255 pKa = 3.81 PPEE258 pKa = 4.34 GVFSKK263 pKa = 10.99 DD264 pKa = 2.88 RR265 pKa = 11.84 VKK267 pKa = 10.26 TIEE270 pKa = 4.26 LDD272 pKa = 3.54 SS273 pKa = 4.04
Molecular weight: 31.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.594
IPC2_protein 5.626
IPC_protein 5.626
Toseland 5.944
ProMoST 5.919
Dawson 5.83
Bjellqvist 5.855
Wikipedia 5.804
Rodwell 5.804
Grimsley 6.059
Solomon 5.83
Lehninger 5.817
Nozaki 6.059
DTASelect 6.237
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.16
Patrickios 4.088
IPC_peptide 5.842
IPC2_peptide 6.135
IPC2.peptide.svr19 6.204
Protein with the highest isoelectric point:
>tr|A0A0K1RLN8|A0A0K1RLN8_9CIRC Putative capsid protein OS=Fiddler Crab associated circular virus OX=1692249 PE=4 SV=1
MM1 pKa = 7.82 PYY3 pKa = 10.6 ARR5 pKa = 11.84 KK6 pKa = 6.87 TQKK9 pKa = 9.82 SRR11 pKa = 11.84 KK12 pKa = 6.16 RR13 pKa = 11.84 TYY15 pKa = 9.66 RR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 8.02 TYY20 pKa = 9.82 RR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 10.47 NLVLSKK29 pKa = 11.11 APIPNTFSTKK39 pKa = 9.66 LRR41 pKa = 11.84 YY42 pKa = 9.4 SEE44 pKa = 4.38 SVSVNPGASGVAGVYY59 pKa = 10.35 VMNASSCYY67 pKa = 10.32 DD68 pKa = 3.57 PNTTGTGHH76 pKa = 5.7 QPRR79 pKa = 11.84 GFDD82 pKa = 2.85 QWMTMYY88 pKa = 11.26 DD89 pKa = 3.49 HH90 pKa = 6.49 FTVIGAKK97 pKa = 8.42 ITAQFSITGQADD109 pKa = 3.4 DD110 pKa = 4.24 RR111 pKa = 11.84 ALICGIALKK120 pKa = 10.35 DD121 pKa = 3.74 ANGVYY126 pKa = 10.35 ADD128 pKa = 4.26 KK129 pKa = 11.02 NDD131 pKa = 3.59 YY132 pKa = 10.07 MEE134 pKa = 4.28 GRR136 pKa = 11.84 NVVSKK141 pKa = 10.43 LVSGGATGDD150 pKa = 3.38 ARR152 pKa = 11.84 TVTLSKK158 pKa = 9.79 TYY160 pKa = 9.85 SAKK163 pKa = 10.5 KK164 pKa = 9.71 FLGISKK170 pKa = 9.52 PLASSLIRR178 pKa = 11.84 GTSSANPSEE187 pKa = 3.85 NAYY190 pKa = 10.14 FHH192 pKa = 6.89 LFAAPTGNADD202 pKa = 4.34 LDD204 pKa = 4.32 NIPIQVLIDD213 pKa = 3.56 YY214 pKa = 9.22 LVVFTEE220 pKa = 4.53 PQQPSQSS227 pKa = 3.42
Molecular weight: 24.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.867
IPC2_protein 9.268
IPC_protein 9.238
Toseland 9.706
ProMoST 9.531
Dawson 9.999
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.277
Grimsley 10.101
Solomon 10.028
Lehninger 9.984
Nozaki 9.736
DTASelect 9.706
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.911
Patrickios 5.08
IPC_peptide 10.014
IPC2_peptide 8.39
IPC2.peptide.svr19 8.17
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
500
227
273
250.0
27.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.2 ± 2.598
1.4 ± 0.333
5.6 ± 0.201
5.2 ± 2.205
4.2 ± 0.433
8.6 ± 0.712
2.0 ± 0.435
6.0 ± 0.74
8.2 ± 1.021
5.4 ± 0.492
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.002
4.8 ± 0.312
4.6 ± 0.44
4.0 ± 0.023
4.0 ± 1.107
9.0 ± 0.444
6.6 ± 1.135
6.6 ± 0.005
1.6 ± 0.744
4.8 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here