Amino acid dipepetide frequency for Noviherbaspirillum denitrificans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.16AlaAla: 15.16 ± 0.161	1.273AlaCys: 1.273 ± 0.03	5.891AlaAsp: 5.891 ± 0.057	6.733AlaGlu: 6.733 ± 0.103	4.26AlaPhe: 4.26 ± 0.05	9.753AlaGly: 9.753 ± 0.104	2.362AlaHis: 2.362 ± 0.041	5.952AlaIle: 5.952 ± 0.065	4.417AlaLys: 4.417 ± 0.059	12.594AlaLeu: 12.594 ± 0.101	3.434AlaMet: 3.434 ± 0.052	3.145AlaAsn: 3.145 ± 0.053	4.944AlaPro: 4.944 ± 0.069	4.536AlaGln: 4.536 ± 0.064	7.515AlaArg: 7.515 ± 0.079	6.554AlaSer: 6.554 ± 0.076	5.5AlaThr: 5.5 ± 0.06	8.463AlaVal: 8.463 ± 0.084	1.469AlaTrp: 1.469 ± 0.03	2.617AlaTyr: 2.617 ± 0.036	0.003AlaXaa: 0.003 ± 0.001
Cys	1.104CysAla: 1.104 ± 0.025	0.135CysCys: 0.135 ± 0.011	0.576CysAsp: 0.576 ± 0.02	0.525CysGlu: 0.525 ± 0.019	0.357CysPhe: 0.357 ± 0.015	0.996CysGly: 0.996 ± 0.031	0.346CysHis: 0.346 ± 0.024	0.493CysIle: 0.493 ± 0.018	0.282CysLys: 0.282 ± 0.014	0.821CysLeu: 0.821 ± 0.023	0.243CysMet: 0.243 ± 0.011	0.294CysAsn: 0.294 ± 0.014	0.479CysPro: 0.479 ± 0.02	0.259CysGln: 0.259 ± 0.013	0.622CysArg: 0.622 ± 0.021	0.607CysSer: 0.607 ± 0.02	0.521CysThr: 0.521 ± 0.022	0.723CysVal: 0.723 ± 0.024	0.111CysTrp: 0.111 ± 0.01	0.227CysTyr: 0.227 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.69AspAla: 6.69 ± 0.072	0.516AspCys: 0.516 ± 0.02	2.9AspAsp: 2.9 ± 0.048	3.32AspGlu: 3.32 ± 0.052	2.168AspPhe: 2.168 ± 0.035	4.535AspGly: 4.535 ± 0.059	1.072AspHis: 1.072 ± 0.026	3.073AspIle: 3.073 ± 0.044	2.247AspLys: 2.247 ± 0.035	5.199AspLeu: 5.199 ± 0.065	1.447AspMet: 1.447 ± 0.029	1.482AspAsn: 1.482 ± 0.035	2.708AspPro: 2.708 ± 0.046	1.527AspGln: 1.527 ± 0.03	3.216AspArg: 3.216 ± 0.046	2.415AspSer: 2.415 ± 0.037	2.702AspThr: 2.702 ± 0.056	4.194AspVal: 4.194 ± 0.054	0.856AspTrp: 0.856 ± 0.024	1.501AspTyr: 1.501 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.449GluAla: 6.449 ± 0.092	0.493GluCys: 0.493 ± 0.018	2.556GluAsp: 2.556 ± 0.04	3.589GluGlu: 3.589 ± 0.064	2.068GluPhe: 2.068 ± 0.036	3.863GluGly: 3.863 ± 0.049	1.392GluHis: 1.392 ± 0.032	3.303GluIle: 3.303 ± 0.053	2.749GluLys: 2.749 ± 0.045	5.885GluLeu: 5.885 ± 0.062	1.544GluMet: 1.544 ± 0.029	1.721GluAsn: 1.721 ± 0.035	2.254GluPro: 2.254 ± 0.042	2.864GluGln: 2.864 ± 0.053	4.611GluArg: 4.611 ± 0.068	2.952GluSer: 2.952 ± 0.05	2.902GluThr: 2.902 ± 0.042	4.159GluVal: 4.159 ± 0.058	0.817GluTrp: 0.817 ± 0.022	1.292GluTyr: 1.292 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.301PheAla: 4.301 ± 0.053	0.427PheCys: 0.427 ± 0.018	2.462PheAsp: 2.462 ± 0.036	2.006PheGlu: 2.006 ± 0.032	1.494PhePhe: 1.494 ± 0.036	3.558PheGly: 3.558 ± 0.053	0.788PheHis: 0.788 ± 0.024	1.939PheIle: 1.939 ± 0.037	1.374PheLys: 1.374 ± 0.031	3.303PheLeu: 3.303 ± 0.045	0.933PheMet: 0.933 ± 0.021	1.34PheAsn: 1.34 ± 0.034	1.689PhePro: 1.689 ± 0.031	1.065PheGln: 1.065 ± 0.025	2.23PheArg: 2.23 ± 0.039	2.471PheSer: 2.471 ± 0.039	1.968PheThr: 1.968 ± 0.038	2.797PheVal: 2.797 ± 0.044	0.477PheTrp: 0.477 ± 0.019	1.039PheTyr: 1.039 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	7.979GlyAla: 7.979 ± 0.088	0.927GlyCys: 0.927 ± 0.026	3.935GlyAsp: 3.935 ± 0.055	4.342GlyGlu: 4.342 ± 0.056	3.341GlyPhe: 3.341 ± 0.05	6.407GlyGly: 6.407 ± 0.084	1.862GlyHis: 1.862 ± 0.033	4.763GlyIle: 4.763 ± 0.048	4.319GlyLys: 4.319 ± 0.059	7.803GlyLeu: 7.803 ± 0.077	2.644GlyMet: 2.644 ± 0.045	2.689GlyAsn: 2.689 ± 0.057	2.631GlyPro: 2.631 ± 0.042	2.864GlyGln: 2.864 ± 0.045	4.849GlyArg: 4.849 ± 0.057	4.46GlySer: 4.46 ± 0.062	4.611GlyThr: 4.611 ± 0.068	6.128GlyVal: 6.128 ± 0.073	1.11GlyTrp: 1.11 ± 0.03	2.477GlyTyr: 2.477 ± 0.042	0.004GlyXaa: 0.004 ± 0.002
His	2.863HisAla: 2.863 ± 0.043	0.286HisCys: 0.286 ± 0.013	1.236HisAsp: 1.236 ± 0.027	1.242HisGlu: 1.242 ± 0.028	0.988HisPhe: 0.988 ± 0.027	1.986HisGly: 1.986 ± 0.04	0.662HisHis: 0.662 ± 0.024	1.145HisIle: 1.145 ± 0.026	0.683HisLys: 0.683 ± 0.021	2.071HisLeu: 2.071 ± 0.034	0.545HisMet: 0.545 ± 0.018	0.568HisAsn: 0.568 ± 0.021	1.412HisPro: 1.412 ± 0.031	0.722HisGln: 0.722 ± 0.021	1.368HisArg: 1.368 ± 0.03	1.076HisSer: 1.076 ± 0.03	1.053HisThr: 1.053 ± 0.026	1.682HisVal: 1.682 ± 0.035	0.306HisTrp: 0.306 ± 0.013	0.681HisTyr: 0.681 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.924IleAla: 6.924 ± 0.067	0.488IleCys: 0.488 ± 0.017	3.481IleAsp: 3.481 ± 0.047	3.367IleGlu: 3.367 ± 0.052	1.564IlePhe: 1.564 ± 0.033	4.714IleGly: 4.714 ± 0.064	1.01IleHis: 1.01 ± 0.024	2.146IleIle: 2.146 ± 0.042	1.983IleLys: 1.983 ± 0.035	4.369IleLeu: 4.369 ± 0.06	1.053IleMet: 1.053 ± 0.026	1.834IleAsn: 1.834 ± 0.039	2.435IlePro: 2.435 ± 0.04	1.396IleGln: 1.396 ± 0.026	3.151IleArg: 3.151 ± 0.042	2.963IleSer: 2.963 ± 0.048	2.773IleThr: 2.773 ± 0.045	4.173IleVal: 4.173 ± 0.055	0.509IleTrp: 0.509 ± 0.018	1.107IleTyr: 1.107 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.594LysAla: 4.594 ± 0.057	0.228LysCys: 0.228 ± 0.012	2.193LysAsp: 2.193 ± 0.041	2.354LysGlu: 2.354 ± 0.041	1.246LysPhe: 1.246 ± 0.029	2.87LysGly: 2.87 ± 0.047	0.856LysHis: 0.856 ± 0.026	2.056LysIle: 2.056 ± 0.044	1.989LysLys: 1.989 ± 0.039	4.17LysLeu: 4.17 ± 0.055	1.189LysMet: 1.189 ± 0.027	1.388LysAsn: 1.388 ± 0.028	2.34LysPro: 2.34 ± 0.04	1.657LysGln: 1.657 ± 0.036	2.661LysArg: 2.661 ± 0.044	2.214LysSer: 2.214 ± 0.04	2.343LysThr: 2.343 ± 0.044	3.129LysVal: 3.129 ± 0.051	0.468LysTrp: 0.468 ± 0.016	0.884LysTyr: 0.884 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	12.811LeuAla: 12.811 ± 0.111	0.997LeuCys: 0.997 ± 0.024	5.63LeuAsp: 5.63 ± 0.066	5.45LeuGlu: 5.45 ± 0.063	3.673LeuPhe: 3.673 ± 0.062	7.759LeuGly: 7.759 ± 0.091	2.366LeuHis: 2.366 ± 0.041	4.504LeuIle: 4.504 ± 0.053	4.187LeuLys: 4.187 ± 0.052	10.336LeuLeu: 10.336 ± 0.119	2.446LeuMet: 2.446 ± 0.037	3.071LeuAsn: 3.071 ± 0.048	5.467LeuPro: 5.467 ± 0.063	3.681LeuGln: 3.681 ± 0.053	6.751LeuArg: 6.751 ± 0.077	6.31LeuSer: 6.31 ± 0.072	5.322LeuThr: 5.322 ± 0.066	7.413LeuVal: 7.413 ± 0.073	0.972LeuTrp: 0.972 ± 0.027	2.183LeuTyr: 2.183 ± 0.041	0.003LeuXaa: 0.003 ± 0.002
Met	2.932MetAla: 2.932 ± 0.045	0.198MetCys: 0.198 ± 0.012	1.34MetAsp: 1.34 ± 0.032	1.419MetGlu: 1.419 ± 0.032	0.82MetPhe: 0.82 ± 0.025	1.869MetGly: 1.869 ± 0.037	0.653MetHis: 0.653 ± 0.021	1.224MetIle: 1.224 ± 0.025	1.352MetLys: 1.352 ± 0.03	2.94MetLeu: 2.94 ± 0.047	0.735MetMet: 0.735 ± 0.024	1.0MetAsn: 1.0 ± 0.028	1.548MetPro: 1.548 ± 0.034	1.285MetGln: 1.285 ± 0.028	1.89MetArg: 1.89 ± 0.033	1.651MetSer: 1.651 ± 0.033	1.746MetThr: 1.746 ± 0.034	1.846MetVal: 1.846 ± 0.036	0.243MetTrp: 0.243 ± 0.013	0.481MetTyr: 0.481 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.706AsnAla: 3.706 ± 0.059	0.34AsnCys: 0.34 ± 0.015	1.635AsnAsp: 1.635 ± 0.044	1.627AsnGlu: 1.627 ± 0.036	1.11AsnPhe: 1.11 ± 0.028	2.704AsnGly: 2.704 ± 0.047	0.603AsnHis: 0.603 ± 0.02	1.683AsnIle: 1.683 ± 0.034	1.18AsnLys: 1.18 ± 0.027	3.018AsnLeu: 3.018 ± 0.052	0.738AsnMet: 0.738 ± 0.023	1.004AsnAsn: 1.004 ± 0.029	1.96AsnPro: 1.96 ± 0.043	1.033AsnGln: 1.033 ± 0.029	1.968AsnArg: 1.968 ± 0.037	1.434AsnSer: 1.434 ± 0.036	1.667AsnThr: 1.667 ± 0.038	2.359AsnVal: 2.359 ± 0.049	0.407AsnTrp: 0.407 ± 0.015	0.825AsnTyr: 0.825 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	5.818ProAla: 5.818 ± 0.077	0.336ProCys: 0.336 ± 0.014	3.162ProAsp: 3.162 ± 0.053	3.379ProGlu: 3.379 ± 0.046	1.796ProPhe: 1.796 ± 0.034	4.041ProGly: 4.041 ± 0.056	1.103ProHis: 1.103 ± 0.029	1.989ProIle: 1.989 ± 0.039	1.787ProLys: 1.787 ± 0.041	4.66ProLeu: 4.66 ± 0.053	1.174ProMet: 1.174 ± 0.026	1.44ProAsn: 1.44 ± 0.032	2.211ProPro: 2.211 ± 0.039	1.831ProGln: 1.831 ± 0.033	2.472ProArg: 2.472 ± 0.043	2.572ProSer: 2.572 ± 0.041	2.11ProThr: 2.11 ± 0.043	4.19ProVal: 4.19 ± 0.062	0.621ProTrp: 0.621 ± 0.018	1.22ProTyr: 1.22 ± 0.031	0.003ProXaa: 0.003 ± 0.001
Gln	4.558GlnAla: 4.558 ± 0.057	0.303GlnCys: 0.303 ± 0.016	1.641GlnAsp: 1.641 ± 0.033	2.023GlnGlu: 2.023 ± 0.038	1.37GlnPhe: 1.37 ± 0.031	2.6GlnGly: 2.6 ± 0.047	0.892GlnHis: 0.892 ± 0.024	1.79GlnIle: 1.79 ± 0.04	1.463GlnLys: 1.463 ± 0.035	3.765GlnLeu: 3.765 ± 0.054	1.041GlnMet: 1.041 ± 0.026	1.029GlnAsn: 1.029 ± 0.025	1.867GlnPro: 1.867 ± 0.033	1.814GlnGln: 1.814 ± 0.034	2.678GlnArg: 2.678 ± 0.046	2.017GlnSer: 2.017 ± 0.038	1.745GlnThr: 1.745 ± 0.037	2.825GlnVal: 2.825 ± 0.043	0.508GlnTrp: 0.508 ± 0.019	0.876GlnTyr: 0.876 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	6.515ArgAla: 6.515 ± 0.07	0.564ArgCys: 0.564 ± 0.02	3.588ArgAsp: 3.588 ± 0.053	4.187ArgGlu: 4.187 ± 0.062	2.763ArgPhe: 2.763 ± 0.037	3.98ArgGly: 3.98 ± 0.051	1.697ArgHis: 1.697 ± 0.029	3.937ArgIle: 3.937 ± 0.055	2.793ArgLys: 2.793 ± 0.042	6.996ArgLeu: 6.996 ± 0.087	2.081ArgMet: 2.081 ± 0.036	2.192ArgAsn: 2.192 ± 0.045	2.727ArgPro: 2.727 ± 0.042	2.614ArgGln: 2.614 ± 0.043	4.378ArgArg: 4.378 ± 0.064	3.221ArgSer: 3.221 ± 0.049	3.194ArgThr: 3.194 ± 0.049	4.531ArgVal: 4.531 ± 0.05	0.897ArgTrp: 0.897 ± 0.022	1.836ArgTyr: 1.836 ± 0.036	0.003ArgXaa: 0.003 ± 0.001
Ser	6.176SerAla: 6.176 ± 0.063	0.559SerCys: 0.559 ± 0.022	2.785SerAsp: 2.785 ± 0.046	2.868SerGlu: 2.868 ± 0.045	2.224SerPhe: 2.224 ± 0.037	5.385SerGly: 5.385 ± 0.077	1.255SerHis: 1.255 ± 0.029	2.932SerIle: 2.932 ± 0.05	2.014SerLys: 2.014 ± 0.036	5.722SerLeu: 5.722 ± 0.063	1.593SerMet: 1.593 ± 0.03	1.626SerAsn: 1.626 ± 0.04	2.65SerPro: 2.65 ± 0.042	1.83SerGln: 1.83 ± 0.034	3.496SerArg: 3.496 ± 0.053	3.289SerSer: 3.289 ± 0.059	2.966SerThr: 2.966 ± 0.048	4.082SerVal: 4.082 ± 0.055	0.681SerTrp: 0.681 ± 0.021	1.35SerTyr: 1.35 ± 0.033	0.001SerXaa: 0.001 ± 0.001
Thr	5.778ThrAla: 5.778 ± 0.069	0.472ThrCys: 0.472 ± 0.019	2.704ThrAsp: 2.704 ± 0.044	2.694ThrGlu: 2.694 ± 0.046	1.914ThrPhe: 1.914 ± 0.039	4.891ThrGly: 4.891 ± 0.068	1.163ThrHis: 1.163 ± 0.029	2.792ThrIle: 2.792 ± 0.043	1.597ThrLys: 1.597 ± 0.032	5.774ThrLeu: 5.774 ± 0.073	1.292ThrMet: 1.292 ± 0.027	1.465ThrAsn: 1.465 ± 0.033	3.029ThrPro: 3.029 ± 0.051	1.783ThrGln: 1.783 ± 0.041	3.116ThrArg: 3.116 ± 0.05	2.875ThrSer: 2.875 ± 0.055	2.928ThrThr: 2.928 ± 0.057	4.271ThrVal: 4.271 ± 0.056	0.638ThrTrp: 0.638 ± 0.02	1.271ThrTyr: 1.271 ± 0.031	0.001ThrXaa: 0.001 ± 0.001
Val	8.673ValAla: 8.673 ± 0.083	0.746ValCys: 0.746 ± 0.022	4.157ValAsp: 4.157 ± 0.057	4.482ValGlu: 4.482 ± 0.061	2.841ValPhe: 2.841 ± 0.048	5.304ValGly: 5.304 ± 0.064	1.607ValHis: 1.607 ± 0.031	3.941ValIle: 3.941 ± 0.053	2.971ValLys: 2.971 ± 0.05	7.723ValLeu: 7.723 ± 0.091	2.08ValMet: 2.08 ± 0.037	2.529ValAsn: 2.529 ± 0.04	3.789ValPro: 3.789 ± 0.06	2.49ValGln: 2.49 ± 0.039	4.944ValArg: 4.944 ± 0.054	4.344ValSer: 4.344 ± 0.06	4.384ValThr: 4.384 ± 0.059	6.252ValVal: 6.252 ± 0.078	0.899ValTrp: 0.899 ± 0.027	1.636ValTyr: 1.636 ± 0.034	0.001ValXaa: 0.001 ± 0.001
Trp	1.001TrpAla: 1.001 ± 0.025	0.147TrpCys: 0.147 ± 0.009	0.572TrpAsp: 0.572 ± 0.019	0.615TrpGlu: 0.615 ± 0.02	0.552TrpPhe: 0.552 ± 0.023	0.817TrpGly: 0.817 ± 0.026	0.341TrpHis: 0.341 ± 0.014	0.723TrpIle: 0.723 ± 0.021	0.568TrpLys: 0.568 ± 0.02	1.602TrpLeu: 1.602 ± 0.041	0.435TrpMet: 0.435 ± 0.016	0.471TrpAsn: 0.471 ± 0.018	0.512TrpPro: 0.512 ± 0.02	0.61TrpGln: 0.61 ± 0.023	0.909TrpArg: 0.909 ± 0.026	0.694TrpSer: 0.694 ± 0.023	0.695TrpThr: 0.695 ± 0.023	0.812TrpVal: 0.812 ± 0.024	0.196TrpTrp: 0.196 ± 0.013	0.313TrpTyr: 0.313 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.542TyrAla: 2.542 ± 0.043	0.285TyrCys: 0.285 ± 0.014	1.322TyrAsp: 1.322 ± 0.03	1.241TyrGlu: 1.241 ± 0.028	1.111TyrPhe: 1.111 ± 0.029	2.111TyrGly: 2.111 ± 0.041	0.547TyrHis: 0.547 ± 0.018	1.054TyrIle: 1.054 ± 0.026	0.873TyrLys: 0.873 ± 0.022	2.581TyrLeu: 2.581 ± 0.045	0.524TyrMet: 0.524 ± 0.018	0.713TyrAsn: 0.713 ± 0.024	1.26TyrPro: 1.26 ± 0.033	0.948TyrGln: 0.948 ± 0.027	1.861TyrArg: 1.861 ± 0.04	1.363TyrSer: 1.363 ± 0.029	1.316TyrThr: 1.316 ± 0.029	1.765TyrVal: 1.765 ± 0.037	0.398TyrTrp: 0.398 ± 0.017	0.714TyrTyr: 0.714 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.038XaaXaa: 0.038 ± 0.007

Statistics based on 5003 proteins (1573312 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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