Gordonia phage Phistory

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Langleyhallvirinae; Phistoryvirus; Gordonia virus Phistory

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385E1M0|A0A385E1M0_9CAUD Uncharacterized protein OS=Gordonia phage Phistory OX=2301694 GN=30 PE=4 SV=1
MM1 pKa = 6.8TATRR5 pKa = 11.84FSFYY9 pKa = 11.1AEE11 pKa = 4.11PAPVATRR18 pKa = 11.84NDD20 pKa = 3.43DD21 pKa = 3.75CYY23 pKa = 11.57VGVGEE28 pKa = 4.68CTDD31 pKa = 3.43GAEE34 pKa = 4.8AIAIVTPGDD43 pKa = 3.24LHH45 pKa = 7.3LSHH48 pKa = 6.55EE49 pKa = 4.34QARR52 pKa = 11.84ALVAEE57 pKa = 4.99LSSAITVLSEE67 pKa = 3.67GAEE70 pKa = 4.03AA71 pKa = 5.1

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E1Q1|A0A385E1Q1_9CAUD Uncharacterized protein OS=Gordonia phage Phistory OX=2301694 GN=87 PE=4 SV=1
MM1 pKa = 7.24IGKK4 pKa = 8.92PIRR7 pKa = 11.84EE8 pKa = 4.13LVATEE13 pKa = 4.45DD14 pKa = 3.95DD15 pKa = 4.15PLFGLSARR23 pKa = 11.84YY24 pKa = 9.22VRR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 5.75LEE30 pKa = 3.78AGGLFRR36 pKa = 11.84GLGSQRR42 pKa = 11.84CKK44 pKa = 10.29RR45 pKa = 11.84GEE47 pKa = 3.73WLLNRR52 pKa = 11.84EE53 pKa = 4.45DD54 pKa = 3.27VAEE57 pKa = 3.93IQRR60 pKa = 11.84RR61 pKa = 11.84LRR63 pKa = 11.84AVPNEE68 pKa = 4.07EE69 pKa = 4.08PVEE72 pKa = 4.12LDD74 pKa = 3.51TPSGLSSRR82 pKa = 11.84SRR84 pKa = 11.84TLRR87 pKa = 11.84RR88 pKa = 11.84IQQGRR93 pKa = 11.84AASS96 pKa = 3.46

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

16934

32

1875

159.8

17.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.954 ± 0.431

1.24 ± 0.241

6.407 ± 0.321

5.917 ± 0.343

2.622 ± 0.211

8.326 ± 0.442

2.297 ± 0.167

4.724 ± 0.187

3.649 ± 0.296

7.736 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.146

2.775 ± 0.204

5.338 ± 0.195

3.484 ± 0.177

7.435 ± 0.441

5.988 ± 0.276

6.519 ± 0.309

7.588 ± 0.361

2.35 ± 0.135

2.291 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski