Trichophyton interdigitale (strain MR816)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8217 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A059JH20|A0A059JH20_TRIIM Aminopeptidase OS=Trichophyton interdigitale (strain MR816) OX=1215338 GN=H109_01429 PE=3 SV=1
MM1 pKa = 7.73 ADD3 pKa = 3.18 SVSIYY8 pKa = 10.87 DD9 pKa = 3.84 EE10 pKa = 4.41 IEE12 pKa = 4.35 IEE14 pKa = 5.66 DD15 pKa = 3.7 MTFDD19 pKa = 3.82 PVLQIYY25 pKa = 9.16 HH26 pKa = 5.73 YY27 pKa = 9.36 PCPCGDD33 pKa = 3.29 RR34 pKa = 11.84 FEE36 pKa = 6.13 IGLADD41 pKa = 3.99 LRR43 pKa = 11.84 DD44 pKa = 3.96 GEE46 pKa = 5.15 DD47 pKa = 2.8 IGVCPSCSLMIRR59 pKa = 11.84 VIFDD63 pKa = 3.33 EE64 pKa = 5.08 ADD66 pKa = 3.39 LPKK69 pKa = 10.6 DD70 pKa = 3.45 DD71 pKa = 4.72 SGNNGGVATAITAA84 pKa = 3.74
Molecular weight: 9.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.656
IPC_protein 3.617
Toseland 3.401
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.745
Patrickios 0.477
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.717
Protein with the highest isoelectric point:
>tr|A0A059JJK7|A0A059JJK7_TRIIM Uncharacterized protein OS=Trichophyton interdigitale (strain MR816) OX=1215338 GN=H109_00415 PE=4 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.41 SFRR8 pKa = 11.84 TKK10 pKa = 10.38 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 4.53
Molecular weight: 4.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7993
224
8217
4056786
36
7900
493.7
54.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.201 ± 0.023
1.218 ± 0.01
5.616 ± 0.021
6.295 ± 0.026
3.6 ± 0.018
6.66 ± 0.026
2.415 ± 0.011
5.132 ± 0.02
5.158 ± 0.021
8.773 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.009
3.836 ± 0.012
6.223 ± 0.032
4.135 ± 0.025
6.125 ± 0.023
8.744 ± 0.031
5.888 ± 0.016
5.822 ± 0.02
1.29 ± 0.011
2.734 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here