Rhizobium sp. ADMK78

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Peteryoungia

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R9KNV6|A0A5R9KNV6_9RHIZ Uncharacterized protein OS=Rhizobium sp. ADMK78 OX=1813451 GN=FE840_13375 PE=4 SV=1
MM1 pKa = 7.95AITGDD6 pKa = 3.24IKK8 pKa = 10.99FDD10 pKa = 3.49DD11 pKa = 4.09FEE13 pKa = 4.89IVFEE17 pKa = 4.5NGEE20 pKa = 4.15KK21 pKa = 10.55LAFDD25 pKa = 4.25ALVADD30 pKa = 4.94RR31 pKa = 11.84FIADD35 pKa = 3.95GQSVPASVYY44 pKa = 10.68SVAPSAAPEE53 pKa = 4.07LLNGNRR59 pKa = 11.84LCGNEE64 pKa = 4.01PVTFLASWIDD74 pKa = 3.52GDD76 pKa = 3.89VTAIAVFDD84 pKa = 3.93TPEE87 pKa = 3.99QPEE90 pKa = 4.25SDD92 pKa = 3.21ATMCALYY99 pKa = 9.72TYY101 pKa = 9.71VYY103 pKa = 10.06PP104 pKa = 5.13

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R9LBK6|A0A5R9LBK6_9RHIZ Helix-turn-helix transcriptional regulator OS=Rhizobium sp. ADMK78 OX=1813451 GN=FE840_01275 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.22GGRR28 pKa = 11.84KK29 pKa = 9.06VLSARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84SRR39 pKa = 11.84KK40 pKa = 9.43RR41 pKa = 11.84LSAA44 pKa = 3.85

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4000

0

4000

1255675

32

3370

313.9

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.501 ± 0.044

0.826 ± 0.013

5.746 ± 0.035

6.054 ± 0.036

3.917 ± 0.025

8.22 ± 0.037

2.081 ± 0.021

5.76 ± 0.026

3.644 ± 0.034

10.076 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.019

2.842 ± 0.026

4.809 ± 0.029

3.24 ± 0.02

6.698 ± 0.041

5.814 ± 0.031

5.289 ± 0.026

7.288 ± 0.034

1.239 ± 0.017

2.254 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski