Groundnut chlorotic fan-spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Groundnut chlorotic fan spot orthotospovirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D4D8M7|A0A0D4D8M7_9VIRU Envelopment polyprotein OS=Groundnut chlorotic fan-spot virus OX=1629551 PE=4 SV=1
MM1 pKa = 7.42SSSLPIYY8 pKa = 10.14LSGFIFYY15 pKa = 8.12VTSLLVIMILMLCLILNLRR34 pKa = 11.84QLSSSEE40 pKa = 4.03KK41 pKa = 10.05SPSLEE46 pKa = 3.96SQSGKK51 pKa = 10.31KK52 pKa = 9.76KK53 pKa = 10.23YY54 pKa = 10.05SGTYY58 pKa = 9.1RR59 pKa = 11.84SVMLSDD65 pKa = 4.28SYY67 pKa = 11.42TDD69 pKa = 3.71KK70 pKa = 11.02EE71 pKa = 4.01KK72 pKa = 10.93CRR74 pKa = 11.84YY75 pKa = 7.93TEE77 pKa = 4.13QNHH80 pKa = 5.58EE81 pKa = 4.34PPLPSNCMIVGKK93 pKa = 8.23STFNFLATMNEE104 pKa = 3.46GDD106 pKa = 5.29DD107 pKa = 4.92YY108 pKa = 10.43YY109 pKa = 10.89TCPSEE114 pKa = 5.09NVTFYY119 pKa = 11.13KK120 pKa = 10.1KK121 pKa = 10.48CKK123 pKa = 10.08NINLLSDD130 pKa = 4.38FNSEE134 pKa = 3.25ITIPVVPVMNVEE146 pKa = 4.14NKK148 pKa = 10.05RR149 pKa = 11.84LLEE152 pKa = 4.23VGSKK156 pKa = 10.43YY157 pKa = 10.5FEE159 pKa = 4.06EE160 pKa = 5.53KK161 pKa = 10.16IGSKK165 pKa = 7.26QHH167 pKa = 6.01IVLHH171 pKa = 5.18SVKK174 pKa = 10.65LSGDD178 pKa = 3.82CNISQLNSDD187 pKa = 3.37EE188 pKa = 4.35TFNVNLEE195 pKa = 4.3TTEE198 pKa = 3.95KK199 pKa = 10.85LIGVTVKK206 pKa = 10.63QMGQLDD212 pKa = 4.04SKK214 pKa = 10.31VQSFTGTFQYY224 pKa = 10.83QLSEE228 pKa = 4.26DD229 pKa = 4.01SLDD232 pKa = 3.53GFHH235 pKa = 7.21YY236 pKa = 9.39LICGDD241 pKa = 4.42KK242 pKa = 10.48FFHH245 pKa = 6.88LPNVDD250 pKa = 3.14NGLRR254 pKa = 11.84RR255 pKa = 11.84CVHH258 pKa = 7.01KK259 pKa = 11.21YY260 pKa = 9.73QDD262 pKa = 4.21NKK264 pKa = 9.93TMLYY268 pKa = 9.01TCVNFYY274 pKa = 8.39WLKK277 pKa = 8.82WAFLFFLIMMPITWIMNKK295 pKa = 8.77TKK297 pKa = 10.69GPLSLWYY304 pKa = 9.74DD305 pKa = 3.75LLSIVLYY312 pKa = 9.51PLIYY316 pKa = 10.55LLNNLWSKK324 pKa = 9.82MPYY327 pKa = 9.1RR328 pKa = 11.84CDD330 pKa = 2.98MCGYY334 pKa = 10.67LSLLSHH340 pKa = 7.47DD341 pKa = 4.91CPPKK345 pKa = 10.07CVCGRR350 pKa = 11.84SDD352 pKa = 4.59SGALHH357 pKa = 6.99NPDD360 pKa = 4.35CYY362 pKa = 10.83FNRR365 pKa = 11.84GFPKK369 pKa = 10.39KK370 pKa = 9.63PSKK373 pKa = 10.57KK374 pKa = 10.07FQFLMNTGITRR385 pKa = 11.84NVTFYY390 pKa = 10.04LTRR393 pKa = 11.84FMLSTIVISILPNTLALQASCGYY416 pKa = 10.08NCYY419 pKa = 10.82ADD421 pKa = 5.08LEE423 pKa = 4.42QSKK426 pKa = 10.0FLYY429 pKa = 9.98IGSHH433 pKa = 5.36SMPSARR439 pKa = 11.84EE440 pKa = 3.91CDD442 pKa = 3.59CKK444 pKa = 10.01ITKK447 pKa = 10.33DD448 pKa = 3.55SGSLDD453 pKa = 3.53FPFKK457 pKa = 10.85VVEE460 pKa = 4.43TYY462 pKa = 11.29KK463 pKa = 10.96DD464 pKa = 3.3ITGKK468 pKa = 8.02TQTNIANKK476 pKa = 9.8KK477 pKa = 9.5CYY479 pKa = 9.61PDD481 pKa = 5.04FDD483 pKa = 5.89DD484 pKa = 5.96DD485 pKa = 5.13CADD488 pKa = 3.77HH489 pKa = 7.39LDD491 pKa = 4.0TEE493 pKa = 4.51VLRR496 pKa = 11.84ILACAYY502 pKa = 10.27SCEE505 pKa = 3.92ALKK508 pKa = 10.2MYY510 pKa = 9.94IYY512 pKa = 10.54EE513 pKa = 4.46PLPSVHH519 pKa = 6.58EE520 pKa = 4.49SYY522 pKa = 10.68KK523 pKa = 10.81GEE525 pKa = 4.38AYY527 pKa = 10.6GKK529 pKa = 8.49MKK531 pKa = 10.47PIDD534 pKa = 4.32LYY536 pKa = 11.42SLQKK540 pKa = 10.69SRR542 pKa = 11.84TGVIDD547 pKa = 3.09NRR549 pKa = 11.84TILSRR554 pKa = 11.84QEE556 pKa = 4.0PSLSTWEE563 pKa = 4.08KK564 pKa = 9.09TNSVMSKK571 pKa = 10.62AIASLDD577 pKa = 3.61KK578 pKa = 11.07LDD580 pKa = 3.87VSSISDD586 pKa = 3.7SNTLSRR592 pKa = 11.84TTLMYY597 pKa = 10.41SSKK600 pKa = 10.69VDD602 pKa = 3.19GKK604 pKa = 9.28YY605 pKa = 10.28RR606 pKa = 11.84YY607 pKa = 9.32MIEE610 pKa = 3.75QDD612 pKa = 4.5LISDD616 pKa = 4.15TGTVFEE622 pKa = 4.85MVDD625 pKa = 3.24GTMSHH630 pKa = 6.04SKK632 pKa = 10.93KK633 pKa = 10.94LMITHH638 pKa = 6.74KK639 pKa = 10.51NVGVTYY645 pKa = 9.4TIEE648 pKa = 4.01YY649 pKa = 9.92LYY651 pKa = 8.39TTADD655 pKa = 2.85IVTTKK660 pKa = 10.25TSIYY664 pKa = 10.27SSCTGNCEE672 pKa = 4.16KK673 pKa = 10.48CWEE676 pKa = 4.39GLGSTDD682 pKa = 2.96NPSYY686 pKa = 11.09HH687 pKa = 6.68KK688 pKa = 10.62FCLEE692 pKa = 3.85PTSQWGCEE700 pKa = 3.94DD701 pKa = 3.9FMCIAMNEE709 pKa = 4.14GSTCGHH715 pKa = 7.1CDD717 pKa = 3.06NVYY720 pKa = 11.44DD721 pKa = 4.29MSNSLDD727 pKa = 3.47VYY729 pKa = 9.88RR730 pKa = 11.84VKK732 pKa = 10.43EE733 pKa = 3.88VHH735 pKa = 6.32VSSTACVNAGKK746 pKa = 10.6GYY748 pKa = 10.65NCFNHH753 pKa = 7.67DD754 pKa = 4.28DD755 pKa = 3.82RR756 pKa = 11.84KK757 pKa = 10.95AYY759 pKa = 9.75TNDD762 pKa = 3.14NYY764 pKa = 10.6QVSITSTLQNDD775 pKa = 4.66YY776 pKa = 11.0ISVGHH781 pKa = 6.74KK782 pKa = 10.06LALAKK787 pKa = 10.71DD788 pKa = 4.17GSAYY792 pKa = 9.42TGNIADD798 pKa = 4.91LMSADD803 pKa = 3.69MTFGHH808 pKa = 6.59PQLNKK813 pKa = 10.11QGEE816 pKa = 4.28LLFAEE821 pKa = 4.93EE822 pKa = 4.63YY823 pKa = 9.98IGKK826 pKa = 8.58EE827 pKa = 3.75QLSWDD832 pKa = 4.18CEE834 pKa = 4.31FIGPKK839 pKa = 9.53KK840 pKa = 10.89VYY842 pKa = 9.54IKK844 pKa = 10.75KK845 pKa = 10.26CGFYY849 pKa = 10.39TFDD852 pKa = 3.89HH853 pKa = 6.65FSGLEE858 pKa = 3.76KK859 pKa = 10.72LNEE862 pKa = 4.01GSFVITGLNKK872 pKa = 8.67ITMLKK877 pKa = 10.25DD878 pKa = 3.25YY879 pKa = 10.91SLGKK883 pKa = 10.18IKK885 pKa = 9.72TVLDD889 pKa = 3.74LPIEE893 pKa = 4.21LFVEE897 pKa = 4.4TTEE900 pKa = 4.08APKK903 pKa = 11.11VNIIDD908 pKa = 4.29HH909 pKa = 6.24FCSGCLEE916 pKa = 4.28CFKK919 pKa = 11.28GLDD922 pKa = 3.66CKK924 pKa = 11.31YY925 pKa = 10.44KK926 pKa = 10.49INSNKK931 pKa = 9.57HH932 pKa = 3.82FASRR936 pKa = 11.84ISSDD940 pKa = 3.46FCVSKK945 pKa = 10.24HH946 pKa = 5.31QYY948 pKa = 10.65LIVEE952 pKa = 4.7RR953 pKa = 11.84GSNEE957 pKa = 2.98ISMNLHH963 pKa = 6.33CGVLPEE969 pKa = 3.99NAVLKK974 pKa = 10.01MIVEE978 pKa = 4.44DD979 pKa = 4.2NEE981 pKa = 4.52NISLDD986 pKa = 3.37IPLTKK991 pKa = 9.67IEE993 pKa = 4.98IINDD997 pKa = 3.52KK998 pKa = 10.83NVEE1001 pKa = 4.13TLKK1004 pKa = 10.73DD1005 pKa = 3.33HH1006 pKa = 7.68RR1007 pKa = 11.84IQKK1010 pKa = 9.67SFQHH1014 pKa = 6.9KK1015 pKa = 10.11EE1016 pKa = 3.67DD1017 pKa = 3.85TDD1019 pKa = 3.76FHH1021 pKa = 6.46TFLEE1025 pKa = 4.86TVTYY1029 pKa = 9.07PFNWVANFTNSALDD1043 pKa = 3.9LLKK1046 pKa = 10.81LALLFGFIILGLYY1059 pKa = 9.45VLSKK1063 pKa = 10.6CVNLGKK1069 pKa = 9.94EE1070 pKa = 4.44AYY1072 pKa = 9.26SHH1074 pKa = 5.29ATIYY1078 pKa = 10.27IKK1080 pKa = 10.53KK1081 pKa = 9.83KK1082 pKa = 10.2KK1083 pKa = 9.85HH1084 pKa = 6.8DD1085 pKa = 4.91DD1086 pKa = 3.56NDD1088 pKa = 4.23SDD1090 pKa = 4.21VDD1092 pKa = 3.76DD1093 pKa = 4.63SEE1095 pKa = 4.92MYY1097 pKa = 10.71GEE1099 pKa = 4.33KK1100 pKa = 10.71NLVRR1104 pKa = 11.84RR1105 pKa = 11.84LKK1107 pKa = 11.11YY1108 pKa = 9.83IDD1110 pKa = 3.53LL1111 pKa = 4.45

Molecular weight:
126.28 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D4D8M7|A0A0D4D8M7_9VIRU Envelopment polyprotein OS=Groundnut chlorotic fan-spot virus OX=1629551 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.21MISRR6 pKa = 11.84IGSSINSTVTNGLQNLGRR24 pKa = 11.84TNEE27 pKa = 4.1DD28 pKa = 3.06TDD30 pKa = 4.11VLGSDD35 pKa = 3.37NRR37 pKa = 11.84TLMRR41 pKa = 11.84MPTKK45 pKa = 10.26RR46 pKa = 11.84DD47 pKa = 3.14AEE49 pKa = 4.34KK50 pKa = 10.85ARR52 pKa = 11.84EE53 pKa = 3.81TRR55 pKa = 11.84LQGFKK60 pKa = 10.09EE61 pKa = 4.06RR62 pKa = 11.84NVIEE66 pKa = 4.58SGPSEE71 pKa = 4.0LGEE74 pKa = 3.88YY75 pKa = 10.32DD76 pKa = 3.9GNPVITSDD84 pKa = 4.21LSILEE89 pKa = 3.99RR90 pKa = 11.84LEE92 pKa = 4.65VNTSNHH98 pKa = 4.74ISNWKK103 pKa = 8.3TDD105 pKa = 3.17VFLGNGQEE113 pKa = 4.14MVKK116 pKa = 10.46KK117 pKa = 10.21DD118 pKa = 3.31INLIPTWDD126 pKa = 3.54SKK128 pKa = 11.32KK129 pKa = 10.88EE130 pKa = 3.71YY131 pKa = 8.67MQISRR136 pKa = 11.84VIIWIVPVAPGTKK149 pKa = 10.0GKK151 pKa = 10.25IKK153 pKa = 10.51AALVDD158 pKa = 3.9RR159 pKa = 11.84NKK161 pKa = 10.95AEE163 pKa = 3.9SEE165 pKa = 3.89QIIFQKK171 pKa = 10.63EE172 pKa = 4.09GVLTDD177 pKa = 3.7PLCFIFYY184 pKa = 7.23MHH186 pKa = 7.19WSYY189 pKa = 11.47LKK191 pKa = 10.66SVNNKK196 pKa = 9.12ALCPQLKK203 pKa = 9.9FISNEE208 pKa = 3.98VYY210 pKa = 10.42KK211 pKa = 11.01KK212 pKa = 10.27NVPFAVANFAWRR224 pKa = 11.84KK225 pKa = 7.36VFCNSPIAMTEE236 pKa = 3.92VQPDD240 pKa = 4.58LIVLNRR246 pKa = 11.84GITIRR251 pKa = 11.84NKK253 pKa = 10.69ALLEE257 pKa = 3.97AVKK260 pKa = 10.77CIVPNGNNGKK270 pKa = 7.83TIKK273 pKa = 10.38KK274 pKa = 9.99QIEE277 pKa = 4.37SLSKK281 pKa = 10.57SLEE284 pKa = 3.91AAALEE289 pKa = 4.71DD290 pKa = 4.07EE291 pKa = 4.78SEE293 pKa = 4.36STGAEE298 pKa = 4.02MKK300 pKa = 10.59EE301 pKa = 3.94LTFDD305 pKa = 3.89LL306 pKa = 4.9

Molecular weight:
34.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

4287

306

2870

1429.0

164.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.732 ± 0.393

2.379 ± 0.552

6.181 ± 0.256

6.461 ± 0.503

4.712 ± 0.34

4.129 ± 0.65

2.029 ± 0.31

7.488 ± 0.502

8.771 ± 0.264

9.447 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.732 ± 0.367

6.765 ± 0.374

2.892 ± 0.269

2.426 ± 0.108

3.569 ± 0.519

9.097 ± 0.312

5.482 ± 0.485

5.365 ± 0.333

0.77 ± 0.204

4.572 ± 0.598

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski