Amino acid dipepetide frequency for Pseudomonas sp. FeS53a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.808AlaAla: 13.808 ± 0.119	1.434AlaCys: 1.434 ± 0.033	6.309AlaAsp: 6.309 ± 0.066	7.846AlaGlu: 7.846 ± 0.091	4.148AlaPhe: 4.148 ± 0.052	9.913AlaGly: 9.913 ± 0.083	2.437AlaHis: 2.437 ± 0.04	4.814AlaIle: 4.814 ± 0.06	3.095AlaLys: 3.095 ± 0.058	15.683AlaLeu: 15.683 ± 0.141	2.846AlaMet: 2.846 ± 0.047	2.762AlaAsn: 2.762 ± 0.044	5.596AlaPro: 5.596 ± 0.069	5.067AlaGln: 5.067 ± 0.059	9.181AlaArg: 9.181 ± 0.097	6.255AlaSer: 6.255 ± 0.065	5.004AlaThr: 5.004 ± 0.074	7.652AlaVal: 7.652 ± 0.074	1.929AlaTrp: 1.929 ± 0.039	2.622AlaTyr: 2.622 ± 0.041	0.007AlaXaa: 0.007 ± 0.002
Cys	1.1CysAla: 1.1 ± 0.03	0.13CysCys: 0.13 ± 0.009	0.523CysAsp: 0.523 ± 0.018	0.56CysGlu: 0.56 ± 0.019	0.331CysPhe: 0.331 ± 0.013	0.931CysGly: 0.931 ± 0.022	0.271CysHis: 0.271 ± 0.012	0.401CysIle: 0.401 ± 0.017	0.235CysLys: 0.235 ± 0.012	1.164CysLeu: 1.164 ± 0.023	0.184CysMet: 0.184 ± 0.01	0.221CysAsn: 0.221 ± 0.011	0.492CysPro: 0.492 ± 0.019	0.415CysGln: 0.415 ± 0.014	0.708CysArg: 0.708 ± 0.022	0.567CysSer: 0.567 ± 0.017	0.463CysThr: 0.463 ± 0.018	0.648CysVal: 0.648 ± 0.023	0.149CysTrp: 0.149 ± 0.009	0.24CysTyr: 0.24 ± 0.012	0.001CysXaa: 0.001 ± 0.001
Asp	6.674AspAla: 6.674 ± 0.07	0.521AspCys: 0.521 ± 0.017	3.048AspAsp: 3.048 ± 0.046	3.577AspGlu: 3.577 ± 0.055	2.057AspPhe: 2.057 ± 0.033	4.999AspGly: 4.999 ± 0.084	1.21AspHis: 1.21 ± 0.028	2.384AspIle: 2.384 ± 0.04	1.558AspLys: 1.558 ± 0.038	6.396AspLeu: 6.396 ± 0.063	1.077AspMet: 1.077 ± 0.021	1.401AspAsn: 1.401 ± 0.037	3.104AspPro: 3.104 ± 0.037	2.048AspGln: 2.048 ± 0.033	3.367AspArg: 3.367 ± 0.047	2.703AspSer: 2.703 ± 0.05	2.384AspThr: 2.384 ± 0.047	3.437AspVal: 3.437 ± 0.052	1.018AspTrp: 1.018 ± 0.021	1.738AspTyr: 1.738 ± 0.04	0.001AspXaa: 0.001 ± 0.001
Glu	7.896GluAla: 7.896 ± 0.1	0.41GluCys: 0.41 ± 0.017	2.675GluAsp: 2.675 ± 0.044	3.302GluGlu: 3.302 ± 0.047	1.628GluPhe: 1.628 ± 0.031	4.63GluGly: 4.63 ± 0.05	1.602GluHis: 1.602 ± 0.033	2.437GluIle: 2.437 ± 0.044	1.794GluLys: 1.794 ± 0.042	7.166GluLeu: 7.166 ± 0.074	1.248GluMet: 1.248 ± 0.03	1.376GluAsn: 1.376 ± 0.029	2.872GluPro: 2.872 ± 0.053	3.574GluGln: 3.574 ± 0.055	5.711GluArg: 5.711 ± 0.079	2.56GluSer: 2.56 ± 0.037	2.45GluThr: 2.45 ± 0.036	4.412GluVal: 4.412 ± 0.043	0.702GluTrp: 0.702 ± 0.018	1.136GluTyr: 1.136 ± 0.026	0.003GluXaa: 0.003 ± 0.001
Phe	4.076PheAla: 4.076 ± 0.05	0.443PheCys: 0.443 ± 0.017	2.399PheAsp: 2.399 ± 0.036	2.048PheGlu: 2.048 ± 0.036	1.375PhePhe: 1.375 ± 0.036	3.174PheGly: 3.174 ± 0.051	0.782PheHis: 0.782 ± 0.02	1.677PheIle: 1.677 ± 0.032	1.021PheLys: 1.021 ± 0.024	3.274PheLeu: 3.274 ± 0.047	0.739PheMet: 0.739 ± 0.023	1.156PheAsn: 1.156 ± 0.025	1.49PhePro: 1.49 ± 0.03	1.241PheGln: 1.241 ± 0.027	1.969PheArg: 1.969 ± 0.036	2.259PheSer: 2.259 ± 0.045	1.915PheThr: 1.915 ± 0.036	2.401PheVal: 2.401 ± 0.039	0.567PheTrp: 0.567 ± 0.022	0.972PheTyr: 0.972 ± 0.026	0.001PheXaa: 0.001 ± 0.001
Gly	8.083GlyAla: 8.083 ± 0.086	0.977GlyCys: 0.977 ± 0.026	4.188GlyAsp: 4.188 ± 0.054	5.333GlyGlu: 5.333 ± 0.062	3.346GlyPhe: 3.346 ± 0.045	6.568GlyGly: 6.568 ± 0.093	2.041GlyHis: 2.041 ± 0.035	3.891GlyIle: 3.891 ± 0.047	2.892GlyLys: 2.892 ± 0.052	10.291GlyLeu: 10.291 ± 0.091	2.108GlyMet: 2.108 ± 0.04	2.319GlyAsn: 2.319 ± 0.053	2.933GlyPro: 2.933 ± 0.049	3.957GlyGln: 3.957 ± 0.051	5.814GlyArg: 5.814 ± 0.059	4.629GlySer: 4.629 ± 0.088	3.999GlyThr: 3.999 ± 0.079	6.054GlyVal: 6.054 ± 0.069	1.411GlyTrp: 1.411 ± 0.03	2.529GlyTyr: 2.529 ± 0.041	0.006GlyXaa: 0.006 ± 0.002
His	2.449HisAla: 2.449 ± 0.046	0.317HisCys: 0.317 ± 0.014	1.243HisAsp: 1.243 ± 0.028	1.203HisGlu: 1.203 ± 0.031	0.958HisPhe: 0.958 ± 0.023	2.063HisGly: 2.063 ± 0.038	0.674HisHis: 0.674 ± 0.018	0.898HisIle: 0.898 ± 0.022	0.532HisLys: 0.532 ± 0.017	2.953HisLeu: 2.953 ± 0.047	0.477HisMet: 0.477 ± 0.018	0.552HisAsn: 0.552 ± 0.02	1.598HisPro: 1.598 ± 0.035	0.936HisGln: 0.936 ± 0.025	1.522HisArg: 1.522 ± 0.027	1.126HisSer: 1.126 ± 0.023	0.918HisThr: 0.918 ± 0.024	1.265HisVal: 1.265 ± 0.026	0.494HisTrp: 0.494 ± 0.017	0.787HisTyr: 0.787 ± 0.023	0.001HisXaa: 0.001 ± 0.001
Ile	5.271IleAla: 5.271 ± 0.059	0.371IleCys: 0.371 ± 0.013	2.848IleAsp: 2.848 ± 0.04	2.901IleGlu: 2.901 ± 0.049	1.108IlePhe: 1.108 ± 0.032	4.122IleGly: 4.122 ± 0.052	0.908IleHis: 0.908 ± 0.024	1.499IleIle: 1.499 ± 0.033	1.323IleLys: 1.323 ± 0.031	3.634IleLeu: 3.634 ± 0.051	0.603IleMet: 0.603 ± 0.02	1.371IleAsn: 1.371 ± 0.032	2.07IlePro: 2.07 ± 0.033	1.451IleGln: 1.451 ± 0.033	2.947IleArg: 2.947 ± 0.041	2.347IleSer: 2.347 ± 0.04	2.273IleThr: 2.273 ± 0.042	2.562IleVal: 2.562 ± 0.041	0.436IleTrp: 0.436 ± 0.016	0.938IleTyr: 0.938 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.817LysAla: 3.817 ± 0.052	0.137LysCys: 0.137 ± 0.009	1.539LysAsp: 1.539 ± 0.029	1.398LysGlu: 1.398 ± 0.034	0.663LysPhe: 0.663 ± 0.023	2.418LysGly: 2.418 ± 0.044	0.564LysHis: 0.564 ± 0.019	1.107LysIle: 1.107 ± 0.03	1.016LysLys: 1.016 ± 0.037	3.067LysLeu: 3.067 ± 0.05	0.531LysMet: 0.531 ± 0.021	0.736LysAsn: 0.736 ± 0.025	1.828LysPro: 1.828 ± 0.038	1.233LysGln: 1.233 ± 0.028	2.13LysArg: 2.13 ± 0.037	1.307LysSer: 1.307 ± 0.032	1.411LysThr: 1.411 ± 0.031	2.437LysVal: 2.437 ± 0.046	0.281LysTrp: 0.281 ± 0.012	0.556LysTyr: 0.556 ± 0.018	0.001LysXaa: 0.001 ± 0.001
Leu	16.706LeuAla: 16.706 ± 0.134	1.236LeuCys: 1.236 ± 0.028	7.592LeuAsp: 7.592 ± 0.074	7.23LeuGlu: 7.23 ± 0.076	4.206LeuPhe: 4.206 ± 0.052	10.165LeuGly: 10.165 ± 0.099	2.594LeuHis: 2.594 ± 0.041	4.542LeuIle: 4.542 ± 0.058	3.828LeuLys: 3.828 ± 0.053	14.4LeuLeu: 14.4 ± 0.152	2.508LeuMet: 2.508 ± 0.043	3.209LeuAsn: 3.209 ± 0.045	6.944LeuPro: 6.944 ± 0.075	4.616LeuGln: 4.616 ± 0.056	8.485LeuArg: 8.485 ± 0.09	6.543LeuSer: 6.543 ± 0.063	5.364LeuThr: 5.364 ± 0.068	8.848LeuVal: 8.848 ± 0.084	1.42LeuTrp: 1.42 ± 0.031	2.677LeuTyr: 2.677 ± 0.039	0.003LeuXaa: 0.003 ± 0.001
Met	2.583MetAla: 2.583 ± 0.045	0.14MetCys: 0.14 ± 0.009	1.002MetAsp: 1.002 ± 0.024	0.976MetGlu: 0.976 ± 0.025	0.578MetPhe: 0.578 ± 0.02	1.587MetGly: 1.587 ± 0.036	0.453MetHis: 0.453 ± 0.017	0.881MetIle: 0.881 ± 0.028	0.783MetLys: 0.783 ± 0.022	2.434MetLeu: 2.434 ± 0.04	0.402MetMet: 0.402 ± 0.017	0.743MetAsn: 0.743 ± 0.021	1.309MetPro: 1.309 ± 0.026	0.933MetGln: 0.933 ± 0.024	1.498MetArg: 1.498 ± 0.025	1.507MetSer: 1.507 ± 0.027	1.286MetThr: 1.286 ± 0.03	1.405MetVal: 1.405 ± 0.032	0.164MetTrp: 0.164 ± 0.01	0.306MetTyr: 0.306 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.995AsnAla: 2.995 ± 0.051	0.245AsnCys: 0.245 ± 0.012	1.397AsnAsp: 1.397 ± 0.041	1.298AsnGlu: 1.298 ± 0.033	0.895AsnPhe: 0.895 ± 0.025	2.468AsnGly: 2.468 ± 0.064	0.55AsnHis: 0.55 ± 0.018	1.089AsnIle: 1.089 ± 0.026	0.695AsnLys: 0.695 ± 0.019	3.324AsnLeu: 3.324 ± 0.046	0.445AsnMet: 0.445 ± 0.017	0.719AsnAsn: 0.719 ± 0.026	1.84AsnPro: 1.84 ± 0.035	1.133AsnGln: 1.133 ± 0.027	1.894AsnArg: 1.894 ± 0.035	1.239AsnSer: 1.239 ± 0.03	1.263AsnThr: 1.263 ± 0.028	1.676AsnVal: 1.676 ± 0.04	0.408AsnTrp: 0.408 ± 0.016	0.697AsnTyr: 0.697 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	6.553ProAla: 6.553 ± 0.073	0.382ProCys: 0.382 ± 0.017	3.058ProAsp: 3.058 ± 0.051	3.773ProGlu: 3.773 ± 0.053	1.878ProPhe: 1.878 ± 0.032	4.544ProGly: 4.544 ± 0.057	1.095ProHis: 1.095 ± 0.024	1.781ProIle: 1.781 ± 0.032	1.324ProLys: 1.324 ± 0.029	6.281ProLeu: 6.281 ± 0.076	1.145ProMet: 1.145 ± 0.026	1.257ProAsn: 1.257 ± 0.032	2.294ProPro: 2.294 ± 0.043	2.189ProGln: 2.189 ± 0.035	3.323ProArg: 3.323 ± 0.052	2.687ProSer: 2.687 ± 0.039	2.235ProThr: 2.235 ± 0.041	3.977ProVal: 3.977 ± 0.055	0.838ProTrp: 0.838 ± 0.024	1.168ProTyr: 1.168 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	6.429GlnAla: 6.429 ± 0.08	0.304GlnCys: 0.304 ± 0.014	1.925GlnAsp: 1.925 ± 0.037	1.867GlnGlu: 1.867 ± 0.035	1.225GlnPhe: 1.225 ± 0.026	3.644GlnGly: 3.644 ± 0.057	1.068GlnHis: 1.068 ± 0.023	1.524GlnIle: 1.524 ± 0.03	1.024GlnLys: 1.024 ± 0.025	5.239GlnLeu: 5.239 ± 0.056	0.915GlnMet: 0.915 ± 0.026	0.919GlnAsn: 0.919 ± 0.024	2.599GlnPro: 2.599 ± 0.043	2.418GlnGln: 2.418 ± 0.049	4.105GlnArg: 4.105 ± 0.062	1.884GlnSer: 1.884 ± 0.038	1.554GlnThr: 1.554 ± 0.033	3.851GlnVal: 3.851 ± 0.056	0.611GlnTrp: 0.611 ± 0.019	0.894GlnTyr: 0.894 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	7.27ArgAla: 7.27 ± 0.083	0.654ArgCys: 0.654 ± 0.022	4.029ArgAsp: 4.029 ± 0.054	4.947ArgGlu: 4.947 ± 0.072	3.067ArgPhe: 3.067 ± 0.043	4.535ArgGly: 4.535 ± 0.05	2.098ArgHis: 2.098 ± 0.04	3.405ArgIle: 3.405 ± 0.051	1.836ArgLys: 1.836 ± 0.034	10.13ArgLeu: 10.13 ± 0.108	1.629ArgMet: 1.629 ± 0.03	1.924ArgAsn: 1.924 ± 0.037	3.442ArgPro: 3.442 ± 0.05	4.0ArgGln: 4.0 ± 0.05	5.348ArgArg: 5.348 ± 0.069	3.472ArgSer: 3.472 ± 0.048	3.103ArgThr: 3.103 ± 0.047	5.04ArgVal: 5.04 ± 0.058	1.209ArgTrp: 1.209 ± 0.028	2.253ArgTyr: 2.253 ± 0.035	0.004ArgXaa: 0.004 ± 0.001
Ser	5.834SerAla: 5.834 ± 0.067	0.451SerCys: 0.451 ± 0.018	2.594SerAsp: 2.594 ± 0.056	2.773SerGlu: 2.773 ± 0.045	1.902SerPhe: 1.902 ± 0.039	5.149SerGly: 5.149 ± 0.084	1.196SerHis: 1.196 ± 0.028	2.223SerIle: 2.223 ± 0.042	1.235SerLys: 1.235 ± 0.03	6.934SerLeu: 6.934 ± 0.076	1.094SerMet: 1.094 ± 0.024	1.426SerAsn: 1.426 ± 0.038	2.727SerPro: 2.727 ± 0.044	2.148SerGln: 2.148 ± 0.038	3.718SerArg: 3.718 ± 0.046	2.901SerSer: 2.901 ± 0.062	2.592SerThr: 2.592 ± 0.05	3.535SerVal: 3.535 ± 0.058	0.719SerTrp: 0.719 ± 0.021	1.272SerTyr: 1.272 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	4.947ThrAla: 4.947 ± 0.068	0.465ThrCys: 0.465 ± 0.017	2.247ThrAsp: 2.247 ± 0.048	2.28ThrGlu: 2.28 ± 0.037	1.672ThrPhe: 1.672 ± 0.032	4.163ThrGly: 4.163 ± 0.068	1.019ThrHis: 1.019 ± 0.024	1.559ThrIle: 1.559 ± 0.048	0.831ThrLys: 0.831 ± 0.026	6.832ThrLeu: 6.832 ± 0.09	0.634ThrMet: 0.634 ± 0.02	1.013ThrAsn: 1.013 ± 0.027	3.143ThrPro: 3.143 ± 0.048	1.805ThrGln: 1.805 ± 0.035	3.397ThrArg: 3.397 ± 0.045	2.366ThrSer: 2.366 ± 0.054	2.253ThrThr: 2.253 ± 0.053	3.209ThrVal: 3.209 ± 0.065	0.75ThrTrp: 0.75 ± 0.022	1.183ThrTyr: 1.183 ± 0.031	0.002ThrXaa: 0.002 ± 0.001
Val	8.132ValAla: 8.132 ± 0.071	0.685ValCys: 0.685 ± 0.022	4.074ValAsp: 4.074 ± 0.059	4.536ValGlu: 4.536 ± 0.056	2.473ValPhe: 2.473 ± 0.039	5.269ValGly: 5.269 ± 0.066	1.444ValHis: 1.444 ± 0.032	3.425ValIle: 3.425 ± 0.044	2.077ValLys: 2.077 ± 0.041	8.516ValLeu: 8.516 ± 0.082	1.61ValMet: 1.61 ± 0.034	2.023ValAsn: 2.023 ± 0.044	3.463ValPro: 3.463 ± 0.049	2.692ValGln: 2.692 ± 0.037	4.808ValArg: 4.808 ± 0.048	3.876ValSer: 3.876 ± 0.051	3.536ValThr: 3.536 ± 0.07	5.407ValVal: 5.407 ± 0.073	0.814ValTrp: 0.814 ± 0.023	1.474ValTyr: 1.474 ± 0.03	0.002ValXaa: 0.002 ± 0.001
Trp	1.277TrpAla: 1.277 ± 0.027	0.159TrpCys: 0.159 ± 0.009	0.655TrpAsp: 0.655 ± 0.021	0.584TrpGlu: 0.584 ± 0.02	0.535TrpPhe: 0.535 ± 0.019	0.95TrpGly: 0.95 ± 0.024	0.413TrpHis: 0.413 ± 0.015	0.59TrpIle: 0.59 ± 0.018	0.401TrpLys: 0.401 ± 0.015	2.304TrpLeu: 2.304 ± 0.039	0.354TrpMet: 0.354 ± 0.013	0.46TrpAsn: 0.46 ± 0.018	0.709TrpPro: 0.709 ± 0.021	0.92TrpGln: 0.92 ± 0.026	1.256TrpArg: 1.256 ± 0.026	0.78TrpSer: 0.78 ± 0.023	0.624TrpThr: 0.624 ± 0.02	0.986TrpVal: 0.986 ± 0.026	0.231TrpTrp: 0.231 ± 0.011	0.345TrpTyr: 0.345 ± 0.015	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.504TyrAla: 2.504 ± 0.039	0.274TyrCys: 0.274 ± 0.014	1.356TyrAsp: 1.356 ± 0.033	1.15TyrGlu: 1.15 ± 0.031	0.936TyrPhe: 0.936 ± 0.025	2.075TyrGly: 2.075 ± 0.039	0.547TyrHis: 0.547 ± 0.019	0.854TyrIle: 0.854 ± 0.023	0.675TyrLys: 0.675 ± 0.023	3.085TyrLeu: 3.085 ± 0.037	0.42TyrMet: 0.42 ± 0.017	0.686TyrAsn: 0.686 ± 0.023	1.322TyrPro: 1.322 ± 0.027	1.223TyrGln: 1.223 ± 0.029	2.212TyrArg: 2.212 ± 0.037	1.412TyrSer: 1.412 ± 0.032	1.141TyrThr: 1.141 ± 0.028	1.532TyrVal: 1.532 ± 0.029	0.383TyrTrp: 0.383 ± 0.016	0.681TyrTyr: 0.681 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.006XaaLeu: 0.006 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.005XaaPro: 0.005 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.004XaaSer: 0.004 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 5389 proteins (1739345 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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