Bremia lactucae (Lettuce downy mildew)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Bremia

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484E9N1|A0A484E9N1_BRELC DNA polymerase OS=Bremia lactucae OX=4779 GN=CCR75_005003 PE=3 SV=1
MM1 pKa = 7.47ALVASTFRR9 pKa = 11.84RR10 pKa = 11.84VVEE13 pKa = 4.43LPWNTLYY20 pKa = 10.71FLHH23 pKa = 7.53RR24 pKa = 11.84AMQWNVTPPILIEE37 pKa = 4.07VEE39 pKa = 4.36KK40 pKa = 10.55PGIWGYY46 pKa = 11.01DD47 pKa = 3.16LYY49 pKa = 10.69TPCSTKK55 pKa = 10.77DD56 pKa = 3.83DD57 pKa = 4.42YY58 pKa = 12.08DD59 pKa = 6.05DD60 pKa = 4.39DD61 pKa = 4.51TDD63 pKa = 4.22VSVSYY68 pKa = 11.24LNDD71 pKa = 3.66GSDD74 pKa = 4.27TEE76 pKa = 6.3DD77 pKa = 3.5EE78 pKa = 5.14DD79 pKa = 3.71EE80 pKa = 4.43WSCSGSEE87 pKa = 4.05AEE89 pKa = 4.29SDD91 pKa = 3.42GFAFGRR97 pKa = 11.84VDD99 pKa = 5.02NVEE102 pKa = 3.81LWGDD106 pKa = 3.28RR107 pKa = 11.84WFWTIDD113 pKa = 3.21HH114 pKa = 7.57DD115 pKa = 5.92DD116 pKa = 3.92DD117 pKa = 4.44TRR119 pKa = 11.84SCSDD123 pKa = 3.82LNDD126 pKa = 3.68NLDD129 pKa = 3.68INTAIYY135 pKa = 10.04IVEE138 pKa = 4.21KK139 pKa = 10.2PVDD142 pKa = 3.85YY143 pKa = 11.51VMPTMDD149 pKa = 3.69YY150 pKa = 11.19NDD152 pKa = 4.04YY153 pKa = 10.89SSEE156 pKa = 3.87AAIDD160 pKa = 3.25IWIEE164 pKa = 3.71KK165 pKa = 9.75YY166 pKa = 10.33GASRR170 pKa = 11.84CQVHH174 pKa = 6.38FVTVALDD181 pKa = 3.54EE182 pKa = 4.58PTSQDD187 pKa = 3.38AFQSTCGISQRR198 pKa = 11.84LFIAVLL204 pKa = 3.45

Molecular weight: 23.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484DVL2|A0A484DVL2_BRELC bPH_1 domain-containing protein OS=Bremia lactucae OX=4779 GN=CCR75_008816 PE=4 SV=1
MM1 pKa = 7.29TSSKK5 pKa = 11.16KK6 pKa = 10.59LMRR9 pKa = 11.84NYY11 pKa = 10.98DD12 pKa = 3.44VFGSMTLSCATISAATPPVVLCMSRR37 pKa = 11.84RR38 pKa = 11.84ILDD41 pKa = 3.58NKK43 pKa = 9.72KK44 pKa = 8.23GCAYY48 pKa = 9.56EE49 pKa = 4.31RR50 pKa = 11.84LANSEE55 pKa = 4.64DD56 pKa = 2.95IANFVFCRR64 pKa = 11.84LFYY67 pKa = 9.95QGRR70 pKa = 11.84WACCIRR76 pKa = 11.84QGRR79 pKa = 11.84VLRR82 pKa = 11.84RR83 pKa = 11.84DD84 pKa = 2.94KK85 pKa = 10.81RR86 pKa = 11.84RR87 pKa = 11.84QVEE90 pKa = 4.17DD91 pKa = 2.98RR92 pKa = 11.84CTRR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 10.21RR98 pKa = 11.84IFQTSHH104 pKa = 7.0DD105 pKa = 4.13SSFSRR110 pKa = 4.49

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9237

0

9237

4072588

9

6727

440.9

49.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.117 ± 0.024

1.795 ± 0.011

5.468 ± 0.015

6.282 ± 0.022

3.971 ± 0.016

5.472 ± 0.025

2.579 ± 0.012

4.889 ± 0.017

5.538 ± 0.027

9.886 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.009

4.001 ± 0.013

4.325 ± 0.02

4.232 ± 0.018

6.008 ± 0.02

8.317 ± 0.026

5.78 ± 0.022

6.908 ± 0.017

1.149 ± 0.007

2.699 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski