Fiddler Crab associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0K1RLN8|A0A0K1RLN8_9CIRC Putative capsid protein OS=Fiddler Crab associated circular virus OX=1692249 PE=4 SV=1
MM1 pKa = 7.31GVKK4 pKa = 10.47LKK6 pKa = 10.62QSQDD10 pKa = 3.01NSEE13 pKa = 4.46GGNNHH18 pKa = 6.47PPSFRR23 pKa = 11.84SRR25 pKa = 11.84KK26 pKa = 9.64LVFTLNNYY34 pKa = 8.41QKK36 pKa = 11.16EE37 pKa = 4.38DD38 pKa = 3.19INLIISYY45 pKa = 10.13FEE47 pKa = 4.02SSAQKK52 pKa = 9.17WVFGEE57 pKa = 4.19EE58 pKa = 4.22VGEE61 pKa = 4.28SGTPHH66 pKa = 5.89LQGYY70 pKa = 9.1CEE72 pKa = 4.38FKK74 pKa = 10.74NQKK77 pKa = 7.26EE78 pKa = 4.35WKK80 pKa = 9.92QITDD84 pKa = 2.81KK85 pKa = 11.17CSPLKK90 pKa = 10.52RR91 pKa = 11.84AWSTSARR98 pKa = 11.84GSLDD102 pKa = 4.47DD103 pKa = 3.79NVKK106 pKa = 9.22YY107 pKa = 6.46TTKK110 pKa = 10.54DD111 pKa = 2.87GKK113 pKa = 10.33IYY115 pKa = 10.67YY116 pKa = 10.36GGFKK120 pKa = 9.68IEE122 pKa = 3.78KK123 pKa = 8.96CNYY126 pKa = 8.83KK127 pKa = 10.19VEE129 pKa = 4.07IDD131 pKa = 3.51LYY133 pKa = 10.14DD134 pKa = 3.39WQKK137 pKa = 11.38EE138 pKa = 3.75IMEE141 pKa = 4.66ILEE144 pKa = 3.99QTPDD148 pKa = 3.3DD149 pKa = 4.13RR150 pKa = 11.84TIHH153 pKa = 5.7WVWEE157 pKa = 4.09PKK159 pKa = 10.7GGIGKK164 pKa = 7.01TTFQKK169 pKa = 11.15YY170 pKa = 9.24IFTHH174 pKa = 5.08MGGVCVLSGKK184 pKa = 10.37GSDD187 pKa = 3.33MKK189 pKa = 10.8HH190 pKa = 5.3GVVSYY195 pKa = 10.96INDD198 pKa = 3.55HH199 pKa = 6.6GEE201 pKa = 3.92TPDD204 pKa = 3.25IVLINIPRR212 pKa = 11.84SSAEE216 pKa = 3.8FVSWSGIEE224 pKa = 4.16EE225 pKa = 3.94IKK227 pKa = 11.33DD228 pKa = 3.06MFFYY232 pKa = 10.48SGKK235 pKa = 9.79YY236 pKa = 9.55EE237 pKa = 4.23GGQVCGMSPHH247 pKa = 6.07VLVFANTEE255 pKa = 3.81PPEE258 pKa = 4.34GVFSKK263 pKa = 10.99DD264 pKa = 2.88RR265 pKa = 11.84VKK267 pKa = 10.26TIEE270 pKa = 4.26LDD272 pKa = 3.54SS273 pKa = 4.04

Molecular weight:
31.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0K1RLN8|A0A0K1RLN8_9CIRC Putative capsid protein OS=Fiddler Crab associated circular virus OX=1692249 PE=4 SV=1
MM1 pKa = 7.82PYY3 pKa = 10.6ARR5 pKa = 11.84KK6 pKa = 6.87TQKK9 pKa = 9.82SRR11 pKa = 11.84KK12 pKa = 6.16RR13 pKa = 11.84TYY15 pKa = 9.66RR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 8.02TYY20 pKa = 9.82RR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.47NLVLSKK29 pKa = 11.11APIPNTFSTKK39 pKa = 9.66LRR41 pKa = 11.84YY42 pKa = 9.4SEE44 pKa = 4.38SVSVNPGASGVAGVYY59 pKa = 10.35VMNASSCYY67 pKa = 10.32DD68 pKa = 3.57PNTTGTGHH76 pKa = 5.7QPRR79 pKa = 11.84GFDD82 pKa = 2.85QWMTMYY88 pKa = 11.26DD89 pKa = 3.49HH90 pKa = 6.49FTVIGAKK97 pKa = 8.42ITAQFSITGQADD109 pKa = 3.4DD110 pKa = 4.24RR111 pKa = 11.84ALICGIALKK120 pKa = 10.35DD121 pKa = 3.74ANGVYY126 pKa = 10.35ADD128 pKa = 4.26KK129 pKa = 11.02NDD131 pKa = 3.59YY132 pKa = 10.07MEE134 pKa = 4.28GRR136 pKa = 11.84NVVSKK141 pKa = 10.43LVSGGATGDD150 pKa = 3.38ARR152 pKa = 11.84TVTLSKK158 pKa = 9.79TYY160 pKa = 9.85SAKK163 pKa = 10.5KK164 pKa = 9.71FLGISKK170 pKa = 9.52PLASSLIRR178 pKa = 11.84GTSSANPSEE187 pKa = 3.85NAYY190 pKa = 10.14FHH192 pKa = 6.89LFAAPTGNADD202 pKa = 4.34LDD204 pKa = 4.32NIPIQVLIDD213 pKa = 3.56YY214 pKa = 9.22LVVFTEE220 pKa = 4.53PQQPSQSS227 pKa = 3.42

Molecular weight:
24.77 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

500

227

273

250.0

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.2 ± 2.598

1.4 ± 0.333

5.6 ± 0.201

5.2 ± 2.205

4.2 ± 0.433

8.6 ± 0.712

2.0 ± 0.435

6.0 ± 0.74

8.2 ± 1.021

5.4 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.002

4.8 ± 0.312

4.6 ± 0.44

4.0 ± 0.023

4.0 ± 1.107

9.0 ± 0.444

6.6 ± 1.135

6.6 ± 0.005

1.6 ± 0.744

4.8 ± 0.312

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski