Tomato leaf curl Oman virus
Average proteome isoelectric point is 7.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1U825|E1U825_9GEMI C4 OS=Tomato leaf curl Oman virus OX=713949 GN=C4 PE=3 SV=1
MM1 pKa = 7.45 PRR3 pKa = 11.84 INSFCVNTKK12 pKa = 10.62 NIFLTYY18 pKa = 8.67 PKK20 pKa = 10.44 CPIPKK25 pKa = 9.32 EE26 pKa = 3.87 QMLEE30 pKa = 3.65 ILQSINCPSDD40 pKa = 3.09 KK41 pKa = 10.96 LFIRR45 pKa = 11.84 VSRR48 pKa = 11.84 EE49 pKa = 3.34 KK50 pKa = 10.76 HH51 pKa = 4.81 QDD53 pKa = 3.03 GSLHH57 pKa = 5.16 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.83 GKK67 pKa = 10.55 AKK69 pKa = 9.97 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 6.16 FDD77 pKa = 3.03 VTHH80 pKa = 7.17 PNNSSQFHH88 pKa = 7.01 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.24 SYY103 pKa = 10.82 IEE105 pKa = 4.35 KK106 pKa = 10.8 NGDD109 pKa = 3.67 YY110 pKa = 10.33 IDD112 pKa = 3.84 WGQFQIDD119 pKa = 3.44 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.92 ALNACSAEE144 pKa = 4.05 EE145 pKa = 3.76 ALSIIRR151 pKa = 11.84 EE152 pKa = 4.13 KK153 pKa = 10.9 LPKK156 pKa = 10.46 DD157 pKa = 4.77 FIFQYY162 pKa = 11.21 HH163 pKa = 5.5 NLKK166 pKa = 10.79 CNLDD170 pKa = 4.14 RR171 pKa = 11.84 IFQEE175 pKa = 4.33 PPAPYY180 pKa = 9.57 ISPFLSSSFNQVPEE194 pKa = 4.07 EE195 pKa = 4.2 LEE197 pKa = 4.05 VWVSEE202 pKa = 4.02 NVMSSAARR210 pKa = 11.84 PWRR213 pKa = 11.84 PNSIIIEE220 pKa = 4.33 GDD222 pKa = 3.13 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.2 VYY251 pKa = 9.81 STDD254 pKa = 2.18 AWYY257 pKa = 10.92 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.26 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 11.03 KK312 pKa = 10.55 EE313 pKa = 3.94 YY314 pKa = 10.94 LDD316 pKa = 3.59 EE317 pKa = 4.85 EE318 pKa = 4.69 KK319 pKa = 11.04 NISLKK324 pKa = 10.6 NWALKK329 pKa = 10.38 NATFVTLYY337 pKa = 10.65 EE338 pKa = 4.32 PLFSSANQNPTPHH351 pKa = 7.09 SEE353 pKa = 4.11 DD354 pKa = 3.12
Molecular weight: 40.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.557
IPC2_protein 6.605
IPC_protein 6.722
Toseland 6.781
ProMoST 6.985
Dawson 7.234
Bjellqvist 7.176
Wikipedia 7.205
Rodwell 7.234
Grimsley 6.898
Solomon 7.278
Lehninger 7.278
Nozaki 7.512
DTASelect 7.527
Thurlkill 7.571
EMBOSS 7.585
Sillero 7.658
Patrickios 4.202
IPC_peptide 7.278
IPC2_peptide 7.102
IPC2.peptide.svr19 7.005
Protein with the highest isoelectric point:
>tr|E1U822|E1U822_9GEMI Replication enhancer OS=Tomato leaf curl Oman virus OX=713949 PE=3 SV=1
MM1 pKa = 7.7 SKK3 pKa = 10.44 RR4 pKa = 11.84 PGDD7 pKa = 3.95 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.07 SPYY27 pKa = 10.0 SSRR30 pKa = 11.84 AAVPIVQGTNKK41 pKa = 9.27 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTYY47 pKa = 9.3 RR48 pKa = 11.84 PMYY51 pKa = 9.36 RR52 pKa = 11.84 KK53 pKa = 9.08 PRR55 pKa = 11.84 IYY57 pKa = 10.75 RR58 pKa = 11.84 MYY60 pKa = 10.32 RR61 pKa = 11.84 SPDD64 pKa = 3.21 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 9.32 VQSYY78 pKa = 6.99 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.05 DD83 pKa = 3.72 VKK85 pKa = 9.5 HH86 pKa = 5.48 TGIVRR91 pKa = 11.84 CVSDD95 pKa = 3.31 VTRR98 pKa = 11.84 GSGITHH104 pKa = 6.35 RR105 pKa = 11.84 VGKK108 pKa = 9.5 RR109 pKa = 11.84 FCVKK113 pKa = 10.37 SIYY116 pKa = 10.32 FLGKK120 pKa = 9.15 VWMDD124 pKa = 3.41 EE125 pKa = 4.15 NIKK128 pKa = 10.04 KK129 pKa = 9.97 QNHH132 pKa = 4.95 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.7 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.66 GSSPMDD153 pKa = 3.83 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.85 NDD173 pKa = 2.82 LRR175 pKa = 11.84 DD176 pKa = 3.36 RR177 pKa = 11.84 FQVMRR182 pKa = 11.84 KK183 pKa = 7.65 FHH185 pKa = 6.03 ATVIGGPSGMKK196 pKa = 9.5 EE197 pKa = 3.54 QALVKK202 pKa = 10.31 RR203 pKa = 11.84 FFRR206 pKa = 11.84 INSHH210 pKa = 3.89 VTYY213 pKa = 10.54 NHH215 pKa = 5.79 QEE217 pKa = 3.49 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 10.08 ATMKK247 pKa = 9.61 IRR249 pKa = 11.84 IYY251 pKa = 10.55 FYY253 pKa = 11.2 DD254 pKa = 4.06 SISNN258 pKa = 3.66
Molecular weight: 30.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.604
IPC_protein 9.97
Toseland 10.16
ProMoST 9.911
Dawson 10.379
Bjellqvist 10.087
Wikipedia 10.57
Rodwell 10.687
Grimsley 10.452
Solomon 10.409
Lehninger 10.365
Nozaki 10.175
DTASelect 10.072
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.189
IPC_peptide 10.409
IPC2_peptide 9.004
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1082
85
354
180.3
20.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.529 ± 0.744
2.218 ± 0.389
5.083 ± 0.348
4.621 ± 0.548
4.713 ± 0.51
4.344 ± 0.427
4.159 ± 0.785
6.839 ± 0.828
5.73 ± 0.644
6.007 ± 0.875
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.619
5.545 ± 0.7
6.47 ± 0.804
5.176 ± 0.631
7.024 ± 1.181
9.242 ± 1.402
4.991 ± 0.734
5.453 ± 1.059
1.294 ± 0.26
3.789 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here