Robiginitalea myxolifaciens
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6HER6|A0A1I6HER6_9FLAO Polyphosphate glucokinase OS=Robiginitalea myxolifaciens OX=400055 GN=SAMN04490243_2660 PE=4 SV=1
MM1 pKa = 6.99 TRR3 pKa = 11.84 NLVFLLAFLWATYY16 pKa = 10.57 SLQAQNSPDD25 pKa = 3.77 CRR27 pKa = 11.84 SAIPVCADD35 pKa = 3.2 APILNFADD43 pKa = 3.73 GGGDD47 pKa = 3.65 IDD49 pKa = 6.32 DD50 pKa = 5.3 FDD52 pKa = 6.72 PDD54 pKa = 3.83 VIRR57 pKa = 11.84 QSGCLEE63 pKa = 4.22 KK64 pKa = 11.13 GSVASANIEE73 pKa = 4.33 NNTSWYY79 pKa = 9.78 VFRR82 pKa = 11.84 AGTGGQVGFDD92 pKa = 3.42 IEE94 pKa = 4.45 ALSDD98 pKa = 3.27 TAEE101 pKa = 4.0 WDD103 pKa = 3.62 FALYY107 pKa = 10.5 GPDD110 pKa = 3.83 VDD112 pKa = 5.97 CGDD115 pKa = 3.91 ISNGTAQPIRR125 pKa = 11.84 CNYY128 pKa = 8.15 EE129 pKa = 3.76 VNDD132 pKa = 3.71 TRR134 pKa = 11.84 FTGVGVNPEE143 pKa = 4.02 NGQAGQPFVKK153 pKa = 10.48 GSQNTYY159 pKa = 10.82 DD160 pKa = 3.21 EE161 pKa = 5.16 WIDD164 pKa = 3.66 VQPGEE169 pKa = 4.7 IYY171 pKa = 10.83 YY172 pKa = 10.82 LLINNYY178 pKa = 7.43 NTNFDD183 pKa = 4.4 GDD185 pKa = 4.2 PEE187 pKa = 4.72 PYY189 pKa = 10.57 SLTFTGNSVDD199 pKa = 4.05 ADD201 pKa = 3.31 QDD203 pKa = 3.66 NALDD207 pKa = 3.68 CTLRR211 pKa = 11.84 DD212 pKa = 3.55 EE213 pKa = 5.22 FLGLDD218 pKa = 3.54 IVACEE223 pKa = 4.1 GDD225 pKa = 3.61 PDD227 pKa = 3.97 IVLSALNSPAGPDD240 pKa = 3.07 IANVTWSVDD249 pKa = 3.54 YY250 pKa = 11.19 EE251 pKa = 4.36 DD252 pKa = 5.73 DD253 pKa = 4.42 GVIDD257 pKa = 4.09 AQLADD262 pKa = 4.17 GPGEE266 pKa = 4.18 TEE268 pKa = 4.02 FTVVSPISGRR278 pKa = 11.84 YY279 pKa = 7.43 YY280 pKa = 11.07 VEE282 pKa = 3.8 ILTTLATTITDD293 pKa = 4.65 DD294 pKa = 5.89 ILITWYY300 pKa = 10.27 GVPVLDD306 pKa = 5.39 RR307 pKa = 11.84 VDD309 pKa = 3.81 ILDD312 pKa = 5.41 DD313 pKa = 5.69 LSDD316 pKa = 3.53 QNNIQVFVQGDD327 pKa = 3.6 GDD329 pKa = 4.16 YY330 pKa = 11.45 EE331 pKa = 3.93 FAINNGPFQDD341 pKa = 4.19 DD342 pKa = 4.25 SIFRR346 pKa = 11.84 DD347 pKa = 3.94 VPPGINTLIINDD359 pKa = 3.81 KK360 pKa = 9.92 NGCGTTEE367 pKa = 4.52 PIEE370 pKa = 4.07 FLVVGYY376 pKa = 9.34 PKK378 pKa = 10.75 FFTPNNDD385 pKa = 3.17 TFNDD389 pKa = 3.21 TWQVKK394 pKa = 10.18 GIEE397 pKa = 4.13 TLIDD401 pKa = 3.4 PVVFIFDD408 pKa = 3.99 RR409 pKa = 11.84 YY410 pKa = 10.64 GKK412 pKa = 9.9 LLKK415 pKa = 10.74 QIDD418 pKa = 4.21 EE419 pKa = 4.68 TSLGWDD425 pKa = 3.34 GSFNGRR431 pKa = 11.84 PMPASDD437 pKa = 2.97 YY438 pKa = 10.13 WFRR441 pKa = 11.84 LEE443 pKa = 3.92 YY444 pKa = 10.89 SRR446 pKa = 11.84 DD447 pKa = 3.15 EE448 pKa = 4.33 SGIVVANTIRR458 pKa = 11.84 AHH460 pKa = 5.41 FTLKK464 pKa = 10.46 RR465 pKa = 3.52
Molecular weight: 51.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 1.367
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A1I6FMU4|A0A1I6FMU4_9FLAO DUF4328 domain-containing protein OS=Robiginitalea myxolifaciens OX=400055 GN=SAMN04490243_0087 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.27 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.52 LSVSSEE47 pKa = 3.87 PRR49 pKa = 11.84 HH50 pKa = 5.89 KK51 pKa = 10.65 RR52 pKa = 3.21
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.789
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.544
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.296
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.018
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2844
0
2844
998853
31
4472
351.2
39.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.924 ± 0.044
0.728 ± 0.015
5.703 ± 0.051
6.956 ± 0.052
4.821 ± 0.033
7.533 ± 0.052
1.799 ± 0.023
6.443 ± 0.033
5.152 ± 0.058
10.182 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.024
4.554 ± 0.041
4.218 ± 0.034
3.801 ± 0.027
5.063 ± 0.044
6.231 ± 0.037
5.393 ± 0.06
6.278 ± 0.039
1.22 ± 0.018
3.724 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here