Neomicrococcus aestuarii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Neomicrococcus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2ZQR2|A0A1L2ZQR2_9MICC UDP-glucose 6-dehydrogenase OS=Neomicrococcus aestuarii OX=556325 GN=BHE16_11640 PE=3 SV=1
MM1 pKa = 7.33SKK3 pKa = 10.14VLSSLRR9 pKa = 11.84YY10 pKa = 8.4SAEE13 pKa = 4.01HH14 pKa = 6.13EE15 pKa = 4.15WLDD18 pKa = 3.26NSTPAKK24 pKa = 10.55VGISQVAADD33 pKa = 3.85ALGDD37 pKa = 3.73VVYY40 pKa = 11.27VDD42 pKa = 4.12LPEE45 pKa = 4.43VGSEE49 pKa = 3.93VTAGEE54 pKa = 4.19TCGEE58 pKa = 4.21VEE60 pKa = 4.36STKK63 pKa = 10.7SVSDD67 pKa = 4.02LYY69 pKa = 11.42SPVSGTVVEE78 pKa = 4.83VNQEE82 pKa = 4.38AIDD85 pKa = 3.85NPAILNEE92 pKa = 4.11DD93 pKa = 4.08PYY95 pKa = 10.62VAGWLFAVEE104 pKa = 4.28VSEE107 pKa = 4.72EE108 pKa = 4.4GPLLSAAEE116 pKa = 4.07YY117 pKa = 10.82ASANGGDD124 pKa = 3.79VEE126 pKa = 4.41

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2ZNR7|A0A1L2ZNR7_9MICC ABC transporter domain-containing protein OS=Neomicrococcus aestuarii OX=556325 GN=BHE16_07385 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.03GRR40 pKa = 11.84TALSAA45 pKa = 3.73

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2289

0

2289

763650

45

2147

333.6

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.737 ± 0.065

0.548 ± 0.012

5.629 ± 0.04

6.489 ± 0.054

3.478 ± 0.03

8.207 ± 0.042

2.09 ± 0.027

5.004 ± 0.037

3.326 ± 0.037

9.838 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.019

2.878 ± 0.024

4.891 ± 0.036

3.3 ± 0.027

6.163 ± 0.045

6.534 ± 0.039

5.913 ± 0.035

8.338 ± 0.041

1.402 ± 0.023

2.149 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski