Amino acid dipepetide frequency for Thiomicrospira sp. WB1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.193AlaAla: 8.193 ± 0.158
0.882AlaCys: 0.882 ± 0.042
5.464AlaAsp: 5.464 ± 0.103
6.058AlaGlu: 6.058 ± 0.114
3.929AlaPhe: 3.929 ± 0.081
6.48AlaGly: 6.48 ± 0.115
2.359AlaHis: 2.359 ± 0.06
5.176AlaIle: 5.176 ± 0.089
4.358AlaLys: 4.358 ± 0.09
11.906AlaLeu: 11.906 ± 0.18
3.146AlaMet: 3.146 ± 0.071
2.959AlaAsn: 2.959 ± 0.086
3.646AlaPro: 3.646 ± 0.085
5.804AlaGln: 5.804 ± 0.108
5.069AlaArg: 5.069 ± 0.092
5.543AlaSer: 5.543 ± 0.105
4.492AlaThr: 4.492 ± 0.088
6.218AlaVal: 6.218 ± 0.117
1.662AlaTrp: 1.662 ± 0.058
2.687AlaTyr: 2.687 ± 0.063
0.0AlaXaa: 0.0 ± 0.0
Cys
0.667CysAla: 0.667 ± 0.035
0.064CysCys: 0.064 ± 0.011
0.584CysAsp: 0.584 ± 0.03
0.561CysGlu: 0.561 ± 0.029
0.311CysPhe: 0.311 ± 0.019
0.695CysGly: 0.695 ± 0.033
0.321CysHis: 0.321 ± 0.025
0.339CysIle: 0.339 ± 0.024
0.228CysLys: 0.228 ± 0.019
0.921CysLeu: 0.921 ± 0.041
0.161CysMet: 0.161 ± 0.016
0.183CysAsn: 0.183 ± 0.017
0.469CysPro: 0.469 ± 0.032
0.526CysGln: 0.526 ± 0.03
0.477CysArg: 0.477 ± 0.027
0.362CysSer: 0.362 ± 0.024
0.32CysThr: 0.32 ± 0.021
0.525CysVal: 0.525 ± 0.03
0.067CysTrp: 0.067 ± 0.009
0.186CysTyr: 0.186 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
5.734AspAla: 5.734 ± 0.095
0.477AspCys: 0.477 ± 0.027
3.366AspAsp: 3.366 ± 0.118
4.076AspGlu: 4.076 ± 0.089
2.55AspPhe: 2.55 ± 0.059
3.775AspGly: 3.775 ± 0.099
1.353AspHis: 1.353 ± 0.047
2.997AspIle: 2.997 ± 0.071
2.382AspLys: 2.382 ± 0.063
6.031AspLeu: 6.031 ± 0.095
1.51AspMet: 1.51 ± 0.051
1.814AspAsn: 1.814 ± 0.053
2.831AspPro: 2.831 ± 0.062
3.655AspGln: 3.655 ± 0.075
3.229AspArg: 3.229 ± 0.076
2.771AspSer: 2.771 ± 0.073
3.007AspThr: 3.007 ± 0.081
3.992AspVal: 3.992 ± 0.075
1.286AspTrp: 1.286 ± 0.047
2.005AspTyr: 2.005 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
7.172GluAla: 7.172 ± 0.119
0.398GluCys: 0.398 ± 0.025
2.983GluAsp: 2.983 ± 0.081
3.528GluGlu: 3.528 ± 0.102
1.911GluPhe: 1.911 ± 0.056
3.922GluGly: 3.922 ± 0.077
1.539GluHis: 1.539 ± 0.047
3.344GluIle: 3.344 ± 0.073
3.556GluLys: 3.556 ± 0.082
5.81GluLeu: 5.81 ± 0.104
1.748GluMet: 1.748 ± 0.051
2.261GluAsn: 2.261 ± 0.065
2.563GluPro: 2.563 ± 0.081
3.919GluGln: 3.919 ± 0.086
3.996GluArg: 3.996 ± 0.081
3.441GluSer: 3.441 ± 0.079
4.213GluThr: 4.213 ± 0.071
4.408GluVal: 4.408 ± 0.093
0.709GluTrp: 0.709 ± 0.034
1.444GluTyr: 1.444 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.379PheAla: 3.379 ± 0.069
0.378PheCys: 0.378 ± 0.026
2.969PheAsp: 2.969 ± 0.073
3.082PheGlu: 3.082 ± 0.066
1.67PhePhe: 1.67 ± 0.056
2.86PheGly: 2.86 ± 0.083
0.87PheHis: 0.87 ± 0.036
1.867PheIle: 1.867 ± 0.059
1.636PheLys: 1.636 ± 0.047
3.594PheLeu: 3.594 ± 0.077
1.0PheMet: 1.0 ± 0.036
1.402PheAsn: 1.402 ± 0.048
1.321PhePro: 1.321 ± 0.042
1.652PheGln: 1.652 ± 0.052
1.603PheArg: 1.603 ± 0.049
2.46PheSer: 2.46 ± 0.058
1.782PheThr: 1.782 ± 0.053
2.63PheVal: 2.63 ± 0.058
0.663PheTrp: 0.663 ± 0.033
1.414PheTyr: 1.414 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.628GlyAla: 5.628 ± 0.106
0.735GlyCys: 0.735 ± 0.029
3.726GlyAsp: 3.726 ± 0.082
4.448GlyGlu: 4.448 ± 0.086
3.149GlyPhe: 3.149 ± 0.075
4.665GlyGly: 4.665 ± 0.121
1.93GlyHis: 1.93 ± 0.06
3.8GlyIle: 3.8 ± 0.077
3.085GlyLys: 3.085 ± 0.068
8.126GlyLeu: 8.126 ± 0.127
2.146GlyMet: 2.146 ± 0.072
1.93GlyAsn: 1.93 ± 0.068
2.104GlyPro: 2.104 ± 0.065
3.906GlyGln: 3.906 ± 0.077
3.81GlyArg: 3.81 ± 0.074
3.293GlySer: 3.293 ± 0.081
3.414GlyThr: 3.414 ± 0.082
5.262GlyVal: 5.262 ± 0.1
1.19GlyTrp: 1.19 ± 0.05
2.348GlyTyr: 2.348 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
2.206HisAla: 2.206 ± 0.057
0.311HisCys: 0.311 ± 0.023
1.261HisAsp: 1.261 ± 0.044
1.266HisGlu: 1.266 ± 0.044
1.196HisPhe: 1.196 ± 0.041
1.726HisGly: 1.726 ± 0.055
0.888HisHis: 0.888 ± 0.033
1.177HisIle: 1.177 ± 0.042
0.952HisLys: 0.952 ± 0.042
2.863HisLeu: 2.863 ± 0.066
0.484HisMet: 0.484 ± 0.026
0.837HisAsn: 0.837 ± 0.035
1.633HisPro: 1.633 ± 0.051
1.635HisGln: 1.635 ± 0.053
1.503HisArg: 1.503 ± 0.043
1.386HisSer: 1.386 ± 0.044
1.293HisThr: 1.293 ± 0.048
1.18HisVal: 1.18 ± 0.045
0.562HisTrp: 0.562 ± 0.029
0.979HisTyr: 0.979 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
4.973IleAla: 4.973 ± 0.092
0.357IleCys: 0.357 ± 0.023
3.913IleAsp: 3.913 ± 0.075
4.525IleGlu: 4.525 ± 0.085
1.626IlePhe: 1.626 ± 0.046
4.04IleGly: 4.04 ± 0.082
1.34IleHis: 1.34 ± 0.043
2.377IleIle: 2.377 ± 0.071
2.453IleLys: 2.453 ± 0.055
4.503IleLeu: 4.503 ± 0.087
1.125IleMet: 1.125 ± 0.046
2.082IleAsn: 2.082 ± 0.062
2.12IlePro: 2.12 ± 0.056
2.476IleGln: 2.476 ± 0.072
2.853IleArg: 2.853 ± 0.071
2.879IleSer: 2.879 ± 0.066
2.853IleThr: 2.853 ± 0.068
3.229IleVal: 3.229 ± 0.082
0.655IleTrp: 0.655 ± 0.031
1.179IleTyr: 1.179 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
4.845LysAla: 4.845 ± 0.091
0.214LysCys: 0.214 ± 0.022
2.488LysAsp: 2.488 ± 0.059
2.555LysGlu: 2.555 ± 0.063
1.032LysPhe: 1.032 ± 0.042
3.014LysGly: 3.014 ± 0.076
1.099LysHis: 1.099 ± 0.042
2.266LysIle: 2.266 ± 0.061
2.561LysLys: 2.561 ± 0.081
4.121LysLeu: 4.121 ± 0.093
1.215LysMet: 1.215 ± 0.044
1.671LysAsn: 1.671 ± 0.054
2.324LysPro: 2.324 ± 0.079
2.603LysGln: 2.603 ± 0.075
2.854LysArg: 2.854 ± 0.069
2.363LysSer: 2.363 ± 0.065
2.982LysThr: 2.982 ± 0.066
3.026LysVal: 3.026 ± 0.083
0.504LysTrp: 0.504 ± 0.027
0.866LysTyr: 0.866 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
10.861LeuAla: 10.861 ± 0.176
0.75LeuCys: 0.75 ± 0.034
6.099LeuAsp: 6.099 ± 0.115
6.064LeuGlu: 6.064 ± 0.102
3.825LeuPhe: 3.825 ± 0.089
7.297LeuGly: 7.297 ± 0.128
2.234LeuHis: 2.234 ± 0.061
5.82LeuIle: 5.82 ± 0.107
5.762LeuLys: 5.762 ± 0.094
10.101LeuLeu: 10.101 ± 0.189
3.071LeuMet: 3.071 ± 0.069
4.326LeuAsn: 4.326 ± 0.086
5.386LeuPro: 5.386 ± 0.108
4.665LeuGln: 4.665 ± 0.104
4.873LeuArg: 4.873 ± 0.085
7.856LeuSer: 7.856 ± 0.112
6.637LeuThr: 6.637 ± 0.105
7.06LeuVal: 7.06 ± 0.12
1.399LeuTrp: 1.399 ± 0.053
2.655LeuTyr: 2.655 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
3.104MetAla: 3.104 ± 0.069
0.124MetCys: 0.124 ± 0.016
1.603MetAsp: 1.603 ± 0.053
1.363MetGlu: 1.363 ± 0.046
0.711MetPhe: 0.711 ± 0.027
1.92MetGly: 1.92 ± 0.064
0.637MetHis: 0.637 ± 0.029
1.472MetIle: 1.472 ± 0.049
1.155MetLys: 1.155 ± 0.042
2.534MetLeu: 2.534 ± 0.062
0.905MetMet: 0.905 ± 0.038
1.04MetAsn: 1.04 ± 0.043
1.456MetPro: 1.456 ± 0.045
1.552MetGln: 1.552 ± 0.051
1.356MetArg: 1.356 ± 0.049
1.854MetSer: 1.854 ± 0.055
1.959MetThr: 1.959 ± 0.056
1.879MetVal: 1.879 ± 0.054
0.182MetTrp: 0.182 ± 0.015
0.43MetTyr: 0.43 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.579AsnAla: 3.579 ± 0.079
0.219AsnCys: 0.219 ± 0.02
2.066AsnAsp: 2.066 ± 0.058
2.033AsnGlu: 2.033 ± 0.062
1.106AsnPhe: 1.106 ± 0.048
2.395AsnGly: 2.395 ± 0.065
0.916AsnHis: 0.916 ± 0.039
1.741AsnIle: 1.741 ± 0.058
1.366AsnLys: 1.366 ± 0.047
3.441AsnLeu: 3.441 ± 0.063
0.814AsnMet: 0.814 ± 0.036
1.141AsnAsn: 1.141 ± 0.049
1.939AsnPro: 1.939 ± 0.056
2.209AsnGln: 2.209 ± 0.061
2.141AsnArg: 2.141 ± 0.051
1.444AsnSer: 1.444 ± 0.051
1.737AsnThr: 1.737 ± 0.06
2.155AsnVal: 2.155 ± 0.068
0.529AsnTrp: 0.529 ± 0.027
0.843AsnTyr: 0.843 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
3.87ProAla: 3.87 ± 0.073
0.326ProCys: 0.326 ± 0.025
3.382ProAsp: 3.382 ± 0.077
3.823ProGlu: 3.823 ± 0.077
1.84ProPhe: 1.84 ± 0.05
3.35ProGly: 3.35 ± 0.072
1.051ProHis: 1.051 ± 0.045
2.331ProIle: 2.331 ± 0.065
2.109ProLys: 2.109 ± 0.064
4.368ProLeu: 4.368 ± 0.087
1.26ProMet: 1.26 ± 0.045
1.632ProAsn: 1.632 ± 0.052
1.779ProPro: 1.779 ± 0.057
2.119ProGln: 2.119 ± 0.057
1.726ProArg: 1.726 ± 0.053
2.62ProSer: 2.62 ± 0.067
2.157ProThr: 2.157 ± 0.062
3.698ProVal: 3.698 ± 0.072
0.639ProTrp: 0.639 ± 0.034
1.282ProTyr: 1.282 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
7.693GlnAla: 7.693 ± 0.126
0.429GlnCys: 0.429 ± 0.025
2.344GlnAsp: 2.344 ± 0.059
2.422GlnGlu: 2.422 ± 0.058
1.646GlnPhe: 1.646 ± 0.048
3.653GlnGly: 3.653 ± 0.079
1.221GlnHis: 1.221 ± 0.04
2.579GlnIle: 2.579 ± 0.056
2.601GlnLys: 2.601 ± 0.066
5.319GlnLeu: 5.319 ± 0.108
1.32GlnMet: 1.32 ± 0.049
1.854GlnAsn: 1.854 ± 0.056
2.654GlnPro: 2.654 ± 0.068
3.031GlnGln: 3.031 ± 0.085
3.276GlnArg: 3.276 ± 0.079
3.807GlnSer: 3.807 ± 0.087
3.985GlnThr: 3.985 ± 0.086
3.781GlnVal: 3.781 ± 0.069
0.968GlnTrp: 0.968 ± 0.045
1.174GlnTyr: 1.174 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
4.317ArgAla: 4.317 ± 0.098
0.443ArgCys: 0.443 ± 0.028
2.89ArgAsp: 2.89 ± 0.072
3.098ArgGlu: 3.098 ± 0.064
2.815ArgPhe: 2.815 ± 0.062
2.809ArgGly: 2.809 ± 0.065
1.681ArgHis: 1.681 ± 0.059
3.082ArgIle: 3.082 ± 0.07
2.203ArgLys: 2.203 ± 0.062
6.926ArgLeu: 6.926 ± 0.13
1.45ArgMet: 1.45 ± 0.046
1.683ArgAsn: 1.683 ± 0.052
2.45ArgPro: 2.45 ± 0.074
3.22ArgGln: 3.22 ± 0.084
3.02ArgArg: 3.02 ± 0.079
2.716ArgSer: 2.716 ± 0.072
2.401ArgThr: 2.401 ± 0.064
3.732ArgVal: 3.732 ± 0.078
0.905ArgTrp: 0.905 ± 0.039
1.825ArgTyr: 1.825 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.217SerAla: 5.217 ± 0.101
0.378SerCys: 0.378 ± 0.024
3.775SerAsp: 3.775 ± 0.087
3.948SerGlu: 3.948 ± 0.084
2.145SerPhe: 2.145 ± 0.054
4.644SerGly: 4.644 ± 0.084
1.564SerHis: 1.564 ± 0.054
2.873SerIle: 2.873 ± 0.074
2.12SerLys: 2.12 ± 0.058
6.388SerLeu: 6.388 ± 0.113
1.626SerMet: 1.626 ± 0.063
1.844SerAsn: 1.844 ± 0.058
2.609SerPro: 2.609 ± 0.06
3.262SerGln: 3.262 ± 0.078
2.811SerArg: 2.811 ± 0.07
3.14SerSer: 3.14 ± 0.076
2.546SerThr: 2.546 ± 0.068
4.393SerVal: 4.393 ± 0.08
0.667SerTrp: 0.667 ± 0.032
1.436SerTyr: 1.436 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
4.566ThrAla: 4.566 ± 0.09
0.408ThrCys: 0.408 ± 0.026
3.459ThrAsp: 3.459 ± 0.072
3.244ThrGlu: 3.244 ± 0.067
2.117ThrPhe: 2.117 ± 0.055
4.075ThrGly: 4.075 ± 0.087
1.585ThrHis: 1.585 ± 0.054
2.463ThrIle: 2.463 ± 0.061
1.593ThrLys: 1.593 ± 0.053
7.439ThrLeu: 7.439 ± 0.106
1.107ThrMet: 1.107 ± 0.044
1.552ThrAsn: 1.552 ± 0.047
3.305ThrPro: 3.305 ± 0.064
3.382ThrGln: 3.382 ± 0.074
2.905ThrArg: 2.905 ± 0.073
2.643ThrSer: 2.643 ± 0.061
2.706ThrThr: 2.706 ± 0.074
3.802ThrVal: 3.802 ± 0.095
0.652ThrTrp: 0.652 ± 0.032
1.511ThrTyr: 1.511 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
6.558ValAla: 6.558 ± 0.116
0.676ValCys: 0.676 ± 0.034
4.057ValAsp: 4.057 ± 0.086
4.264ValGlu: 4.264 ± 0.08
2.825ValPhe: 2.825 ± 0.074
4.512ValGly: 4.512 ± 0.103
1.497ValHis: 1.497 ± 0.048
4.179ValIle: 4.179 ± 0.084
3.14ValLys: 3.14 ± 0.081
6.911ValLeu: 6.911 ± 0.127
2.191ValMet: 2.191 ± 0.06
2.391ValAsn: 2.391 ± 0.053
2.774ValPro: 2.774 ± 0.077
2.728ValGln: 2.728 ± 0.071
3.584ValArg: 3.584 ± 0.079
4.642ValSer: 4.642 ± 0.093
4.255ValThr: 4.255 ± 0.078
5.266ValVal: 5.266 ± 0.107
0.953ValTrp: 0.953 ± 0.042
1.802ValTyr: 1.802 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
1.113TrpAla: 1.113 ± 0.048
0.14TrpCys: 0.14 ± 0.012
0.578TrpAsp: 0.578 ± 0.033
0.67TrpGlu: 0.67 ± 0.029
0.635TrpPhe: 0.635 ± 0.031
0.852TrpGly: 0.852 ± 0.042
0.469TrpHis: 0.469 ± 0.026
0.607TrpIle: 0.607 ± 0.032
0.376TrpLys: 0.376 ± 0.021
2.425TrpLeu: 2.425 ± 0.084
0.41TrpMet: 0.41 ± 0.024
0.328TrpAsn: 0.328 ± 0.027
0.731TrpPro: 0.731 ± 0.038
1.353TrpGln: 1.353 ± 0.05
0.85TrpArg: 0.85 ± 0.034
0.837TrpSer: 0.837 ± 0.042
0.565TrpThr: 0.565 ± 0.031
1.322TrpVal: 1.322 ± 0.048
0.27TrpTrp: 0.27 ± 0.024
0.339TrpTyr: 0.339 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.055
0.259TyrCys: 0.259 ± 0.021
1.831TyrAsp: 1.831 ± 0.051
1.837TyrGlu: 1.837 ± 0.052
1.193TyrPhe: 1.193 ± 0.043
2.014TyrGly: 2.014 ± 0.05
0.788TyrHis: 0.788 ± 0.035
1.068TyrIle: 1.068 ± 0.043
0.963TyrLys: 0.963 ± 0.042
3.01TyrLeu: 3.01 ± 0.069
0.545TyrMet: 0.545 ± 0.027
0.882TyrAsn: 0.882 ± 0.039
1.267TyrPro: 1.267 ± 0.043
2.061TyrGln: 2.061 ± 0.058
1.735TyrArg: 1.735 ± 0.049
1.33TyrSer: 1.33 ± 0.048
1.213TyrThr: 1.213 ± 0.039
1.648TyrVal: 1.648 ± 0.055
0.407TyrTrp: 0.407 ± 0.027
0.902TyrTyr: 0.902 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2043 proteins (688134 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski