Groundnut chlorotic fan-spot virus
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D4D8M7|A0A0D4D8M7_9VIRU Envelopment polyprotein OS=Groundnut chlorotic fan-spot virus OX=1629551 PE=4 SV=1
MM1 pKa = 7.42 SSSLPIYY8 pKa = 10.14 LSGFIFYY15 pKa = 8.12 VTSLLVIMILMLCLILNLRR34 pKa = 11.84 QLSSSEE40 pKa = 4.03 KK41 pKa = 10.05 SPSLEE46 pKa = 3.96 SQSGKK51 pKa = 10.31 KK52 pKa = 9.76 KK53 pKa = 10.23 YY54 pKa = 10.05 SGTYY58 pKa = 9.1 RR59 pKa = 11.84 SVMLSDD65 pKa = 4.28 SYY67 pKa = 11.42 TDD69 pKa = 3.71 KK70 pKa = 11.02 EE71 pKa = 4.01 KK72 pKa = 10.93 CRR74 pKa = 11.84 YY75 pKa = 7.93 TEE77 pKa = 4.13 QNHH80 pKa = 5.58 EE81 pKa = 4.34 PPLPSNCMIVGKK93 pKa = 8.23 STFNFLATMNEE104 pKa = 3.46 GDD106 pKa = 5.29 DD107 pKa = 4.92 YY108 pKa = 10.43 YY109 pKa = 10.89 TCPSEE114 pKa = 5.09 NVTFYY119 pKa = 11.13 KK120 pKa = 10.1 KK121 pKa = 10.48 CKK123 pKa = 10.08 NINLLSDD130 pKa = 4.38 FNSEE134 pKa = 3.25 ITIPVVPVMNVEE146 pKa = 4.14 NKK148 pKa = 10.05 RR149 pKa = 11.84 LLEE152 pKa = 4.23 VGSKK156 pKa = 10.43 YY157 pKa = 10.5 FEE159 pKa = 4.06 EE160 pKa = 5.53 KK161 pKa = 10.16 IGSKK165 pKa = 7.26 QHH167 pKa = 6.01 IVLHH171 pKa = 5.18 SVKK174 pKa = 10.65 LSGDD178 pKa = 3.82 CNISQLNSDD187 pKa = 3.37 EE188 pKa = 4.35 TFNVNLEE195 pKa = 4.3 TTEE198 pKa = 3.95 KK199 pKa = 10.85 LIGVTVKK206 pKa = 10.63 QMGQLDD212 pKa = 4.04 SKK214 pKa = 10.31 VQSFTGTFQYY224 pKa = 10.83 QLSEE228 pKa = 4.26 DD229 pKa = 4.01 SLDD232 pKa = 3.53 GFHH235 pKa = 7.21 YY236 pKa = 9.39 LICGDD241 pKa = 4.42 KK242 pKa = 10.48 FFHH245 pKa = 6.88 LPNVDD250 pKa = 3.14 NGLRR254 pKa = 11.84 RR255 pKa = 11.84 CVHH258 pKa = 7.01 KK259 pKa = 11.21 YY260 pKa = 9.73 QDD262 pKa = 4.21 NKK264 pKa = 9.93 TMLYY268 pKa = 9.01 TCVNFYY274 pKa = 8.39 WLKK277 pKa = 8.82 WAFLFFLIMMPITWIMNKK295 pKa = 8.77 TKK297 pKa = 10.69 GPLSLWYY304 pKa = 9.74 DD305 pKa = 3.75 LLSIVLYY312 pKa = 9.51 PLIYY316 pKa = 10.55 LLNNLWSKK324 pKa = 9.82 MPYY327 pKa = 9.1 RR328 pKa = 11.84 CDD330 pKa = 2.98 MCGYY334 pKa = 10.67 LSLLSHH340 pKa = 7.47 DD341 pKa = 4.91 CPPKK345 pKa = 10.07 CVCGRR350 pKa = 11.84 SDD352 pKa = 4.59 SGALHH357 pKa = 6.99 NPDD360 pKa = 4.35 CYY362 pKa = 10.83 FNRR365 pKa = 11.84 GFPKK369 pKa = 10.39 KK370 pKa = 9.63 PSKK373 pKa = 10.57 KK374 pKa = 10.07 FQFLMNTGITRR385 pKa = 11.84 NVTFYY390 pKa = 10.04 LTRR393 pKa = 11.84 FMLSTIVISILPNTLALQASCGYY416 pKa = 10.08 NCYY419 pKa = 10.82 ADD421 pKa = 5.08 LEE423 pKa = 4.42 QSKK426 pKa = 10.0 FLYY429 pKa = 9.98 IGSHH433 pKa = 5.36 SMPSARR439 pKa = 11.84 EE440 pKa = 3.91 CDD442 pKa = 3.59 CKK444 pKa = 10.01 ITKK447 pKa = 10.33 DD448 pKa = 3.55 SGSLDD453 pKa = 3.53 FPFKK457 pKa = 10.85 VVEE460 pKa = 4.43 TYY462 pKa = 11.29 KK463 pKa = 10.96 DD464 pKa = 3.3 ITGKK468 pKa = 8.02 TQTNIANKK476 pKa = 9.8 KK477 pKa = 9.5 CYY479 pKa = 9.61 PDD481 pKa = 5.04 FDD483 pKa = 5.89 DD484 pKa = 5.96 DD485 pKa = 5.13 CADD488 pKa = 3.77 HH489 pKa = 7.39 LDD491 pKa = 4.0 TEE493 pKa = 4.51 VLRR496 pKa = 11.84 ILACAYY502 pKa = 10.27 SCEE505 pKa = 3.92 ALKK508 pKa = 10.2 MYY510 pKa = 9.94 IYY512 pKa = 10.54 EE513 pKa = 4.46 PLPSVHH519 pKa = 6.58 EE520 pKa = 4.49 SYY522 pKa = 10.68 KK523 pKa = 10.81 GEE525 pKa = 4.38 AYY527 pKa = 10.6 GKK529 pKa = 8.49 MKK531 pKa = 10.47 PIDD534 pKa = 4.32 LYY536 pKa = 11.42 SLQKK540 pKa = 10.69 SRR542 pKa = 11.84 TGVIDD547 pKa = 3.09 NRR549 pKa = 11.84 TILSRR554 pKa = 11.84 QEE556 pKa = 4.0 PSLSTWEE563 pKa = 4.08 KK564 pKa = 9.09 TNSVMSKK571 pKa = 10.62 AIASLDD577 pKa = 3.61 KK578 pKa = 11.07 LDD580 pKa = 3.87 VSSISDD586 pKa = 3.7 SNTLSRR592 pKa = 11.84 TTLMYY597 pKa = 10.41 SSKK600 pKa = 10.69 VDD602 pKa = 3.19 GKK604 pKa = 9.28 YY605 pKa = 10.28 RR606 pKa = 11.84 YY607 pKa = 9.32 MIEE610 pKa = 3.75 QDD612 pKa = 4.5 LISDD616 pKa = 4.15 TGTVFEE622 pKa = 4.85 MVDD625 pKa = 3.24 GTMSHH630 pKa = 6.04 SKK632 pKa = 10.93 KK633 pKa = 10.94 LMITHH638 pKa = 6.74 KK639 pKa = 10.51 NVGVTYY645 pKa = 9.4 TIEE648 pKa = 4.01 YY649 pKa = 9.92 LYY651 pKa = 8.39 TTADD655 pKa = 2.85 IVTTKK660 pKa = 10.25 TSIYY664 pKa = 10.27 SSCTGNCEE672 pKa = 4.16 KK673 pKa = 10.48 CWEE676 pKa = 4.39 GLGSTDD682 pKa = 2.96 NPSYY686 pKa = 11.09 HH687 pKa = 6.68 KK688 pKa = 10.62 FCLEE692 pKa = 3.85 PTSQWGCEE700 pKa = 3.94 DD701 pKa = 3.9 FMCIAMNEE709 pKa = 4.14 GSTCGHH715 pKa = 7.1 CDD717 pKa = 3.06 NVYY720 pKa = 11.44 DD721 pKa = 4.29 MSNSLDD727 pKa = 3.47 VYY729 pKa = 9.88 RR730 pKa = 11.84 VKK732 pKa = 10.43 EE733 pKa = 3.88 VHH735 pKa = 6.32 VSSTACVNAGKK746 pKa = 10.6 GYY748 pKa = 10.65 NCFNHH753 pKa = 7.67 DD754 pKa = 4.28 DD755 pKa = 3.82 RR756 pKa = 11.84 KK757 pKa = 10.95 AYY759 pKa = 9.75 TNDD762 pKa = 3.14 NYY764 pKa = 10.6 QVSITSTLQNDD775 pKa = 4.66 YY776 pKa = 11.0 ISVGHH781 pKa = 6.74 KK782 pKa = 10.06 LALAKK787 pKa = 10.71 DD788 pKa = 4.17 GSAYY792 pKa = 9.42 TGNIADD798 pKa = 4.91 LMSADD803 pKa = 3.69 MTFGHH808 pKa = 6.59 PQLNKK813 pKa = 10.11 QGEE816 pKa = 4.28 LLFAEE821 pKa = 4.93 EE822 pKa = 4.63 YY823 pKa = 9.98 IGKK826 pKa = 8.58 EE827 pKa = 3.75 QLSWDD832 pKa = 4.18 CEE834 pKa = 4.31 FIGPKK839 pKa = 9.53 KK840 pKa = 10.89 VYY842 pKa = 9.54 IKK844 pKa = 10.75 KK845 pKa = 10.26 CGFYY849 pKa = 10.39 TFDD852 pKa = 3.89 HH853 pKa = 6.65 FSGLEE858 pKa = 3.76 KK859 pKa = 10.72 LNEE862 pKa = 4.01 GSFVITGLNKK872 pKa = 8.67 ITMLKK877 pKa = 10.25 DD878 pKa = 3.25 YY879 pKa = 10.91 SLGKK883 pKa = 10.18 IKK885 pKa = 9.72 TVLDD889 pKa = 3.74 LPIEE893 pKa = 4.21 LFVEE897 pKa = 4.4 TTEE900 pKa = 4.08 APKK903 pKa = 11.11 VNIIDD908 pKa = 4.29 HH909 pKa = 6.24 FCSGCLEE916 pKa = 4.28 CFKK919 pKa = 11.28 GLDD922 pKa = 3.66 CKK924 pKa = 11.31 YY925 pKa = 10.44 KK926 pKa = 10.49 INSNKK931 pKa = 9.57 HH932 pKa = 3.82 FASRR936 pKa = 11.84 ISSDD940 pKa = 3.46 FCVSKK945 pKa = 10.24 HH946 pKa = 5.31 QYY948 pKa = 10.65 LIVEE952 pKa = 4.7 RR953 pKa = 11.84 GSNEE957 pKa = 2.98 ISMNLHH963 pKa = 6.33 CGVLPEE969 pKa = 3.99 NAVLKK974 pKa = 10.01 MIVEE978 pKa = 4.44 DD979 pKa = 4.2 NEE981 pKa = 4.52 NISLDD986 pKa = 3.37 IPLTKK991 pKa = 9.67 IEE993 pKa = 4.98 IINDD997 pKa = 3.52 KK998 pKa = 10.83 NVEE1001 pKa = 4.13 TLKK1004 pKa = 10.73 DD1005 pKa = 3.33 HH1006 pKa = 7.68 RR1007 pKa = 11.84 IQKK1010 pKa = 9.67 SFQHH1014 pKa = 6.9 KK1015 pKa = 10.11 EE1016 pKa = 3.67 DD1017 pKa = 3.85 TDD1019 pKa = 3.76 FHH1021 pKa = 6.46 TFLEE1025 pKa = 4.86 TVTYY1029 pKa = 9.07 PFNWVANFTNSALDD1043 pKa = 3.9 LLKK1046 pKa = 10.81 LALLFGFIILGLYY1059 pKa = 9.45 VLSKK1063 pKa = 10.6 CVNLGKK1069 pKa = 9.94 EE1070 pKa = 4.44 AYY1072 pKa = 9.26 SHH1074 pKa = 5.29 ATIYY1078 pKa = 10.27 IKK1080 pKa = 10.53 KK1081 pKa = 9.83 KK1082 pKa = 10.2 KK1083 pKa = 9.85 HH1084 pKa = 6.8 DD1085 pKa = 4.91 DD1086 pKa = 3.56 NDD1088 pKa = 4.23 SDD1090 pKa = 4.21 VDD1092 pKa = 3.76 DD1093 pKa = 4.63 SEE1095 pKa = 4.92 MYY1097 pKa = 10.71 GEE1099 pKa = 4.33 KK1100 pKa = 10.71 NLVRR1104 pKa = 11.84 RR1105 pKa = 11.84 LKK1107 pKa = 11.11 YY1108 pKa = 9.83 IDD1110 pKa = 3.53 LL1111 pKa = 4.45
Molecular weight: 126.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.587
IPC2_protein 5.753
IPC_protein 5.804
Toseland 6.071
ProMoST 6.186
Dawson 6.097
Bjellqvist 6.109
Wikipedia 6.109
Rodwell 6.084
Grimsley 6.16
Solomon 6.097
Lehninger 6.084
Nozaki 6.376
DTASelect 6.547
Thurlkill 6.561
EMBOSS 6.532
Sillero 6.465
Patrickios 3.312
IPC_peptide 6.109
IPC2_peptide 6.415
IPC2.peptide.svr19 6.418
Protein with the highest isoelectric point:
>tr|A0A0D4D8M7|A0A0D4D8M7_9VIRU Envelopment polyprotein OS=Groundnut chlorotic fan-spot virus OX=1629551 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.21 MISRR6 pKa = 11.84 IGSSINSTVTNGLQNLGRR24 pKa = 11.84 TNEE27 pKa = 4.1 DD28 pKa = 3.06 TDD30 pKa = 4.11 VLGSDD35 pKa = 3.37 NRR37 pKa = 11.84 TLMRR41 pKa = 11.84 MPTKK45 pKa = 10.26 RR46 pKa = 11.84 DD47 pKa = 3.14 AEE49 pKa = 4.34 KK50 pKa = 10.85 ARR52 pKa = 11.84 EE53 pKa = 3.81 TRR55 pKa = 11.84 LQGFKK60 pKa = 10.09 EE61 pKa = 4.06 RR62 pKa = 11.84 NVIEE66 pKa = 4.58 SGPSEE71 pKa = 4.0 LGEE74 pKa = 3.88 YY75 pKa = 10.32 DD76 pKa = 3.9 GNPVITSDD84 pKa = 4.21 LSILEE89 pKa = 3.99 RR90 pKa = 11.84 LEE92 pKa = 4.65 VNTSNHH98 pKa = 4.74 ISNWKK103 pKa = 8.3 TDD105 pKa = 3.17 VFLGNGQEE113 pKa = 4.14 MVKK116 pKa = 10.46 KK117 pKa = 10.21 DD118 pKa = 3.31 INLIPTWDD126 pKa = 3.54 SKK128 pKa = 11.32 KK129 pKa = 10.88 EE130 pKa = 3.71 YY131 pKa = 8.67 MQISRR136 pKa = 11.84 VIIWIVPVAPGTKK149 pKa = 10.0 GKK151 pKa = 10.25 IKK153 pKa = 10.51 AALVDD158 pKa = 3.9 RR159 pKa = 11.84 NKK161 pKa = 10.95 AEE163 pKa = 3.9 SEE165 pKa = 3.89 QIIFQKK171 pKa = 10.63 EE172 pKa = 4.09 GVLTDD177 pKa = 3.7 PLCFIFYY184 pKa = 7.23 MHH186 pKa = 7.19 WSYY189 pKa = 11.47 LKK191 pKa = 10.66 SVNNKK196 pKa = 9.12 ALCPQLKK203 pKa = 9.9 FISNEE208 pKa = 3.98 VYY210 pKa = 10.42 KK211 pKa = 11.01 KK212 pKa = 10.27 NVPFAVANFAWRR224 pKa = 11.84 KK225 pKa = 7.36 VFCNSPIAMTEE236 pKa = 3.92 VQPDD240 pKa = 4.58 LIVLNRR246 pKa = 11.84 GITIRR251 pKa = 11.84 NKK253 pKa = 10.69 ALLEE257 pKa = 3.97 AVKK260 pKa = 10.77 CIVPNGNNGKK270 pKa = 7.83 TIKK273 pKa = 10.38 KK274 pKa = 9.99 QIEE277 pKa = 4.37 SLSKK281 pKa = 10.57 SLEE284 pKa = 3.91 AAALEE289 pKa = 4.71 DD290 pKa = 4.07 EE291 pKa = 4.78 SEE293 pKa = 4.36 STGAEE298 pKa = 4.02 MKK300 pKa = 10.59 EE301 pKa = 3.94 LTFDD305 pKa = 3.89 LL306 pKa = 4.9
Molecular weight: 34.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.613
IPC2_protein 6.839
IPC_protein 7.015
Toseland 6.722
ProMoST 7.439
Dawson 7.673
Bjellqvist 7.6
Wikipedia 7.6
Rodwell 7.658
Grimsley 6.722
Solomon 7.79
Lehninger 7.819
Nozaki 7.951
DTASelect 7.746
Thurlkill 7.805
EMBOSS 7.922
Sillero 8.083
Patrickios 4.8
IPC_peptide 7.79
IPC2_peptide 6.942
IPC2.peptide.svr19 7.383
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
4287
306
2870
1429.0
164.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.732 ± 0.393
2.379 ± 0.552
6.181 ± 0.256
6.461 ± 0.503
4.712 ± 0.34
4.129 ± 0.65
2.029 ± 0.31
7.488 ± 0.502
8.771 ± 0.264
9.447 ± 0.302
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.732 ± 0.367
6.765 ± 0.374
2.892 ± 0.269
2.426 ± 0.108
3.569 ± 0.519
9.097 ± 0.312
5.482 ± 0.485
5.365 ± 0.333
0.77 ± 0.204
4.572 ± 0.598
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here