archaeon SCG-AAA382B04
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1591 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R6T684|A0A2R6T684_9ARCH 7 8-didemethyl-8-hydroxy-5-deazariboflavin synthase OS=archaeon SCG-AAA382B04 OX=2137776 GN=C9439_05135 PE=3 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.41 KK3 pKa = 10.56 EE4 pKa = 4.35 LMDD7 pKa = 4.2 KK8 pKa = 10.24 LACPEE13 pKa = 4.12 CKK15 pKa = 10.17 GDD17 pKa = 3.54 FEE19 pKa = 4.97 LRR21 pKa = 11.84 EE22 pKa = 4.08 EE23 pKa = 4.36 VEE25 pKa = 4.17 DD26 pKa = 4.56 DD27 pKa = 4.21 EE28 pKa = 5.86 IISGEE33 pKa = 4.39 LYY35 pKa = 10.69 CGEE38 pKa = 4.53 CDD40 pKa = 3.0 EE41 pKa = 5.22 TYY43 pKa = 10.21 PIEE46 pKa = 5.37 DD47 pKa = 5.39 GIPNLLPPDD56 pKa = 3.85 FEE58 pKa = 4.51 EE59 pKa = 4.46
Molecular weight: 6.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.821
IPC_protein 3.719
Toseland 3.554
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.91
Wikipedia 3.592
Rodwell 3.567
Grimsley 3.465
Solomon 3.668
Lehninger 3.617
Nozaki 3.821
DTASelect 3.935
Thurlkill 3.592
EMBOSS 3.605
Sillero 3.834
Patrickios 1.825
IPC_peptide 3.668
IPC2_peptide 3.821
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A2R6T6A4|A0A2R6T6A4_9ARCH OrfB_Zn_ribbon domain-containing protein OS=archaeon SCG-AAA382B04 OX=2137776 GN=C9439_04525 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 9.47 MPRR5 pKa = 11.84 RR6 pKa = 11.84 IRR8 pKa = 11.84 THH10 pKa = 6.62 CPFCNEE16 pKa = 4.04 HH17 pKa = 6.37 NEE19 pKa = 4.18 HH20 pKa = 7.15 EE21 pKa = 4.21 IQKK24 pKa = 10.0 ARR26 pKa = 11.84 KK27 pKa = 7.86 GQPSEE32 pKa = 4.21 MKK34 pKa = 9.11 WGQRR38 pKa = 11.84 QFRR41 pKa = 11.84 RR42 pKa = 11.84 VTSGYY47 pKa = 9.73 RR48 pKa = 11.84 GYY50 pKa = 9.97 PRR52 pKa = 11.84 PLPPGGKK59 pKa = 7.55 PTQKK63 pKa = 9.7 VDD65 pKa = 2.97 LRR67 pKa = 11.84 YY68 pKa = 10.26 RR69 pKa = 11.84 CTEE72 pKa = 3.7 CGKK75 pKa = 9.9 AHH77 pKa = 5.34 TRR79 pKa = 11.84 EE80 pKa = 4.0 GFRR83 pKa = 11.84 VSRR86 pKa = 11.84 LEE88 pKa = 4.25 LEE90 pKa = 4.51 GG91 pKa = 4.11
Molecular weight: 10.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.545
IPC_protein 10.087
Toseland 10.54
ProMoST 10.175
Dawson 10.643
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 10.921
Grimsley 10.687
Solomon 10.73
Lehninger 10.701
Nozaki 10.555
DTASelect 10.321
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.584
Patrickios 10.672
IPC_peptide 10.745
IPC2_peptide 9.502
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1591
0
1591
408258
21
1938
256.6
29.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.279 ± 0.067
0.9 ± 0.027
5.726 ± 0.054
9.521 ± 0.094
4.038 ± 0.06
6.724 ± 0.055
1.559 ± 0.027
8.378 ± 0.075
9.546 ± 0.117
9.148 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.965 ± 0.03
5.029 ± 0.057
3.544 ± 0.037
2.802 ± 0.038
4.354 ± 0.051
6.307 ± 0.052
4.863 ± 0.071
6.236 ± 0.05
0.861 ± 0.021
3.22 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here