Streptococcus phage Javan83
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ARQ2|A0A4D6ARQ2_9CAUD Essential recombination function protein OS=Streptococcus phage Javan83 OX=2548308 GN=Javan83_0008 PE=4 SV=1
MM1 pKa = 7.51 NKK3 pKa = 9.68 NDD5 pKa = 4.12 LFVDD9 pKa = 4.34 VASHH13 pKa = 5.34 QGYY16 pKa = 10.07 NITGILGQMGTTNTIIKK33 pKa = 9.7 ISEE36 pKa = 3.99 STSYY40 pKa = 11.02 INPCLSAQVEE50 pKa = 4.11 QSNPIGFYY58 pKa = 9.88 HH59 pKa = 5.76 YY60 pKa = 10.64 AWFGGDD66 pKa = 2.96 VAEE69 pKa = 4.77 AEE71 pKa = 3.99 RR72 pKa = 11.84 EE73 pKa = 3.78 ARR75 pKa = 11.84 YY76 pKa = 9.49 FIDD79 pKa = 4.55 NVPQKK84 pKa = 11.01 VKK86 pKa = 10.38 YY87 pKa = 10.42 LCLDD91 pKa = 3.96 YY92 pKa = 10.92 EE93 pKa = 4.44 DD94 pKa = 5.0 HH95 pKa = 7.98 ASGDD99 pKa = 3.59 KK100 pKa = 10.11 QANTDD105 pKa = 2.83 ACLRR109 pKa = 11.84 FMEE112 pKa = 4.24 ILKK115 pKa = 10.54 EE116 pKa = 3.95 NGYY119 pKa = 10.25 EE120 pKa = 4.01 PIYY123 pKa = 10.7 YY124 pKa = 9.76 SYY126 pKa = 11.64 KK127 pKa = 10.54 PFTLNNIYY135 pKa = 10.45 YY136 pKa = 8.43 EE137 pKa = 4.23 QILARR142 pKa = 11.84 FPNSLWIAGYY152 pKa = 10.38 GLNDD156 pKa = 3.33 GNADD160 pKa = 3.61 FNYY163 pKa = 8.87 FPSMDD168 pKa = 4.52 GIRR171 pKa = 11.84 WWQYY175 pKa = 10.03 SSNPFDD181 pKa = 4.42 KK182 pKa = 11.23 NIVLLDD188 pKa = 3.94 DD189 pKa = 4.18 EE190 pKa = 5.68 EE191 pKa = 4.64 EE192 pKa = 4.62 DD193 pKa = 4.11 NVISKK198 pKa = 10.66 DD199 pKa = 3.49 AVKK202 pKa = 10.71 SLNTVADD209 pKa = 3.93 EE210 pKa = 4.71 VIQGLWGNGQEE221 pKa = 5.08 RR222 pKa = 11.84 FDD224 pKa = 3.84 NLTNAGYY231 pKa = 9.79 NAQAVQDD238 pKa = 4.13 KK239 pKa = 11.44 VNDD242 pKa = 3.94 LLNAEE247 pKa = 4.45 NTSKK251 pKa = 11.19 DD252 pKa = 3.75 LDD254 pKa = 3.81 TLANEE259 pKa = 4.39 VLQGLWGNGQEE270 pKa = 5.14 RR271 pKa = 11.84 FDD273 pKa = 3.71 KK274 pKa = 11.2 LEE276 pKa = 3.98 NAGYY280 pKa = 9.79 DD281 pKa = 3.43 AQAVQDD287 pKa = 4.1 RR288 pKa = 11.84 VNSLLSGGNTADD300 pKa = 4.36 LDD302 pKa = 4.12 TVANEE307 pKa = 4.18 VVQGLWGNGQEE318 pKa = 5.08 RR319 pKa = 11.84 FDD321 pKa = 3.84 NLTNAGYY328 pKa = 9.89 DD329 pKa = 3.29 AQAVQNRR336 pKa = 11.84 VNEE339 pKa = 4.16 LLSS342 pKa = 3.58
Molecular weight: 38.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.023
IPC2_protein 4.126
IPC_protein 4.126
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.999
Rodwell 3.948
Grimsley 3.821
Solomon 4.088
Lehninger 4.05
Nozaki 4.202
DTASelect 4.418
Thurlkill 3.948
EMBOSS 4.012
Sillero 4.24
Patrickios 2.816
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.147
Protein with the highest isoelectric point:
>tr|A0A4D6APD7|A0A4D6APD7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan83 OX=2548308 GN=Javan83_0052 PE=4 SV=1
MM1 pKa = 7.72 ADD3 pKa = 3.16 VRR5 pKa = 11.84 VSIDD9 pKa = 3.26 LAGVEE14 pKa = 4.86 KK15 pKa = 10.52 KK16 pKa = 10.72 VSPQAMQRR24 pKa = 11.84 GKK26 pKa = 9.98 IAAGSEE32 pKa = 3.87 ALLIMDD38 pKa = 4.07 SSVPLRR44 pKa = 11.84 AGGGALRR51 pKa = 11.84 ASGRR55 pKa = 11.84 VEE57 pKa = 3.73 PNGNVSYY64 pKa = 9.14 NTVYY68 pKa = 10.96 ARR70 pKa = 11.84 AQFHH74 pKa = 5.45 GTNGIVVFRR83 pKa = 11.84 KK84 pKa = 8.49 YY85 pKa = 6.16 TTSGTGSRR93 pKa = 11.84 WDD95 pKa = 3.86 KK96 pKa = 10.38 PLKK99 pKa = 10.93 ANIDD103 pKa = 3.85 KK104 pKa = 10.78 LKK106 pKa = 10.34 KK107 pKa = 10.11 AAIKK111 pKa = 10.82 GMGIRR116 pKa = 4.21
Molecular weight: 12.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 9.765
IPC_protein 10.189
Toseland 10.76
ProMoST 10.511
Dawson 10.847
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.286
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.482
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.76
Patrickios 11.023
IPC_peptide 10.921
IPC2_peptide 9.019
IPC2.peptide.svr19 8.733
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
11218
48
1293
211.7
23.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.247 ± 0.981
0.526 ± 0.108
6.276 ± 0.452
6.864 ± 0.552
4.172 ± 0.23
6.605 ± 0.596
1.373 ± 0.15
7.31 ± 0.298
8.121 ± 0.452
8.388 ± 0.269
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.469 ± 0.246
6.106 ± 0.327
2.549 ± 0.206
4.511 ± 0.224
3.601 ± 0.339
6.4 ± 0.464
5.999 ± 0.233
6.463 ± 0.239
1.007 ± 0.14
4.011 ± 0.42
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here