Gracilimonas sp. 8A47

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Rhodohalobacter

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3239 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316TZG5|A0A316TZG5_9BACT Arginase OS=Gracilimonas sp. 8A47 OX=2079485 GN=DDZ15_12985 PE=3 SV=1
MM1 pKa = 7.34KK2 pKa = 9.96RR3 pKa = 11.84YY4 pKa = 10.29SILTLLIFSTAVLMHH19 pKa = 6.81GCTDD23 pKa = 4.62DD24 pKa = 5.16GPLGPDD30 pKa = 3.46PDD32 pKa = 4.59DD33 pKa = 4.53FDD35 pKa = 5.66RR36 pKa = 11.84SAILTNWADD45 pKa = 3.67NLIVPAYY52 pKa = 9.93QNFVADD58 pKa = 4.57AEE60 pKa = 4.38ALEE63 pKa = 4.4SAAADD68 pKa = 4.83FSANPAADD76 pKa = 3.6EE77 pKa = 4.27LLALRR82 pKa = 11.84NAFEE86 pKa = 3.9TAYY89 pKa = 11.11LSFQKK94 pKa = 10.81VSMYY98 pKa = 10.52EE99 pKa = 3.64IGPAMQVGAQGINLRR114 pKa = 11.84NYY116 pKa = 9.66LNSYY120 pKa = 10.01PSDD123 pKa = 3.21TAQIVEE129 pKa = 4.36NVSLNDD135 pKa = 3.42INLDD139 pKa = 4.03LPSQLDD145 pKa = 3.69AQGFPALDD153 pKa = 3.49YY154 pKa = 11.07LLYY157 pKa = 10.69GAAGSEE163 pKa = 4.19AEE165 pKa = 4.13ILALYY170 pKa = 10.51SEE172 pKa = 5.16GADD175 pKa = 3.16APLYY179 pKa = 10.32RR180 pKa = 11.84SYY182 pKa = 11.31LQTLSARR189 pKa = 11.84IAGLAGNVLNAWEE202 pKa = 4.07TGYY205 pKa = 10.88RR206 pKa = 11.84DD207 pKa = 3.67EE208 pKa = 4.98FTDD211 pKa = 4.1NSGNGANASIDD222 pKa = 3.57MMVNDD227 pKa = 4.22YY228 pKa = 10.97IFYY231 pKa = 8.77YY232 pKa = 9.89EE233 pKa = 3.5KK234 pKa = 9.89WLRR237 pKa = 11.84AGKK240 pKa = 10.23VGIPAGVFSGTPLPTHH256 pKa = 6.83VEE258 pKa = 3.9ALYY261 pKa = 10.48HH262 pKa = 6.33GSFSRR267 pKa = 11.84QLALEE272 pKa = 4.32ALSAVQDD279 pKa = 4.04FFNGNHH285 pKa = 5.28VNGTGSGEE293 pKa = 4.28SLNSYY298 pKa = 10.75LDD300 pKa = 3.78YY301 pKa = 11.08LDD303 pKa = 3.79SRR305 pKa = 11.84RR306 pKa = 11.84DD307 pKa = 3.55GSQLSVLINSQFEE320 pKa = 4.32SARR323 pKa = 11.84SEE325 pKa = 4.7LEE327 pKa = 3.84SLQQNFADD335 pKa = 4.05QVEE338 pKa = 4.24NDD340 pKa = 4.06NIRR343 pKa = 11.84MLNAYY348 pKa = 9.96DD349 pKa = 4.03EE350 pKa = 4.38LQKK353 pKa = 11.17NVVFMKK359 pKa = 10.61VDD361 pKa = 3.29MLQALNISVDD371 pKa = 3.76YY372 pKa = 11.37VDD374 pKa = 5.38ADD376 pKa = 3.45GDD378 pKa = 3.83

Molecular weight: 41.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316TVQ0|A0A316TVQ0_9BACT Uncharacterized protein OS=Gracilimonas sp. 8A47 OX=2079485 GN=DDZ15_08730 PE=4 SV=1
MM1 pKa = 7.34WKK3 pKa = 9.8SVPRR7 pKa = 11.84FRR9 pKa = 11.84QSILVLGGLFLTLIWLIILSVGIGAVPISPSQVISIFLNQAGIEE53 pKa = 4.09TGLGYY58 pKa = 10.78EE59 pKa = 4.54SVQEE63 pKa = 4.26SILLNIRR70 pKa = 11.84LPRR73 pKa = 11.84VLLAVLVGAALAISGAAMQGLFRR96 pKa = 11.84NPLADD101 pKa = 4.27PGLIGVSSGAALATAIAMVVLSSLAWPFLDD131 pKa = 5.03LLGEE135 pKa = 4.1ALIPLAAFAGGTSATILVYY154 pKa = 10.49RR155 pKa = 11.84LSTSRR160 pKa = 11.84GRR162 pKa = 11.84TNVATMLLAGIAINAMAGALIGFMIFLADD191 pKa = 4.26DD192 pKa = 3.95DD193 pKa = 4.13QLRR196 pKa = 11.84DD197 pKa = 3.57LTFWTLGSLGGAMWKK212 pKa = 10.08SVWIVLPILMAAILFLPRR230 pKa = 11.84LSKK233 pKa = 10.75GLNAILLGEE242 pKa = 4.25AEE244 pKa = 4.1ARR246 pKa = 11.84HH247 pKa = 5.85LGVRR251 pKa = 11.84VQRR254 pKa = 11.84LKK256 pKa = 10.78KK257 pKa = 10.47VIIIFVGLAVGAAVSVSGMIGFVGLVVPHH286 pKa = 7.34ILRR289 pKa = 11.84LWIGPDD295 pKa = 3.23HH296 pKa = 7.05RR297 pKa = 11.84FLMPGSAILGGLLLLASDD315 pKa = 4.43LAARR319 pKa = 11.84TMVAPAEE326 pKa = 4.07LPIGVITASIGAPFFLWLLLRR347 pKa = 11.84NRR349 pKa = 11.84EE350 pKa = 3.98LRR352 pKa = 11.84GYY354 pKa = 10.24LL355 pKa = 3.48

Molecular weight: 37.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3239

0

3239

1132565

38

4770

349.7

39.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.192 ± 0.05

0.607 ± 0.012

6.016 ± 0.034

7.393 ± 0.051

4.695 ± 0.031

7.283 ± 0.039

1.965 ± 0.023

6.845 ± 0.041

4.74 ± 0.052

9.522 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.023

4.528 ± 0.035

4.142 ± 0.023

3.424 ± 0.025

5.4 ± 0.038

6.967 ± 0.039

5.521 ± 0.05

6.482 ± 0.035

1.232 ± 0.016

3.496 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski