Dictyobacter sp. Uno17
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5628 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5A5TKA2|A0A5A5TKA2_9CHLR Uncharacterized protein OS=Dictyobacter sp. Uno17 OX=2014874 GN=yisX PE=4 SV=1
MM1 pKa = 7.64 QEE3 pKa = 3.82 ALKK6 pKa = 10.81 ANPYY10 pKa = 10.17 VYY12 pKa = 11.04 ADD14 pKa = 3.63 NNPVNEE20 pKa = 4.05 VDD22 pKa = 3.6 PNGAFSLTDD31 pKa = 3.05 ACLVDD36 pKa = 4.74 LVGLGGITSAVTGLFSFLGSSGAGGAVGIPLAAATGPGDD75 pKa = 3.52 VAIAGLAASLFIGLAVTSSIIGIIGVIEE103 pKa = 4.07 GVPTAKK109 pKa = 10.04 KK110 pKa = 10.62 DD111 pKa = 3.46 CGLL114 pKa = 3.8
Molecular weight: 11.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.884
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 1.901
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A5A5T5V4|A0A5A5T5V4_9CHLR NADH-quinone oxidoreductase subunit K OS=Dictyobacter sp. Uno17 OX=2014874 GN=nuoK1 PE=3 SV=1
MM1 pKa = 7.34 SVALSPTFQVATIKK15 pKa = 10.32 MLTIPTTIRR24 pKa = 11.84 PSRR27 pKa = 11.84 KK28 pKa = 8.35 RR29 pKa = 11.84 LRR31 pKa = 11.84 ALRR34 pKa = 11.84 VLSQRR39 pKa = 11.84 LSIGLTPHH47 pKa = 7.02
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5628
0
5628
1921236
40
7655
341.4
37.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.274 ± 0.034
0.964 ± 0.01
4.83 ± 0.023
5.752 ± 0.037
3.595 ± 0.02
6.837 ± 0.032
2.713 ± 0.02
6.15 ± 0.026
3.383 ± 0.026
10.99 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.013
3.497 ± 0.026
5.028 ± 0.027
5.412 ± 0.035
5.76 ± 0.033
6.238 ± 0.027
6.211 ± 0.029
6.593 ± 0.03
1.4 ± 0.013
3.141 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here