Prochlorococcus marinus (strain NATL2A)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2142 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q46KY3|Q46KY3_PROMT ATP-dependent zinc metalloprotease FtsH OS=Prochlorococcus marinus (strain NATL2A) OX=59920 GN=ftsH PE=3 SV=1
MM1 pKa = 7.55 VEE3 pKa = 3.79 QNALKK8 pKa = 10.98 VKK10 pKa = 10.33 DD11 pKa = 4.51 CYY13 pKa = 11.11 SHH15 pKa = 6.57 QNLSIFTLGEE25 pKa = 3.95 VIEE28 pKa = 4.68 ISSNQYY34 pKa = 10.55 GLSLFSGMISLLALGVYY51 pKa = 9.19 TLLTVDD57 pKa = 5.1 AGNDD61 pKa = 3.75 DD62 pKa = 4.89 DD63 pKa = 6.89 DD64 pKa = 6.18 SDD66 pKa = 4.2 SGSGGLMQPVNN77 pKa = 3.75
Molecular weight: 8.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.77
IPC_protein 3.668
Toseland 3.465
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.795
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|Q46JP3|Q46JP3_PROMT Flavodoxin OS=Prochlorococcus marinus (strain NATL2A) OX=59920 GN=PMN2A_0794 PE=3 SV=1
MM1 pKa = 7.41 TKK3 pKa = 9.05 RR4 pKa = 11.84 TFGGTSRR11 pKa = 11.84 KK12 pKa = 9.13 RR13 pKa = 11.84 KK14 pKa = 8.17 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 SHH26 pKa = 6.1 TGRR29 pKa = 11.84 RR30 pKa = 11.84 VVRR33 pKa = 11.84 TRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.18 RR38 pKa = 11.84 GRR40 pKa = 11.84 SRR42 pKa = 11.84 LTVV45 pKa = 3.04
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2142
0
2142
534346
27
1821
249.5
27.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.466 ± 0.082
1.135 ± 0.018
5.245 ± 0.051
6.544 ± 0.052
4.317 ± 0.046
6.81 ± 0.059
1.68 ± 0.024
7.998 ± 0.062
7.188 ± 0.065
11.131 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.022
5.326 ± 0.055
4.082 ± 0.032
3.451 ± 0.032
4.523 ± 0.047
7.834 ± 0.046
4.717 ± 0.042
5.572 ± 0.055
1.428 ± 0.028
2.55 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here