Streptococcus phage Javan630
Average proteome isoelectric point is 5.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BGL9|A0A4D6BGL9_9CAUD Virulence-related protein OS=Streptococcus phage Javan630 OX=2548289 GN=Javan630_0038 PE=4 SV=1
MM1 pKa = 7.7 SILEE5 pKa = 4.28 DD6 pKa = 3.6 LQVVLEE12 pKa = 4.23 TLDD15 pKa = 3.55 VPIEE19 pKa = 4.05 TGVFSDD25 pKa = 4.54 AAPSKK30 pKa = 10.82 YY31 pKa = 9.55 MVIVPMSDD39 pKa = 3.1 SFDD42 pKa = 3.48 LHH44 pKa = 7.72 SDD46 pKa = 3.43 NLPCIDD52 pKa = 3.61 VQEE55 pKa = 4.2 ARR57 pKa = 11.84 ISIYY61 pKa = 11.02 SKK63 pKa = 11.04 GSYY66 pKa = 8.2 TALKK70 pKa = 9.15 NQVVQLLLDD79 pKa = 3.5 SSFTITARR87 pKa = 11.84 SYY89 pKa = 10.9 IGFEE93 pKa = 4.5 DD94 pKa = 3.49 DD95 pKa = 2.56 TGYY98 pKa = 11.04 YY99 pKa = 9.56 HH100 pKa = 7.27 YY101 pKa = 11.1 NVDD104 pKa = 3.47 VAKK107 pKa = 10.29 HH108 pKa = 4.82 YY109 pKa = 10.36 EE110 pKa = 3.76 IGGNN114 pKa = 3.43
Molecular weight: 12.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.953
IPC2_protein 4.177
IPC_protein 4.113
Toseland 3.91
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.037
Rodwell 3.948
Grimsley 3.821
Solomon 4.088
Lehninger 4.05
Nozaki 4.215
DTASelect 4.457
Thurlkill 3.961
EMBOSS 4.05
Sillero 4.24
Patrickios 0.693
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.103
Protein with the highest isoelectric point:
>tr|A0A4D6BHF4|A0A4D6BHF4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan630 OX=2548289 GN=Javan630_0053 PE=4 SV=1
MM1 pKa = 7.87 AKK3 pKa = 10.22 RR4 pKa = 11.84 NTKK7 pKa = 7.8 QTSKK11 pKa = 10.51 RR12 pKa = 11.84 VASKK16 pKa = 10.74 ASKK19 pKa = 9.85 ILNDD23 pKa = 3.02 GRR25 pKa = 11.84 YY26 pKa = 8.36 SKK28 pKa = 10.57 KK29 pKa = 9.92 SKK31 pKa = 10.53 SVAGSALSQTRR42 pKa = 11.84 PSKK45 pKa = 10.8 KK46 pKa = 9.83 KK47 pKa = 10.19
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 10.467
IPC_protein 11.491
Toseland 11.784
ProMoST 12.062
Dawson 11.798
Bjellqvist 11.667
Wikipedia 12.149
Rodwell 12.106
Grimsley 11.828
Solomon 12.164
Lehninger 12.076
Nozaki 11.769
DTASelect 11.667
Thurlkill 11.769
EMBOSS 12.237
Sillero 11.769
Patrickios 11.842
IPC_peptide 12.164
IPC2_peptide 11.038
IPC2.peptide.svr19 8.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
14506
42
868
259.0
29.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.99 ± 0.371
1.103 ± 0.133
6.659 ± 0.26
7.804 ± 0.365
3.605 ± 0.151
6.604 ± 0.217
1.565 ± 0.127
7.19 ± 0.292
7.418 ± 0.458
8.514 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.161
4.901 ± 0.199
3.343 ± 0.153
3.068 ± 0.22
4.467 ± 0.259
6.646 ± 0.32
5.563 ± 0.332
6.266 ± 0.211
1.434 ± 0.154
4.081 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here