Aspergillus parasiticus
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13752 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6DKR6|A0A5N6DKR6_ASPPA Major facilitator superfamily domain-containing protein OS=Aspergillus parasiticus OX=5067 GN=BDV34DRAFT_235060 PE=4 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.51 EE4 pKa = 5.26 AGSIYY9 pKa = 10.71 DD10 pKa = 3.85 EE11 pKa = 4.95 IEE13 pKa = 4.34 IEE15 pKa = 5.79 DD16 pKa = 3.79 MTFDD20 pKa = 6.24 PITQLYY26 pKa = 9.73 HH27 pKa = 5.63 YY28 pKa = 7.55 PCPCGDD34 pKa = 3.67 RR35 pKa = 11.84 FEE37 pKa = 5.7 IMIDD41 pKa = 3.49 DD42 pKa = 4.19 LRR44 pKa = 11.84 DD45 pKa = 3.58 GEE47 pKa = 4.73 EE48 pKa = 3.84 IAVCPSCSLKK58 pKa = 10.28 IRR60 pKa = 11.84 VIFDD64 pKa = 3.02 VDD66 pKa = 4.13 DD67 pKa = 3.69 LHH69 pKa = 9.25 KK70 pKa = 10.81 DD71 pKa = 3.63 DD72 pKa = 4.29 QQQGPSAVAVQAA84 pKa = 4.09
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A5N6E2D3|A0A5N6E2D3_ASPPA Uncharacterized protein OS=Aspergillus parasiticus OX=5067 GN=BDV34DRAFT_219632 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.51 TRR48 pKa = 11.84 LGLL51 pKa = 4.09
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13752
0
13752
6381041
49
9294
464.0
51.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.189 ± 0.017
1.37 ± 0.008
5.57 ± 0.015
6.031 ± 0.019
3.868 ± 0.011
6.813 ± 0.017
2.462 ± 0.01
5.202 ± 0.014
4.555 ± 0.017
9.245 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.177 ± 0.007
3.724 ± 0.01
5.84 ± 0.022
3.992 ± 0.013
5.962 ± 0.015
8.235 ± 0.02
5.951 ± 0.012
6.28 ± 0.015
1.549 ± 0.007
2.984 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here