Brachymonas denitrificans DSM 15123

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Brachymonas; Brachymonas denitrificans

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2470 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8GWY2|A0A1H8GWY2_9BURK Thioredoxin OS=Brachymonas denitrificans DSM 15123 OX=1121117 GN=SAMN02745977_01363 PE=3 SV=1
MM1 pKa = 6.29TTVAEE6 pKa = 4.27STAAEE11 pKa = 3.99NAAPVSDD18 pKa = 4.72DD19 pKa = 4.66PILFTDD25 pKa = 3.95SAAAKK30 pKa = 10.02VADD33 pKa = 5.45LIAEE37 pKa = 4.31EE38 pKa = 4.82GNPDD42 pKa = 2.99LKK44 pKa = 11.13LRR46 pKa = 11.84VFVQGGGCSGFQYY59 pKa = 10.94GFTFDD64 pKa = 5.34EE65 pKa = 4.5ITNDD69 pKa = 3.87DD70 pKa = 4.1DD71 pKa = 4.78TVMTKK76 pKa = 10.67NGVSLLIDD84 pKa = 3.54AMSYY88 pKa = 10.32QYY90 pKa = 11.5LVGAEE95 pKa = 3.79IDD97 pKa = 3.91YY98 pKa = 11.36KK99 pKa = 11.19EE100 pKa = 4.67DD101 pKa = 3.23LQGAQFVIRR110 pKa = 11.84NPNAEE115 pKa = 4.36TTCGCGSSFSMM126 pKa = 5.09

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8CUR7|A0A1H8CUR7_9BURK SurA N-terminal domain-containing protein OS=Brachymonas denitrificans DSM 15123 OX=1121117 GN=SAMN02745977_00060 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.4GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2470

0

2470

808793

40

2866

327.4

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.141 ± 0.073

0.959 ± 0.015

5.159 ± 0.035

5.493 ± 0.045

3.389 ± 0.03

8.143 ± 0.047

2.384 ± 0.027

4.435 ± 0.039

3.471 ± 0.048

10.756 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.024

2.737 ± 0.03

5.287 ± 0.037

4.707 ± 0.038

6.513 ± 0.037

5.278 ± 0.032

5.064 ± 0.027

7.506 ± 0.036

1.546 ± 0.026

2.3 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski