Streptococcus phage Javan242

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZH6|A0A4D6AZH6_9CAUD Major tail protein OS=Streptococcus phage Javan242 OX=2548066 GN=Javan242_0016 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 4.56KK3 pKa = 11.2VKK5 pKa = 10.77LFQNEE10 pKa = 4.09VLGSGFDD17 pKa = 3.23IDD19 pKa = 6.24GYY21 pKa = 10.9FGWQCWDD28 pKa = 3.58GYY30 pKa = 11.61AKK32 pKa = 10.42YY33 pKa = 10.01CLWLGVPFANCMVSGYY49 pKa = 10.94VKK51 pKa = 10.45DD52 pKa = 3.59LWEE55 pKa = 3.61QRR57 pKa = 11.84YY58 pKa = 10.2NNGILDD64 pKa = 3.88YY65 pKa = 10.65FDD67 pKa = 4.26EE68 pKa = 4.56VEE70 pKa = 4.04NLEE73 pKa = 4.22GGEE76 pKa = 3.96VVIFTEE82 pKa = 4.55NEE84 pKa = 3.8WTPVSHH90 pKa = 6.3VAVFVADD97 pKa = 3.41IDD99 pKa = 4.22GTQGWFLGQNQGGEE113 pKa = 4.09AGPNGGGAFNLVAFPYY129 pKa = 9.01STLYY133 pKa = 8.37PTAFRR138 pKa = 11.84PKK140 pKa = 10.53GEE142 pKa = 4.23SLPKK146 pKa = 10.07QEE148 pKa = 4.58LKK150 pKa = 10.87EE151 pKa = 4.5AITEE155 pKa = 3.95VMEE158 pKa = 3.89NHH160 pKa = 6.55EE161 pKa = 4.39APFYY165 pKa = 10.86PEE167 pKa = 4.96DD168 pKa = 3.44ATFTVGDD175 pKa = 3.59SPINVRR181 pKa = 11.84RR182 pKa = 11.84EE183 pKa = 3.64PSLTGEE189 pKa = 4.08IVAVYY194 pKa = 9.88QPGEE198 pKa = 4.07KK199 pKa = 9.62VHH201 pKa = 6.35YY202 pKa = 9.59DD203 pKa = 3.34SKK205 pKa = 11.38GSNDD209 pKa = 3.13GYY211 pKa = 11.06RR212 pKa = 11.84WISYY216 pKa = 9.87VGEE219 pKa = 4.28SGNRR223 pKa = 11.84NYY225 pKa = 10.82LAIGQTDD232 pKa = 3.5EE233 pKa = 4.12AGNRR237 pKa = 11.84IDD239 pKa = 4.83LWGEE243 pKa = 3.77LSS245 pKa = 3.33

Molecular weight: 27.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AW92|A0A4D6AW92_9CAUD Site-specific recombinase OS=Streptococcus phage Javan242 OX=2548066 GN=Javan242_0028 PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 10.11SGPMQKK8 pKa = 9.81VLSQYY13 pKa = 11.31ASDD16 pKa = 3.31IQARR20 pKa = 11.84CGDD23 pKa = 4.08GYY25 pKa = 11.6VKK27 pKa = 10.45DD28 pKa = 3.58VHH30 pKa = 7.32VGKK33 pKa = 10.15NRR35 pKa = 11.84ANAMVRR41 pKa = 11.84AKK43 pKa = 9.17TRR45 pKa = 11.84KK46 pKa = 9.31AKK48 pKa = 10.23KK49 pKa = 10.29DD50 pKa = 3.33NLKK53 pKa = 11.11NNTLLKK59 pKa = 10.42AVRR62 pKa = 3.79

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9788

43

1292

191.9

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.835 ± 0.535

0.715 ± 0.104

5.966 ± 0.217

8.143 ± 0.501

4.219 ± 0.253

6.937 ± 0.495

1.328 ± 0.148

7.274 ± 0.286

8.715 ± 0.436

8.112 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.205

5.67 ± 0.201

2.483 ± 0.222

3.893 ± 0.225

4.332 ± 0.336

6.161 ± 0.265

5.609 ± 0.317

6.079 ± 0.229

1.461 ± 0.22

3.79 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski