Amino acid dipepetide frequency for Cyanophage NATL2A-133

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.243AlaAla: 10.243 ± 1.324
0.96AlaCys: 0.96 ± 0.296
8.323AlaAsp: 8.323 ± 1.629
4.962AlaGlu: 4.962 ± 0.753
2.801AlaPhe: 2.801 ± 0.444
7.682AlaGly: 7.682 ± 0.996
1.2AlaHis: 1.2 ± 0.349
6.242AlaIle: 6.242 ± 0.753
6.882AlaLys: 6.882 ± 1.395
7.682AlaLeu: 7.682 ± 1.022
2.241AlaMet: 2.241 ± 0.479
5.442AlaAsn: 5.442 ± 0.886
3.121AlaPro: 3.121 ± 0.598
3.281AlaGln: 3.281 ± 0.475
4.481AlaArg: 4.481 ± 0.884
5.602AlaSer: 5.602 ± 0.845
6.642AlaThr: 6.642 ± 1.058
6.402AlaVal: 6.402 ± 1.282
0.8AlaTrp: 0.8 ± 0.212
2.641AlaTyr: 2.641 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.48CysAla: 0.48 ± 0.236
0.08CysCys: 0.08 ± 0.076
0.56CysAsp: 0.56 ± 0.211
0.56CysGlu: 0.56 ± 0.181
0.08CysPhe: 0.08 ± 0.085
0.48CysGly: 0.48 ± 0.176
0.08CysHis: 0.08 ± 0.082
0.48CysIle: 0.48 ± 0.2
0.56CysLys: 0.56 ± 0.211
0.8CysLeu: 0.8 ± 0.322
0.08CysMet: 0.08 ± 0.071
0.24CysAsn: 0.24 ± 0.131
0.48CysPro: 0.48 ± 0.215
0.16CysGln: 0.16 ± 0.103
0.32CysArg: 0.32 ± 0.168
0.56CysSer: 0.56 ± 0.169
0.72CysThr: 0.72 ± 0.302
0.72CysVal: 0.72 ± 0.223
0.0CysTrp: 0.0 ± 0.0
0.32CysTyr: 0.32 ± 0.195
0.0CysXaa: 0.0 ± 0.0
Asp
7.042AspAla: 7.042 ± 1.185
0.16AspCys: 0.16 ± 0.11
4.722AspAsp: 4.722 ± 0.668
4.081AspGlu: 4.081 ± 0.866
2.241AspPhe: 2.241 ± 0.47
5.522AspGly: 5.522 ± 0.779
0.72AspHis: 0.72 ± 0.215
4.962AspIle: 4.962 ± 0.749
3.201AspLys: 3.201 ± 0.582
5.362AspLeu: 5.362 ± 0.595
1.44AspMet: 1.44 ± 0.369
4.161AspAsn: 4.161 ± 0.661
2.561AspPro: 2.561 ± 0.603
2.081AspGln: 2.081 ± 0.334
1.841AspArg: 1.841 ± 0.468
4.962AspSer: 4.962 ± 0.679
4.241AspThr: 4.241 ± 0.863
2.961AspVal: 2.961 ± 0.369
1.04AspTrp: 1.04 ± 0.273
2.481AspTyr: 2.481 ± 0.515
0.0AspXaa: 0.0 ± 0.0
Glu
7.122GluAla: 7.122 ± 0.942
0.56GluCys: 0.56 ± 0.246
3.841GluAsp: 3.841 ± 0.958
3.761GluGlu: 3.761 ± 0.557
2.401GluPhe: 2.401 ± 0.461
4.081GluGly: 4.081 ± 0.538
1.761GluHis: 1.761 ± 0.408
2.721GluIle: 2.721 ± 0.506
3.121GluLys: 3.121 ± 0.623
5.682GluLeu: 5.682 ± 0.795
1.601GluMet: 1.601 ± 0.41
2.641GluAsn: 2.641 ± 0.502
1.761GluPro: 1.761 ± 0.498
3.121GluGln: 3.121 ± 0.672
2.321GluArg: 2.321 ± 0.558
2.561GluSer: 2.561 ± 0.466
2.801GluThr: 2.801 ± 0.482
4.161GluVal: 4.161 ± 0.683
0.88GluTrp: 0.88 ± 0.27
1.601GluTyr: 1.601 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
1.52PheAla: 1.52 ± 0.377
0.08PheCys: 0.08 ± 0.071
1.841PheAsp: 1.841 ± 0.345
2.481PheGlu: 2.481 ± 0.47
1.2PhePhe: 1.2 ± 0.27
2.321PheGly: 2.321 ± 0.398
0.72PheHis: 0.72 ± 0.336
1.28PheIle: 1.28 ± 0.346
2.321PheLys: 2.321 ± 0.467
2.881PheLeu: 2.881 ± 0.672
1.12PheMet: 1.12 ± 0.359
2.641PheAsn: 2.641 ± 0.542
1.04PhePro: 1.04 ± 0.297
0.96PheGln: 0.96 ± 0.335
1.12PheArg: 1.12 ± 0.32
2.401PheSer: 2.401 ± 0.506
2.321PheThr: 2.321 ± 0.51
2.241PheVal: 2.241 ± 0.372
0.4PheTrp: 0.4 ± 0.178
0.72PheTyr: 0.72 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
5.442GlyAla: 5.442 ± 1.0
0.4GlyCys: 0.4 ± 0.186
4.081GlyAsp: 4.081 ± 0.635
2.881GlyGlu: 2.881 ± 0.483
2.001GlyPhe: 2.001 ± 0.436
8.643GlyGly: 8.643 ± 2.095
0.64GlyHis: 0.64 ± 0.256
4.802GlyIle: 4.802 ± 0.487
5.682GlyLys: 5.682 ± 0.69
5.682GlyLeu: 5.682 ± 0.582
1.2GlyMet: 1.2 ± 0.31
5.282GlyAsn: 5.282 ± 0.804
1.841GlyPro: 1.841 ± 0.309
2.161GlyGln: 2.161 ± 0.277
3.281GlyArg: 3.281 ± 0.526
6.562GlySer: 6.562 ± 1.905
6.002GlyThr: 6.002 ± 0.745
6.162GlyVal: 6.162 ± 0.727
0.8GlyTrp: 0.8 ± 0.254
2.401GlyTyr: 2.401 ± 0.507
0.0GlyXaa: 0.0 ± 0.0
His
0.96HisAla: 0.96 ± 0.211
0.32HisCys: 0.32 ± 0.143
0.8HisAsp: 0.8 ± 0.269
0.8HisGlu: 0.8 ± 0.176
0.48HisPhe: 0.48 ± 0.243
1.12HisGly: 1.12 ± 0.257
0.56HisHis: 0.56 ± 0.219
1.12HisIle: 1.12 ± 0.301
0.72HisLys: 0.72 ± 0.19
1.12HisLeu: 1.12 ± 0.255
0.24HisMet: 0.24 ± 0.122
1.12HisAsn: 1.12 ± 0.266
1.28HisPro: 1.28 ± 0.274
0.4HisGln: 0.4 ± 0.164
0.96HisArg: 0.96 ± 0.261
0.56HisSer: 0.56 ± 0.275
1.44HisThr: 1.44 ± 0.372
1.2HisVal: 1.2 ± 0.369
0.24HisTrp: 0.24 ± 0.147
0.96HisTyr: 0.96 ± 0.319
0.0HisXaa: 0.0 ± 0.0
Ile
7.282IleAla: 7.282 ± 1.384
0.32IleCys: 0.32 ± 0.151
4.081IleAsp: 4.081 ± 0.71
2.721IleGlu: 2.721 ± 0.414
2.081IlePhe: 2.081 ± 0.312
3.201IleGly: 3.201 ± 0.461
1.04IleHis: 1.04 ± 0.258
2.401IleIle: 2.401 ± 0.438
4.722IleLys: 4.722 ± 0.586
3.281IleLeu: 3.281 ± 0.509
0.72IleMet: 0.72 ± 0.362
2.881IleAsn: 2.881 ± 0.428
2.881IlePro: 2.881 ± 0.601
2.961IleGln: 2.961 ± 0.389
2.161IleArg: 2.161 ± 0.358
4.802IleSer: 4.802 ± 0.556
4.802IleThr: 4.802 ± 1.017
2.961IleVal: 2.961 ± 0.382
0.64IleTrp: 0.64 ± 0.257
1.52IleTyr: 1.52 ± 0.399
0.0IleXaa: 0.0 ± 0.0
Lys
6.482LysAla: 6.482 ± 1.195
0.56LysCys: 0.56 ± 0.253
3.841LysAsp: 3.841 ± 0.707
3.601LysGlu: 3.601 ± 0.722
2.001LysPhe: 2.001 ± 0.395
4.081LysGly: 4.081 ± 0.61
1.2LysHis: 1.2 ± 0.302
5.762LysIle: 5.762 ± 1.568
5.442LysLys: 5.442 ± 0.965
6.482LysLeu: 6.482 ± 0.745
1.761LysMet: 1.761 ± 0.368
2.401LysAsn: 2.401 ± 0.515
2.961LysPro: 2.961 ± 0.589
2.561LysGln: 2.561 ± 0.562
2.721LysArg: 2.721 ± 0.457
2.881LysSer: 2.881 ± 0.626
3.041LysThr: 3.041 ± 0.59
3.121LysVal: 3.121 ± 0.763
0.96LysTrp: 0.96 ± 0.279
3.041LysTyr: 3.041 ± 0.678
0.0LysXaa: 0.0 ± 0.0
Leu
8.163LeuAla: 8.163 ± 0.836
0.64LeuCys: 0.64 ± 0.259
5.602LeuAsp: 5.602 ± 0.719
4.962LeuGlu: 4.962 ± 0.959
1.921LeuPhe: 1.921 ± 0.314
5.442LeuGly: 5.442 ± 0.787
1.2LeuHis: 1.2 ± 0.324
3.841LeuIle: 3.841 ± 0.685
5.922LeuLys: 5.922 ± 0.794
5.282LeuLeu: 5.282 ± 0.731
2.401LeuMet: 2.401 ± 0.474
4.161LeuAsn: 4.161 ± 0.521
3.201LeuPro: 3.201 ± 0.907
3.281LeuGln: 3.281 ± 0.575
3.441LeuArg: 3.441 ± 0.554
5.042LeuSer: 5.042 ± 0.53
4.081LeuThr: 4.081 ± 0.57
3.201LeuVal: 3.201 ± 0.54
0.8LeuTrp: 0.8 ± 0.216
2.321LeuTyr: 2.321 ± 0.517
0.0LeuXaa: 0.0 ± 0.0
Met
1.841MetAla: 1.841 ± 0.533
0.24MetCys: 0.24 ± 0.163
1.44MetAsp: 1.44 ± 0.367
1.841MetGlu: 1.841 ± 0.39
0.88MetPhe: 0.88 ± 0.282
1.681MetGly: 1.681 ± 0.392
0.48MetHis: 0.48 ± 0.249
1.601MetIle: 1.601 ± 0.394
1.681MetLys: 1.681 ± 0.38
1.52MetLeu: 1.52 ± 0.304
0.4MetMet: 0.4 ± 0.297
1.12MetAsn: 1.12 ± 0.341
0.64MetPro: 0.64 ± 0.233
1.12MetGln: 1.12 ± 0.373
1.28MetArg: 1.28 ± 0.378
1.52MetSer: 1.52 ± 0.303
2.641MetThr: 2.641 ± 0.431
1.12MetVal: 1.12 ± 0.367
0.24MetTrp: 0.24 ± 0.174
0.8MetTyr: 0.8 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
7.362AsnAla: 7.362 ± 1.429
0.32AsnCys: 0.32 ± 0.179
2.561AsnAsp: 2.561 ± 0.48
3.041AsnGlu: 3.041 ± 0.451
2.001AsnPhe: 2.001 ± 0.604
4.481AsnGly: 4.481 ± 0.632
1.12AsnHis: 1.12 ± 0.275
2.321AsnIle: 2.321 ± 0.426
3.121AsnLys: 3.121 ± 0.509
4.241AsnLeu: 4.241 ± 0.739
0.96AsnMet: 0.96 ± 0.343
4.802AsnAsn: 4.802 ± 1.217
2.881AsnPro: 2.881 ± 0.415
1.36AsnGln: 1.36 ± 0.321
2.721AsnArg: 2.721 ± 0.576
3.921AsnSer: 3.921 ± 0.673
3.281AsnThr: 3.281 ± 0.583
3.521AsnVal: 3.521 ± 0.681
0.88AsnTrp: 0.88 ± 0.241
2.481AsnTyr: 2.481 ± 0.494
0.0AsnXaa: 0.0 ± 0.0
Pro
3.441ProAla: 3.441 ± 0.536
0.24ProCys: 0.24 ± 0.149
2.481ProAsp: 2.481 ± 0.585
2.321ProGlu: 2.321 ± 0.56
1.761ProPhe: 1.761 ± 0.33
2.001ProGly: 2.001 ± 0.578
0.88ProHis: 0.88 ± 0.371
2.241ProIle: 2.241 ± 0.446
2.161ProLys: 2.161 ± 0.454
1.841ProLeu: 1.841 ± 0.38
1.12ProMet: 1.12 ± 0.414
3.041ProAsn: 3.041 ± 0.544
2.721ProPro: 2.721 ± 0.856
1.681ProGln: 1.681 ± 0.358
0.88ProArg: 0.88 ± 0.26
2.721ProSer: 2.721 ± 0.384
3.761ProThr: 3.761 ± 0.662
2.401ProVal: 2.401 ± 0.6
0.72ProTrp: 0.72 ± 0.253
1.36ProTyr: 1.36 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
4.161GlnAla: 4.161 ± 0.86
0.24GlnCys: 0.24 ± 0.147
2.081GlnAsp: 2.081 ± 0.41
1.681GlnGlu: 1.681 ± 0.377
1.44GlnPhe: 1.44 ± 0.369
3.201GlnGly: 3.201 ± 0.513
0.64GlnHis: 0.64 ± 0.173
2.561GlnIle: 2.561 ± 0.387
2.801GlnLys: 2.801 ± 0.641
3.361GlnLeu: 3.361 ± 0.641
1.04GlnMet: 1.04 ± 0.374
1.681GlnAsn: 1.681 ± 0.441
1.04GlnPro: 1.04 ± 0.304
2.721GlnGln: 2.721 ± 0.71
1.52GlnArg: 1.52 ± 0.342
2.961GlnSer: 2.961 ± 0.442
2.161GlnThr: 2.161 ± 0.347
2.641GlnVal: 2.641 ± 0.495
0.8GlnTrp: 0.8 ± 0.222
0.8GlnTyr: 0.8 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
3.361ArgAla: 3.361 ± 0.648
0.4ArgCys: 0.4 ± 0.181
2.961ArgAsp: 2.961 ± 0.66
2.961ArgGlu: 2.961 ± 0.633
1.28ArgPhe: 1.28 ± 0.334
1.841ArgGly: 1.841 ± 0.538
0.48ArgHis: 0.48 ± 0.219
2.161ArgIle: 2.161 ± 0.457
2.801ArgLys: 2.801 ± 0.722
3.361ArgLeu: 3.361 ± 0.493
1.36ArgMet: 1.36 ± 0.339
2.321ArgAsn: 2.321 ± 0.404
1.2ArgPro: 1.2 ± 0.329
1.52ArgGln: 1.52 ± 0.293
2.161ArgArg: 2.161 ± 0.374
2.161ArgSer: 2.161 ± 0.434
2.401ArgThr: 2.401 ± 0.415
2.401ArgVal: 2.401 ± 0.443
1.28ArgTrp: 1.28 ± 0.377
1.601ArgTyr: 1.601 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
5.202SerAla: 5.202 ± 0.794
0.72SerCys: 0.72 ± 0.231
4.321SerAsp: 4.321 ± 0.666
3.841SerGlu: 3.841 ± 0.383
2.641SerPhe: 2.641 ± 0.493
6.242SerGly: 6.242 ± 1.157
1.36SerHis: 1.36 ± 0.43
2.961SerIle: 2.961 ± 0.481
3.041SerLys: 3.041 ± 0.478
4.561SerLeu: 4.561 ± 0.674
1.681SerMet: 1.681 ± 0.407
4.401SerAsn: 4.401 ± 1.075
2.401SerPro: 2.401 ± 0.541
2.641SerGln: 2.641 ± 0.487
1.52SerArg: 1.52 ± 0.321
5.362SerSer: 5.362 ± 1.167
5.122SerThr: 5.122 ± 0.808
4.321SerVal: 4.321 ± 0.493
0.88SerTrp: 0.88 ± 0.273
2.721SerTyr: 2.721 ± 0.515
0.0SerXaa: 0.0 ± 0.0
Thr
9.763ThrAla: 9.763 ± 2.176
0.4ThrCys: 0.4 ± 0.174
4.161ThrAsp: 4.161 ± 0.673
4.401ThrGlu: 4.401 ± 0.563
1.681ThrPhe: 1.681 ± 0.425
6.562ThrGly: 6.562 ± 0.844
0.72ThrHis: 0.72 ± 0.228
4.241ThrIle: 4.241 ± 0.731
3.921ThrLys: 3.921 ± 0.471
4.722ThrLeu: 4.722 ± 0.522
1.601ThrMet: 1.601 ± 0.432
3.521ThrAsn: 3.521 ± 0.637
3.521ThrPro: 3.521 ± 0.678
2.561ThrGln: 2.561 ± 0.409
1.841ThrArg: 1.841 ± 0.303
5.202ThrSer: 5.202 ± 0.579
8.083ThrThr: 8.083 ± 2.121
4.802ThrVal: 4.802 ± 0.823
1.2ThrTrp: 1.2 ± 0.287
1.921ThrTyr: 1.921 ± 0.466
0.0ThrXaa: 0.0 ± 0.0
Val
4.401ValAla: 4.401 ± 0.676
0.72ValCys: 0.72 ± 0.234
4.401ValAsp: 4.401 ± 0.495
3.841ValGlu: 3.841 ± 0.552
1.601ValPhe: 1.601 ± 0.438
3.761ValGly: 3.761 ± 0.591
0.8ValHis: 0.8 ± 0.275
2.641ValIle: 2.641 ± 0.545
3.361ValLys: 3.361 ± 0.487
4.001ValLeu: 4.001 ± 0.766
1.681ValMet: 1.681 ± 0.323
3.361ValAsn: 3.361 ± 0.43
2.481ValPro: 2.481 ± 0.445
2.481ValGln: 2.481 ± 0.369
2.721ValArg: 2.721 ± 0.496
4.081ValSer: 4.081 ± 0.755
8.243ValThr: 8.243 ± 2.185
4.001ValVal: 4.001 ± 0.719
0.32ValTrp: 0.32 ± 0.145
1.921ValTyr: 1.921 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
0.96TrpAla: 0.96 ± 0.312
0.0TrpCys: 0.0 ± 0.0
1.04TrpAsp: 1.04 ± 0.268
1.28TrpGlu: 1.28 ± 0.365
0.4TrpPhe: 0.4 ± 0.154
0.88TrpGly: 0.88 ± 0.257
0.4TrpHis: 0.4 ± 0.218
0.8TrpIle: 0.8 ± 0.263
0.96TrpLys: 0.96 ± 0.252
0.88TrpLeu: 0.88 ± 0.278
0.4TrpMet: 0.4 ± 0.186
0.32TrpAsn: 0.32 ± 0.142
0.4TrpPro: 0.4 ± 0.208
0.64TrpGln: 0.64 ± 0.234
0.8TrpArg: 0.8 ± 0.296
0.4TrpSer: 0.4 ± 0.216
1.12TrpThr: 1.12 ± 0.26
0.88TrpVal: 0.88 ± 0.295
0.0TrpTrp: 0.0 ± 0.0
0.64TrpTyr: 0.64 ± 0.229
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.401TyrAla: 2.401 ± 0.548
0.4TyrCys: 0.4 ± 0.144
2.641TyrAsp: 2.641 ± 0.551
2.481TyrGlu: 2.481 ± 0.516
0.72TyrPhe: 0.72 ± 0.278
2.721TyrGly: 2.721 ± 0.546
0.4TyrHis: 0.4 ± 0.171
2.321TyrIle: 2.321 ± 0.371
2.481TyrLys: 2.481 ± 0.518
2.161TyrLeu: 2.161 ± 0.474
0.96TyrMet: 0.96 ± 0.228
1.841TyrAsn: 1.841 ± 0.313
1.36TyrPro: 1.36 ± 0.301
1.921TyrGln: 1.921 ± 0.473
1.921TyrArg: 1.921 ± 0.378
1.681TyrSer: 1.681 ± 0.367
1.921TyrThr: 1.921 ± 0.625
1.601TyrVal: 1.601 ± 0.425
0.32TyrTrp: 0.32 ± 0.174
1.28TyrTyr: 1.28 ± 0.402
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12497 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski