Lentinula edodes (Shiitake mushroom) (Lentinus edodes)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Omphalotaceae; Lentinula

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q3E836|A0A1Q3E836_LENED MICOS complex subunit MIC10 OS=Lentinula edodes OX=5353 GN=LENED_005046 PE=3 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84SHH4 pKa = 7.28IALLSVLVYY13 pKa = 10.66SAVLEE18 pKa = 4.24ANVLKK23 pKa = 10.59SKK25 pKa = 10.64RR26 pKa = 11.84VTEE29 pKa = 4.49ASCEE33 pKa = 4.08LTASSGDD40 pKa = 3.73DD41 pKa = 3.22APQFLAAAASCDD53 pKa = 3.77TVIIPAGTTLNIEE66 pKa = 4.22TRR68 pKa = 11.84LDD70 pKa = 3.44MTGMSNLNIDD80 pKa = 3.69LQGTIRR86 pKa = 11.84FNPDD90 pKa = 2.41LDD92 pKa = 3.76YY93 pKa = 11.09WSGNGFPITFQDD105 pKa = 5.15QITFWLLGGEE115 pKa = 4.33NIVLSGGGTLDD126 pKa = 3.45GAGQAWYY133 pKa = 9.62DD134 pKa = 3.56AFASNSSLLRR144 pKa = 11.84PIILTLNEE152 pKa = 3.95ATNVLVEE159 pKa = 5.22DD160 pKa = 3.55IQMINSPEE168 pKa = 3.74WFNLVNEE175 pKa = 4.55CQNVTFNNITITAKK189 pKa = 9.39STSDD193 pKa = 3.25NFISNTDD200 pKa = 2.44GWDD203 pKa = 2.91IYY205 pKa = 10.77RR206 pKa = 11.84SDD208 pKa = 3.43MVTIKK213 pKa = 10.94NSIINNGDD221 pKa = 3.25DD222 pKa = 3.6CVSFKK227 pKa = 11.02PNSTNILVSDD237 pKa = 4.28LNCNGSHH244 pKa = 6.91GISVGSLGQFSGVFDD259 pKa = 3.52IVQNVTAVNIQMANAEE275 pKa = 3.86NGARR279 pKa = 11.84IKK281 pKa = 10.93AFAGDD286 pKa = 4.0DD287 pKa = 3.74VGSGTVQNITFQNFVVSNVDD307 pKa = 3.39SPVVIDD313 pKa = 3.33QCYY316 pKa = 7.06EE317 pKa = 3.86TSADD321 pKa = 3.67DD322 pKa = 4.57CEE324 pKa = 4.36EE325 pKa = 4.03FPSNVFIQDD334 pKa = 2.81IFFNNISGTGSGSQVASLDD353 pKa = 3.58CSPGSRR359 pKa = 11.84CTDD362 pKa = 2.84INVNGFDD369 pKa = 5.14LSGSDD374 pKa = 3.18GKK376 pKa = 7.73TTYY379 pKa = 10.18EE380 pKa = 4.11CQNVVLAGNAASLFGTCTTTT400 pKa = 3.77

Molecular weight: 42.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q3EJC6|A0A1Q3EJC6_LENED 3'(2') 5'-bisphosphate nucleotidase OS=Lentinula edodes OX=5353 GN=LENED_009199 PE=3 SV=1
MM1 pKa = 6.88TVLWSTSGSPSSSMVAIASQQPRR24 pKa = 11.84PNTQAYY30 pKa = 9.76NNNTTNNPRR39 pKa = 11.84MILHH43 pKa = 7.14PIPRR47 pKa = 11.84LFFPAHH53 pKa = 5.78CVVDD57 pKa = 4.61RR58 pKa = 11.84SWGGSCSGEE67 pKa = 3.74RR68 pKa = 11.84GKK70 pKa = 10.47DD71 pKa = 2.87IFFQRR76 pKa = 11.84IEE78 pKa = 4.1VYY80 pKa = 10.05VEE82 pKa = 4.18KK83 pKa = 10.54KK84 pKa = 9.64RR85 pKa = 11.84KK86 pKa = 9.05RR87 pKa = 11.84PGRR90 pKa = 11.84FTLRR94 pKa = 11.84ANCHH98 pKa = 5.72ASAA101 pKa = 4.64

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12046

0

12046

5294775

58

4811

439.5

48.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.816 ± 0.019

1.145 ± 0.007

5.555 ± 0.016

6.044 ± 0.026

3.927 ± 0.014

6.353 ± 0.021

2.442 ± 0.009

5.332 ± 0.016

4.787 ± 0.023

9.199 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.009

3.957 ± 0.012

6.105 ± 0.024

3.719 ± 0.013

5.698 ± 0.018

9.293 ± 0.032

6.167 ± 0.016

6.227 ± 0.016

1.36 ± 0.009

2.785 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski