Yellow-breasted capuchin simian foamy virus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3Y5C1|A0A0G3Y5C1_9RETR Env leader protein OS=Yellow-breasted capuchin simian foamy virus OX=2170206 GN=env PE=4 SV=1
MM1 pKa = 7.83 AAQQEE6 pKa = 4.41 EE7 pKa = 5.0 TEE9 pKa = 4.33 LKK11 pKa = 10.47 EE12 pKa = 4.1 FLAEE16 pKa = 4.85 FPFNDD21 pKa = 4.31 PDD23 pKa = 4.47 DD24 pKa = 5.05 DD25 pKa = 3.86 LWVPVNIPPAPFQPYY40 pKa = 10.4 ADD42 pKa = 3.64 PHH44 pKa = 7.77 DD45 pKa = 4.41 EE46 pKa = 4.38 EE47 pKa = 4.65 EE48 pKa = 5.29 GEE50 pKa = 4.17 YY51 pKa = 10.77 LLPKK55 pKa = 9.19 EE56 pKa = 4.33 QVFTTPSEE64 pKa = 4.37 AEE66 pKa = 3.71 TSEE69 pKa = 5.12 RR70 pKa = 11.84 DD71 pKa = 3.56 DD72 pKa = 4.56 PLPGTSTTINEE83 pKa = 4.36 PTGEE87 pKa = 4.01 EE88 pKa = 4.35 PKK90 pKa = 10.9 FKK92 pKa = 9.72 WLQVARR98 pKa = 11.84 RR99 pKa = 11.84 EE100 pKa = 3.95 TDD102 pKa = 2.76 YY103 pKa = 11.74 DD104 pKa = 3.84 RR105 pKa = 11.84 FDD107 pKa = 3.83 LGRR110 pKa = 11.84 FFPDD114 pKa = 2.73 RR115 pKa = 11.84 LQRR118 pKa = 11.84 EE119 pKa = 5.03 FILQRR124 pKa = 11.84 AILTAAGYY132 pKa = 10.05 QPTLVEE138 pKa = 4.65 HH139 pKa = 6.28 CAKK142 pKa = 9.7 MGWYY146 pKa = 10.23 ACLQPHH152 pKa = 6.79 HH153 pKa = 6.7 GALGEE158 pKa = 4.29 TTEE161 pKa = 4.86 VYY163 pKa = 10.11 YY164 pKa = 10.93 KK165 pKa = 10.51 CLKK168 pKa = 10.29 CGSEE172 pKa = 3.79 QWDD175 pKa = 3.66 PLLYY179 pKa = 10.11 IWDD182 pKa = 3.48 KK183 pKa = 11.11 HH184 pKa = 4.11 MLMFKK189 pKa = 10.21 RR190 pKa = 11.84 AWTRR194 pKa = 11.84 TPHH197 pKa = 5.69 TSSPLSNLRR206 pKa = 11.84 RR207 pKa = 11.84 HH208 pKa = 6.6 DD209 pKa = 4.91 AICPGLNPLLPHH221 pKa = 6.93 SSTEE225 pKa = 3.68 PLLRR229 pKa = 11.84 KK230 pKa = 9.19 PRR232 pKa = 11.84 RR233 pKa = 11.84 DD234 pKa = 3.22 PGDD237 pKa = 2.89 RR238 pKa = 11.84 WRR240 pKa = 11.84 EE241 pKa = 3.74 RR242 pKa = 11.84 KK243 pKa = 9.56 RR244 pKa = 11.84 KK245 pKa = 8.95 ISRR248 pKa = 11.84 GGPTAVLPADD258 pKa = 3.64 TFTYY262 pKa = 10.26 ASEE265 pKa = 4.18 WPQPEE270 pKa = 4.12 RR271 pKa = 11.84 MHH273 pKa = 7.21 SGGLGTVPILVQTMQSKK290 pKa = 9.92 CARR293 pKa = 11.84 FEE295 pKa = 3.98 MEE297 pKa = 3.83 TLQNRR302 pKa = 11.84 GIVV305 pKa = 3.3
Molecular weight: 35.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.308
IPC2_protein 5.321
IPC_protein 5.296
Toseland 5.423
ProMoST 5.512
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.283
Rodwell 5.321
Grimsley 5.423
Solomon 5.372
Lehninger 5.347
Nozaki 5.55
DTASelect 5.69
Thurlkill 5.499
EMBOSS 5.448
Sillero 5.639
Patrickios 4.139
IPC_peptide 5.385
IPC2_peptide 5.639
IPC2.peptide.svr19 5.598
Protein with the highest isoelectric point:
>tr|A0A0G3Y3E1|A0A0G3Y3E1_9RETR Tas protein OS=Yellow-breasted capuchin simian foamy virus OX=2170206 GN=tas PE=4 SV=1
MM1 pKa = 7.28 TAPPLLQLPVEE12 pKa = 4.51 VKK14 pKa = 9.23 KK15 pKa = 9.51 TEE17 pKa = 4.54 LNGFWDD23 pKa = 3.81 TGAQITCIPEE33 pKa = 3.99 AFLKK37 pKa = 10.8 EE38 pKa = 4.14 EE39 pKa = 4.1 IPIGEE44 pKa = 4.11 AQIKK48 pKa = 7.37 TLHH51 pKa = 5.44 GTKK54 pKa = 9.92 LQPVYY59 pKa = 10.51 YY60 pKa = 10.18 LKK62 pKa = 10.9 FKK64 pKa = 10.56 ILGRR68 pKa = 11.84 KK69 pKa = 8.75 VEE71 pKa = 4.29 AEE73 pKa = 4.16 VTTSPFDD80 pKa = 3.54 YY81 pKa = 11.04 VIISPSDD88 pKa = 3.41 IPWYY92 pKa = 10.27 KK93 pKa = 10.0 PQPLEE98 pKa = 4.12 LTVKK102 pKa = 10.81 LPVQDD107 pKa = 4.66 FKK109 pKa = 11.8 KK110 pKa = 10.75 EE111 pKa = 4.23 LINKK115 pKa = 9.37 ANINNEE121 pKa = 4.14 EE122 pKa = 4.11 KK123 pKa = 10.66 KK124 pKa = 10.53 QLAKK128 pKa = 11.01 LLDD131 pKa = 4.07 KK132 pKa = 11.4 YY133 pKa = 11.2 DD134 pKa = 4.05 ILWQQWEE141 pKa = 4.33 NQVGHH146 pKa = 6.68 RR147 pKa = 11.84 KK148 pKa = 9.22 IPPHH152 pKa = 6.48 NIATGTVAPRR162 pKa = 11.84 PQRR165 pKa = 11.84 QYY167 pKa = 11.27 HH168 pKa = 6.52 INTKK172 pKa = 10.09 AKK174 pKa = 10.19 PSIQQVIDD182 pKa = 3.96 DD183 pKa = 4.51 LLKK186 pKa = 10.74 QGVLVKK192 pKa = 9.25 QTSVMNTPVYY202 pKa = 9.64 PVPKK206 pKa = 9.66 PDD208 pKa = 3.59 GKK210 pKa = 9.91 WRR212 pKa = 11.84 MVLDD216 pKa = 3.44 YY217 pKa = 10.78 RR218 pKa = 11.84 AVNKK222 pKa = 7.69 TIPLIGAQNQHH233 pKa = 5.82 SLGILTNLVRR243 pKa = 11.84 QKK245 pKa = 10.91 YY246 pKa = 9.88 KK247 pKa = 10.25 STIDD251 pKa = 3.45 LSNGFWAHH259 pKa = 7.56 PITKK263 pKa = 9.88 DD264 pKa = 3.77 SQWITAFTWEE274 pKa = 4.61 GKK276 pKa = 6.41 QHH278 pKa = 4.39 VWTRR282 pKa = 11.84 LPQGFLNSPALFTADD297 pKa = 4.02 VVDD300 pKa = 4.66 LLKK303 pKa = 10.56 DD304 pKa = 3.19 IPGISVYY311 pKa = 10.76 VDD313 pKa = 4.41 DD314 pKa = 5.79 IYY316 pKa = 11.35 FSTEE320 pKa = 3.79 TVSEE324 pKa = 4.05 HH325 pKa = 6.73 LKK327 pKa = 10.38 ILEE330 pKa = 4.2 KK331 pKa = 10.65 VFKK334 pKa = 10.28 ILLEE338 pKa = 4.2 AGYY341 pKa = 9.96 IVSLKK346 pKa = 10.46 KK347 pKa = 10.39 SALLRR352 pKa = 11.84 HH353 pKa = 5.81 EE354 pKa = 4.26 VTFLGFSITQTGRR367 pKa = 11.84 GLTSEE372 pKa = 4.86 FKK374 pKa = 11.01 DD375 pKa = 3.35 KK376 pKa = 10.64 IQNITPPKK384 pKa = 8.33 TLKK387 pKa = 10.15 EE388 pKa = 4.02 LQSILGLFNFARR400 pKa = 11.84 NFVPNFSEE408 pKa = 4.44 IIKK411 pKa = 9.68 PLYY414 pKa = 10.46 SLISTAEE421 pKa = 3.92 GNNIKK426 pKa = 8.34 WTSEE430 pKa = 3.75 HH431 pKa = 5.72 TRR433 pKa = 11.84 HH434 pKa = 6.45 LEE436 pKa = 4.31 EE437 pKa = 4.54 IVSALNHH444 pKa = 6.5 AGNLEE449 pKa = 3.92 QRR451 pKa = 11.84 DD452 pKa = 4.16 DD453 pKa = 3.97 EE454 pKa = 4.72 SPLVVKK460 pKa = 10.42 LNASPKK466 pKa = 8.09 TGYY469 pKa = 9.03 IRR471 pKa = 11.84 YY472 pKa = 8.85 YY473 pKa = 10.79 NKK475 pKa = 10.51 GGQKK479 pKa = 9.37 PIAYY483 pKa = 9.15 ASHH486 pKa = 6.72 VFTNTEE492 pKa = 4.05 SKK494 pKa = 9.07 FTPLEE499 pKa = 4.07 KK500 pKa = 10.8 LLVTMHH506 pKa = 7.05 KK507 pKa = 10.73 ALIKK511 pKa = 10.8 AIDD514 pKa = 3.85 LALGQPIEE522 pKa = 4.45 VYY524 pKa = 10.75 SPIVSMQKK532 pKa = 9.77 LQKK535 pKa = 9.64 TPLPEE540 pKa = 4.18 RR541 pKa = 11.84 KK542 pKa = 9.46 ALSTRR547 pKa = 11.84 WITWLSYY554 pKa = 11.25 LEE556 pKa = 4.15 DD557 pKa = 3.56 PRR559 pKa = 11.84 IIFHH563 pKa = 6.39 YY564 pKa = 10.71 DD565 pKa = 2.58 KK566 pKa = 10.79 TLPDD570 pKa = 4.04 LKK572 pKa = 10.68 NVPEE576 pKa = 4.86 TITEE580 pKa = 4.13 KK581 pKa = 10.46 QPKK584 pKa = 8.45 ILPIIEE590 pKa = 4.23 YY591 pKa = 10.39 AAVFYY596 pKa = 10.65 TDD598 pKa = 3.37 GSAIRR603 pKa = 11.84 SPDD606 pKa = 3.12 KK607 pKa = 11.19 NKK609 pKa = 10.31 SHH611 pKa = 6.66 SSGMGIVQAIFKK623 pKa = 10.26 PEE625 pKa = 3.77 LTIEE629 pKa = 4.31 HH630 pKa = 6.35 QWTIPLGDD638 pKa = 3.58 HH639 pKa = 5.95 TAQYY643 pKa = 11.49 AEE645 pKa = 3.83 ISAVEE650 pKa = 4.21 FACKK654 pKa = 9.69 KK655 pKa = 10.2 ANNISGPVLIVTDD668 pKa = 3.44 SDD670 pKa = 4.12 YY671 pKa = 11.43 VARR674 pKa = 11.84 SVNEE678 pKa = 3.8 EE679 pKa = 3.95 LPFWRR684 pKa = 11.84 SNGFVNNKK692 pKa = 9.32 KK693 pKa = 10.41 KK694 pKa = 9.96 PLKK697 pKa = 10.46 HH698 pKa = 5.21 ISKK701 pKa = 9.25 WKK703 pKa = 10.21 NISDD707 pKa = 3.87 SLLLKK712 pKa = 10.21 RR713 pKa = 11.84 DD714 pKa = 3.11 ITIVHH719 pKa = 6.41 EE720 pKa = 4.73 PGHH723 pKa = 5.75 QPSHH727 pKa = 5.76 TSIHH731 pKa = 4.92 TQGNNLADD739 pKa = 3.7 KK740 pKa = 10.66 LATQGSYY747 pKa = 10.61 NVNSIVKK754 pKa = 9.9 NPSLDD759 pKa = 3.61 AEE761 pKa = 4.47 LEE763 pKa = 4.03 QLINGHH769 pKa = 5.51 SMKK772 pKa = 10.45 GYY774 pKa = 7.63 PSKK777 pKa = 11.0 YY778 pKa = 10.15 KK779 pKa = 10.85 YY780 pKa = 9.77 ILKK783 pKa = 9.88 EE784 pKa = 3.71 GQVFVLRR791 pKa = 11.84 PEE793 pKa = 4.33 GEE795 pKa = 4.05 KK796 pKa = 10.11 IIPPKK801 pKa = 10.06 SDD803 pKa = 2.8 RR804 pKa = 11.84 PALVKK809 pKa = 10.18 VAHH812 pKa = 6.42 EE813 pKa = 4.39 FSHH816 pKa = 6.99 AGRR819 pKa = 11.84 EE820 pKa = 4.33 ATVLRR825 pKa = 11.84 LQDD828 pKa = 3.73 KK829 pKa = 9.13 YY830 pKa = 9.53 WWPNMRR836 pKa = 11.84 KK837 pKa = 9.73 DD838 pKa = 4.01 VISHH842 pKa = 5.81 IRR844 pKa = 11.84 MCKK847 pKa = 9.43 PCLTTDD853 pKa = 3.45 SSNLTPIPPKK863 pKa = 9.62 PQQRR867 pKa = 11.84 PKK869 pKa = 10.99 KK870 pKa = 9.28 PFDD873 pKa = 3.57 KK874 pKa = 11.01 FFIDD878 pKa = 4.29 YY879 pKa = 10.24 IGPLPPSHH887 pKa = 6.3 GFAYY891 pKa = 10.62 VLVVVDD897 pKa = 4.38 AATGFTWLYY906 pKa = 5.84 PTKK909 pKa = 10.62 APSTNATITSLNILLGTAVPRR930 pKa = 11.84 VLHH933 pKa = 6.51 SDD935 pKa = 2.92 QGSAFTSSAFADD947 pKa = 3.46 WAKK950 pKa = 10.97 EE951 pKa = 3.67 KK952 pKa = 10.79 GIQLEE957 pKa = 4.54 FSTPYY962 pKa = 10.11 HH963 pKa = 5.86 PQSSGMVEE971 pKa = 3.9 RR972 pKa = 11.84 KK973 pKa = 9.31 NRR975 pKa = 11.84 EE976 pKa = 3.56 IKK978 pKa = 10.6 RR979 pKa = 11.84 LITKK983 pKa = 9.73 LLVGRR988 pKa = 11.84 PTKK991 pKa = 9.9 WYY993 pKa = 9.08 PLIPTIQLALNNTYY1007 pKa = 10.43 SVHH1010 pKa = 5.57 YY1011 pKa = 9.93 KK1012 pKa = 8.78 KK1013 pKa = 9.09 TPHH1016 pKa = 5.97 QLLFGVDD1023 pKa = 3.65 GNVPFANQDD1032 pKa = 3.55 TLDD1035 pKa = 3.66 LTRR1038 pKa = 11.84 EE1039 pKa = 4.19 EE1040 pKa = 4.36 EE1041 pKa = 4.11 LSLLSEE1047 pKa = 4.5 VRR1049 pKa = 11.84 TSLLPPSTPPASRR1062 pKa = 11.84 RR1063 pKa = 11.84 SWLPSVGLLVQEE1075 pKa = 4.63 RR1076 pKa = 11.84 VARR1079 pKa = 11.84 PSQLRR1084 pKa = 11.84 PKK1086 pKa = 8.41 WRR1088 pKa = 11.84 KK1089 pKa = 5.65 PTPILEE1095 pKa = 4.4 VVNDD1099 pKa = 3.9 RR1100 pKa = 11.84 TVVILDD1106 pKa = 3.4 NQGQRR1111 pKa = 11.84 RR1112 pKa = 11.84 TVSIDD1117 pKa = 3.24 NLKK1120 pKa = 8.92 LTPHH1124 pKa = 6.49 QDD1126 pKa = 3.12 GTSNEE1131 pKa = 4.16 PNGVDD1136 pKa = 3.34 PLEE1139 pKa = 4.1 QEE1141 pKa = 4.51 KK1142 pKa = 10.3 EE1143 pKa = 4.02 HH1144 pKa = 7.19 DD1145 pKa = 3.55 NHH1147 pKa = 7.62 DD1148 pKa = 3.37 II1149 pKa = 4.09
Molecular weight: 130.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.703
IPC2_protein 8.653
IPC_protein 8.492
Toseland 9.355
ProMoST 9.077
Dawson 9.619
Bjellqvist 9.282
Wikipedia 9.765
Rodwell 9.97
Grimsley 9.677
Solomon 9.633
Lehninger 9.589
Nozaki 9.385
DTASelect 9.268
Thurlkill 9.458
EMBOSS 9.78
Sillero 9.531
Patrickios 5.029
IPC_peptide 9.633
IPC2_peptide 7.702
IPC2.peptide.svr19 7.752
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3429
305
1149
685.8
77.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.628 ± 0.529
1.633 ± 0.626
4.608 ± 0.168
5.862 ± 0.576
3.12 ± 0.182
5.745 ± 0.927
2.45 ± 0.324
6.183 ± 1.073
5.92 ± 1.295
9.536 ± 0.537
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.604 ± 0.427
5.133 ± 0.55
8.107 ± 1.17
5.016 ± 0.303
4.958 ± 0.748
6.328 ± 0.688
6.737 ± 0.325
5.862 ± 0.367
2.071 ± 0.24
3.5 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here