Gammarus sp. amphipod associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RLR1|A0A0K1RLR1_9CIRC Putative replication initiation protein OS=Gammarus sp. amphipod associated circular virus OX=1692250 PE=4 SV=1
MM1 pKa = 7.17TSCRR5 pKa = 11.84YY6 pKa = 9.94CFTVNNPTVQDD17 pKa = 3.74RR18 pKa = 11.84EE19 pKa = 4.2RR20 pKa = 11.84LDD22 pKa = 4.66LLADD26 pKa = 3.44SCNYY30 pKa = 9.87LVYY33 pKa = 10.65GNEE36 pKa = 4.17IGSSGTPHH44 pKa = 6.44LQGFVIFPKK53 pKa = 9.85TKK55 pKa = 9.72RR56 pKa = 11.84FNAAKK61 pKa = 9.99IAIGNTAHH69 pKa = 6.2VEE71 pKa = 4.16CARR74 pKa = 11.84GSSVQAATYY83 pKa = 9.48CKK85 pKa = 10.0KK86 pKa = 10.81DD87 pKa = 2.88GDD89 pKa = 3.68FRR91 pKa = 11.84EE92 pKa = 4.58FGEE95 pKa = 4.77LPSSQGKK102 pKa = 7.62RR103 pKa = 11.84TDD105 pKa = 2.66WDD107 pKa = 3.33IYY109 pKa = 10.58RR110 pKa = 11.84DD111 pKa = 3.42WVTDD115 pKa = 3.88LGRR118 pKa = 11.84VPSKK122 pKa = 11.02KK123 pKa = 10.0EE124 pKa = 3.49LVLAFPGFYY133 pKa = 10.5ARR135 pKa = 11.84YY136 pKa = 9.09RR137 pKa = 11.84KK138 pKa = 10.09ACFEE142 pKa = 4.07YY143 pKa = 11.04AEE145 pKa = 4.91ALTPPPILTQSEE157 pKa = 4.61PRR159 pKa = 11.84FGWQTRR165 pKa = 11.84VDD167 pKa = 4.22GIINGEE173 pKa = 4.01ANDD176 pKa = 3.92RR177 pKa = 11.84TIHH180 pKa = 6.02FVVDD184 pKa = 3.44PEE186 pKa = 4.41GNAGKK191 pKa = 8.11TWFCSYY197 pKa = 11.61ALTKK201 pKa = 10.18WPDD204 pKa = 3.0KK205 pKa = 9.06VQVMRR210 pKa = 11.84IGKK213 pKa = 9.5RR214 pKa = 11.84DD215 pKa = 3.69DD216 pKa = 3.16LAYY219 pKa = 10.39AISTEE224 pKa = 3.86KK225 pKa = 10.77SIFLMDD231 pKa = 3.52VPRR234 pKa = 11.84NQMTFLQYY242 pKa = 11.01SVLEE246 pKa = 4.04MLKK249 pKa = 10.78DD250 pKa = 3.7RR251 pKa = 11.84MIFSPKK257 pKa = 9.59YY258 pKa = 8.08EE259 pKa = 4.15SSFKK263 pKa = 9.92ILQYY267 pKa = 9.8VPHH270 pKa = 6.86VIVFSNEE277 pKa = 3.88QPDD280 pKa = 4.11TSALSADD287 pKa = 4.82RR288 pKa = 11.84INIINVV294 pKa = 3.01

Molecular weight: 33.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RLR1|A0A0K1RLR1_9CIRC Putative replication initiation protein OS=Gammarus sp. amphipod associated circular virus OX=1692250 PE=4 SV=1
MM1 pKa = 8.29PYY3 pKa = 9.65RR4 pKa = 11.84KK5 pKa = 9.45SYY7 pKa = 10.34RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TSRR13 pKa = 11.84KK14 pKa = 7.88RR15 pKa = 11.84FVRR18 pKa = 11.84RR19 pKa = 11.84PRR21 pKa = 11.84KK22 pKa = 9.47ARR24 pKa = 11.84AYY26 pKa = 8.12RR27 pKa = 11.84QKK29 pKa = 10.82RR30 pKa = 11.84GLDD33 pKa = 3.23KK34 pKa = 10.87TRR36 pKa = 11.84IGATPGSRR44 pKa = 11.84APCKK48 pKa = 10.33RR49 pKa = 11.84ITKK52 pKa = 9.88INQTTAQGQNDD63 pKa = 4.17RR64 pKa = 11.84EE65 pKa = 4.29LYY67 pKa = 10.37VYY69 pKa = 10.35PEE71 pKa = 4.04LTAIPKK77 pKa = 10.29GDD79 pKa = 4.49AINNRR84 pKa = 11.84QRR86 pKa = 11.84DD87 pKa = 3.43VCTIVGFRR95 pKa = 11.84INWEE99 pKa = 4.01MTVDD103 pKa = 3.66TRR105 pKa = 11.84NAMYY109 pKa = 9.84WNMAIVCPKK118 pKa = 10.22PKK120 pKa = 10.58ARR122 pKa = 11.84DD123 pKa = 3.28LAFQTGDD130 pKa = 3.31NVSTEE135 pKa = 3.74QFFRR139 pKa = 11.84GDD141 pKa = 3.24NQQRR145 pKa = 11.84GEE147 pKa = 4.31DD148 pKa = 3.81FSLSLTSQEE157 pKa = 3.89MHH159 pKa = 6.49YY160 pKa = 9.62KK161 pKa = 9.71TINSDD166 pKa = 3.77LYY168 pKa = 10.52NVLWRR173 pKa = 11.84KK174 pKa = 10.09KK175 pKa = 8.61MLINGSLPLSNEE187 pKa = 3.53EE188 pKa = 4.53QIAQNASSGKK198 pKa = 9.98NYY200 pKa = 10.47RR201 pKa = 11.84IGQKK205 pKa = 9.9YY206 pKa = 9.67IKK208 pKa = 10.17LNRR211 pKa = 11.84QMMFEE216 pKa = 5.05DD217 pKa = 3.59NQSNYY222 pKa = 10.0PIYY225 pKa = 10.74LLTWGARR232 pKa = 11.84FEE234 pKa = 4.7ADD236 pKa = 2.59SGAPISAGSPLAVRR250 pKa = 11.84MRR252 pKa = 11.84VLTFFRR258 pKa = 11.84EE259 pKa = 4.14PRR261 pKa = 11.84NN262 pKa = 3.51

Molecular weight: 30.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

556

262

294

278.0

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.835 ± 0.023

1.799 ± 0.425

5.576 ± 0.648

4.676 ± 0.311

4.856 ± 0.676

5.755 ± 0.02

0.899 ± 0.337

5.935 ± 0.137

5.935 ± 0.112

6.295 ± 0.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.48

5.755 ± 0.974

5.216 ± 0.165

4.676 ± 0.683

8.993 ± 1.848

6.475 ± 0.239

6.115 ± 0.005

5.396 ± 1.027

1.619 ± 0.06

4.496 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski