Fibularhizoctonia sp. CBS 109695
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31941 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166MQZ6|A0A166MQZ6_9AGAM TFIIIC_sub6 domain-containing protein OS=Fibularhizoctonia sp. CBS 109695 OX=436010 GN=FIBSPDRAFT_735970 PE=4 SV=1
MM1 pKa = 7.56 SSKK4 pKa = 8.94 QTSSGSGYY12 pKa = 10.9 AKK14 pKa = 10.71 NSVTGSGSNDD24 pKa = 3.45 QGNHH28 pKa = 4.26 YY29 pKa = 7.64 CTRR32 pKa = 11.84 EE33 pKa = 3.84 DD34 pKa = 3.37 SAGNSGYY41 pKa = 10.53 HH42 pKa = 5.64 YY43 pKa = 10.5 SNKK46 pKa = 10.3 DD47 pKa = 2.8 GSYY50 pKa = 10.64 YY51 pKa = 9.67 YY52 pKa = 10.51 QNPDD56 pKa = 2.48 GSKK59 pKa = 10.19 YY60 pKa = 10.54 YY61 pKa = 10.23 EE62 pKa = 4.03 NKK64 pKa = 10.41 AGNSKK69 pKa = 8.64 YY70 pKa = 9.28 TNPRR74 pKa = 11.84 GDD76 pKa = 3.7 TYY78 pKa = 11.45 VKK80 pKa = 10.48 SSSATVANDD89 pKa = 3.42 HH90 pKa = 7.03 GDD92 pKa = 3.42 IQDD95 pKa = 4.45 DD96 pKa = 3.95 RR97 pKa = 11.84 NGDD100 pKa = 3.55 HH101 pKa = 7.19 SSAAAEE107 pKa = 4.54 DD108 pKa = 4.72 GSDD111 pKa = 3.51 GGHH114 pKa = 6.89 ASDD117 pKa = 5.28 AGHH120 pKa = 5.96 SHH122 pKa = 7.49 DD123 pKa = 4.95 GSSDD127 pKa = 3.74 SEE129 pKa = 4.61 GSGSRR134 pKa = 11.84 PDD136 pKa = 4.19 AGQEE140 pKa = 3.99 DD141 pKa = 4.8 AVDD144 pKa = 4.0 DD145 pKa = 4.32 TDD147 pKa = 6.22 DD148 pKa = 3.97 EE149 pKa = 6.04 SDD151 pKa = 4.23 DD152 pKa = 4.12 EE153 pKa = 5.27 GSASLPNSDD162 pKa = 4.8 EE163 pKa = 4.22 EE164 pKa = 5.02 GSLDD168 pKa = 4.32 SPGGSDD174 pKa = 3.65 DD175 pKa = 4.15 SEE177 pKa = 4.72 SAGEE181 pKa = 3.94 SDD183 pKa = 4.51 EE184 pKa = 4.61 YY185 pKa = 11.63 SDD187 pKa = 5.74 DD188 pKa = 4.78 FEE190 pKa = 7.19 DD191 pKa = 4.08 EE192 pKa = 4.13
Molecular weight: 19.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.846
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.228
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.973
Patrickios 1.252
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A167XPE2|A0A167XPE2_9AGAM CHAT domain-containing protein (Fragment) OS=Fibularhizoctonia sp. CBS 109695 OX=436010 GN=FIBSPDRAFT_762943 PE=4 SV=1
MM1 pKa = 7.12 GRR3 pKa = 11.84 WAGLYY8 pKa = 9.25 RR9 pKa = 11.84 ARR11 pKa = 11.84 IALRR15 pKa = 11.84 PGRR18 pKa = 11.84 SSQLVRR24 pKa = 11.84 RR25 pKa = 11.84 PLSAASRR32 pKa = 11.84 SRR34 pKa = 11.84 FRR36 pKa = 11.84 PSLVAAPAAARR47 pKa = 11.84 RR48 pKa = 11.84 SSPVARR54 pKa = 11.84 RR55 pKa = 11.84 PSPVARR61 pKa = 11.84 RR62 pKa = 11.84 SSPVARR68 pKa = 11.84 RR69 pKa = 11.84 PSPVARR75 pKa = 11.84 RR76 pKa = 11.84 LSVAARR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 AGCWSLRR91 pKa = 11.84 RR92 pKa = 11.84 PLL94 pKa = 3.77
Molecular weight: 10.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.213
IPC_protein 12.705
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.866
Wikipedia 13.349
Rodwell 12.369
Grimsley 12.91
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.866
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.091
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31934
7
31941
10750530
49
5067
336.6
37.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.445 ± 0.013
1.522 ± 0.008
5.362 ± 0.011
5.467 ± 0.013
3.623 ± 0.008
6.753 ± 0.014
2.771 ± 0.007
4.959 ± 0.011
4.164 ± 0.013
9.298 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.199 ± 0.005
3.29 ± 0.008
6.298 ± 0.015
3.69 ± 0.009
6.232 ± 0.012
8.352 ± 0.018
6.089 ± 0.012
6.301 ± 0.011
1.554 ± 0.006
2.628 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here