Proteome-pI 2.0 - Proteome Isoelectric Point Database

Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus; Thermus thermophilus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q5SH74|Q5SH74_THET8 ABC transporter permease protein OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=TTHA1856 PE=4 SV=1

MM1 pKa = 7.52GNYY4 pKa = 10.26LEE6 pKa = 5.65DD7 pKa = 4.18CATVDD12 pKa = 3.41VQARR16 pKa = 11.84PTAYY20 pKa = 10.27ALAISSLGEE29 pKa = 4.04FNSLTGGTSTDD40 pKa = 3.6PVAEE44 pKa = 4.46GNDD47 pKa = 3.23YY48 pKa = 11.05YY49 pKa = 11.68YY50 pKa = 10.81RR51 pKa = 11.84FEE53 pKa = 3.74IRR55 pKa = 11.84AWEE58 pKa = 4.78GSSGPQTNVTLNVTRR73 pKa = 11.84TLGNSTFAGSGTKK86 pKa = 10.44GVDD89 pKa = 3.89FEE91 pKa = 5.46VEE93 pKa = 4.25LDD95 pKa = 3.4PDD97 pKa = 4.38GPFGPASYY105 pKa = 11.05APVLSADD112 pKa = 3.81VQVLAWGPTGVQLRR126 pKa = 11.84YY127 pKa = 9.9LPSLAPGATLRR138 pKa = 11.84FSLRR142 pKa = 11.84ANAVNGTNTTVQADD156 pKa = 3.87ATSTEE161 pKa = 3.92APGPYY166 pKa = 9.06TVFEE170 pKa = 4.39TTTIIPP176 pKa = 3.81

Molecular weight: 18.47 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>sp|P80377|RS13_THET8 30S ribosomal protein S13 OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=rpsM PE=1 SV=3

MM1 pKa = 7.38KK2 pKa = 9.45RR3 pKa = 11.84TWQPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84AKK14 pKa = 8.56THH16 pKa = 5.27GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPGGRR28 pKa = 11.84KK29 pKa = 7.1VLKK32 pKa = 10.16RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.88GRR39 pKa = 11.84WRR41 pKa = 11.84LTPAVRR47 pKa = 11.84KK48 pKa = 9.58RR49 pKa = 3.3

Molecular weight: 6.11 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2227	0	2227	663962	11	2672	298.1	32.81

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.576 ± 0.064	0.393 ± 0.014	3.595 ± 0.029	8.617 ± 0.069	3.773 ± 0.036	9.326 ± 0.062	1.86 ± 0.023	2.595 ± 0.048	3.558 ± 0.04	14.526 ± 0.093

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.535 ± 0.018	1.557 ± 0.025	6.58 ± 0.049	2.445 ± 0.03	8.564 ± 0.054	3.44 ± 0.029	3.752 ± 0.036	8.052 ± 0.049	1.381 ± 0.022	2.875 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski