Acetivibrio saccincola
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9E8F9|A0A2K9E8F9_9FIRM Uncharacterized protein OS=Acetivibrio saccincola OX=1677857 GN=B9R14_04590 PE=4 SV=1
MM1 pKa = 6.99 MVCLFIFSAFSLLNITGVLAEE22 pKa = 4.34 EE23 pKa = 4.91 DD24 pKa = 4.0 VLSVLDD30 pKa = 3.95 FEE32 pKa = 6.3 DD33 pKa = 3.57 YY34 pKa = 10.57 FYY36 pKa = 11.61 YY37 pKa = 10.65 EE38 pKa = 4.61 DD39 pKa = 3.7 GNYY42 pKa = 10.23 VLTSDD47 pKa = 3.92 IVVDD51 pKa = 3.29 SSFRR55 pKa = 11.84 IDD57 pKa = 3.06 GHH59 pKa = 4.7 TVVLNGYY66 pKa = 9.93 SMTVEE71 pKa = 4.16 GDD73 pKa = 3.42 LCIDD77 pKa = 4.03 NGSLDD82 pKa = 4.23 LGSGSLTVNGDD93 pKa = 3.41 FYY95 pKa = 11.2 INGFDD100 pKa = 3.54 TSNPAVLDD108 pKa = 3.7 INNGNLIIEE117 pKa = 4.75 GNFDD121 pKa = 3.34 TYY123 pKa = 11.85 DD124 pKa = 3.44 NVILYY129 pKa = 7.3 MVQEE133 pKa = 3.91 NDD135 pKa = 3.24 YY136 pKa = 11.24 VLVHH140 pKa = 6.79 KK141 pKa = 10.54 DD142 pKa = 3.38 FYY144 pKa = 10.92 FNSFIPHH151 pKa = 6.58 GDD153 pKa = 3.35 YY154 pKa = 10.99 LINGTLEE161 pKa = 4.26 VKK163 pKa = 10.7 GDD165 pKa = 3.75 FYY167 pKa = 11.75 DD168 pKa = 4.57 FSKK171 pKa = 11.57 GNFLPSQNHH180 pKa = 4.62 RR181 pKa = 11.84 VVLSGEE187 pKa = 3.95 DD188 pKa = 3.39 VQYY191 pKa = 10.33 VTFEE195 pKa = 4.11 YY196 pKa = 10.54 PEE198 pKa = 4.4 LSSFNILEE206 pKa = 4.09 LTKK209 pKa = 10.19 PLEE212 pKa = 4.05 TGYY215 pKa = 10.73 VFSHH219 pKa = 5.26 TPVWNTLIEE228 pKa = 4.86 PGTSFKK234 pKa = 11.09 PGDD237 pKa = 3.74 LDD239 pKa = 4.35 GNGTVDD245 pKa = 3.47 STDD248 pKa = 2.89 ATILKK253 pKa = 9.72 RR254 pKa = 11.84 YY255 pKa = 8.17 IVSIIDD261 pKa = 4.29 EE262 pKa = 4.42 IPAGNDD268 pKa = 3.1 AADD271 pKa = 3.91 LNGDD275 pKa = 3.78 GDD277 pKa = 4.43 IDD279 pKa = 4.17 SLDD282 pKa = 3.53 YY283 pKa = 10.95 TLLNRR288 pKa = 11.84 FILGMITEE296 pKa = 4.78 FPVNRR301 pKa = 4.3
Molecular weight: 33.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.846
Patrickios 0.388
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A2K9E631|A0A2K9E631_9FIRM Arginine decarboxylase OS=Acetivibrio saccincola OX=1677857 GN=speA2 PE=3 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.8 QPKK8 pKa = 9.46 KK9 pKa = 8.28 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.77 RR21 pKa = 11.84 MKK23 pKa = 8.64 TSNGRR28 pKa = 11.84 KK29 pKa = 7.18 VLRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.26 GRR39 pKa = 11.84 KK40 pKa = 8.78 VLSAA44 pKa = 4.05
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3020
0
3020
967809
29
3652
320.5
36.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.703 ± 0.043
1.063 ± 0.015
5.81 ± 0.034
7.985 ± 0.042
4.385 ± 0.033
6.643 ± 0.048
1.469 ± 0.018
9.06 ± 0.039
8.574 ± 0.054
8.499 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.019
5.569 ± 0.04
3.286 ± 0.032
2.393 ± 0.024
4.047 ± 0.033
6.111 ± 0.033
4.946 ± 0.048
6.832 ± 0.039
0.877 ± 0.015
4.287 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here