Amino acid dipepetide frequency for Burkholderia phage Bcep176

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.508AlaAla: 23.508 ± 3.503
1.126AlaCys: 1.126 ± 0.284
7.812AlaAsp: 7.812 ± 0.678
8.657AlaGlu: 8.657 ± 1.012
3.59AlaPhe: 3.59 ± 0.477
12.176AlaGly: 12.176 ± 0.992
2.463AlaHis: 2.463 ± 0.389
6.264AlaIle: 6.264 ± 0.581
4.786AlaLys: 4.786 ± 0.497
11.332AlaLeu: 11.332 ± 1.187
4.082AlaMet: 4.082 ± 0.535
4.082AlaAsn: 4.082 ± 0.586
5.349AlaPro: 5.349 ± 0.765
5.701AlaGln: 5.701 ± 0.72
10.698AlaArg: 10.698 ± 1.21
7.531AlaSer: 7.531 ± 1.039
5.49AlaThr: 5.49 ± 0.846
7.038AlaVal: 7.038 ± 0.819
2.182AlaTrp: 2.182 ± 0.51
2.956AlaTyr: 2.956 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.915CysAla: 0.915 ± 0.294
0.352CysCys: 0.352 ± 0.174
0.422CysAsp: 0.422 ± 0.177
0.845CysGlu: 0.845 ± 0.272
0.211CysPhe: 0.211 ± 0.131
0.845CysGly: 0.845 ± 0.258
0.141CysHis: 0.141 ± 0.12
0.141CysIle: 0.141 ± 0.094
0.211CysLys: 0.211 ± 0.118
0.704CysLeu: 0.704 ± 0.198
0.07CysMet: 0.07 ± 0.081
0.211CysAsn: 0.211 ± 0.113
0.774CysPro: 0.774 ± 0.247
0.211CysGln: 0.211 ± 0.117
0.845CysArg: 0.845 ± 0.281
1.056CysSer: 1.056 ± 0.299
0.422CysThr: 0.422 ± 0.156
0.774CysVal: 0.774 ± 0.24
0.352CysTrp: 0.352 ± 0.126
0.352CysTyr: 0.352 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
10.276AspAla: 10.276 ± 1.094
0.493AspCys: 0.493 ± 0.201
4.364AspAsp: 4.364 ± 0.594
3.449AspGlu: 3.449 ± 0.437
1.337AspPhe: 1.337 ± 0.296
6.827AspGly: 6.827 ± 0.948
0.845AspHis: 0.845 ± 0.22
1.9AspIle: 1.9 ± 0.323
1.548AspLys: 1.548 ± 0.275
4.153AspLeu: 4.153 ± 0.529
1.689AspMet: 1.689 ± 0.316
1.619AspAsn: 1.619 ± 0.294
2.534AspPro: 2.534 ± 0.545
2.534AspGln: 2.534 ± 0.478
3.66AspArg: 3.66 ± 0.432
2.956AspSer: 2.956 ± 0.393
2.463AspThr: 2.463 ± 0.353
4.012AspVal: 4.012 ± 0.603
1.548AspTrp: 1.548 ± 0.309
1.197AspTyr: 1.197 ± 0.237
0.0AspXaa: 0.0 ± 0.0
Glu
6.546GluAla: 6.546 ± 0.818
0.563GluCys: 0.563 ± 0.23
2.675GluAsp: 2.675 ± 0.431
3.167GluGlu: 3.167 ± 0.417
2.815GluPhe: 2.815 ± 0.511
3.59GluGly: 3.59 ± 0.436
1.267GluHis: 1.267 ± 0.368
3.59GluIle: 3.59 ± 0.466
2.815GluLys: 2.815 ± 0.57
3.801GluLeu: 3.801 ± 0.693
1.337GluMet: 1.337 ± 0.299
1.197GluAsn: 1.197 ± 0.374
3.026GluPro: 3.026 ± 0.461
3.097GluGln: 3.097 ± 0.548
5.349GluArg: 5.349 ± 0.657
3.097GluSer: 3.097 ± 0.389
2.745GluThr: 2.745 ± 0.395
3.097GluVal: 3.097 ± 0.509
1.83GluTrp: 1.83 ± 0.37
1.83GluTyr: 1.83 ± 0.261
0.0GluXaa: 0.0 ± 0.0
Phe
3.73PheAla: 3.73 ± 0.508
0.211PheCys: 0.211 ± 0.126
2.393PheAsp: 2.393 ± 0.406
1.83PheGlu: 1.83 ± 0.388
1.126PhePhe: 1.126 ± 0.294
2.956PheGly: 2.956 ± 0.355
0.633PheHis: 0.633 ± 0.175
0.774PheIle: 0.774 ± 0.195
1.267PheLys: 1.267 ± 0.317
2.323PheLeu: 2.323 ± 0.433
1.126PheMet: 1.126 ± 0.294
1.197PheAsn: 1.197 ± 0.285
0.845PhePro: 0.845 ± 0.297
1.197PheGln: 1.197 ± 0.275
1.971PheArg: 1.971 ± 0.338
1.337PheSer: 1.337 ± 0.265
1.548PheThr: 1.548 ± 0.242
2.675PheVal: 2.675 ± 0.543
0.493PheTrp: 0.493 ± 0.171
0.915PheTyr: 0.915 ± 0.235
0.0PheXaa: 0.0 ± 0.0
Gly
8.024GlyAla: 8.024 ± 0.777
0.633GlyCys: 0.633 ± 0.216
5.208GlyAsp: 5.208 ± 0.617
4.223GlyGlu: 4.223 ± 0.53
2.252GlyPhe: 2.252 ± 0.379
8.164GlyGly: 8.164 ± 0.9
1.056GlyHis: 1.056 ± 0.22
4.716GlyIle: 4.716 ± 0.574
3.73GlyLys: 3.73 ± 0.526
5.771GlyLeu: 5.771 ± 0.693
2.534GlyMet: 2.534 ± 0.432
2.886GlyAsn: 2.886 ± 0.436
2.463GlyPro: 2.463 ± 0.402
3.66GlyGln: 3.66 ± 0.411
6.264GlyArg: 6.264 ± 0.595
5.068GlySer: 5.068 ± 0.609
4.505GlyThr: 4.505 ± 0.562
6.123GlyVal: 6.123 ± 0.65
1.9GlyTrp: 1.9 ± 0.353
2.463GlyTyr: 2.463 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.83HisAla: 1.83 ± 0.446
0.07HisCys: 0.07 ± 0.065
1.478HisAsp: 1.478 ± 0.291
0.704HisGlu: 0.704 ± 0.163
0.563HisPhe: 0.563 ± 0.224
1.056HisGly: 1.056 ± 0.305
0.141HisHis: 0.141 ± 0.098
0.352HisIle: 0.352 ± 0.15
0.704HisLys: 0.704 ± 0.245
1.619HisLeu: 1.619 ± 0.327
0.422HisMet: 0.422 ± 0.174
0.141HisAsn: 0.141 ± 0.097
0.985HisPro: 0.985 ± 0.365
0.633HisGln: 0.633 ± 0.171
1.83HisArg: 1.83 ± 0.299
0.915HisSer: 0.915 ± 0.241
0.493HisThr: 0.493 ± 0.178
1.408HisVal: 1.408 ± 0.308
0.563HisTrp: 0.563 ± 0.174
0.352HisTyr: 0.352 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
7.531IleAla: 7.531 ± 0.834
0.422IleCys: 0.422 ± 0.177
4.293IleAsp: 4.293 ± 0.465
3.026IleGlu: 3.026 ± 0.397
0.633IlePhe: 0.633 ± 0.191
4.223IleGly: 4.223 ± 0.484
0.563IleHis: 0.563 ± 0.156
1.83IleIle: 1.83 ± 0.432
1.478IleLys: 1.478 ± 0.246
2.745IleLeu: 2.745 ± 0.479
0.282IleMet: 0.282 ± 0.156
1.619IleAsn: 1.619 ± 0.313
2.393IlePro: 2.393 ± 0.37
0.985IleGln: 0.985 ± 0.348
3.801IleArg: 3.801 ± 0.468
2.886IleSer: 2.886 ± 0.414
3.097IleThr: 3.097 ± 0.573
4.364IleVal: 4.364 ± 0.536
0.422IleTrp: 0.422 ± 0.212
0.915IleTyr: 0.915 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
6.053LysAla: 6.053 ± 0.635
0.141LysCys: 0.141 ± 0.1
1.478LysAsp: 1.478 ± 0.265
1.971LysGlu: 1.971 ± 0.433
1.337LysPhe: 1.337 ± 0.26
2.675LysGly: 2.675 ± 0.397
0.915LysHis: 0.915 ± 0.309
1.689LysIle: 1.689 ± 0.347
2.041LysLys: 2.041 ± 0.434
3.801LysLeu: 3.801 ± 0.622
1.197LysMet: 1.197 ± 0.327
0.985LysAsn: 0.985 ± 0.257
2.182LysPro: 2.182 ± 0.41
2.463LysGln: 2.463 ± 0.414
3.238LysArg: 3.238 ± 0.492
2.886LysSer: 2.886 ± 0.398
2.111LysThr: 2.111 ± 0.512
2.886LysVal: 2.886 ± 0.499
0.774LysTrp: 0.774 ± 0.249
0.915LysTyr: 0.915 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
9.994LeuAla: 9.994 ± 1.435
0.563LeuCys: 0.563 ± 0.209
5.068LeuAsp: 5.068 ± 0.539
5.068LeuGlu: 5.068 ± 0.576
2.111LeuPhe: 2.111 ± 0.417
5.419LeuGly: 5.419 ± 0.663
1.267LeuHis: 1.267 ± 0.29
4.293LeuIle: 4.293 ± 0.542
3.238LeuLys: 3.238 ± 0.577
5.631LeuLeu: 5.631 ± 0.6
1.689LeuMet: 1.689 ± 0.284
2.604LeuAsn: 2.604 ± 0.487
3.941LeuPro: 3.941 ± 0.465
2.252LeuGln: 2.252 ± 0.435
6.405LeuArg: 6.405 ± 0.79
4.364LeuSer: 4.364 ± 0.614
5.49LeuThr: 5.49 ± 0.543
4.223LeuVal: 4.223 ± 0.625
0.352LeuTrp: 0.352 ± 0.148
2.182LeuTyr: 2.182 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
3.801MetAla: 3.801 ± 0.544
0.352MetCys: 0.352 ± 0.193
1.197MetAsp: 1.197 ± 0.259
0.915MetGlu: 0.915 ± 0.242
0.563MetPhe: 0.563 ± 0.242
1.408MetGly: 1.408 ± 0.28
0.141MetHis: 0.141 ± 0.105
1.126MetIle: 1.126 ± 0.291
1.548MetLys: 1.548 ± 0.299
2.675MetLeu: 2.675 ± 0.482
0.563MetMet: 0.563 ± 0.168
1.408MetAsn: 1.408 ± 0.245
1.408MetPro: 1.408 ± 0.396
1.197MetGln: 1.197 ± 0.266
1.619MetArg: 1.619 ± 0.323
2.111MetSer: 2.111 ± 0.447
1.83MetThr: 1.83 ± 0.331
0.915MetVal: 0.915 ± 0.252
0.282MetTrp: 0.282 ± 0.14
0.422MetTyr: 0.422 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
4.364AsnAla: 4.364 ± 0.594
0.422AsnCys: 0.422 ± 0.171
1.619AsnAsp: 1.619 ± 0.337
1.548AsnGlu: 1.548 ± 0.275
0.985AsnPhe: 0.985 ± 0.246
3.238AsnGly: 3.238 ± 0.46
0.422AsnHis: 0.422 ± 0.188
1.619AsnIle: 1.619 ± 0.256
1.408AsnLys: 1.408 ± 0.337
2.252AsnLeu: 2.252 ± 0.367
0.704AsnMet: 0.704 ± 0.197
1.126AsnAsn: 1.126 ± 0.31
1.619AsnPro: 1.619 ± 0.409
0.563AsnGln: 0.563 ± 0.165
2.604AsnArg: 2.604 ± 0.437
1.9AsnSer: 1.9 ± 0.463
1.267AsnThr: 1.267 ± 0.256
2.323AsnVal: 2.323 ± 0.537
0.422AsnTrp: 0.422 ± 0.132
0.845AsnTyr: 0.845 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
5.983ProAla: 5.983 ± 0.818
0.704ProCys: 0.704 ± 0.223
2.675ProAsp: 2.675 ± 0.479
3.167ProGlu: 3.167 ± 0.414
1.408ProPhe: 1.408 ± 0.337
4.082ProGly: 4.082 ± 0.55
0.845ProHis: 0.845 ± 0.232
2.182ProIle: 2.182 ± 0.346
1.76ProLys: 1.76 ± 0.371
3.449ProLeu: 3.449 ± 0.504
1.83ProMet: 1.83 ± 0.36
1.9ProAsn: 1.9 ± 0.404
2.815ProPro: 2.815 ± 0.591
0.845ProGln: 0.845 ± 0.252
2.815ProArg: 2.815 ± 0.502
2.956ProSer: 2.956 ± 0.61
3.308ProThr: 3.308 ± 0.571
2.956ProVal: 2.956 ± 0.567
0.774ProTrp: 0.774 ± 0.239
0.915ProTyr: 0.915 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
4.997GlnAla: 4.997 ± 0.686
0.352GlnCys: 0.352 ± 0.147
1.408GlnAsp: 1.408 ± 0.295
1.056GlnGlu: 1.056 ± 0.266
1.478GlnPhe: 1.478 ± 0.28
2.534GlnGly: 2.534 ± 0.491
0.141GlnHis: 0.141 ± 0.091
2.252GlnIle: 2.252 ± 0.518
1.76GlnLys: 1.76 ± 0.349
3.167GlnLeu: 3.167 ± 0.587
1.197GlnMet: 1.197 ± 0.29
1.267GlnAsn: 1.267 ± 0.3
3.026GlnPro: 3.026 ± 0.62
1.478GlnGln: 1.478 ± 0.316
3.449GlnArg: 3.449 ± 0.449
2.463GlnSer: 2.463 ± 0.45
1.689GlnThr: 1.689 ± 0.277
2.745GlnVal: 2.745 ± 0.53
0.774GlnTrp: 0.774 ± 0.32
0.915GlnTyr: 0.915 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
11.12ArgAla: 11.12 ± 1.172
0.633ArgCys: 0.633 ± 0.213
4.293ArgAsp: 4.293 ± 0.634
5.349ArgGlu: 5.349 ± 0.794
3.238ArgPhe: 3.238 ± 0.549
4.505ArgGly: 4.505 ± 0.594
1.689ArgHis: 1.689 ± 0.385
4.505ArgIle: 4.505 ± 0.531
3.308ArgLys: 3.308 ± 0.5
5.138ArgLeu: 5.138 ± 0.714
1.337ArgMet: 1.337 ± 0.329
2.534ArgAsn: 2.534 ± 0.516
3.238ArgPro: 3.238 ± 0.667
2.534ArgGln: 2.534 ± 0.379
6.334ArgArg: 6.334 ± 0.781
3.59ArgSer: 3.59 ± 0.609
4.786ArgThr: 4.786 ± 0.433
6.053ArgVal: 6.053 ± 0.704
0.915ArgTrp: 0.915 ± 0.252
1.9ArgTyr: 1.9 ± 0.468
0.0ArgXaa: 0.0 ± 0.0
Ser
6.968SerAla: 6.968 ± 0.789
1.197SerCys: 1.197 ± 0.305
4.364SerAsp: 4.364 ± 0.639
3.308SerGlu: 3.308 ± 0.449
1.548SerPhe: 1.548 ± 0.338
5.842SerGly: 5.842 ± 0.685
0.774SerHis: 0.774 ± 0.18
2.604SerIle: 2.604 ± 0.331
2.815SerLys: 2.815 ± 0.519
4.716SerLeu: 4.716 ± 0.603
1.76SerMet: 1.76 ± 0.347
1.619SerAsn: 1.619 ± 0.308
3.097SerPro: 3.097 ± 0.505
1.971SerGln: 1.971 ± 0.281
4.223SerArg: 4.223 ± 0.498
4.575SerSer: 4.575 ± 0.714
2.675SerThr: 2.675 ± 0.452
4.153SerVal: 4.153 ± 0.635
1.056SerTrp: 1.056 ± 0.265
1.197SerTyr: 1.197 ± 0.476
0.0SerXaa: 0.0 ± 0.0
Thr
7.249ThrAla: 7.249 ± 0.696
0.493ThrCys: 0.493 ± 0.221
2.745ThrAsp: 2.745 ± 0.385
3.66ThrGlu: 3.66 ± 0.501
1.83ThrPhe: 1.83 ± 0.381
3.73ThrGly: 3.73 ± 0.587
0.774ThrHis: 0.774 ± 0.234
2.323ThrIle: 2.323 ± 0.389
2.252ThrLys: 2.252 ± 0.382
4.505ThrLeu: 4.505 ± 0.599
1.126ThrMet: 1.126 ± 0.218
1.619ThrAsn: 1.619 ± 0.366
3.238ThrPro: 3.238 ± 0.465
1.83ThrGln: 1.83 ± 0.321
2.745ThrArg: 2.745 ± 0.544
3.167ThrSer: 3.167 ± 0.553
3.167ThrThr: 3.167 ± 0.497
4.434ThrVal: 4.434 ± 0.611
1.056ThrTrp: 1.056 ± 0.332
1.267ThrTyr: 1.267 ± 0.326
0.0ThrXaa: 0.0 ± 0.0
Val
8.446ValAla: 8.446 ± 0.942
0.563ValCys: 0.563 ± 0.199
3.871ValAsp: 3.871 ± 0.588
3.73ValGlu: 3.73 ± 0.492
2.604ValPhe: 2.604 ± 0.396
5.56ValGly: 5.56 ± 0.806
1.478ValHis: 1.478 ± 0.325
3.167ValIle: 3.167 ± 0.485
3.097ValLys: 3.097 ± 0.516
4.856ValLeu: 4.856 ± 0.625
1.619ValMet: 1.619 ± 0.333
1.971ValAsn: 1.971 ± 0.364
2.815ValPro: 2.815 ± 0.459
2.745ValGln: 2.745 ± 0.494
5.49ValArg: 5.49 ± 0.775
4.716ValSer: 4.716 ± 0.695
3.941ValThr: 3.941 ± 0.479
3.871ValVal: 3.871 ± 0.606
1.197ValTrp: 1.197 ± 0.283
1.83ValTyr: 1.83 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
2.323TrpAla: 2.323 ± 0.49
0.141TrpCys: 0.141 ± 0.099
0.633TrpAsp: 0.633 ± 0.233
0.493TrpGlu: 0.493 ± 0.163
0.352TrpPhe: 0.352 ± 0.136
0.774TrpGly: 0.774 ± 0.192
0.211TrpHis: 0.211 ± 0.133
1.126TrpIle: 1.126 ± 0.304
0.915TrpLys: 0.915 ± 0.285
1.689TrpLeu: 1.689 ± 0.368
0.563TrpMet: 0.563 ± 0.219
0.563TrpAsn: 0.563 ± 0.178
0.493TrpPro: 0.493 ± 0.221
1.337TrpGln: 1.337 ± 0.333
1.971TrpArg: 1.971 ± 0.448
1.056TrpSer: 1.056 ± 0.277
0.704TrpThr: 0.704 ± 0.192
1.548TrpVal: 1.548 ± 0.326
0.493TrpTrp: 0.493 ± 0.185
0.493TrpTyr: 0.493 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.167TyrAla: 3.167 ± 0.459
0.422TyrCys: 0.422 ± 0.162
1.408TyrAsp: 1.408 ± 0.286
1.267TyrGlu: 1.267 ± 0.332
0.563TyrPhe: 0.563 ± 0.185
2.111TyrGly: 2.111 ± 0.392
0.493TyrHis: 0.493 ± 0.183
1.056TyrIle: 1.056 ± 0.295
1.056TyrLys: 1.056 ± 0.273
1.83TyrLeu: 1.83 ± 0.37
0.282TyrMet: 0.282 ± 0.118
0.704TyrAsn: 0.704 ± 0.197
1.126TyrPro: 1.126 ± 0.28
0.915TyrGln: 0.915 ± 0.246
1.689TyrArg: 1.689 ± 0.346
1.83TyrSer: 1.83 ± 0.381
1.478TyrThr: 1.478 ± 0.4
1.971TyrVal: 1.971 ± 0.336
0.493TyrTrp: 0.493 ± 0.186
0.422TyrTyr: 0.422 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (14209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski