Idiomarina aquatica
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2765 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6PKT2|A0A4R6PKT2_9GAMM Release factor glutamine methyltransferase OS=Idiomarina aquatica OX=1327752 GN=prmC PE=3 SV=1
MM1 pKa = 7.52 SLQIGLFYY9 pKa = 11.09 GSTTCYY15 pKa = 10.5 TEE17 pKa = 3.5 IAAEE21 pKa = 4.65 KK22 pKa = 8.43 IQQAIGEE29 pKa = 4.98 DD30 pKa = 3.55 IVSLHH35 pKa = 6.38 NIKK38 pKa = 9.24 DD39 pKa = 3.71 TPLAEE44 pKa = 4.36 AEE46 pKa = 4.39 QYY48 pKa = 10.96 DD49 pKa = 3.73 ILIFGISTWDD59 pKa = 3.43 FGEE62 pKa = 4.3 LQEE65 pKa = 5.29 DD66 pKa = 4.06 WEE68 pKa = 5.13 SYY70 pKa = 9.84 WDD72 pKa = 6.11 DD73 pKa = 3.42 ITPLNLDD80 pKa = 3.54 GKK82 pKa = 10.26 IVALYY87 pKa = 11.02 GMGDD91 pKa = 3.41 QEE93 pKa = 6.47 GYY95 pKa = 9.41 TDD97 pKa = 3.85 WFQDD101 pKa = 3.72 ALGMLHH107 pKa = 7.46 DD108 pKa = 6.15 AIADD112 pKa = 3.98 KK113 pKa = 10.65 NCKK116 pKa = 10.11 RR117 pKa = 11.84 IGFWPNQGYY126 pKa = 10.1 DD127 pKa = 3.76 FAASKK132 pKa = 10.94 ALTADD137 pKa = 3.07 QSQFVGLSLDD147 pKa = 3.62 EE148 pKa = 4.2 DD149 pKa = 4.07 TQYY152 pKa = 12.01 DD153 pKa = 3.86 LTDD156 pKa = 3.29 EE157 pKa = 5.84 RR158 pKa = 11.84 IEE160 pKa = 3.92 QWTTQVLEE168 pKa = 5.03 EE169 pKa = 4.4 IAEE172 pKa = 4.13 QLL174 pKa = 3.63
Molecular weight: 19.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.821
Patrickios 1.036
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A4R6NYU2|A0A4R6NYU2_9GAMM Diguanylate cyclase/phosphodiesterase OS=Idiomarina aquatica OX=1327752 GN=DEU29_12522 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 KK29 pKa = 9.36 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.91 VLSAA44 pKa = 4.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2765
0
2765
909821
27
1614
329.0
36.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.351 ± 0.051
0.87 ± 0.015
5.906 ± 0.037
6.335 ± 0.043
3.884 ± 0.033
6.837 ± 0.046
2.214 ± 0.025
5.69 ± 0.033
4.541 ± 0.039
10.432 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.433 ± 0.025
3.924 ± 0.03
4.06 ± 0.027
5.402 ± 0.056
5.391 ± 0.039
6.16 ± 0.039
5.206 ± 0.033
7.041 ± 0.034
1.361 ± 0.02
2.962 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here