Enterobacteria phage T6 (Bacteriophage T6)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus; Enterobacteria virus T6

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FJE2|A0A346FJE2_BPT6 Hemolysin OS=Enterobacteria phage T6 OX=10666 GN=XhlA PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.53FSTFDD7 pKa = 3.29INDD10 pKa = 3.39EE11 pKa = 4.56FIANIDD17 pKa = 3.55YY18 pKa = 10.34TEE20 pKa = 4.07EE21 pKa = 3.89DD22 pKa = 3.19SRR24 pKa = 11.84YY25 pKa = 10.52VGIIYY30 pKa = 8.39ITSNSAQGVVCMAEE44 pKa = 3.64FDD46 pKa = 4.62EE47 pKa = 5.29YY48 pKa = 11.33FLDD51 pKa = 4.14YY52 pKa = 11.25DD53 pKa = 6.38DD54 pKa = 4.72MIEE57 pKa = 3.75WSKK60 pKa = 11.35RR61 pKa = 11.84YY62 pKa = 9.6IKK64 pKa = 10.67RR65 pKa = 11.84NLLL68 pKa = 3.63

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FJZ4|A0A346FJZ4_BPT6 Uncharacterized protein OS=Enterobacteria phage T6 OX=10666 GN=EcT6_00265 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.71YY3 pKa = 7.67MTVTDD8 pKa = 4.89LNNAGATVIGTIKK21 pKa = 10.32GGEE24 pKa = 3.97WFLGTPHH31 pKa = 7.42KK32 pKa = 10.82DD33 pKa = 2.94ILSKK37 pKa = 10.58PGFYY41 pKa = 10.67FLVSKK46 pKa = 11.03LGGPFSSPCVSARR59 pKa = 11.84FYY61 pKa = 11.36VGNQRR66 pKa = 11.84SKK68 pKa = 10.58QGFSAVLSHH77 pKa = 6.11IRR79 pKa = 11.84QRR81 pKa = 11.84RR82 pKa = 11.84SQLARR87 pKa = 11.84TIANNNVPYY96 pKa = 10.21TVFYY100 pKa = 11.09LPASKK105 pKa = 9.84MKK107 pKa = 10.21PLTTGFGKK115 pKa = 10.64GQLALAFTRR124 pKa = 11.84NHH126 pKa = 5.74HH127 pKa = 6.17SEE129 pKa = 4.15YY130 pKa = 9.53QTLEE134 pKa = 3.57EE135 pKa = 4.44MNRR138 pKa = 11.84MLADD142 pKa = 3.15NFKK145 pKa = 10.83FVLQAYY151 pKa = 9.08

Molecular weight: 16.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

52553

28

1289

196.1

22.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.257 ± 0.173

1.142 ± 0.073

6.14 ± 0.124

7.284 ± 0.159

4.418 ± 0.121

5.924 ± 0.212

1.722 ± 0.084

7.608 ± 0.118

8.23 ± 0.234

7.404 ± 0.117

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.102

5.787 ± 0.126

3.416 ± 0.094

3.241 ± 0.105

4.278 ± 0.109

6.747 ± 0.159

5.585 ± 0.222

6.348 ± 0.112

1.408 ± 0.057

4.325 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski