Tobacco virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1HRT1|A0A0K1HRT1_9CLOS Hsp70h OS=Tobacco virus 1 OX=1692045 GN=Hsp70h PE=4 SV=1
MM1 pKa = 7.41LSDD4 pKa = 4.21YY5 pKa = 10.96EE6 pKa = 4.37VGSNNSSHH14 pKa = 6.12YY15 pKa = 10.27LAIAVVCSEE24 pKa = 4.35NSDD27 pKa = 3.72EE28 pKa = 5.07LLMFEE33 pKa = 5.68SEE35 pKa = 4.72DD36 pKa = 3.74SNEE39 pKa = 3.74NAIFFEE45 pKa = 4.65DD46 pKa = 3.5SCVISKK52 pKa = 10.57SPSGGALNCAEE63 pKa = 4.25NYY65 pKa = 10.53YY66 pKa = 9.23LTSNAGLLDD75 pKa = 3.84FLKK78 pKa = 9.95STNFKK83 pKa = 10.48SPKK86 pKa = 8.36PLPVANRR93 pKa = 11.84CSNGPNHH100 pKa = 6.45VDD102 pKa = 3.46FHH104 pKa = 6.01MRR106 pKa = 11.84PLHH109 pKa = 6.26LGLIFRR115 pKa = 11.84VNLSLKK121 pKa = 10.76SLDD124 pKa = 5.13DD125 pKa = 4.5LDD127 pKa = 4.28TFQDD131 pKa = 3.61SLVKK135 pKa = 10.56IKK137 pKa = 10.67GFWNNGAQHH146 pKa = 6.17QSMFHH151 pKa = 5.64YY152 pKa = 10.52LSNFINYY159 pKa = 7.17EE160 pKa = 3.81KK161 pKa = 10.99YY162 pKa = 10.48SIVSFEE168 pKa = 3.93YY169 pKa = 10.38FKK171 pKa = 11.45LL172 pKa = 3.79

Molecular weight: 19.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1HSF4|A0A0K1HSF4_9CLOS CP OS=Tobacco virus 1 OX=1692045 GN=CP PE=4 SV=1
MM1 pKa = 7.76MITLISVFSTIRR13 pKa = 11.84SQAIPPRR20 pKa = 11.84SPSLQEE26 pKa = 3.55NLYY29 pKa = 10.6SFEE32 pKa = 3.87SRR34 pKa = 11.84NYY36 pKa = 10.77NFLTCEE42 pKa = 4.29RR43 pKa = 11.84YY44 pKa = 9.77SSPTVFGKK52 pKa = 10.73AMARR56 pKa = 11.84NLIEE60 pKa = 4.59RR61 pKa = 11.84CFTTDD66 pKa = 3.77KK67 pKa = 10.86FLEE70 pKa = 4.71FKK72 pKa = 10.61NSPIRR77 pKa = 11.84FSDD80 pKa = 3.6SALLKK85 pKa = 10.09WMQKK89 pKa = 9.78RR90 pKa = 11.84DD91 pKa = 3.35SSQIKK96 pKa = 9.9ALQSEE101 pKa = 4.79LTRR104 pKa = 11.84PLDD107 pKa = 3.56LSTAVHH113 pKa = 5.84YY114 pKa = 9.28FKK116 pKa = 11.44LMVKK120 pKa = 9.86RR121 pKa = 11.84DD122 pKa = 3.82AKK124 pKa = 11.02VKK126 pKa = 10.62LDD128 pKa = 3.62STCLTKK134 pKa = 10.64HH135 pKa = 5.98SAAQNIMFHH144 pKa = 6.39AKK146 pKa = 9.62AVNALYY152 pKa = 10.49SPCFDD157 pKa = 3.41EE158 pKa = 5.14LKK160 pKa = 10.98NRR162 pKa = 11.84FLSSLKK168 pKa = 9.6KK169 pKa = 9.95HH170 pKa = 5.64IVFFTEE176 pKa = 3.18MDD178 pKa = 3.03NRR180 pKa = 11.84TFARR184 pKa = 11.84VVNALVGSDD193 pKa = 4.85DD194 pKa = 3.85SDD196 pKa = 3.82LHH198 pKa = 7.57VGEE201 pKa = 4.56VDD203 pKa = 4.23FSKK206 pKa = 10.66FDD208 pKa = 3.29KK209 pKa = 11.13SQDD212 pKa = 2.91IFIKK216 pKa = 9.44EE217 pKa = 4.12FEE219 pKa = 4.35RR220 pKa = 11.84EE221 pKa = 4.23VYY223 pKa = 9.62TLLGFDD229 pKa = 4.62EE230 pKa = 5.56EE231 pKa = 4.99MLQLWMEE238 pKa = 4.4GEE240 pKa = 4.43YY241 pKa = 10.33SAKK244 pKa = 9.9ATTLDD249 pKa = 3.4GKK251 pKa = 11.2LSFEE255 pKa = 4.61VKK257 pKa = 8.97NQRR260 pKa = 11.84RR261 pKa = 11.84SGASNTWIGNSIVTLGILSMYY282 pKa = 10.23YY283 pKa = 10.31KK284 pKa = 10.43VDD286 pKa = 3.25EE287 pKa = 4.48LLALFVSGDD296 pKa = 3.63DD297 pKa = 3.5SLMYY301 pKa = 10.52SNKK304 pKa = 9.94PIANYY309 pKa = 10.47AEE311 pKa = 4.65SICVEE316 pKa = 4.2TGFEE320 pKa = 4.39TKK322 pKa = 10.0FLSPSVPYY330 pKa = 9.82FCSKK334 pKa = 10.44FVVHH338 pKa = 6.82CGFKK342 pKa = 10.3TFFVPDD348 pKa = 4.28PYY350 pKa = 11.75KK351 pKa = 11.22LMVKK355 pKa = 10.18LGSVRR360 pKa = 11.84KK361 pKa = 9.31EE362 pKa = 3.87LTDD365 pKa = 3.45KK366 pKa = 11.32DD367 pKa = 3.84LFEE370 pKa = 4.84VFTSFKK376 pKa = 11.11DD377 pKa = 3.51LTKK380 pKa = 10.96DD381 pKa = 3.27LGDD384 pKa = 3.49EE385 pKa = 4.13RR386 pKa = 11.84VLEE389 pKa = 4.03KK390 pKa = 11.24LNLLMIAKK398 pKa = 9.81YY399 pKa = 10.46GYY401 pKa = 10.54NSDD404 pKa = 3.66FALPALRR411 pKa = 11.84SIHH414 pKa = 6.36CLASNFSSFSKK425 pKa = 10.57LFEE428 pKa = 4.79KK429 pKa = 9.75STGWVVVPKK438 pKa = 10.72LNSYY442 pKa = 8.38YY443 pKa = 10.72KK444 pKa = 10.65KK445 pKa = 10.75LIALGAYY452 pKa = 8.16NEE454 pKa = 4.57RR455 pKa = 11.84YY456 pKa = 7.08VTPFGEE462 pKa = 4.57QYY464 pKa = 10.11FVAWW468 pKa = 3.73

Molecular weight: 53.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4936

65

2471

548.4

62.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.592 ± 0.267

2.431 ± 0.343

5.794 ± 0.156

6.22 ± 0.272

5.693 ± 0.356

4.862 ± 0.435

2.168 ± 0.238

5.369 ± 0.287

6.382 ± 0.328

9.542 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.133

4.781 ± 0.417

3.383 ± 0.195

2.208 ± 0.143

5.916 ± 0.59

10.008 ± 0.521

5.429 ± 0.367

7.78 ± 0.605

0.527 ± 0.141

3.89 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski