Ying Kou virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses; Negevirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1Z2Y5|A0A3G1Z2Y5_9VIRU Uncharacterized protein OS=Ying Kou virus OX=2479372 GN=ORF3 PE=4 SV=1
MM1 pKa = 8.26LLMILIISVVPFVRR15 pKa = 11.84SAVFVKK21 pKa = 10.6DD22 pKa = 3.19VTAQLQNTRR31 pKa = 11.84EE32 pKa = 3.84RR33 pKa = 11.84LILYY37 pKa = 9.57SKK39 pKa = 10.32IKK41 pKa = 9.64PRR43 pKa = 11.84SHH45 pKa = 6.8FVGEE49 pKa = 4.22YY50 pKa = 10.71DD51 pKa = 3.39VTPYY55 pKa = 10.89SFEE58 pKa = 4.16KK59 pKa = 10.42EE60 pKa = 4.03CMSIYY65 pKa = 9.38RR66 pKa = 11.84TNDD69 pKa = 2.57WLFANCEE76 pKa = 4.16LPAHH80 pKa = 6.94CSTPLVSKK88 pKa = 10.65INKK91 pKa = 8.29NWLGQEE97 pKa = 3.54IVLCHH102 pKa = 5.83GVHH105 pKa = 6.28PVVDD109 pKa = 4.18TPRR112 pKa = 11.84LEE114 pKa = 4.11FFTVDD119 pKa = 4.7FIPQTFSFGRR129 pKa = 11.84PISFEE134 pKa = 3.83FGKK137 pKa = 10.92VSNFTNFQPVSFFEE151 pKa = 3.77WFYY154 pKa = 11.22VVRR157 pKa = 11.84GPDD160 pKa = 3.19GYY162 pKa = 11.53GIVPKK167 pKa = 10.39FCSEE171 pKa = 3.92TFRR174 pKa = 11.84NSTALPPNVEE184 pKa = 3.82IHH186 pKa = 6.78SDD188 pKa = 3.68GDD190 pKa = 4.09SLCLRR195 pKa = 11.84RR196 pKa = 11.84VLFDD200 pKa = 4.99KK201 pKa = 10.89NDD203 pKa = 3.59CRR205 pKa = 11.84PTFYY209 pKa = 11.1EE210 pKa = 4.43SFTGLLFKK218 pKa = 11.43YY219 pKa = 9.22NFPVQFDD226 pKa = 4.08GAPFSQGAYY235 pKa = 10.03ASTNNKK241 pKa = 9.47VIGADD246 pKa = 3.28HH247 pKa = 6.16YY248 pKa = 7.73TTSNSGKK255 pKa = 10.07LPTHH259 pKa = 7.15IIDD262 pKa = 3.55KK263 pKa = 10.1VGRR266 pKa = 11.84SYY268 pKa = 10.78MVTYY272 pKa = 10.66SSYY275 pKa = 11.17EE276 pKa = 3.98DD277 pKa = 4.13PEE279 pKa = 4.61PIYY282 pKa = 10.12IVKK285 pKa = 8.72ATQVVPFEE293 pKa = 5.14DD294 pKa = 4.4SEE296 pKa = 4.53CFPIVSKK303 pKa = 10.42YY304 pKa = 8.96QSPIEE309 pKa = 4.71HH310 pKa = 6.84ILKK313 pKa = 10.24KK314 pKa = 10.13ISNFFRR320 pKa = 11.84DD321 pKa = 3.76EE322 pKa = 3.88IEE324 pKa = 4.06YY325 pKa = 10.79LLEE328 pKa = 4.21FFLVVAEE335 pKa = 5.09KK336 pKa = 10.33IASILVAIIGEE347 pKa = 4.4LLSLITTLIPYY358 pKa = 10.37GEE360 pKa = 4.52LFYY363 pKa = 10.93TSFFLACVTYY373 pKa = 10.85VFTRR377 pKa = 11.84DD378 pKa = 3.08VLLSVFPTVSIYY390 pKa = 10.92LLRR393 pKa = 11.84IYY395 pKa = 10.63LKK397 pKa = 9.86TVINN401 pKa = 4.03

Molecular weight: 45.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1Z324|A0A3G1Z324_9VIRU Non-structural protein 3 OS=Ying Kou virus OX=2479372 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.24SALRR5 pKa = 11.84AKK7 pKa = 10.43KK8 pKa = 9.76NSTVARR14 pKa = 11.84LAPVRR19 pKa = 11.84LAPPPARR26 pKa = 11.84VVVAKK31 pKa = 9.98KK32 pKa = 10.3AKK34 pKa = 9.34PSSPRR39 pKa = 11.84DD40 pKa = 3.25SGFDD44 pKa = 3.63LNKK47 pKa = 9.79VFSDD51 pKa = 3.65VSSTFVAGLNQPLVLLTLVLVAALVFTHH79 pKa = 6.54VNDD82 pKa = 4.81FSSGVVGKK90 pKa = 9.07WVSEE94 pKa = 4.02KK95 pKa = 10.93SATNSLALWVHH106 pKa = 5.12QNQVKK111 pKa = 9.98FLGLAIFAPAVLDD124 pKa = 3.66SPSRR128 pKa = 11.84LRR130 pKa = 11.84VPLALASVFWVMLVPQASIYY150 pKa = 10.25EE151 pKa = 4.09YY152 pKa = 10.55AIQAVALHH160 pKa = 6.32TYY162 pKa = 10.11FRR164 pKa = 11.84VKK166 pKa = 10.27LQNSRR171 pKa = 11.84ILIILSVAALYY182 pKa = 10.05FFGHH186 pKa = 6.49MSLAVPEE193 pKa = 4.3IKK195 pKa = 9.3KK196 pKa = 7.99TANSTLSSGG205 pKa = 3.57

Molecular weight: 22.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2963

205

2357

987.7

112.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.062 ± 1.826

2.092 ± 0.503

5.737 ± 1.099

5.737 ± 1.049

6.581 ± 0.997

4.387 ± 0.258

2.497 ± 0.26

5.029 ± 1.027

5.299 ± 0.228

9.112 ± 0.972

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.517

5.164 ± 0.606

4.522 ± 0.803

2.734 ± 0.184

5.872 ± 0.886

9.146 ± 0.548

5.501 ± 0.478

8.91 ± 1.117

0.54 ± 0.257

3.915 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski