Human feces pecovirus
Average proteome isoelectric point is 8.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142J7H6|A0A142J7H6_9VIRU Replication associated protein OS=Human feces pecovirus OX=1820160 PE=4 SV=1
MM1 pKa = 8.1 SMRR4 pKa = 11.84 TRR6 pKa = 11.84 NWCVTSWTCPDD17 pKa = 3.48 YY18 pKa = 11.54 DD19 pKa = 3.69 SVEE22 pKa = 4.1 RR23 pKa = 11.84 LVVSGEE29 pKa = 3.88 YY30 pKa = 10.34 KK31 pKa = 9.82 YY32 pKa = 11.36 VCVGKK37 pKa = 7.87 EE38 pKa = 3.72 TCPRR42 pKa = 11.84 TGKK45 pKa = 8.4 VHH47 pKa = 4.87 WHH49 pKa = 6.83 WYY51 pKa = 7.75 LQAVNKK57 pKa = 10.5 KK58 pKa = 8.55 SMRR61 pKa = 11.84 QIKK64 pKa = 10.17 KK65 pKa = 10.46 DD66 pKa = 3.21 VGDD69 pKa = 4.03 RR70 pKa = 11.84 MAHH73 pKa = 6.13 AEE75 pKa = 4.09 AANGPADD82 pKa = 3.98 AGIAYY87 pKa = 9.08 VKK89 pKa = 10.61 KK90 pKa = 10.5 DD91 pKa = 3.85 GEE93 pKa = 4.18 WKK95 pKa = 10.18 EE96 pKa = 3.61 WGEE99 pKa = 3.88 PKK101 pKa = 10.26 KK102 pKa = 10.53 QGRR105 pKa = 11.84 RR106 pKa = 11.84 SDD108 pKa = 5.02 LEE110 pKa = 4.14 DD111 pKa = 3.49 CKK113 pKa = 11.07 KK114 pKa = 10.79 IIDD117 pKa = 4.05 NSVDD121 pKa = 3.46 DD122 pKa = 5.64 AMGTMQQIADD132 pKa = 3.6 THH134 pKa = 6.26 FGDD137 pKa = 4.54 FVRR140 pKa = 11.84 YY141 pKa = 8.38 YY142 pKa = 10.81 RR143 pKa = 11.84 GFAKK147 pKa = 10.45 YY148 pKa = 11.04 ADD150 pKa = 3.54 MVDD153 pKa = 3.2 IKK155 pKa = 10.43 RR156 pKa = 11.84 AKK158 pKa = 9.3 QRR160 pKa = 11.84 EE161 pKa = 3.95 PHH163 pKa = 5.31 MPEE166 pKa = 3.66 VIVYY170 pKa = 9.46 LGRR173 pKa = 11.84 AGSGKK178 pKa = 10.23 SYY180 pKa = 10.44 HH181 pKa = 6.94 CYY183 pKa = 9.89 HH184 pKa = 7.49 DD185 pKa = 3.73 EE186 pKa = 4.63 GYY188 pKa = 10.22 QRR190 pKa = 11.84 SGYY193 pKa = 9.76 RR194 pKa = 11.84 YY195 pKa = 8.83 LVQMEE200 pKa = 4.56 SKK202 pKa = 9.53 TYY204 pKa = 9.65 FDD206 pKa = 5.07 GYY208 pKa = 10.85 DD209 pKa = 3.49 GEE211 pKa = 4.34 KK212 pKa = 10.21 TIWFDD217 pKa = 3.52 EE218 pKa = 4.47 FSGRR222 pKa = 11.84 VMSFTVFTQIVDD234 pKa = 3.28 QWGSRR239 pKa = 11.84 VEE241 pKa = 4.24 TKK243 pKa = 10.55 GGSRR247 pKa = 11.84 QIFPEE252 pKa = 4.1 RR253 pKa = 11.84 FLISTVEE260 pKa = 4.2 WPSQWWAGSSRR271 pKa = 11.84 FNADD275 pKa = 2.9 PYY277 pKa = 10.24 QLYY280 pKa = 10.41 RR281 pKa = 11.84 RR282 pKa = 11.84 ITKK285 pKa = 9.81 IYY287 pKa = 7.71 WCRR290 pKa = 11.84 GPGQEE295 pKa = 4.43 PVEE298 pKa = 4.14 LKK300 pKa = 10.62 KK301 pKa = 10.96 DD302 pKa = 2.85 GWTCKK307 pKa = 10.78 NIDD310 pKa = 3.73 EE311 pKa = 4.34 YY312 pKa = 11.62 EE313 pKa = 4.1 EE314 pKa = 5.01 LFGKK318 pKa = 10.62 LPP320 pKa = 3.67
Molecular weight: 37.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.187
IPC2_protein 7.556
IPC_protein 7.439
Toseland 7.088
ProMoST 8.053
Dawson 8.141
Bjellqvist 8.448
Wikipedia 8.068
Rodwell 8.156
Grimsley 7.117
Solomon 8.214
Lehninger 8.229
Nozaki 8.595
DTASelect 8.214
Thurlkill 8.273
EMBOSS 8.346
Sillero 8.58
Patrickios 4.164
IPC_peptide 8.214
IPC2_peptide 7.424
IPC2.peptide.svr19 7.634
Protein with the highest isoelectric point:
>tr|A0A142J7H6|A0A142J7H6_9VIRU Replication associated protein OS=Human feces pecovirus OX=1820160 PE=4 SV=1
MM1 pKa = 7.12 SHH3 pKa = 6.93 LVSHH7 pKa = 6.82 INVMARR13 pKa = 11.84 RR14 pKa = 11.84 YY15 pKa = 8.75 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 FGRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 PRR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 9.0 LRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 ALLRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 LLRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 GGRR51 pKa = 11.84 RR52 pKa = 11.84 FTVSVQRR59 pKa = 11.84 DD60 pKa = 3.58 FALIAASRR68 pKa = 11.84 VPEE71 pKa = 4.05 ATQLAGNGTNVAGPGGVVGIDD92 pKa = 2.95 TGGFRR97 pKa = 11.84 SYY99 pKa = 11.36 SVLALPPDD107 pKa = 3.95 WIEE110 pKa = 3.82 RR111 pKa = 11.84 TVGDD115 pKa = 3.64 TRR117 pKa = 11.84 LVGNQWDD124 pKa = 3.75 VSNKK128 pKa = 9.57 YY129 pKa = 9.05 LQRR132 pKa = 11.84 CIGVGTGEE140 pKa = 4.27 SGMLTDD146 pKa = 5.27 LPQIIWSKK154 pKa = 9.66 PLPVNFNCGSLWDD167 pKa = 3.74 LCEE170 pKa = 4.08 EE171 pKa = 4.54 YY172 pKa = 10.5 RR173 pKa = 11.84 IAWIALSFTVAEE185 pKa = 4.42 SQTKK189 pKa = 9.42 NNRR192 pKa = 11.84 HH193 pKa = 6.43 LYY195 pKa = 10.49 LEE197 pKa = 4.33 WTNLPSAKK205 pKa = 10.19 APQWTDD211 pKa = 2.63 LDD213 pKa = 4.29 GMVLPEE219 pKa = 4.98 GYY221 pKa = 10.5 AGGGTNVDD229 pKa = 3.4 TNGFNWLCRR238 pKa = 11.84 PIDD241 pKa = 3.47 IALACSPSGRR251 pKa = 11.84 QSKK254 pKa = 10.44 LNGWHH259 pKa = 7.06 RR260 pKa = 11.84 SILSYY265 pKa = 7.76 THH267 pKa = 7.09 PVTIRR272 pKa = 11.84 YY273 pKa = 8.46 RR274 pKa = 11.84 PRR276 pKa = 11.84 HH277 pKa = 5.85 ADD279 pKa = 3.08 MTIDD283 pKa = 3.83 NNPDD287 pKa = 3.19 VAPDD291 pKa = 3.52 SSNQNKK297 pKa = 9.65 PKK299 pKa = 10.04 MIYY302 pKa = 9.88 SDD304 pKa = 3.61 NFSEE308 pKa = 4.62 SGRR311 pKa = 11.84 LTRR314 pKa = 11.84 GYY316 pKa = 10.74 LPVAQASVLLTEE328 pKa = 4.42 QCWFGPCIRR337 pKa = 11.84 VVDD340 pKa = 4.07 ADD342 pKa = 3.9 KK343 pKa = 10.91 TPQLGTNEE351 pKa = 3.76 QAFNSFYY358 pKa = 10.86 DD359 pKa = 3.73 EE360 pKa = 4.1 YY361 pKa = 11.35 GIRR364 pKa = 11.84 CSMTAVLRR372 pKa = 11.84 MRR374 pKa = 11.84 AKK376 pKa = 9.26 TCNDD380 pKa = 3.14 PVFPYY385 pKa = 9.41 YY386 pKa = 9.79 TVHH389 pKa = 7.07
Molecular weight: 44.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.098
IPC2_protein 9.545
IPC_protein 10.394
Toseland 10.043
ProMoST 9.97
Dawson 10.335
Bjellqvist 10.145
Wikipedia 10.599
Rodwell 10.379
Grimsley 10.438
Solomon 10.394
Lehninger 10.335
Nozaki 10.16
DTASelect 10.116
Thurlkill 10.175
EMBOSS 10.496
Sillero 10.277
Patrickios 7.585
IPC_peptide 10.379
IPC2_peptide 9.428
IPC2.peptide.svr19 8.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
709
320
389
354.5
40.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.783 ± 0.516
2.398 ± 0.067
6.065 ± 0.739
4.795 ± 1.37
3.385 ± 0.24
8.322 ± 0.282
1.975 ± 0.14
4.372 ± 0.002
4.937 ± 2.1
6.065 ± 1.936
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.386
3.949 ± 1.366
4.937 ± 0.782
3.808 ± 0.373
9.873 ± 1.769
6.347 ± 0.27
5.501 ± 0.536
6.77 ± 0.069
3.244 ± 0.539
4.937 ± 0.865
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here