Rice hoja blanca tenuivirus
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5CMS9|A0A2H5CMS9_9VIRU NS3 protein OS=Rice hoja blanca tenuivirus OX=12332 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 4.8 FLKK5 pKa = 10.2 TDD7 pKa = 3.35 VSVGPIEE14 pKa = 4.09 GLNYY18 pKa = 9.71 RR19 pKa = 11.84 RR20 pKa = 11.84 LYY22 pKa = 10.73 DD23 pKa = 3.42 ILPNKK28 pKa = 10.0 VSDD31 pKa = 4.57 NITLPDD37 pKa = 4.28 LKK39 pKa = 11.15 NPDD42 pKa = 3.55 KK43 pKa = 10.87 VTEE46 pKa = 4.16 EE47 pKa = 3.89 NKK49 pKa = 10.68 KK50 pKa = 10.84 LILCGFIYY58 pKa = 10.39 VAYY61 pKa = 9.2 HH62 pKa = 6.18 HH63 pKa = 7.17 PIEE66 pKa = 4.48 TDD68 pKa = 3.1 PDD70 pKa = 3.6 FTSVHH75 pKa = 5.02 KK76 pKa = 10.29 HH77 pKa = 4.52 MPGISEE83 pKa = 4.3 SFLEE87 pKa = 4.36 HH88 pKa = 7.3 LLGTDD93 pKa = 3.58 EE94 pKa = 4.74 SNNTIDD100 pKa = 4.6 LGKK103 pKa = 10.64 LFDD106 pKa = 4.05 ILQEE110 pKa = 4.2 RR111 pKa = 11.84 LGDD114 pKa = 4.18 WITMNFLKK122 pKa = 10.71 HH123 pKa = 5.63 NNRR126 pKa = 11.84 MSKK129 pKa = 10.49 DD130 pKa = 3.53 QIKK133 pKa = 8.01 TLCEE137 pKa = 4.15 TIVDD141 pKa = 4.29 LAKK144 pKa = 10.91 AEE146 pKa = 4.39 GGDD149 pKa = 3.66 TEE151 pKa = 4.71 IYY153 pKa = 8.75 EE154 pKa = 4.55 AVWKK158 pKa = 10.6 KK159 pKa = 9.58 MPAYY163 pKa = 10.47 YY164 pKa = 10.31 SILLQQILHH173 pKa = 6.51 KK174 pKa = 10.88
Molecular weight: 20.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.17
IPC2_protein 5.181
IPC_protein 5.13
Toseland 5.232
ProMoST 5.347
Dawson 5.219
Bjellqvist 5.308
Wikipedia 5.143
Rodwell 5.143
Grimsley 5.219
Solomon 5.219
Lehninger 5.181
Nozaki 5.385
DTASelect 5.563
Thurlkill 5.296
EMBOSS 5.27
Sillero 5.448
Patrickios 4.012
IPC_peptide 5.232
IPC2_peptide 5.448
IPC2.peptide.svr19 5.435
Protein with the highest isoelectric point:
>tr|Q08349|Q08349_9VIRU Movement protein NS4 OS=Rice hoja blanca tenuivirus OX=12332 GN=NS4vc PE=4 SV=1
MM1 pKa = 7.49 SISRR5 pKa = 11.84 YY6 pKa = 9.93 NPFKK10 pKa = 10.8 KK11 pKa = 10.42 SVILTDD17 pKa = 4.08 DD18 pKa = 3.46 LSEE21 pKa = 4.01 RR22 pKa = 11.84 AAEE25 pKa = 4.16 KK26 pKa = 10.66 FEE28 pKa = 4.35 KK29 pKa = 10.28 KK30 pKa = 9.84 NKK32 pKa = 9.82 RR33 pKa = 11.84 KK34 pKa = 9.3 LALKK38 pKa = 10.0 NRR40 pKa = 11.84 PLTKK44 pKa = 10.6 GRR46 pKa = 11.84 MTIDD50 pKa = 2.83 AAATVLGLEE59 pKa = 4.45 PFSFADD65 pKa = 3.58 VRR67 pKa = 11.84 ANSYY71 pKa = 11.86 DD72 pKa = 3.51 MFVAKK77 pKa = 9.87 QDD79 pKa = 3.61 YY80 pKa = 9.58 SVCANRR86 pKa = 11.84 RR87 pKa = 11.84 THH89 pKa = 5.79 FTIDD93 pKa = 3.17 SSPLFFRR100 pKa = 11.84 KK101 pKa = 9.28 PLQTFPFFRR110 pKa = 11.84 IATFAVIWLGIKK122 pKa = 9.91 GRR124 pKa = 11.84 ANGTVTFRR132 pKa = 11.84 IIDD135 pKa = 3.47 RR136 pKa = 11.84 SYY138 pKa = 10.63 TDD140 pKa = 3.49 PEE142 pKa = 4.02 RR143 pKa = 11.84 QVEE146 pKa = 4.22 VEE148 pKa = 3.5 ICYY151 pKa = 10.5 PMAKK155 pKa = 9.0 TFAVLGSLPNFMSYY169 pKa = 10.63 EE170 pKa = 4.35 DD171 pKa = 3.95 ADD173 pKa = 3.73 KK174 pKa = 10.08 MQVEE178 pKa = 4.57 IVIKK182 pKa = 10.44 DD183 pKa = 3.71 DD184 pKa = 3.76 SVQNCIISRR193 pKa = 11.84 SLWFWGIEE201 pKa = 3.93 RR202 pKa = 11.84 TDD204 pKa = 3.94 FPVPMEE210 pKa = 4.12 SQKK213 pKa = 9.62 TVMFEE218 pKa = 4.16 FEE220 pKa = 4.63 PLPDD224 pKa = 2.95 RR225 pKa = 11.84 TVNHH229 pKa = 6.73 LSKK232 pKa = 10.77 FKK234 pKa = 11.17 NFTTDD239 pKa = 2.74 VVQRR243 pKa = 11.84 AVTTAFTTRR252 pKa = 11.84 EE253 pKa = 4.02 ALEE256 pKa = 4.57 DD257 pKa = 3.46 KK258 pKa = 10.64 PGIEE262 pKa = 4.35 FGVVKK267 pKa = 10.62 QPGVPLVQKK276 pKa = 10.0 KK277 pKa = 9.21 RR278 pKa = 11.84 VMIEE282 pKa = 3.52 AA283 pKa = 4.29
Molecular weight: 32.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.812
IPC2_protein 8.595
IPC_protein 8.521
Toseland 9.399
ProMoST 9.077
Dawson 9.619
Bjellqvist 9.326
Wikipedia 9.75
Rodwell 9.999
Grimsley 9.663
Solomon 9.677
Lehninger 9.663
Nozaki 9.531
DTASelect 9.282
Thurlkill 9.487
EMBOSS 9.823
Sillero 9.589
Patrickios 5.728
IPC_peptide 9.677
IPC2_peptide 7.863
IPC2.peptide.svr19 7.837
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4931
174
2918
704.4
80.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.847 ± 0.474
1.987 ± 0.822
6.469 ± 0.167
6.388 ± 0.6
4.502 ± 0.372
4.279 ± 0.454
2.373 ± 0.306
6.814 ± 0.588
7.869 ± 0.387
9.592 ± 0.492
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.022 ± 0.182
4.786 ± 0.398
3.955 ± 0.533
2.88 ± 0.384
4.421 ± 0.568
7.747 ± 0.586
6.246 ± 0.624
6.591 ± 0.557
1.034 ± 0.129
4.198 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here