Klebsiella phage ST974-OXA48phi18.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J8V6|A0A482J8V6_9CAUD ClpX superfamily protein OS=Klebsiella phage ST974-OXA48phi18.2 OX=2555918 PE=4 SV=1
MM1 pKa = 7.05STMFALVLTVSMLTGGNQDD20 pKa = 3.27VLLGVYY26 pKa = 8.61DD27 pKa = 4.05TEE29 pKa = 4.46NDD31 pKa = 3.39CKK33 pKa = 10.75AAAEE37 pKa = 4.13EE38 pKa = 3.94QHH40 pKa = 6.7VKK42 pKa = 10.68AEE44 pKa = 4.33CYY46 pKa = 9.07PLKK49 pKa = 10.68GVLDD53 pKa = 3.7EE54 pKa = 5.04HH55 pKa = 6.8PAGFTVQMM63 pKa = 4.6

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J503|A0A482J503_9CAUD Transposase OS=Klebsiella phage ST974-OXA48phi18.2 OX=2555918 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84LINRR6 pKa = 11.84SKK8 pKa = 10.42QSPLGRR14 pKa = 11.84QACDD18 pKa = 2.61AALAKK23 pKa = 10.32HH24 pKa = 5.56VEE26 pKa = 4.22LYY28 pKa = 10.55GAYY31 pKa = 9.92GRR33 pKa = 11.84QKK35 pKa = 9.18TKK37 pKa = 9.25RR38 pKa = 11.84TYY40 pKa = 8.54TVVVQGSKK48 pKa = 8.64ITVEE52 pKa = 4.03VVNRR56 pKa = 11.84KK57 pKa = 8.92SSYY60 pKa = 9.49VATAMSCARR69 pKa = 11.84RR70 pKa = 11.84LHH72 pKa = 6.21HH73 pKa = 7.43LPGQCNN79 pKa = 3.0

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15529

37

1022

194.1

21.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.692 ± 0.375

1.211 ± 0.123

5.815 ± 0.253

5.937 ± 0.327

3.522 ± 0.21

7.245 ± 0.282

1.81 ± 0.164

5.577 ± 0.197

5.274 ± 0.354

9.17 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.146

3.696 ± 0.172

4.45 ± 0.251

4.36 ± 0.318

5.957 ± 0.275

6.221 ± 0.255

6.008 ± 0.415

6.852 ± 0.26

1.591 ± 0.126

3.11 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski