Cyanophage NATL1A-7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Cheungvirus; Prochlorococcus virus NATL1A7

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SNA1|E3SNA1_9CAUD Predicted protein OS=Cyanophage NATL1A-7 OX=445693 GN=CYIG_00032 PE=4 SV=1
MM1 pKa = 7.17TKK3 pKa = 10.41YY4 pKa = 9.52MCFLEE9 pKa = 4.19SGRR12 pKa = 11.84DD13 pKa = 3.72FVITADD19 pKa = 4.15DD20 pKa = 4.75DD21 pKa = 3.42MDD23 pKa = 4.13AAYY26 pKa = 7.76TAQAYY31 pKa = 7.5AAWMYY36 pKa = 11.51DD37 pKa = 3.65DD38 pKa = 5.33YY39 pKa = 11.48LTDD42 pKa = 4.74LEE44 pKa = 5.36PIHH47 pKa = 7.19DD48 pKa = 3.93VV49 pKa = 3.35

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SNC2|E3SNC2_9CAUD Predicted protein OS=Cyanophage NATL1A-7 OX=445693 GN=CYIG_00053 PE=4 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84SITRR7 pKa = 11.84KK8 pKa = 7.11QQEE11 pKa = 4.24RR12 pKa = 11.84NKK14 pKa = 9.14KK15 pKa = 6.73TKK17 pKa = 9.9KK18 pKa = 9.87KK19 pKa = 10.13KK20 pKa = 7.03QTNRR24 pKa = 11.84EE25 pKa = 3.94KK26 pKa = 10.88LQAAIPGVMYY36 pKa = 10.37KK37 pKa = 10.87GSGKK41 pKa = 10.33DD42 pKa = 3.12PAVRR46 pKa = 11.84EE47 pKa = 4.03LLKK50 pKa = 10.71KK51 pKa = 10.4LKK53 pKa = 10.25KK54 pKa = 10.29DD55 pKa = 3.62GPGSLDD61 pKa = 3.22SSGMGTRR68 pKa = 11.84WKK70 pKa = 10.71VV71 pKa = 2.86

Molecular weight: 8.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13310

39

1525

204.8

22.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.663 ± 0.364

0.781 ± 0.137

6.822 ± 0.308

6.386 ± 0.364

3.388 ± 0.229

7.002 ± 0.282

1.796 ± 0.123

5.605 ± 0.311

6.844 ± 0.51

7.603 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.235

5.597 ± 0.267

3.952 ± 0.299

4.035 ± 0.259

4.162 ± 0.346

7.025 ± 0.513

7.19 ± 0.548

5.612 ± 0.265

1.307 ± 0.148

3.862 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski