Amino acid dipepetide frequency for Microbacterium sp. TNHR37B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.296AlaAla: 21.296 ± 0.223
0.678AlaCys: 0.678 ± 0.026
8.468AlaAsp: 8.468 ± 0.103
8.006AlaGlu: 8.006 ± 0.1
3.998AlaPhe: 3.998 ± 0.063
12.364AlaGly: 12.364 ± 0.108
2.608AlaHis: 2.608 ± 0.061
5.674AlaIle: 5.674 ± 0.091
2.251AlaLys: 2.251 ± 0.06
14.447AlaLeu: 14.447 ± 0.151
2.486AlaMet: 2.486 ± 0.047
1.981AlaAsn: 1.981 ± 0.047
7.054AlaPro: 7.054 ± 0.116
3.792AlaGln: 3.792 ± 0.056
10.183AlaArg: 10.183 ± 0.142
7.39AlaSer: 7.39 ± 0.091
7.99AlaThr: 7.99 ± 0.109
12.23AlaVal: 12.23 ± 0.134
2.081AlaTrp: 2.081 ± 0.049
2.4AlaTyr: 2.4 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.73CysAla: 0.73 ± 0.027
0.058CysCys: 0.058 ± 0.007
0.328CysAsp: 0.328 ± 0.017
0.256CysGlu: 0.256 ± 0.017
0.157CysPhe: 0.157 ± 0.012
0.558CysGly: 0.558 ± 0.025
0.109CysHis: 0.109 ± 0.01
0.173CysIle: 0.173 ± 0.011
0.049CysLys: 0.049 ± 0.007
0.411CysLeu: 0.411 ± 0.018
0.062CysMet: 0.062 ± 0.007
0.092CysAsn: 0.092 ± 0.01
0.278CysPro: 0.278 ± 0.018
0.111CysGln: 0.111 ± 0.011
0.334CysArg: 0.334 ± 0.018
0.288CysSer: 0.288 ± 0.017
0.311CysThr: 0.311 ± 0.02
0.378CysVal: 0.378 ± 0.017
0.054CysTrp: 0.054 ± 0.007
0.085CysTyr: 0.085 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
9.304AspAla: 9.304 ± 0.112
0.233AspCys: 0.233 ± 0.015
4.618AspAsp: 4.618 ± 0.064
4.204AspGlu: 4.204 ± 0.071
1.646AspPhe: 1.646 ± 0.04
6.424AspGly: 6.424 ± 0.099
1.246AspHis: 1.246 ± 0.037
2.299AspIle: 2.299 ± 0.046
0.94AspLys: 0.94 ± 0.034
6.435AspLeu: 6.435 ± 0.092
0.787AspMet: 0.787 ± 0.028
0.951AspAsn: 0.951 ± 0.03
4.455AspPro: 4.455 ± 0.078
1.45AspGln: 1.45 ± 0.046
4.684AspArg: 4.684 ± 0.076
2.513AspSer: 2.513 ± 0.047
2.996AspThr: 2.996 ± 0.059
5.624AspVal: 5.624 ± 0.085
0.899AspTrp: 0.899 ± 0.034
1.303AspTyr: 1.303 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
7.0GluAla: 7.0 ± 0.105
0.233GluCys: 0.233 ± 0.014
2.652GluAsp: 2.652 ± 0.053
3.115GluGlu: 3.115 ± 0.06
1.627GluPhe: 1.627 ± 0.035
4.18GluGly: 4.18 ± 0.066
1.297GluHis: 1.297 ± 0.036
2.717GluIle: 2.717 ± 0.055
1.315GluLys: 1.315 ± 0.048
6.065GluLeu: 6.065 ± 0.095
0.851GluMet: 0.851 ± 0.028
1.15GluAsn: 1.15 ± 0.038
2.955GluPro: 2.955 ± 0.062
1.864GluGln: 1.864 ± 0.046
5.196GluArg: 5.196 ± 0.094
2.835GluSer: 2.835 ± 0.053
3.072GluThr: 3.072 ± 0.064
4.777GluVal: 4.777 ± 0.066
0.905GluTrp: 0.905 ± 0.029
1.141GluTyr: 1.141 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.272PheAla: 4.272 ± 0.072
0.14PheCys: 0.14 ± 0.012
2.357PheAsp: 2.357 ± 0.05
1.584PheGlu: 1.584 ± 0.041
1.098PhePhe: 1.098 ± 0.037
3.251PheGly: 3.251 ± 0.054
0.582PheHis: 0.582 ± 0.025
1.061PheIle: 1.061 ± 0.033
0.352PheLys: 0.352 ± 0.02
2.905PheLeu: 2.905 ± 0.063
0.405PheMet: 0.405 ± 0.021
0.547PheAsn: 0.547 ± 0.023
1.445PhePro: 1.445 ± 0.037
0.749PheGln: 0.749 ± 0.027
1.919PheArg: 1.919 ± 0.039
1.758PheSer: 1.758 ± 0.041
2.166PheThr: 2.166 ± 0.057
2.896PheVal: 2.896 ± 0.064
0.53PheTrp: 0.53 ± 0.022
0.615PheTyr: 0.615 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
11.181GlyAla: 11.181 ± 0.121
0.534GlyCys: 0.534 ± 0.021
5.31GlyAsp: 5.31 ± 0.081
5.034GlyGlu: 5.034 ± 0.069
3.119GlyPhe: 3.119 ± 0.057
7.696GlyGly: 7.696 ± 0.116
1.811GlyHis: 1.811 ± 0.047
4.461GlyIle: 4.461 ± 0.071
2.035GlyLys: 2.035 ± 0.056
8.465GlyLeu: 8.465 ± 0.097
1.859GlyMet: 1.859 ± 0.038
1.671GlyAsn: 1.671 ± 0.048
3.782GlyPro: 3.782 ± 0.063
2.41GlyGln: 2.41 ± 0.055
6.431GlyArg: 6.431 ± 0.079
5.384GlySer: 5.384 ± 0.093
5.797GlyThr: 5.797 ± 0.108
8.244GlyVal: 8.244 ± 0.089
1.742GlyTrp: 1.742 ± 0.048
2.272GlyTyr: 2.272 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.577HisAla: 2.577 ± 0.056
0.111HisCys: 0.111 ± 0.011
1.394HisAsp: 1.394 ± 0.039
1.106HisGlu: 1.106 ± 0.033
0.526HisPhe: 0.526 ± 0.025
1.953HisGly: 1.953 ± 0.045
0.568HisHis: 0.568 ± 0.028
0.676HisIle: 0.676 ± 0.024
0.249HisLys: 0.249 ± 0.018
2.047HisLeu: 2.047 ± 0.044
0.285HisMet: 0.285 ± 0.017
0.32HisAsn: 0.32 ± 0.02
1.544HisPro: 1.544 ± 0.042
0.48HisGln: 0.48 ± 0.021
1.697HisArg: 1.697 ± 0.047
0.87HisSer: 0.87 ± 0.029
1.016HisThr: 1.016 ± 0.032
1.76HisVal: 1.76 ± 0.041
0.292HisTrp: 0.292 ± 0.016
0.371HisTyr: 0.371 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.741IleAla: 6.741 ± 0.101
0.211IleCys: 0.211 ± 0.015
3.516IleAsp: 3.516 ± 0.055
2.638IleGlu: 2.638 ± 0.056
1.057IlePhe: 1.057 ± 0.037
4.333IleGly: 4.333 ± 0.067
0.696IleHis: 0.696 ± 0.024
1.542IleIle: 1.542 ± 0.048
0.69IleLys: 0.69 ± 0.026
3.531IleLeu: 3.531 ± 0.073
0.536IleMet: 0.536 ± 0.022
0.789IleAsn: 0.789 ± 0.027
2.386IlePro: 2.386 ± 0.046
0.914IleGln: 0.914 ± 0.032
2.593IleArg: 2.593 ± 0.052
2.242IleSer: 2.242 ± 0.049
2.705IleThr: 2.705 ± 0.066
4.664IleVal: 4.664 ± 0.085
0.53IleTrp: 0.53 ± 0.024
0.667IleTyr: 0.667 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
2.191LysAla: 2.191 ± 0.064
0.066LysCys: 0.066 ± 0.008
1.025LysAsp: 1.025 ± 0.036
0.87LysGlu: 0.87 ± 0.033
0.391LysPhe: 0.391 ± 0.021
1.452LysGly: 1.452 ± 0.048
0.322LysHis: 0.322 ± 0.019
0.858LysIle: 0.858 ± 0.036
0.756LysLys: 0.756 ± 0.038
1.592LysLeu: 1.592 ± 0.047
0.319LysMet: 0.319 ± 0.017
0.462LysAsn: 0.462 ± 0.023
1.032LysPro: 1.032 ± 0.032
0.513LysGln: 0.513 ± 0.024
1.233LysArg: 1.233 ± 0.038
1.012LysSer: 1.012 ± 0.035
1.311LysThr: 1.311 ± 0.04
1.551LysVal: 1.551 ± 0.049
0.228LysTrp: 0.228 ± 0.014
0.364LysTyr: 0.364 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
14.545LeuAla: 14.545 ± 0.143
0.476LeuCys: 0.476 ± 0.023
6.662LeuAsp: 6.662 ± 0.084
4.643LeuGlu: 4.643 ± 0.08
2.919LeuPhe: 2.919 ± 0.069
8.576LeuGly: 8.576 ± 0.104
1.942LeuHis: 1.942 ± 0.046
4.207LeuIle: 4.207 ± 0.087
1.435LeuLys: 1.435 ± 0.045
10.278LeuLeu: 10.278 ± 0.144
1.525LeuMet: 1.525 ± 0.038
1.653LeuAsn: 1.653 ± 0.038
5.757LeuPro: 5.757 ± 0.078
2.52LeuGln: 2.52 ± 0.053
8.006LeuArg: 8.006 ± 0.117
6.03LeuSer: 6.03 ± 0.076
6.683LeuThr: 6.683 ± 0.08
9.247LeuVal: 9.247 ± 0.135
1.426LeuTrp: 1.426 ± 0.04
1.673LeuTyr: 1.673 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
1.915MetAla: 1.915 ± 0.043
0.095MetCys: 0.095 ± 0.008
0.722MetAsp: 0.722 ± 0.025
0.57MetGlu: 0.57 ± 0.023
0.47MetPhe: 0.47 ± 0.022
1.259MetGly: 1.259 ± 0.037
0.283MetHis: 0.283 ± 0.014
0.828MetIle: 0.828 ± 0.03
0.385MetLys: 0.385 ± 0.018
1.68MetLeu: 1.68 ± 0.036
0.287MetMet: 0.287 ± 0.016
0.447MetAsn: 0.447 ± 0.019
1.029MetPro: 1.029 ± 0.029
0.457MetGln: 0.457 ± 0.022
1.327MetArg: 1.327 ± 0.036
1.404MetSer: 1.404 ± 0.036
1.719MetThr: 1.719 ± 0.042
1.284MetVal: 1.284 ± 0.037
0.22MetTrp: 0.22 ± 0.013
0.261MetTyr: 0.261 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.297AsnAla: 2.297 ± 0.047
0.096AsnCys: 0.096 ± 0.009
1.139AsnAsp: 1.139 ± 0.035
0.878AsnGlu: 0.878 ± 0.029
0.577AsnPhe: 0.577 ± 0.025
1.749AsnGly: 1.749 ± 0.059
0.321AsnHis: 0.321 ± 0.016
0.699AsnIle: 0.699 ± 0.027
0.355AsnLys: 0.355 ± 0.023
1.741AsnLeu: 1.741 ± 0.039
0.279AsnMet: 0.279 ± 0.016
0.441AsnAsn: 0.441 ± 0.022
1.399AsnPro: 1.399 ± 0.042
0.489AsnGln: 0.489 ± 0.025
1.102AsnArg: 1.102 ± 0.034
0.857AsnSer: 0.857 ± 0.031
1.099AsnThr: 1.099 ± 0.055
1.625AsnVal: 1.625 ± 0.044
0.251AsnTrp: 0.251 ± 0.017
0.431AsnTyr: 0.431 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
7.629ProAla: 7.629 ± 0.102
0.191ProCys: 0.191 ± 0.013
4.045ProAsp: 4.045 ± 0.07
3.716ProGlu: 3.716 ± 0.069
1.723ProPhe: 1.723 ± 0.04
5.223ProGly: 5.223 ± 0.091
1.237ProHis: 1.237 ± 0.038
1.979ProIle: 1.979 ± 0.041
0.867ProLys: 0.867 ± 0.031
5.194ProLeu: 5.194 ± 0.074
0.819ProMet: 0.819 ± 0.028
0.875ProAsn: 0.875 ± 0.029
2.625ProPro: 2.625 ± 0.065
1.546ProGln: 1.546 ± 0.036
3.8ProArg: 3.8 ± 0.071
3.485ProSer: 3.485 ± 0.064
3.573ProThr: 3.573 ± 0.073
5.021ProVal: 5.021 ± 0.069
0.955ProTrp: 0.955 ± 0.027
1.009ProTyr: 1.009 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.366GlnAla: 3.366 ± 0.063
0.1GlnCys: 0.1 ± 0.01
1.174GlnAsp: 1.174 ± 0.034
1.264GlnGlu: 1.264 ± 0.034
0.81GlnPhe: 0.81 ± 0.029
1.902GlnGly: 1.902 ± 0.046
0.572GlnHis: 0.572 ± 0.024
1.317GlnIle: 1.317 ± 0.036
0.625GlnLys: 0.625 ± 0.027
2.827GlnLeu: 2.827 ± 0.05
0.455GlnMet: 0.455 ± 0.021
0.582GlnAsn: 0.582 ± 0.026
1.426GlnPro: 1.426 ± 0.037
0.992GlnGln: 0.992 ± 0.029
2.503GlnArg: 2.503 ± 0.051
1.438GlnSer: 1.438 ± 0.042
1.584GlnThr: 1.584 ± 0.036
2.186GlnVal: 2.186 ± 0.048
0.546GlnTrp: 0.546 ± 0.022
0.59GlnTyr: 0.59 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
9.762ArgAla: 9.762 ± 0.13
0.324ArgCys: 0.324 ± 0.019
4.663ArgAsp: 4.663 ± 0.08
4.635ArgGlu: 4.635 ± 0.075
2.443ArgPhe: 2.443 ± 0.052
5.971ArgGly: 5.971 ± 0.089
1.723ArgHis: 1.723 ± 0.041
3.584ArgIle: 3.584 ± 0.056
1.176ArgLys: 1.176 ± 0.038
7.624ArgLeu: 7.624 ± 0.112
1.665ArgMet: 1.665 ± 0.037
1.253ArgAsn: 1.253 ± 0.032
3.957ArgPro: 3.957 ± 0.078
1.996ArgGln: 1.996 ± 0.044
7.296ArgArg: 7.296 ± 0.117
4.25ArgSer: 4.25 ± 0.071
4.436ArgThr: 4.436 ± 0.068
6.601ArgVal: 6.601 ± 0.086
1.35ArgTrp: 1.35 ± 0.041
1.612ArgTyr: 1.612 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
7.566SerAla: 7.566 ± 0.103
0.271SerCys: 0.271 ± 0.019
3.356SerAsp: 3.356 ± 0.06
2.718SerGlu: 2.718 ± 0.049
1.896SerPhe: 1.896 ± 0.044
5.894SerGly: 5.894 ± 0.096
1.038SerHis: 1.038 ± 0.028
2.286SerIle: 2.286 ± 0.052
0.906SerLys: 0.906 ± 0.033
5.481SerLeu: 5.481 ± 0.074
1.114SerMet: 1.114 ± 0.027
0.922SerAsn: 0.922 ± 0.03
3.459SerPro: 3.459 ± 0.071
1.304SerGln: 1.304 ± 0.032
4.017SerArg: 4.017 ± 0.062
3.586SerSer: 3.586 ± 0.077
3.63SerThr: 3.63 ± 0.067
4.839SerVal: 4.839 ± 0.087
0.993SerTrp: 0.993 ± 0.031
1.195SerTyr: 1.195 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
8.332ThrAla: 8.332 ± 0.122
0.295ThrCys: 0.295 ± 0.022
3.641ThrAsp: 3.641 ± 0.058
2.972ThrGlu: 2.972 ± 0.062
1.959ThrPhe: 1.959 ± 0.051
6.097ThrGly: 6.097 ± 0.112
1.17ThrHis: 1.17 ± 0.034
2.909ThrIle: 2.909 ± 0.06
1.069ThrLys: 1.069 ± 0.038
6.233ThrLeu: 6.233 ± 0.089
0.912ThrMet: 0.912 ± 0.029
1.121ThrAsn: 1.121 ± 0.043
4.226ThrPro: 4.226 ± 0.09
1.47ThrGln: 1.47 ± 0.036
4.16ThrArg: 4.16 ± 0.075
3.696ThrSer: 3.696 ± 0.078
4.152ThrThr: 4.152 ± 0.082
6.273ThrVal: 6.273 ± 0.127
0.983ThrTrp: 0.983 ± 0.029
1.356ThrTyr: 1.356 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
12.457ValAla: 12.457 ± 0.133
0.489ValCys: 0.489 ± 0.022
5.835ValAsp: 5.835 ± 0.074
4.868ValGlu: 4.868 ± 0.073
2.96ValPhe: 2.96 ± 0.061
7.261ValGly: 7.261 ± 0.097
1.681ValHis: 1.681 ± 0.041
4.311ValIle: 4.311 ± 0.061
1.471ValLys: 1.471 ± 0.045
9.39ValLeu: 9.39 ± 0.124
1.399ValMet: 1.399 ± 0.044
1.634ValAsn: 1.634 ± 0.039
4.88ValPro: 4.88 ± 0.069
2.038ValGln: 2.038 ± 0.048
6.668ValArg: 6.668 ± 0.087
5.383ValSer: 5.383 ± 0.081
6.408ValThr: 6.408 ± 0.145
9.489ValVal: 9.489 ± 0.122
1.337ValTrp: 1.337 ± 0.042
1.607ValTyr: 1.607 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.711TrpAla: 1.711 ± 0.039
0.097TrpCys: 0.097 ± 0.01
0.821TrpAsp: 0.821 ± 0.029
0.773TrpGlu: 0.773 ± 0.033
0.576TrpPhe: 0.576 ± 0.025
1.244TrpGly: 1.244 ± 0.035
0.339TrpHis: 0.339 ± 0.02
0.732TrpIle: 0.732 ± 0.024
0.295TrpLys: 0.295 ± 0.017
1.728TrpLeu: 1.728 ± 0.046
0.361TrpMet: 0.361 ± 0.018
0.467TrpAsn: 0.467 ± 0.021
0.774TrpPro: 0.774 ± 0.027
0.605TrpGln: 0.605 ± 0.027
1.465TrpArg: 1.465 ± 0.041
1.015TrpSer: 1.015 ± 0.033
1.096TrpThr: 1.096 ± 0.039
1.238TrpVal: 1.238 ± 0.039
0.428TrpTrp: 0.428 ± 0.02
0.313TrpTyr: 0.313 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.505TyrAla: 2.505 ± 0.051
0.122TyrCys: 0.122 ± 0.011
1.285TyrAsp: 1.285 ± 0.034
1.098TyrGlu: 1.098 ± 0.031
0.677TyrPhe: 0.677 ± 0.024
1.853TyrGly: 1.853 ± 0.041
0.297TyrHis: 0.297 ± 0.016
0.671TyrIle: 0.671 ± 0.026
0.33TyrLys: 0.33 ± 0.021
2.129TyrLeu: 2.129 ± 0.05
0.233TyrMet: 0.233 ± 0.018
0.464TyrAsn: 0.464 ± 0.023
1.05TyrPro: 1.05 ± 0.029
0.559TyrGln: 0.559 ± 0.023
1.707TyrArg: 1.707 ± 0.046
1.039TyrSer: 1.039 ± 0.036
1.281TyrThr: 1.281 ± 0.045
1.638TyrVal: 1.638 ± 0.047
0.329TyrTrp: 0.329 ± 0.02
0.433TyrTyr: 0.433 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3221 proteins (1083291 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski